이 연구를 통해 우리나라에서 복원 중인 멸종위기종인 반달가슴곰(Ursus thibetanus ussuricus)의 식별을 위한 새로운 분자 marker 체계를 개발하였다. 전장 유전체 서열 정보를 사용하여, 20개의 새로운 미세부수체(Microsatellite, MS) marker가 다중 중합효소연쇄반응(PCR)을 기반으로 하는 유전자형 분석을 통해 개발되었다. 개발된 MS marker 20종의 대립유전자 수는 3개에서 10개였으며, 평균 대립유전자 수는 6.05개였다. 관측 이형접합도(Hobs), 기대 이형접합도(Hexp), 다형정보량(PIC)의 평균은 각각 0.683, 0.715, 0.658로 나타났다. Fis 값은 –0.385에서 0.438의 범위에 있었고, 평균은 0.052였다. 무작위 교배(PIrandom), 반형매 교배(PIhalf-sib), 전형매 교배(PIfull-sib)에서의 동일개체출현율은 각각 1.58×10-18, 2.79×10-14, 4.75×10-8를 나타내었다. 이 연구의 결과는 새로운 MS marker 체계가 높은 유전적 다양성과 낮은 PI 수치를 가지고 있어, 야생에서 출현하고 있는 반달가슴곰들을 식별하거나 새끼 곰의 부모 추적에 유용하게 사용될 것으로 생각된다. 이 분자 marker 체계는 한국에서 복원 중인 반달가슴곰 개체군의 관리와 유전적 다양성 증진에 기여할 수 있을 것으로 사료된다.

Background: With the growing interest in the health of companion dogs, their average lifespan has increased, leading to an increase in the proportion of elderly dogs. As elderly dogs are vulnerable to various diseases, there is a need for alternatives to predict the risk of major diseases in senior dogs, prevent them in advance, and manage their health effectively. Therefore, this study was conducted to identify candidate genes and single nucleotide polymorphisms (SNPs) influencing primary angle-closure glaucoma, a major disease in elderly dogs, using the Axiom Canine HD Array and establishing foundational data. Methods: Samples from 95 dogs of 26 breeds from South Korea were analyzed using an SNP chip. Ultimately, two SNPs were selected. To assess the impact of non-synonymous SNP (nsSNPs), functional analysis of candidate genes, Hazard Assessment, and protein structure prediction were conducted. Sequencing for SNP validation involved samples from 95 dogs of ten breeds with reported domestic and international glaucoma cases. Results: The candidate gene TNS1 was associated with the integrin signaling pathway. The selected nsSNP was found to cause a mutation at the ninth position of the amino acid sequence, changing serine to leucine and resulting in alterations to the overall protein structure. Sequencing analysis results for SNP validation revealed differences in frequency among breeds. Conclusions: The identified SNP markers are potential risk prediction tools. Utilizing genotype frequency data by breed and individual could aid in disease management and contribute to advancements in the medical industry.

Tricholoma matsutake is a commercially valuable fungus that produces gourmet mushrooms. The artificial cultivation method that is currently used to produce fruiting bodies involves the transplantation of pine trees infected with T. matsutake from their natural habitats and the successful infection of young pine trees and mushroom production have been reported in Hongcheon. In this study, T. matsutake genetic diversity and relationships in this region were investigated. A genotype analysis was conducted on 25 fruiting bodies collected from an area approximately 2.56 km2 in size using 10 simple sequence repeat (SSR) markers. The analysis revealed 25 genotypes and 23 alleles with mean observed (HO) and expected (HE) heterozygosities of 0.312 and 0.293, respectively. The average polymorphism information content (PIC), which indicates marker polymorphism, was 0.257. A phylogenetic analysis showed no clear correlation between collection location and genetic distance; a spatial autocorrelation analysis indicated no significant genetic structure within 1600 m; and the Mantel test showed no significant correlation between genetic and geographic distances. These results were consistent with previous reports on Japanese populations. This study provides insights into the genetic diversity and structure of T. matsutake at a local scale and highlights the need for further studies across broader geographical ranges.

