본 연구는 성인 학습자의 노후 준비도가 노인부양의식에 미치는 영향요인을 파악하기 위함이며, 2023년 4월 13일부터 6월 29일까지 K 사이버대학교 성인 학습자 147명을 분석대상으로 삼았다. 수집된 자료는 분산분석, 단별량 및 다 별량 회귀분석, 상관관계 분석을 사용하였다. 분석결과 성인 학습자의 노후준비 도 영향요인 차이는 성별, 연령별에서 차이가 나타났고(p<0.05), 노후 준비도 에 따른 노인 부양의식 요인에 미치는 영향은 성인 학습자들의 경우 더욱 관심을 갖고 있는 것으로 나타나 통계적으로 유의한 것으로 나타났다 (P<0.05). 노후 준비도와 노인부양의식과의 상관관계에서는 결정계수 R값 은 경제적 부양요인과 정서적 부양요인(0.717), 신체적 부양요인(0.779)은 관계성이 높게 나타났다. 이는 노후준비를 어떻게 하느냐에 따라서 노인부 양의식과 노후의 삶이 영향을 미치고 있어 철저한 사전 노후준비와 긍정적 인 노인부양의식에 대한 변화 유도, 그리고 종합적이고 기초적인 유용한 자료 로 활용하는 데 함의가 있다.

The purpose of the study was to investigate the effects of sea cucumber extract on skin as a natural cosmetics functional material. Subjective evaluation of cosmetics before and after were conducted with questionnaires regarding moisture content, sebum content, melanin index, and erythema index. Experiments were conducted on improvement efficacy using skin clinical trials and questionnaires to evaluate changes in perception of skin condition and efficiency of products. With the aim of minimizing skin irritation, the efficiency of the solvent used for extraction was an important factor, and the sea cucumber extract was harvested with efficient extraction conditions at a ratio of 1:10 of 50% ethanol. The study aimed to identify the suitability of sea cucumber extract as a functional cosmetics material to improve the moisturizing ability of skin and its effect on the skin by adding marine natural animal sea cucumber extract. Clinical studies on cosmetics skin containing sea cucumber extract, excellent skin improvement effect from all items of clinical experiment in experimental and control groups. Sea cucumber extract was proved to be a stable, non-adverse physiologically active substance against abnormal symptoms or side effects of skin reactions and skin problems. In addition, the study found excellent results that can lead to its use as a cosmetics material. This is expected to contribute to the development of various cosmetics industries.

목적 : 본 연구는 국내 건강한 성인을 대상으로 구강안면근력을 측정하여 표준치를 제시하고 성별, 연령에 따른 근력 변화에 대해 알아보고자 하였다. 연구방법 : 2012년 5월부터 2013년 1월까지 20~89세의 건강한 성인 341명을 대상으로 아이오와 구강 수행도구(Iowa Oral Performance Instrument; IOPI)를 사용하여 구강안면근력을 측정하였다. 측정부위는 구강안면근으로 혀의 전·후방 거상, 좌·우측 볼근, 입술로 각각 3회 측정 후 그 평균값을 측정하였다. 결과 : 1) 성별에 따른 건강한 성인 구강안면근력의 차이는 남성이 여성에 비해 강한 근력을 보였다(p<.01).2) 연령이 증가할수록 구강안면근력은 유의하게 저하된 것을 알 수 있었다(p<.01). 결론 : 본 연구를 통해 구강안면근력의 표준치를 알아보았으며 성별, 연령에 따른 근력 변화는 삼킴을 치료하는데 있어 기본적 지침으로 환자의 현재 상태를 파악하는 것은 물론 나아가 치료방향을 고려하는데 있어 도움을 줄 수 있을 것이다.

