The present study was carried out in atheroscleorotic New Zealend white(NZW) rabbits. to evaluate the effect of dietary supplementation with Korean pinenut oil, on plasma total fatty acid composition. In study I, NZW rabbits were fed 10 weeks on a commercial chow diet supplemented with 5% of energy as fats(soybean oil or pinenut oil) or 10% of energy as fats(soybean oil or pinenut oil) with the addition of 1% cholesterol to the diet. Nineteen fatty acids ranged from myristic acid (14:0) to cervonic acid (22:6 Ω3) were identified in all the samples. The c5, c9, c12~18 : 3 acid was not reported in the fatty acid methyl ester profiles of each group because it was included in the linoleic acid peak. The major constitutent fatty acids in the chow diet group were linoleic acid, oleic acid, palmitic acid and α-linolenic acid. In the cholesterol group, oleic acid, linoleic acid and palmitic acid were the major fatty acids. In plasma of cholesterol-fed animals, the levels of 16:1 Ω 7 and 18:1 1 Ω 9 were increased. Linoleic acid was the major fatty acid in soybean oil/cholesterol and pinenut oil/cholesterol groups. Plasma linoleic acid levels were significantly incresed from 4 to 6% by the supplementation of 5% soybean or 5% pinenut oil in the cholesterol diet for 5 weeks, compared to cholesterol group. Plasma 16 : 1 Ω 7 levels in animals fed with 5 or 10% pinenut oils were significantly lower than in those fed cholesterol for 5 weeks. After 10 weeks on the soybean oil and pinenut oil diet there were no significant differences in the fatty acid composition. In study II, the fatty acid composition was not affected by the types or levels of oils supplemented for 5 weeks. After 10 weeks on the oil diets 16:1 Ω 7 and 18:1 Ω 9 were decreased in 10% soybean in oil/cholesterol and 10% pinenut oil/cholesterol groups, compared to cholesterol group.

The present study was undertaken to determine the optimal condition for parthenogenetic activation of rabbit oocytes by electric stimulation in vitro in an attempt to develop nuclear transplantation techniques for cloning mammalian embryos and animals. Freshly ovulated oocytes were collected from superovulated rabbits from 13 to 26 hrs. after hCG injection. The cumulus-free oocytes were activated parthenogetically by repeated stimuli of square direct electric pulses in O.3M mannitol solution. After applying electric stimulations of different voltages, pulse durations and pulse times, all of the oocytes were cultured in TCM-199 with 10% FCS for 96 hours in a 5% incubator, and their developmental potential in vitro was examined. The higher activation rate (68.9%) was achieved at the voltage of 2.0kv/cm, the pulse duration of 60 sec and three pulse times and the activation rate of 100% was achieved at the pulse duration of 100 and 200 sec, the voltage of 1.5kv/cm and three pulse times. Although the higher rates of activation of oocytes were achieved at 100 and 200 sec, none of them developed to blastocyst in vitro. The oocytes collected 18~20 hours post hCG injection showed the highest rate of activation and development to blastocyst in vitro than the oocytes collected 13~15 or 25~26 hours post hCG injection. Therefore, it can be suggested that the application of electric stimulation of 2.0kv/cm, 60 sec and three pulse times to the oocytes collected at 18~20 hours post hCG injection would be more beneficial for the parthenogenetic activation of oocytes in rabbits.

같은 천남성과에 속하면서도 정유의 함유성분 및 함량이 서로 다른 수창포 정유와 석창포 정유가 토끼 적출장관 운동에 미치는 영향과 차이점, 또 어떠한 작용양식에 의하여 이루어지는가를 알아보고저 carbachol pilocarpine, histamine 및 barium chloride등과의 상호작용을 비교, 검토하였던 바 다음과 같은 결론을 얻었다. 1. 수창포 정유와 석창포 정유는 모두 토끼 적출장관 운동을 이완시켰으며, 농도를 증가시킬수록 이완정도는 현저하였고, 장관운동의 이완되는 정도는 두 정유 모두 같은 농도에서는 거의 비슷하였다. 2. ED50에 해당하는 농도는 수창포 정유는 0.0045% 이었고, 석창포 정유는 0.0035%이었다. 3. 수창포 정유와 석창포 정유에 의해 이완된 토끼의 적출장관은 carbachol pilocarpine, barium chloride에 의하여 정유 투여전의 상태로 회복하였으나, histamine에 의해서는 부분적인 회복을 보였다. 4. 수창포 정유와 석창포 정유는 carbachol pilocarpine, histamine 및 barium chloride에 의하여 수축된 토끼의 적출장판을 이완시켰다. 5. 이상의 결과로 보아서 수창포 정유와 석창포 정유는 다같이 향신경성작용 및 향근육성작용에 의해 토끼 적출장관 운동을 이완시킨다고 사료된다.

