본 연구에서는 김치의 숙도를 조절하여 선도유지기간을 연장할 목적으로 천연항균소재로서 항균작용 및 항진균작용이 탁월한 식물성 천연항균소재(Botanical Antimicrobial Agent-Citrus fruits : BAAC)를 이용하여 김치의 변패에 관여하는 미생물들에 대한 생육억제효과를 관찰하였다. 김치의 산패에 관여하는 Lactobacillus plantarum, Klebsiella pneumonia, Pichia membranaefaci

다양한 기능성을 갖는 생약재 32종을 대상으로 천연보존료 개발의 일환으로 메탄올로 추출한 항균활성 물질을 몇 종의 병원균과 식중독균, 식품과 관련이 있는 세균, 젖산균 및 효모 등 12종의 균주 B. subtilis, M. luteus, Sta aureus, Sta. epidermidis, Strep. mutans, E. coli, Pse. aeruginosa, Sal. typhimurium, V. vulnificus, Lac. brevis, L

본 실험에서는 식품의 미생물작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(botanical antimicrobial agent)에 천연보조제(인삼추출물, 알로에, 매실추출물)를 첨가하여 paper disk법에 의한 항균성 검사를 실시한 결과, 인삼추출물을 첨가한 천연복합항균제재(BAAG)에서 생육저해환이 가장 크고 뚜렷하게 나타났다. 한편, 천연 항균복합제재 용액에 콩나물용 콩을 농도별로 각각 침지 처리하여 냉장 및 상온에 저장하면서

본 실험에서는 식품의 미생물작용에 의한 변질의 저해 효과가 뛰어난 식물성 천연항균제품(Botanical Antimicrobial Agent-Citrus product)에 홍삼추출물을 첨가하여 조제한 식물성 천연 항균복합소재(Botanical Antimicrobial Agent-Citrus and Red Ginseng extract mixture BAACG)의 항균력 검사, 열 및 pH안정성, 미생물 세포막의 형태 및 기능성 변화를 검토하고 BAA

The antibiotic and antioxidant effect of botanical antimicrobial agent-citrus product(BAAC) prepared from citrus fruits were investigated by using fishmeats made from pollack and ascidian fillet. We inoculated Salmonella typhi into fishmeats that were aferwards treated with BAAC varying the concentration level (50-500ppm) including the control not-treated with BAAC. While specimens were stored at 5 for designated period, we measured changes of total number of bacteria, peroxide level and textural properties. In addition, we performed organoleptic examination, focusing on appearance, texture and odor for fishmeats treated with BAAC and stored for designated period. For pollack and ascidian fishmeats, the initial total number of bacteria were 3.7l0CFU/g and 7.5l0CFU/g, respectively. After they were treated with 250 ppm of BAAC solution and stored for 5 days at 5, the figures reduced to 2.5l0CFU/g and 8.410CFU/g. No bacteria were found in fishmeats treated with above 500 ppm of BAAC, which remarkably proved antibiotic effect of BAAC solution. Furthermore, all the BAAC-treated specimens exhibited notable antioxidant effect. Fishmeats which were previously treated with 500 ppm of BAAC solution and stored for 30 days at 5, showed the lower peroxide level of 16.5 meq/kg than 68.9 meq/kg in the control of pollack and 21.2 meq/kg than 80.7 meq/kg in the control of ascidian. In the results of texture and organoleptic evaluation we could also observe good effects of BAAC on maintaining the freshness of fisher products.

본 실험에서는 가공 두부를 천연항균소재인 자몽종자추출 물의 유도체에 침지 처리한 후 꺼내어 상온에서 저장하면서 가공 두부의 품질(총균수, 수분 함량, 조지방의 함량, 조단백의 함량, 표면색도)변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 천연항균소재의 희석액에 침지처리한 가공두부는 대조구에 비하여 수분함량, 조단백 및 조지방 함량이 높게 나타났으며, 총균수는 천연항균소재 처리구의 증가폭이 무처리 대조구에 비해 감소하는 경향으로 나타

딸기를 항균소재에 침지 처리한 후 1% 농도로 항균성 소재를 첨가시킨 저밀도폴리에틸렌(LDPE) 필름에 의하여 딸기를 싸서 포장하고 5에 저장하면서 미생물 성장, 부패율, 텍스쳐, 화학적 품질을 측정한 결과 대조구에 비교해서 긍정적인 결과를 얻을 수 있었다. 즉, 항균성 소재를 포함시킨 LDPE 필름은 무첨가 대조 필름에 비해 호기성 총균수, 효모/곰팡이수로 측정된 미생물의 증식을 억제하는 것으로 나타났다. 이러한 미생물 증식억제의 효과로 인하여

The hydroxyapatite(HAp) as a carrier the for ion exchange agent of Ag+ions was prepared in semiconductor fabrication, The Ca/P molar ratio of the HAp was 1.65. The HAp is molded in shape of the antimcrobial ball and then sintered at 1000℃. Ag-containing HAp(HAp-Ag) was prepared by incorporating Ag+ions in HAp crystals through an ion-exchange reaction in solutions containing 0.01M AgNO3. The antimicrobial effect of HAp-Ag for bacteria such as Escherichia coli and Staphylococcus aureus has been investigated. The concentrations of silver in the antimicrobial ball was determined by inductively coupled plasma and the amount of Ag+ion was 9.0㎍/g. The HAp-Ag exhibited excellent antimicrobial effect for bacteria such as E. coli and S. aureus. The bactericidal activity was considered to be caused by direct contact of bacteria with Ag+ions in the HAp crystals. The HAp would likely to be possible as a carrier of antimicrobial metal ions such as Ag, Cu, and Zn by recycling of waste sludge in the semiconductor fabrication process.

