The MTT assay is one of superior evaluation methods widely used to analyze the viability of metabolically active cell. It can be used to determine the percentage of viable sperm through measurement of the reduction of MTT granules at mitochondria in sperm tail. The purpose of this study is to determine the optimal condition of a simple and easy MTT assay to validate boar sperm viability and compare the accuracy of this test with microscopic examination. The MTT reduction rate for sperm viability were analyzed in microtiter plates (96 well) from 1 hr to 5 hr incubation periods at using spectrophotometer (microplate reader) at 550 nm wavelength. The remainder of semen sample was simultaneously examined to compare the correlation of accuracy between MTT assay and other sperm parameters. Those sperm parameters were included the motility, survival rates, membrane integrity, mitochondria activity and acrosome integrity. The OD values of MTT assay (MTT reduction rates) did not greatly change at 1 hr to 5 hr incubation periods in different proportion of live and freeze-killed sperms (dead sperm). The MTT reduction rates or survival rates were decreased according to the different concentration of live and dead sperm. The linear regression at 1 hr and 4 hr incubation periods in sperm MTT assay was y=291.55x-72.176 and y= 180.64x-44.569, respectively. There are high correlation between 1 hr and 4 hr incubation periods (p<0.001). The results of MTT assay and other sperm parameters has a positive correlation (p<0.01 or 0.05). The correlation coefficients for MTT assay was 0.88115 for motility, 0.89868 for survival rates, 0.91722 for membrane integrity and 0.77372 for acrosome integrity, respectively. In conclusion, the MTT assay can be used as a reliable and efficient evaluation method for boar sperm viability. It can be use practical means to evaluate the quality of boar sperm by a fast, inexpensive and easy method.

삼차원적 골격구조를 가지며 세공의 크기와 산성도가 유사한 SAPO-44, SAPO-34(CHA type), SAPO-17(ERI type), 그리고 1차원적 고격구조를 가진 ZSM-23(MTT type) 촉매재료를 합성하고, 메탄올 전환방응에 있어서의 촉매적 성능을 비교 검토하였다. 이들의 산성도 세기는 SAPO-44>SAPO-34>SAPO-17>ZSM-23 순으로 나타났다. 메탄올 전환반응을 비교한 결과, 삼차원적 골격구조를 가지고 있는 SAPO-34와 SAPO-44에서 높은 에티렌 선택성이 얻어졌으며, 특히 Ni을 골격내에 도입시켰을 때 그 선택율은 더욱 증가하였다. 반면에 1차원적 골격구조를 가지고 있는 ZSM-23에서는 입체적 형상선택성의 감소로 올레핀보다는 파라핀의 선택율이 우세하였다.

자광찰벼, 화선찰벼, 및 일품벼 에탄올 분획의 H4IIE 세포에 대찬 산화적 스트레스 보호효과를 검정하기 위해서 수행하였다. 과산화수소에 의하여 생성된 자유라디칼은 H4IIE 세포에 산화적 손상을 초래하며 이러한 손상에 기인된 세포사멸에 대하여 화선찰벼와 일품벼 추출물은 방어효과가 없었으나, 적색을 띤 자광찰벼 추출물의 에탄올 분획은 과산화수소에 의하여 유도된 산화적 스트레스에 가장 현저한 효과를 나타내었다. 금후, 보다 광범위하고 집중적인 연구를 수행한다면 쌀 함유 유효성분들의 생리활성에 관한 보다 명확한 효과가 구명될 수 있을 것으로 사료된다.