Choline과 choline esters인 acetylcholine, methacholine, carbachol, bethanechol이 토끼의 적출장관 운동에 미치는 영향과 각 약물의 값을 산출하여 각각 비교를 하였다. Choline은 최대유효농도가 이었으며 은 이었고 값은 2.619이었다. Acetylcholine은 최대유효농도가 이었으며 에서는 거의 효과가 나타나지 않았고 은 이었고 값은 5.154이었다. Methacholine은 최대유효농도가 이었으며 에서는 거의 효과가 나타나지 않았고 은 이었고 값은 6.045이었다. Carbachol은 최대유효농도가 이었으며 에서는 거의 효과가 나타나지 않았고 은 이었고 값은 6.244이었다. Bethanechol은 최대유효농도가 이었으며 에서는 거의 효과가 나타나지 않았고 은 이었고 값은 5.480이었다. Choline esters는 토끼의 적출장관 운동에 대하여 수축을 일으켰으며 각 약물의 potency의 순서는 carbachol이 가장 강하게 나타났고 methacholine, bethanechol, acetylcholine, choline의 순으로 나타났다.

Effect of perilla oil on the fatty acid composition, ACAT and HMG-CoA reductase in the liver microsomes, or cholesterol and protein in serum of rabbit were examined. 1. The content of total protein in serum was almost same amount of both groups, but α1-globulin and r-globuline were incresed or β-globulin was decresed compared with control. 2. The content of high density lipoprotein incresed, and the content of low density lipoprotein decresed in lipoprotein. 3. Total cholesterol and triglyceride were decresed, and the content of phospholipid was incresed. 4. Perilla oil did not effect for changing blood glucose and Na+, K+ electrolytes. 5. Perilla oil did not effect for changing serum GOT and GPT in rabbit. 6. The activity of ACAT decresed and the activity of HMG-CoA reductase incresed. The activity of ACAT and HMG-CoA reductase in liver microsomes were reciprocal. 7. There were arachidonic acid 20:4, eicosapentaenoic acid 20:5, and docosahexaenoic acid 22:6 in the liver microsomes of rabbits. These highly polyunsaturated fatty acids were convented from linolenic acid 18:3 n-3.

This study was conducted to investigate the effect of unilateral ovariectomy on the embryo survival and changes of plasma progesterone and estradiol concentration. Mature Angora rabbits were divided into 2 groups (Superovulation and unilateral ovariectomy after superovulation). Unilateral ovariectomied rabilts were subdivided into two groups according to the time of ovariectomy (24 hours and 96 hours after mating). The results obtained were summarized as follows; 1. After unilateral ovariectomy, survival rate and collecting rate of embryos recovered from contralateral and ipsilateral oviduct and uterus were not different, and were lower than intact rabbits. 2. Plasma progesterone concentration at 93, 99, 102 and 114 hours after HCG injection in superovulated rabbits were 12.9 0.5, 34.8 5.1, 12.2 2.7 and 43.4 5.8ng/ml, respectively. Mean progesterone concentrations were significantly higher at 99 and 114 hours than at 93 and 102 hours (p <0.05). But plasma estradiol concentration was not different. 3. Plasma progesterone concentration in unilateral ovariectomized rabbits was somewhat decreased after unilateral ovariectomy. Plasma estradiol concentration was not different.