This study was designed to investigate the changes in the properties of the neuronal setm cells or progenitor cells associated with age-related decline in neurogenesis of the hippocampal dentate gyrus (DG). Active whole cells cycle marker Ki67 (a marker of whole cell cycle)-positive and S phase marker bromodeoxyuridine (BrdU)-positive. Neural stem cells gradually were reduced in the hippocampal subgranular zone (SGZ) in an age-dependant manner after birth (from P1 month to P1 year). The ratio of BrdUpositivecells/Ki67-positive cells was gradually enhanced in an age-dependent manner. The ratio of Ki67-positive cells/accu-mulating BrdU-positive cells at 3 hrs after BrdU injection was injected once a day for consecutive 5 days gradually decreased during ageing. TUNEL- and caspase 3 (apoptotic terminal caspase)-positive cells gradually decreased in the dentate SGZ during ageing and immunohistochemical findings of glial fibrillary acid protein (GFAP) were not changed during ageing. NeuN, a marker of mature neural cells, and BrdU-double positive cells gradually decreased in an age-dependent manner but differentiating ratio and survival rate of cells were not changed at 4 wks after BrdU injection once a day for consecutive 5 days. The number of BrdU-positive cells migrated from the hippocampal SGZ into granular layer and its migration speed was gradually declined during ageing. These results suggest that the adult neurogenesis in the mouse hippocampal DG gradually decrease through reducing proliferation of neural stem cells accompanying with cells cycle change and reduced cells migration rather than changes of differentiation.

The purpose of this study was to obtain baseline data of nutritional management for women's bone health according to age. This study was conducted through questionnaire, bone mineral density(BMD) measurement by DEXA and dietary survey by 24hr recalls to 134 adult women. Subjects were divided into three groups: young women group who aged 20-29(n=48), middle aged women group who aged over 30 and were pre-menopause(n=36), postmenopausal women group(n=48). The body fat percent of postmenopausal women group was the highest among the three groups. BMD of lumbar spine(L1-L2) were 0.93 g/cm2 for young women, 0.97 g/cm2 for middle aged women, and 0.88 g/cm2 for postmenopausal women, respectively(p〈0.05). BMD of femoral neck(p〈0.05), trochanter(p〈0.05), and ward's (p〈0.001) were significantly decreased as age increasement. Drinking alcohol and skipping meals were significantly higher in young women groups than in the other groups, however frequency of doing regular exercise was significantly lower in young women groups. Young women group consumed the significantly higher amount of cereals(p〈0.05), sugar and sweeteners(p〈0.001), meats(p〈0.01), eggs(p〈0.01), milks(p〈0.05) and oils(p〈0.001) than middle aged and postmenopausal women. Also, energy(p〈0.001), animal protein, (p〈0.01), plant oil(p〈0.001), animal fat(p〈0.001), retinol(p〈0.001), vitamin E(p〈0.01), and cholesterol(p〈0.001) intakes of young women were highest among the three groups. However, young women group consumed the significantly lower amount of dietary fiber(p〈0.05), vitamin C(p〈0.01), folate(p〈0.05) and fruits(p〈0.01) than the other groups. To summarized the our results, young women who were in still undergoing bone formation activity to develope peak bone mass, had more dietary habit and lifestyle problems than middle aged and postmenopausal women. The results of this study revealed that nutritional management and education for bone health should be emphasized in young women not only aged women.

본 연구는 서로 다른 수명 패턴을 갖고 있는 누에성충 중 두가지 타입을 이용하여 수행되었다. 장명품종(성충 수명이 15일 이상, LLS), 단명 품종(성충 수명이 5일 미만, SLS). 누에성충의 노화 생리를 밝히기 위해 장단명 암수에 있어서의 지방체 미세구조를 비교해 보았다. 단명품종에 있어서는, 암컷에는 조면소포체 및 글리코겐 과립이 세포질내에서 다량 확인된 반면, 수컷에는 활면소포체만이 세포질내에서 발견되었다. 또한 단명품종에 있어서는, 성충 3일째 이후 미토콘드리아의 용적이 비대해지는 경향을 보였으며, 많은 지방구 퇴화가 관찰되었다. 단명품종에 반해 장명품종에서는, 성충 5일째의 암컷은 비교적 정상적인 미토콘드리아와 핵막이 관찰되었다. 성충 15일째에 이르러서야 대부분의 세포막이 사라졌고 미토콘드리아가 비정상적으로 비대해졌다. 장명품종 수컷(성충 10일째)의 세포질내에서 다량의 지방과립이 관찰되었으며, 이 개체는 성충 15일째에 세포내용물이 모두 고갈되어 사망하였다. 따라서, 금후 수명에 관한 조직 연구를 위해서는 조직의 변화상을 관찰하기 용이한 단명품종이 적절할 것으로 사료된다.

