The radiological characterization of SSCs (Structure, Systems and Components) plays one of the most important role for the decommissioning of KORI Unit-1 during the preparation periods. Generally, a regulatory body and laws relating to the decommissioning focus on the separation and appropriate disposal or storage of radiological waste including ILW (intermediate level waste), LLW (low level waste), VLLW (very low level waste) and CW (clearance waste), aligned with their contamination characteristics. The result of the preliminary radiological characterization of KORI Unit-1 indicated that, apart from neutron activated the RV (reactor vessel), RVI (reactor vessel internals), and BS (biological shielding concrete), the majorities of contamination were sorted to be less than LLW. Radiological contamination can be evaluated into two methods. Due to the difficulties of directly measuring contamination on the interior surfaces of the pipe, called CRUD, the assessment was implemented by modeling method, that is measuring contamination on the exterior surfaces of the pipes and calculating relative factors such as thickness and size. This indirect method may be affected by the surrounding radiation distribution, and only a few gamma nuclides can be measured. Therefore, it has limitation in terms of providing detailed nuclide information. Especially, α and β nuclides can only be estimated roughly by scaling factors, comparing their relative ratios with the existing gamma results. To overcome the limitation of indirect measurement, a destructive sampling method has been employed to assess the contamination of the systems and component. Samples are physically taken some parts of the systems or components and subsequently analyzed in the laboratory to evaluate detailed nuclides and total contamination. For the characterization of KORI Unit-1, we conducted the radiation measurement on the exterior surfaces of components using portable instruments (Eberline E-600 SPA3, Thermo G20-10, Thermo G10, Thermo FH40TG) at BR (boron recycle system) and SP (containment spray system) in primary system. Based on these results, the ProUCL program was employed to determine the destructive sample collection quantities based on statistical approach. The total of 5 and 8 destructive sample quantities were decided by program and successfully collected from the BR and SP systems, respectively. Samples were moved to laboratory and analyzed for the detail nuclide characteristics. The outcomes of this study are expected to serve as valuable information for estimating the types and quantities of radiological waste generated by decommissioning of KORI Unit-1.

The PoN (Proof of Nonce) distributed consensus algorithm basically uses a non-competitive consensus method that can guarantee an equal opportunity for all nodes to participate in the block generation process, and this method was expected to resolve the first trilemma of the blockchain, called the decentralization problem. However, the decentralization performance of the PoN distributed consensus algorithm can be greatly affected by the network transaction transmission delay characteristics of the nodes composing the block chain system. In particular, in the consensus process, differences in network node performance may significantly affect the composition of the congress and committee on a first-come, first-served basis. Therefore, in this paper, we presented a problem by analyzing the decentralization performance of the PoN distributed consensus algorithm, and suggested a fairness control algorithm using a learning-based probabilistic acceptance rule to improve it. In addition, we verified the superiority of the proposed algorithm by conducting a numerical experiment, while considering the block chain systems composed of various heterogeneous characteristic systems with different network transmission delay.

This study was investigated to test whether the zygote recognized the topoisomerase II beta (TOP2B) mediated DNA fragmentation in epididymal spermatozoa or the nuclease degradation in vas deferens spermatozoa by testing for the presence of gammaH2AX (γH2AX). The γH2AX is phosphorylation of histone protein H2AX on serine 139 occurs at sites flanking DNA double-stranded breaks (DSBs). The presence of γH2AX in the pronuclei of mouse zygotes which were injected with DNA broke epididymal spermatozoa was tested by immunohistochemistry at 5 and 9 h post fertilization, respectively. Paternal pronuclei that arose from epididymal spermatozoa treated with divalent cations did not stain for γH2AX at 5 h. On the other hand, in embryos injected with vas deferences spermatozoa that had been treated with divalent cations, γH2AX was only present in paternal pronuclei, and not the maternal pronuclei at 5 h. Interestingly, both pronuclei stained positively for γH2AX for all treatments and controls at 9 h after sperm injection. In conclusion, the embryos recognize DNA that is damaged by nuclease, but not by TOP2B because H2AX in phosphorylated in paternal pronuclei resulting from spermatozoa treated with fragmented DNA from vas deferens spermatozoa treated with divalent cations, but not from epididymal spermatozoa treated the same way.

In order to investigate novel insect growth regulators (IGRs) from actinomycetes, 363 isolates of actinomycetes were tested for their IGR activities. Among them, Streptomyces sp. AN120537 showed the highest juvenile hormone antagonist (JHAN) activity and significant insecticidal activities against larvae of Aedes albopictus and Plutella xylostella. In addition, the dead larvae showed morphological deformities such as contraction of body segments or pigmentation of all body parts. Through liquid chromatography and bioassay-guided fractionation, 7 IGR compounds were identified from the crude hexane extract of AN120537. These results suggested that Streptomyces sp. AN120537 could be useful resources for development of eco-friendly insecticidal agents.

ABSTRACT The growth of the social platform has led to a rapid increase in sustainable value cocreation activities with customers in order to know what a customer wants. In this study, we have studied about co-creation experience in social platform service. Previous prior studies have systematically classify co-creation between customer and enterprise as co-product and value-in-use, Conceptual factors. The co-creation and value-in-use factors theoretically derived from previous researches attempt to investigate the relationship between value co-creation experience and online service quality. We also tried to investigate the relationship between value co-creation experience and online service quality, customer equity, and online word of mouth. To do this, social platform services are divided into open sns and closed sns. Open SNS was targeted to Facebook, Twitter, and Pintrest users. Closed sns were targeted to Instagram, Cyworld, Band, and KakaoTalk users. In this study, it was confirmed that the relationship between online service quality, customer equity, and online word of mouth is significant through value co - creation experience. In the future, companies that design social platforms will be asked to do what they should do to co-create with customers, and provide suggestions for how to operate the platform in order to continuously activate value co-creative experiences.

In a conventional sense, dried-spermatozoa are all dead and motionless due to the lost of their natural ability to penetrate oocytes both in vivo and in vitro. However, their nuclei are completely able to contribute to normal embryonic development even after long-term preservation in a dried state when the dried-spermatozoa are microinjected into the oocytes. In this sense, dried spermatozoa must still be alive. Thus, defining spermatozoa as alive or dead seems rather arbitrary. Several drying method of sperm including freeze-drying, evaporative/convective-drying and heat-drying were represented in this review. Although the drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genetic resources.

Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, H+-transporting ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.