식물에서 얻어지는 정유의 한 성분으로 화학구조가 유사한 anethole, eugenol, isoeugenol, safrole 및 isosafrole이 토끼 적출장관 운동에 미치는 영향과 그 potency를 알아 보고 또 어떤 작용 양식에 의하여 이루어 지는가를 알아보고 carbachol, pilocarpine, barium chloride 및 histamine 등과의 상호작용을 검토하였던 바 다음과 같은 결론을 얻었다. 1. 화학구조가 유사한 다섯가지 정유들을 단독 투여하였던 바 각각 억제 정도에 차이는 있었으나 모두 토끼 적출장관 운동을 억제시켰으며 그 potency는 isoeugenol>isosafrole>eugenol>safrole>anethole의 순으로 그 값은 각각 4.22, 4.18, 4.17, 4.15 및 3.82이었다. 2. 실험에 사용한 정유 모두가 carbachol, pilocarpine, barium chloride 및 histamine에 의하여 수축된 장관을 이완시켰다. 3. Anethole에 의하여 이완된 장관은 carbachol, pilocarpine, barium chloride 및 histamine에 의하여 모두 투약전과 같은 상태로 회복하지 못하였으며 eugenol, isoeugenol, safrole 및 isosafrole에 의하여 이완된 장관은 carbachol, pilocarpine 및 barium chloride에 의하여는 투약전의 상태로 회복하였으나 histamine으로는 부분적인 회복을 나타내었다. 4. 이상의 결과로 보아서 실험에 사용한 정유들은 향신경성(neurotropic) 및 향근육성(musculotropic), 양쪽에 작용하여 토끼의 적출장관 운동을 억제시킨다고 생각된다.

인삼, 들깨 기름, 칼슘 및 마그네슘이 함유되어 있는 식이로 사육한 토끼의 혈장 콜레스테롤과 HMG-CoA reductase의 활성을 조사하였다. 간에 포함되어 있는 HMG-CoA reductase의 활성은 인삼과 들깨 기름 및 마그네슘 첨가 식이군에 있어서 증가되었으며 혈장 콜레스테롤의 농도는 감소되었다. 혈장 LDL-콜레스테롤은 대조군보다 감소하였으나 HDL-콜레스테롤은 증가되었다. 또한 베타 글로블린의 분포도 인삼과 마그네슘 및 들깨 기름을 첨가 급여한 실험군에 있어서 가장 낮았으며, α-2-글로블린은 대조군에 비하여 가장 높은 값을 보였다. 전해질은 K+은 인삼 첨가로 감소 되었으나 Na+은 증가되었다. 혈장 마그네슘의 농도는 인삼의 첨가로 변화가 없었지만 칼슘과 들깨 기름 및 인삼 첨가식이로 마그네슘의 농도가 더 증가되었다. 혈장 칼슘의 농도는 마그네슘과 인삼을 첨가한 식이군에 있어서 감소하였다.

This paper intends to reveal that Eliot’s life entered a profound influence upon his earlier poems, especially in the unpublished work, Inventions of the March Hare: Poems 1909-1917, which was edited by Christopher Ricks in 1996. Eliot said that there might be the experience of a child of ten, a small boy peering through sea-water in a rock-pool, and finding a sea-anemone for the first time: the simple experience (not so simple, for an exceptional child, as it looks) might lie dormant in his mind for twenty years, and re-appear transformed in some verse-context charged with great imaginative pressure. This paper deals with the personal experiences of his early years, from his boyhood to the time when he returned for the autumn term of 1911 and enrolled as a graduate student in philosophy; that is, the inhibiting circumstances from Unitarianism, the emotional conflicts between him and his parents from preparing for Harvard University, and from his mother’s opposition to studying abroad in Paris in 1910. In his poetry, Eliot reveals his passion for studying abroad in Paris that he kept in his mind even before graduation from Harvard University, and he also expresses his circumstances through his poems indirectly, something which he would not dare communicate directly to his strict family. For example, in the situation that he already had lost interest in Boston, the streets already seemed so boring and also it seemed the “Mandarins” says the oppressed feelings of his family that had been against the abroad study. He also expresses himself as a clownesque or a marionette, which even can’t make any decision about themselves.