2024년 1월 1일 발생한 규모 7.5의 일본 노토지진은 강력한 지반 운동, 지진 해일과 함께 대기 압력 변동에 의 한 인프라사운드 신호를 동반하였다. 노토지진 인프라사운드 신호는 진앙에서 약 830 km 떨어진 국내 인프라사운드 관측소 뿐만 아니라 약 3,500 km 떨어진 국제관측망에서도 탐지되었다. 지진 발생 당시 성층권과 중간권 고도에 형성된 동-서 반대 방향의 강한 바람은 동북아시아 지역에 위치한 관측소와 반대 방향인 태평양에 위치한 관측소에서 지진 인 프라사운드 신호 관측을 가능하게 하였다. 역-투사 방법으로 음원의 발생지역을 계산한 결과, 고주파수 대역(1-2 Hz)의 인프라사운드 신호는 주로 진앙을 포함한 일본 내륙의 지반 운동으로 발생했으며, 저주파수 인프라사운드(0.3 Hz 이하) 는 지진과 동시에 발생한 일본 서쪽 해안의 지진 해일(해수면 운동)에 의해 발생한 것으로 해석된다. 지표면-대기 상호 작용 관계식과 지진 해일 경보 높이(3 m)로 추정한 지진 해일 기원의 인프라사운드 음원 크기는 약 120 Pa로 육지 지 반 운동에 의한 음원의 크기(약 280 Pa)와 비교될 만한 것으로 확인되었다. 2024년 노토지진은 지구 표면의 지반 운동 과 해수면 운동에 의한 인프라사운드 신호가 다중 관측소에서 동시에 관측된 사례로 평가된다.
On the wooden slabs unearthed from the tomb of Guojianao (formerly, Guo) in the period of Sun Wu of the Three Kingdoms, there is a sentence with the words “針囊一枚, 白糸一 , 青糸一”. Past scholars interpreted the three characters “ ” ,“ ” and “ ” as the word “縱 (zong)” , which means “silk thread”, and “ ” ,“ ” as the word “㢧 (卷, juan, volume)”, which is a measure word. This study verifies that the word “ ” is “ ”, and “ ”, “ ” are “ ”, which are all variants of the word “線 (xian, line)”. This was achieved by changing the phonetic note. The measure word “ ”, “ ” used for weighing silk thread should be a variant form of the word “斤 (jin)”, which is similar to the writing of “ (jin)” or “ (jin)” in the Pan’s clothing list of the Eastern Jin Dynasty.
Spodoptera litura Fabricius, 1775 (Lepidoptera: Noctuidae) is a serious crop pest with a long-distance migratory flight. To date, the DNA barcode region has been widely used in genetic diversity analysis studies of Spodoptera litura. However, the DNA barcode region showed maximum variation rate of S. litura, which from 18 regions in South Korea, was 0.608% (nine haplotypes) in previous study. In this study, four mitochondrial genes (ND4, ND4L, ND1, 16s rRNA) have higher intra-specific variation rates than the DNA barcode region. Among the four genes, The variation rate of the 16s rRNA region was confirmed to be a minimum of 0.203% (2bp) and a maximum of 1.824% (18bp). Finally, the 16s rRNA region with the highest PCR amplification efficiency and highest variation rate was selected as a high-efficiency molecular marker.
미토콘드리아 시토크롬 c 산화효소 1 (COX1) 유전자 염기서열(658 bp)을 사용하여, 콩 포장에서 채집된 어리팥나방(Matsumuraeses falcana)과 팥나방(Matsumuraeses phaseoli)의 종을 실험실 집단의 종들과 비교하여 동정하였다. COX1 염기서열 분석에서, 어리팥나방 47개체 로부터 10개의 하플로타입이 발견되었고, 종내 유전적 거리는 0.15~0.46%이었다. 이중 하프로타입 A형이 약 70%로 우점형이었다. 팥나방의 30개체로부터는 모두 동일한 하나의 서열만이 확인되었고, 어리팥나방과의 종간 유전적 거리는 4.11~4.61%이었다. 두 종의 COX1 염기서열을 번역한 아미노산 서열은 모두 동일하여 동의적 염기서열 변이(동의치환, 同義置換, synonymous substitution)를 확인할 수 있었다. 포장 조사에 서 두 종의 유충이 콩의 잎과 꼬투리를 가해하였고, 한 포장에서 동시에 발생하였다. 전체 포장에서 어리팥나방의 평균 밀도는 팥나방보다 약 1.5 배 높았다. 이 결과는 콩이 두 종의 동일 기주임을 명백하게 제시하였다. 별도로 이 속의 유충 기생파리로서 Elodia flavipalpis (파리목: 기생파리 과)가 발견되었고, COX1 서열로 동정되었다.
