Insects are the most diversified organisms in the world. They are vital to the functioning of the ecosystem and biosphere, and neither of these systems can operate effectively without insect interactions. Insects are regularly subjected to stressors related to increasing anthropogenic activities, including chemicals and climatic changes that induce major stresses. Depending on the species and the particular stress affecting it, insect populations respond by increasing or decreasing in numbers. Researchers have assessed the responses of insect populations to various direct and indirect environmental stresses. Some insect populations increase, while others decline. The reponses were determined by the particular environmental stress, the insect species, and he stage at which they were exposed to the stress.
Cotton aphid infests more than 700 plants and a major pest of various horticultural crops worldwide. The glucose regulated protein 78 (GRP78) is a member of heat shock protein 70. Its expression is associated with the nutritional changes as well as environmental stresses. The full sequences of grp78 cDNA of Aphis gossypii was determined. It had conserved motifs of hsp genes and terminated in KDEL which is common to GRP78. Quantitative realtime PCR showed that its level was changed during development and also upregulated by starvation. However, its level was not much changed by heat stress. The level of grp78 can be use to understand nutritional physiology on insects.
환경스트레스에 강한 내성을 지닌 신품종 톨페스큐를 개발할 목적으로 산화스트레스에 의해 강하게 유도되는 SWPA2 promoter 하류에 CuZnSOD와 APX 유전자가 엽록체에 동시에 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 가지며 재분화된 형질전환 식물체를 pot로 이식하여 기내 순화시킨 후, Southern 분석을 실시하여 본 결과, 발현벡터의 T-DNA
We previously demonstrated that root colonization of the rhizobacterium, Pseudomonas chlororaphis O6, induced expression of a galactinol synthase gene (CsGolS1), and resulting galactinol conferred induced systemic resistance (ISR) against fungal and bacterial pathogens in cucumber leaves. To examine the role of galactinol on ISR, drought or high salt stress, we obtained T-DNA insertion Arabidopsis mutants at the AtGolS1 gene, an ortholog of the CsGolS1 gene. The T-DNA insertion mutant compromised resistance induced by the O6 colonization against Erwinia carotovora. Pharmaceutical application of 0.5 - 5 mM galactinol on roots was sufficient to elicit ISR in wild-type Arabidopsis against infection with E. carotovora. The involvement of jasmonic acid (JA) signaling on the ISR was validated to detect increased expression of the indicator gene PDF1.2. The T-DNA insertion mutant also compromised tolerance by increasing galactinol content in the O6-colonized plant against drought or high salt stresses. Taken together, our results indicate that primed expression of the galactinol synthase gene AtGolS1in the O6-colonized plants can play a critical role in the ISR against infection with E. carotovora, and in the tolerance to drought or high salt stresses.
1. 본 연구에서는 완두유래의 세포질성 PsAPX1 유전자를 대상으로 산화스트레스 유도성 프로모터를 연결하여 엽록체에 targeting 되는 과발현 운반체를 제작하고 벼에 도입한 결과 형질전환체에서 도입유전자 수가 1~3 copy인 것으로 나타나, 적은 수의 유전자가 안정적으로 도입되었음을 확인하였다. 2. 염, 오존, 자외선, 한발과 같은 다양한 환경스트레스 조건에서 내성이 증진된 우수 계통을 선발하기 위하여 작성된 형질전환 벼 계통들을 대상으로 생물
"Chugang" as a new double cropping potato variety for table use was bred in 2003 from a cross between H83005-2 with short dormancy and "Superior" with common scab resistance. It had been evaluated for short dormancy, growth adaptability and tuber characteristics every twice a year from 1990 to 1999. Regional yield trials had been performed from 2000 to 2002 at three locations, Jeju, Namhae and Gangneung of Korea, respectively. It has round oblong tuber shape with yellow skin and white flesh color. Its tuber dormant periods is approximately 50~60 days. It showed less physiological disorders such as cracking or knobs, and more tolerant to environmental stresses such as plant death by flooding and damages by frost or severe wind compared to "Dejima". Average yields of "Chugang" are 34.6 and 31.1 ton/ha at 90 days after planting in spring and autumn cropping, respectively. It is more resistant to common scab and less susceptible to late blight (phythophthora infestans) compared to "Dejima". However, it is susceptible to potato virus Y (PVY) and andean mosaic virus (AMV).
A six-rowed naked barley cultivar, Donghanchal (Hordeum vulgare L.), with low-amylose, winter hardiness, viral disease resistance and good quality, was developed from the cross between the advanced line “Masangwamac/Kangbori*7” and Naehanssalbori in 1993.
This study was conducted to classify photosynthesis rate and changes of rutin content of Korean buckwheat (cv. Chunchon-jaerae and Yangjeul-memil) treated with salinity, UV-C and low temperature. In case of cv. Chunchon-jaerae and Yangjeul-memil, according to the salt stress, transpiration rate, stomatal conductance and photosynthesis rate were decreased. Both cultivars also showed decrease of transpiration rate and photosynthesis rate under the UV-C. Rutin contents within leaf and stem of cv. Yangjeul-memil were decreased when NaCl concentration was high. Rutin contents within leaf and stem of cv. Yangjeul-memil were generally decreased when the time laps under the UV-C stress. Rutin contents within leaf and stem of cv. Chunchon-jaerae was also generally decreased when the time laps under the low temperature stress.
Environmental stresses during culture of food legumes can be classified to the three categories; the climatic stresses, pests, and public pollution. Of the researches on the climatic stresses in Korea, drought injury and lodging injury were studied more comparing with excess water injury, cooling injury, wind injury, frost injury and hail injury. Although many researches have been done on the pests, mainly on the control of pests, a few researches have been done on the basic research. Recently public pollution is considered one of major stresses during culture of crops following rapid development of industry in Korea. A few researches have been done on the air pollution and acid rain. Therefore many researches should do on the environmental stresses during culture of food legumes in the future.
The primary countermeasure for environmental stresses of crops grown in Korea would be to maintain top soil content of available B for upland crops and Si for low land rice in balance with other nutrient elements such as N, P and K, so as to maintain those nutrient balances in plants. Development of standard levels of elements in soils for balances uptake of those elements by plants are needed under the multi nutrient factor balance concept using the soil test results.