This study applied a camera trapping method to investigate species diversity of birds and mammals in Jingwan-dong Wetland located in Bukhansan National Park, Seoul, Korea. The objectives of this study were to (1) verify the efficiency of the camera trapping method through a combination of literature and observation surveys, and to (2) propose it as an effective monitoring method to assessing changes in biodiversity. From February 2022 to June 2022, a total of six cameras were installed for 121 days to conduct camera trapping in three aquatic environments. As a result, a total of 14,742 videos were obtained with a data acquisition rate of 59.2%. Analysis of the data identified a total of 20 families and 47 species of birds with 7 families and 8 species of mammals. When previous field observation data compiled from the past 10 years starting from 2011 were analyzed, a total of 33 families and 90 species of birds with 5 families and 6 species of mammals were identified. Camera trapping in Jingwan-dong Wetland recorded species list, including 3 families and 3 species of bird and 2 families and 2 species of mammal not observed in the past decade. Thus, camera trapping, which complements temporal limitations of field survey, can be an effective monitoring method for rapidly changing biodiversity if spatial limitations are improved. Resulting species lists can serve as a basis for future restoration and management plans.

In a conventional sense, dried-spermatozoa are all dead and motionless due to the lost of their natural ability to penetrate oocytes both in vivo and in vitro. However, their nuclei are completely able to contribute to normal embryonic development even after long-term preservation in a dried state when the dried-spermatozoa are microinjected into the oocytes. In this sense, dried spermatozoa must still be alive. Thus, defining spermatozoa as alive or dead seems rather arbitrary. Several drying method of sperm including freeze-drying, evaporative/convective-drying and heat-drying were represented in this review. Although the drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genetic resources.

림프구는 면역기능의 중심적인 역할을 담당하는 세포이고, 운동에 의해 변화하고 노화에 의해 기능이 저하하는 것으로 잘 알려져 있지만, 고령자를 대상으로 운동에 의한 림프구의 변동에 대한 연구는 지금까지 미흡한 실정이다. 그래서 본 연구에서는 운동훈련이 고령흰쥐의 림프구에 미치는 영향에 대해 조사하였다. 8주간의 운동처리가 비장세포 중 그리고 장간막 림프절의 림프구에 미치는 영향을 분석했다. 비장세포중의 림프구에 대해서는 헬프-T 림프구, 세포상해성 T 림프구, 그리고 보조자극 신호 수용체 발현 T 림프구의 비율이 운동처리구에 있어 유의적으로 높은 수치를 나타냈다. 한편, 비장세포중의 B 림프구 및 장간막 림프절중의 림프구에 대해서는 두 처리구 간에는 유의적인 차이는 보이지 않았다. 이상의 결과들로부터, 운동처리구가 흰쥐 비장세포중의 T 림프구수에 영향을 미치는 것이 밝혀졌다. 그러나 면역기능을 평가하기 위해서는 림프구의 수 뿐만 아니라 기능에 대해서도 면밀히 검토해야 한다. 금후에는 운동처리구가 림프구의 기능에 미치는 영향을 해석할 필요성이 사료된다.

본 연구는 경상북도 청도군 운문산 자연휴식년제 지역일대의 포유류상을 밝히기 위해 2007년 12월부터 2009년 9월까지 수행되었다. 조사결과 6목 11과 24종의 포유류의 서식을 확인하였다. 우점종으로는 고라니 Hydropotes inermis, 다람쥐 Tamias sibiricus, 두더지 Mogera wogura, 흰넓적다리붉은쥐 Apodemus peninsulae 등이었다. 특히, 천연기념물(NM)과 환경부지정 멸종위기종(ES)인 하늘다람쥐(Pteromys volans; NM #328, ES II급), 수달(Lutra lutra; NM #330, ES I급), 삵 (Prionailurus bengalensis; ES II급), 담비 (Martes flavigula; ES II급) 등 법적보호종 4종이 발견되었다. 다양한 포유류 종과 법적보호종의 서식에 대한 지리적 분포를 확인한 이 결과는 조사지역이 포유류의 서식에 필요한 자연생태계의 매우 양호한 조건을 갖추고 있는 지역임을 반영한다. 반면, 관리동물 종으로 지정된 들고양이가 다수 관찰되었다. 운문산 자연 생태계의 건전한 유지와 생태계 위해 동물의 관리 방안을 마련하기 위해서는 지속적이고 장기적인 연구조사가 필요하다.

Vascular endothelial growth factor (VEGF) was a main substance as an endothelial cell specific mitogen. This acts sometimes as an antiapoptotic factor. It plays an important role in the embryo development as well as implantation process. This review introducesthe function and distribution of the VEGF and its receptors related to the development of mammal embryos.

This experiment was carried out to study the determination of survival of vitrified and thawed mammal follicular oocytes by FDA-test. Oocytes were divided into 3 groups according to attachment of cumulus cell. Group A oocytes were tightly surrounded by cumulus cell, group B oocytes were partially surrounded by cumulus cell, and group C oocytes were poorly surrounded by cumulus cell. Vitrification solution developed by our previous study (Kim et al, 1992) which consisted of permeable agent (20 % glycerol + 10 % ethylene glycol) and nonpermeable agent (30 % Ficoll + 10 % sucrose). Oocytes (7~10) loaded into 0.25 ml straw after 10 min equilibration were plunged into liquid nitrogen (- 196) directly. The FDA-score of vitrified and thawed group A oocytes was higher in rat (4.2) than in rabbit (3.9), cow (3.8), mouse (3.4) and porcine (2.4), however that of cumulus cell was higher in rabbit (4.7) than in rat (4.1), cow (2.9), porcine (2.6) and mouse (1.4). The FDA-score of vitrified and thawed group B oocytes were 3.1 (cow), 2.9 (rabbit), 2.9 (mouse), 2.6 (rat) and 2.5 (porcine), respectively. However that of cumulus cell was higher in rabbit (3.7) than in porcine (2.6), rat (2.3), cow (1.7) and mouse (0.3). The FDA-score of vitrified and thawed group C oocytes was higher in mouse (4.1) than in cow (2.9), rabbit (2.6), rat (1.3) and porcine (1.1). As shown in the above results, The survival rates of oocytes were higher in group A than in group B and C except in mouse and cow. These results suggest that the survival of cumulus cell as well as follicular oocytes can be reliably judged by their fluorescence with FDA-test.