항균력의 우수성이 확인된 천연항균소재인 대황추출물, 황련추출물 및 자몽종자추출물을 시설채소산물인 호박의 선도유지용 표면처리제 및 포장소재로 개발하여 저장실험을 실시한 견과는 다음과 같다. 호박을 항균소재에 침지처리한 후, 항균필름에 포장하고 1에서 저장하면서 저장호박의 경도, ascortbic acid함량, 중량손실을, 미생물 성장도 및 부패율을 측정한 결과, 대조구에 비교해서 긍정적인 결과를 얻을 수 있었다. 이는 대조구(LDPE) 필름에 비교

풋고추를 수확하여 선도를 유지할 수 있도록 하기 위하여 천연항균소재를 이용한 항균용제와 항균포장필름에 대한 각각의 효과와 두 가지를 병행한 상승효과를 확인하기 위하여 풋고추를 항균용제에 침지 처리후 1에 저장하면서 품질변화를 측정하였다. 그 결과 항균용제와 항균포장필름에 대한 각각의 효과뿐만 아니라 두 가지를 병행하여 저장하는 경우 상승효과가 더욱 두드러지게 나타나는 것을 확인 할 수 있었다. 따라서 풋고추의 선도를 유지하는 데에 천연항균소재가

The antimicrobial and glucosyltransferase(GTase) inhibition activity were searched for 30 species of various folk drugs and by products of food industry. Among them, two species, gallnut and red grape husk water extracts, were selected for the powerful antimicrobial and GTase inhibition activity. The polyphenol fractions of gallnut and red grape husk were showed very greater antimicrobial activity on both Gram(+) and (-), B. subtilis and E. coli. The minimum antimicrobial activity of gallnut polyphenol fraction were 1.0mg for B. subtilis and 3,0mg for E. coli. Red grape husk was 2.0mg for B. subtilis and 3.0mg for E coli. The polyphenol fractions of gall nut and red grape husk were showed powerful GTase inhibition activity. The concentrations of these fractions for 80% inhibition of GTase activity were 1.0810-3/mg/㎖ and 1.0810-2/mg/㎖, respectively. The most abundant compound in these fraction seems to be polyphenol derivatives. From these results, we think that the gallnut and the red grape husk polyphenol fraction had more antimicrobial and anti-plaque activities than artificial synthetic preservatives as an economic point of view.

The antimicrobial extracts of rheum palmatum and coptis chinensis root as well as grapefruit seed extract(GFSE) were applied to dipping treatment for keeping qualities of cucumbers, respectively, which were then packed in low density polyethylene(LDPE) films incorporated with 1 % antimicrobial extracts and stored . Dipping and packaging in the antimicrobial agents suppressed the growth of putrefactive microorgani는 and the decay ratio of cucumbers. In addition, the loss ratio of ascortic acid content and their weight was decreased during the sotrage of cucumbers. Consequently, the combined method of dippinf and packaging in antimicrobial agents turned out to be superior to dipping treatment or film-packaging in the view point of decay ratio and the keeping qualities of cucumbers.

To investigate the antimicrobial activities and effect of grapefruit seed extract(GFSE) on the physiological function of microorganism, antimicrobial activity, fatty acids of bacterial cell lipid and amino acids of bacterial cell protein were measured. The change of cell morphotype was observed by transmission electron microscope. GFSE was very stable on the wide range temperture and pH. The growth rate of E. coli and B. suvtilis were decreased above 40ppm GFSE There fore, minimum inhibitory concentration (MIC) of the E. coli and B. subtilis to GFSE were determined around 40ppm. In the change of fatty acids quantities, hexadecanoate was significantly decreased on the treatment compared with control in case of E. coli, whereas tridecanoate was not detected in case of B. subtilis. In the change of amino acids quantities, alanine, glutamic acid, glycine, lysine were decreased on the treatment compared with control in case of E. coli and B. subtilis Transmission electron microsgraphs(TEM) showed the microbial cells were destroyed by GFSE.

Various antimicrobial agents are widely used for the purpose of antimicrobial process. We investigated anlimicrobial activity and reduction efficiency of mal-ordour by the diphenyl ether compound (2,4,4` - trichloro - 2` - hydroxy diphenyl ether) against Staphylococcus aureus(S.aureus) and Proteus vulgaris(P.vulgaris) causing the mal-ordour. Especially, the diphenyl ether compound is not restricted to the regulation of water-contamination. In this research, we found that the optimum concentration of diphenyl ether compound was 1.5w% for both strains and antimicrobial expressions were c^0.38t = 2.56 for S.aureus, c^0.38t = 2.67 for P.vulgaris. We found also that -OH group played the role of antimicrobial functional group. Lastly, reduction effect of mal-ordour was more than 90% for both strain at the optimum conditions.