The present research was chiefly designed to determine the effect of the treatment of estrogenic agonist, estradiol benzoate (EB), or antiandrogenic compound, flutamide (Flu), at the weaning age on the expression of connexin (Cx) isoforms in the caudal epididymis of adult male rat. Animals were subcutaneously administrated with a single shot of either EB at a low-dose (0.015 mg of EB/kg body weight (BW)) or a high-dose (1.5 mg of EB/kg BW) or Flu at a low-dose (500 mg of EB/kg BW) or a high-dose (5 mg of EB/kg BW). Expressional changes of Cx isoforms in the adult caudal epididymis were examined by quantitative real-time PCR analysis. The treatment of a low-dose EB caused significant increases of Cx30.3, Cx31, Cx32, and Cx43 transcript levels but reduction of Cx31.1, Cx37, and Cx45 expression. Exposure to a high-dose EB resulted in very close responses observed in a low-dose EB treatment, except no significant expressional change of Cx37 and a significant induction of Cx40. Expression of all Cx isoforms, except Cx45, was significantly increased by a low-dose Flu treatment. Expressional increases of all Cx isoforms were detected by a high-dose Flu treatment. The current study demonstrates that a single exposure to estrogenic or antiandrogenic compound during the early postnatal developmental period is sufficient to disrupt normal expression of Cx isoforms in the adult caudal epididymis.

Direct communication between neighboring cells via gap junction in tissue is important for maintenance and regulation of its physiological functions. Each epididymal region has different composition of cell types. It is well recognized that the epididymis is a steroid hormone-responsive tissue. The present study was designed to determine the effect of estradiol benzoate (EB) or flutamide exposured at the early postnatal age on the expression of connexin (Cx) isoforms in the caudal epididymis. The EB or flutamide was subcutaneously administrated to male Spragure Dawley rat at 7 days of age, and expressional changes of Cx isoforms in the adult corpus epididymis were determined by quantitative real-time PCR. The treatment of low-dose EB resulted in decreases of Cx30.3, Cx31.1, Cx37, and Cx45 expression but caused an increase of Cx32 expression. Exposure to high-dose EB led into expressional increases of Cx31, Cx31.1, Cx32, Cx40, and Cx43, even though a decrease of Cx37 expression was found with a high-dose EB treatment. A low-dose flutamide induced increases of Cx31, Cx31.1, Cx32, and Cx43 expression but a decrease of Cx37 expression. Expression of most Cx genes were significantly increased by a high-dose flutamide, while no expressional change of Cx26 and Cx40 was detected by a high-dose flutamide. These results indicate that expression of Cx isoforms in the caudal epididymis is altered by exposure to steroidal compounds at the prepubertal age. It is suggested that a contact with environmental exogenous materials during the early postnatal period would lead to alteration of epididymal functions at the adult.

A proper development of the epididymis during the early postnatal development is required for successful fertility in the adult male. Direct cell-cell communication via connexin (Cx) molecules is a common way of cellular interactions to achieve normal development of a given tissue consisting of different cell types. The present research was attempted to determine the effect of exogenous exposure to estrogenic agonist or antiandrogen at the weaning age on expression of Cx isoforms in the adult corpus epididymis. Male rats were subcutaneously administrated with estradiol benzoate (EB) or flutamide (Flu) at the weaning age. The tissue was collected at 4 months of age. Expressional levels of Cx isoforms were determined by a quantitative real-time PCR. Statistical comparison showed significant increases of Cxs31, 32, 37, 40, and 43 transcript amounts by a treatment of 0.015 mg of EB /kg body weight (BW). A treatment of 1.5 μg of EB /kg BW caused a significant decrease of Cx43 gene expression but increases of Cxs26, 31, 32, 37, and 40 transcript levels. Exposure to 500 mg of Flu/kg BW induced an increase of Cx37 expression but significant decreases of Cxs43 and 45 mRNA levels. Expression of Cx37 was increased by a treatment of 5 mg of Flu/kg BW, while transcript levels of Cxs26, 30.3, 31, 31.1, 32, and 43 were significantly decreased by same treatment. These results demonstrate that exposure to steroidal compounds at the early developmental age alters expression of Cx isoforms in the adult corpus epididymis.