본 연구는 닭 진드기 구제를 목적으로 개발된 살비제인 와구잡이 II® (WGJB, 편백정유 : 계피정유 = 20 : 56)에 대하여 토끼와 기니픽을 이용하여 피부 자극성 및 감작성 평가를 각각 수행하였다. 일차피부자극시험에서 토끼의 피부에 WGJB를 24시간 동안 처리한 후 피부 자극성을 확인한 결과, WGJB는 비찰과 부위에서 홍반과 부종과 같은 어떠한 부작용도 일으키지 않았으나, 몇몇 토끼의 찰과 부위에서 매우 약한 홍반과 부종을 나타내어 WGJB의 1차 피부자극 지수는 0.625이었다. 따라서 WGJB는 약한 자극성이 있는 물질로 분류되었다. 피부감작성 시험에서 기니픽에 0.1 mL의 WGJB을 피내주사한 후 24시간 동안 감작시켰다. 감작 1주일 후 WGJB를 함유한 패취를 주사 부위에 부착하여 48시간 동안 처리한 다음, 2주 후에 WGJB 를 함유한 패취를 부착하여 감작을 야기시켰다. WGJB는 어떠한 알러지 반응도 나타내지 않았다. 따라서, 본 연구를 통하여 WGJB는 약한 피부 자극성을 가지며 감작성을 야기하지 않는 물질로 평가되었다.

The toxicity of cypress (Cupressus sempervirens) and howood (Cinnamomum camphora) essential oils, and its constituents to adult western flower thrips, Frankliniella occidentalis using leaf-dipping bioassay and vapour-phase toxicity bioassays. Both cypress and howood essential oils were toxic to western flower thrips. The most active principles were determined to be linalool, linalool oxide, cis-linalool oxide, trans-linalool oxide, camphor, and 1,8-cineole from cypress and howood essential oils. Linalool (24h LC50, 0.030 µg/cm2), linalool oxide (24h LC50, 0.036 µg/cm2), cis-linalool oxide (24h LC50, 0.043 µg/cm2), trans-linalool oxide (24h LC50, 0.045 µg/cm2), and camphor (24h LC50, 0.10 µg/cm2) were the most toxic. Potent toxicity was also observed with 1,8-cineole, a-pinene, d-limonene, a-terpinene, cinnamaldehyde, b-pinene, 3-carene a-terpineol, camphene, and terpineol (24h LC50, 0.33–0.65 µg/cm2). The spray bioassy of cypress and howood oil formulation (500 ppm) resulted in > 80% mortality toward western flower thrips population. Global efforts to reduce the level of toxic synthetic insecticides in the agricultural environment justify further studies on cypress and howood essential oil-derived materials as potential contact-action fumigants for the control of western flower thrips populations.

메치실린내성 황색포도상구균(MRSA)은 가장 중요한 지역 사회 병원성 균주의 하나로서 항생제 내성 문제가 세계적으로 대두되고 있다. 감염 치료에 대한 부작용과 내성이 적은 새로운 항생제의 개 발에 많은 관심과 연구가 필요하다. Cinnamomum camphora는 제 주도에서 자생하는 녹나무과의 식물이다. 민간에서는 신경 쇠약, 간질, 방광염, 신우 신염, 당뇨, 암과 당뇨병 치료제로 이용되고 있 다. 본 연구에서는 80% 에탄올을 이용하여 녹나무잎을 추출물하여 임상에서 분리한 메치실린 내성 균주에 대한 항균활성을 연구하였 다. 그 결과 녹나무잎 추출물에서 높은 항균활성을 관찰 할 수 있었 고, 항생제와의 병용처리 녹나무잎 추출물의 MIC와 MBC의 범위는 156-313 ㎍/㎖와 313-625 ㎍/㎖로 판독되었고, oxacillin의 병용 처리의 범위는 1288-256 ㎍/㎖와 128-512 ㎍/㎖, 그리고 ampicillin 의 병용처리 범위는 ㎍/㎖와 8-128 ㎍/㎖로 판독되었다. 녹나무잎 추출물과 항생제의 병용처리는 MRSA 항균활성에 대한 상승효과 가 높게 나타났다.

This study was carried out to investigate the effect of Cinnamomum camphora on melanogenesis. The MeOH extract of Cinnamomum camphora inhibited mushroom tyrosinase activity in dose-dependent manner. Moreover, it significantly suppressed the melanin production in melan-a cells at the concentration of 100μ/ml. The MeOH extract was partitioned with ethyl acetate, n-butanol and water. Among them, the BuOH soluble fraction exhibited significant inhibitory effect on mushroom tyrosinase. In addition, the BuOH soluble fraction reduced the melanin production in melan-a cells. But, the BuOH soluble fraction had less inhibition effects on melan-a cell originated tyrosinase. So, it was performed western blotting for melanogenic proteins (tyrosinase, tyrosinase-related protein (TRP-2)) using melan-a cells. The BuOH soluble fraction inhibited the protein expression of tyrosinase at the concentration of 100μ/ml. The results suggested that the BuOH soluble fraction of C. camphora might be a potent inhibitor of melanin biosynthesis in melan-a cells.

The volatile aromatic substances from Cinnamomum camphora and C. japonicum among warm-temperate tree species were examined for the physiological and psychological effects on the human body. Each scent of the trees was provided to the test materials. And then their brain wave, pulse rate, aroma sensibility evaluation and POMS were measured as follows. To examine psychological effect of aroma of each tree species on human bodies, aroma sensibility evaluation and POMS were conducted. The result reveals that the aroma of the two tree species were interpreted in terms of comfort, naturalness and stimuli, and that C. camphora shows low level of satisfaction due to strong aroma while C. japonicum is more preferred due to its soft and fragrant aroma. As well, POMS was given to identify subjects' temporary emotional state. The result indicates that C. camphora has higher values in every item than C. japonicum, and that both tree species repress anxiety and depression and create vivacious feelings with higher values in [vivacity] and [anger-hostility] items than [tense-anxiety] and [distress-depression]. To examine the physiological effect of aroma of each tree species, aroma extracted from the two tree species were distributed, and the subjects' brain wave and impulse were measured. The result demonstrates that C. camphor has relaxing effect of increasing α-wave and reducing pulse, and that C. japonicum also has relaxing effect, but the effect is less than that of C. camphora as it reduces pulse though it slightly reduces α-wave. In addition, individual preferences for C. camphora vary as it has big individual deviations vary, but C. japonicum has similar level of preference as its variances are small. The natural and pleasant aroma of these two tree species seem to have tranquilizing effect in both the physiological and psychological aspects and are found to have positive effects of feeling refreshed, feeling enhanced, and so on. In conclusion, there is a sufficient possibility of developing these two aromatic warm-temperate tree species. Also, the importance of such difference between these two tree species lies in the fact that they can set standards for the utilization of warm-temperate forest in the future.