미국흰불나방(Hyphantria cunea Drury)의 방제약제로 등록되어 있는 19종의 약제를 사용하여 미국흰불나방 노숙유충에 대한 방제효과를 조사하였다. 그 결과 pyrethroid계인 deltamethrin EC, etofenprox EC, lambda-cyhalothin EC, esfenvalerate EC와 emamectin benzoate EC, tebufenozide WP, chlorantraniliprole WG, pyridalyl EW은 약제 살포 후 48시간 조사에서 90% 이상의 높은 방제효과를 보였다. metaflumizone EC는 72시간 경과후에 96.7%의 살충효과를 보였으며, methoxyfenozide SC는 96시간 경과 시점에서 100%의 높은 방제효과 가 나타내었다.

In this study, an simultaneous LC-MS/MS multi-residue analytical method was developed and validated for the residues of six neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) in honey. Sample preparation included a combination of QuEChERS extraction kit and liquid-liquid extraction method to effectively extract pesticide components from the honey matrix and optimized analytical conditions to achieve high sensitivity and selectivity. The limits of detection (LOD) and the limits of quantitation (LOQ) were set in the range of 6-15 ng/mL and 19-44 ng/mL, respectively and the correlation coefficient (R²) was greater than 0.99, confirming good linearity. In addition, the intra-day recoveries for each pesticide were 75-104%, and the coefficient of variation (CV) was less than 20%, which met the guideline recommended by the Ministry of Food and Drug Safety. The LC-MS/MS method developed in this study is expected to be used as a multi-residue analysis method for 6 neonicotinoid pesticides in honey.