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an N6-methyladenosine (m6A) RNA modification regulator and a key determinant of premRNA processing, mRNA metabolism and transportation in cells. Currently, m6A reader proteins such as hnRNPA2/B1 and YTHDF2 has functional roles in mice embryo. However, the role of hnRNPA2/B1 in porcine embryogenic development are unclear. Here, we investigated the developmental competence and mRNA expression levels in porcine parthenogenetic embryos after hnRNPA2/B1 knock-down. HhnRNPA2/B1 was localized in the nucleus during subsequent embryonic development since zygote stage. After hnRNPA2/B1 knock-down using double stranded RNA injection, blastocyst formation rate decreased than that in the control group. Moreover, hnRNPA2/B1 knock-down embryos show developmental delay after compaction. In blastocyste stage, total cell number was decreased. Interestingly, gene expression patterns revealed that transcription of Pou5f1, Sox2, TRFP2C, Cdx2 and PARD6B decreased without changing the junction protein, ZO1, OCLN, and CDH1. Thus, hnRNPA2/B1 is necessary for porcine early embryo development by regulating gene expression through epigenetic RNA modification.
Recently, M/G/1 priority queues with a finite buffer for high-priority customers and an infinite buffer for low-priority customers have applied to the analysis of communication systems with two heterogeneous traffics : delay-sensitive traffic and loss-sensitive traffic. However, these studies are limited to M/G/1 priority queues with finite and infinite buffers under a work-conserving priority discipline such as the nonpreemptive or preemptive resume priority discipline. In many situations, if a service is preempted, then the preempted service should be completely repeated when the server is available for it. This study extends the previous studies to M/G/1 priority queues with finite and infinite buffers under the preemptive repeat-different and preemptive repeat-identical priority disciplines. We derive the loss probability of high-priority customers and the waiting time distributions of high- and low-priority customers. In order to do this, we utilize the delay cycle analysis of finite-buffer M/G/1/K queues, which has been recently developed for the analysis of M/G/1 priority queues with finite and infinite buffers, and combine it with the analysis of the service time structure of a low-priority customer for the preemptive-repeat and preemptive-identical priority disciplines. We also present numerical examples to explore the impact of the size of the finite buffer and the arrival rates and service distributions of both classes on the system performance for various preemptive priority disciplines.
Cell adhesion plays an important role in the differentiation of the morphogenesis and the trophectoderm epithelium of the blastocyst. In the porcine embryo, CDH1 mediated adhesion initiates at compaction before blastocyst formation, regulated post-translationally via protein kinase C and other signaling molecules. Here we focus on muscle RAS oncogene homolog (M-RAS), which is the closest relative to the RAS related proteins and shares most regulatory and effector interactions. To characterize the effects of M-RAS on embryo compaction, we used gain- and loss-of-function strategies in porcine embryos, in which M-RAS gene structure and protein sequence are conserved. We showed that knockdown of M-RAS in zygotes reduced embryo development abilities and CDH1 expression. Moreover, the phosphorylation of ERK was also decreased in M-RAS KD embryos. Overexpression of M-RAS allows M-RAS KD embryos to rescue the embryo compaction and blastocyst formation. Collectively, these results highlight novel conserved and multiple effects of M-RAS during porcine embryo development.
As buildings are becoming larger, demand for mega-sized composite columns (over 1-meter diameter) is increased. We have developed and commercialized welded built-up CFT column (ACT Column I) since 2005 which are structurally stable and economical using cold-formed steel with rib. However, there has a limit in size of cross section (618˟618mm) by a fabrication facilities. And due to characteristics of closed cross section, there has a limit to construction of connection of moment frame. Composite mega column (ACT Column II) has same concept of forming closed cross section. But in order to enlarge cross sectional size, thick plate is inserted between cold-formed steels. Since composite mega column can control thickness and width of thick plate, steel or composite beams can be directly attached to the connection. In this study, we propose strength formula of composite mega column to beam connections with T-shaped stiffener as internal diaphragm and verified through finite element analysis and simple tensile experiment.
The utilization of composite columns is increasing due to the construction of high-rise buildings and large buildings. The commercially available concrete chimney steel column (ACT I) is a stable and economical structure, but there is a limit in the section size to be applied to a composite column subjected to a high load. We have developed a composite mega column with an integral structure by adding a plate to the central part of the ACT I column and installing a binding frame at a certain interval inside the central plate. In this study, to evaluate the compressive performance of the composite mega column, four test specimens were constructed with binding frame reinforcement, reinforcement spacing, and reinforced cross - sectional area. The structural performance of the composite section is compared with that of KBC2016 to evaluate the behavior of the specimen.