기동상은 미완료상으로서 문장 차원에서 동작이나 상태 변화가 곧 시작하는 의 미뿐만 아니라 진행하는 의미도 있다. 동작이나 상태 변화가 시간 축에서 한 동안 에 진행해야 되기 때문이다. 기동상 표지는 시작 의미만 나타내는 ‘起来’외에 시 작하고 진행하는 의미를 표시하는 표지도 포함되어야 한다. 중국어 기동상 표지 라고 하면 대부분 방향보어인 ‘起来’를 떠올리기 시작한다. 그러지만 통사적으로 분석해 볼 때는 기동상 표지는 더 있는 것이다. 다른 방향보어인 ‘上来’, ‘下去’, ‘下来’가 상태 동사와 결합하여 선행 상태 변화가 곧 시작하거나 시작한 다음에 지속/진행하기는 의미도 있으므로 기동상 표지로 간주해야 된다. 보어‘起’, 상태 동사, 인지 동사, 조동사 ‘要’, ‘该’, ‘想’은 완료상 표지 ‘了’와 결합하여 ‘기동’ 의 미를 표시할 수도 있다. 다만 이 표지들이 ‘起来’와 달리 구조적, 의미적 제약을 고려해야 된다. 결과적으로 보어 ‘下来’는 미연(未然)사건과 결합하여야 되며 ‘下 去’는 이연(已然)사건과 결합하여야 된다. 그리고 상태 동사가 완료상 표지 ‘了’와 공기하여야 된다. 이외에는 당위성 조동사 ‘要’, ‘该’, ‘想’와 ‘S了’의 결합은 반사 실적 상황으로서 동작이 곧 나타나는 의미이니까 기동상 표지로 간주할 수 있다.

장미 속은 오랜 시간에 걸쳐 잡종화, 배수화 및 육종 등을 통해 약 250개의 종과 30,000여 품종이 존재하는 진화 역사를 가지고 있으며, 다양한 분류체계로 구분되고 있다. 이에, 본 연구 는 장미 속 자원의 다양성을 평가하고 자생식물 해당화의 육종적 활용을 위해 수행되었다. 장미 속 자원의 형태학적 다양성을 확인하기 위해 장미 속 자원 303점에 대해 형태적 특성조사를 실시하고 해당화 및 장미 속 자원 29점을 선발하여 SSR 분석을 실시하였다. 추가적으로, 해당화의 육종적 활용을 위한 자원 6점 을 선발하고 화분 검정을 실시하였다. 본 연구의 결과, 장미 속 자원 303점의 다양성 평가 결과에서 유사한 형태적 특징을 지닌 자원끼리 7개 그룹으로 나뉘었다. 선발된 29개 자원의 형태학적 데이터와 분자학적 데이터를 이용한 군집 분석 결과, 데이터의 유사성을 보인 자원끼리 각각 5개, 4개 그룹으로 구분되었다. 또한, 혼합분석 시에는 3개 그룹으로 확인되었다. 분류 결과를 바탕으로 자생 식물을 이용한 정원장미 육종을 위해 각각 특성이 다른 해당화를 3점 선발하였고 장미 속 자원에 속하는 정원 장미 3점을 선발하여 총 6자원을 선발하였다. 선발된 자원의 화분 검정 결과, 종간 교잡체를 제외한 5가지 자원에서 90% 이상의 정상화분율을 확인하였다. 본 결과는 자생식물 해당화를 재평가 하고 정원 장미 육종 전략 수립에 도움이 될 것으로 기대된다.

참진드기과(Ixodidae) 속하는 개피참진드기(Haemaphysalis flava)는 동남아시아에서 남아시아에 걸쳐 분포 하며, 다양한 질병을 매개하는 것으로 알려져 있다. 특히 중국, 일본, 한국에서 개피참진드기의 주요 매개 질병인 중증열성혈소판감소증후군(SFTS)의 감염 사례가 지속적으로 증가하는 것으로 보고되고 있다. 이 연구는 Illumina HiSeq 4000 시퀀싱을 통해 raw 데이터를 획득하고, Trinity를 기반으로 de novo assembly를 수행하여 unigene을 확보하였다. 이 결과, 총 69,822개의 unigene이 생성되었으며, 이 중 46,175개의 unigene이 PANM-DB에 annotation 되었다. 또한 KOG, GO 및 KEGG 분석을 통해 30,000개, 19,074개, 9,333개의 unigene이 annotation 되었 으며, InterProScan 결과를 통해 protein kinase, zinc finger (C2H2-type), reverse transcriptase, RNA recognition motif domain 등과 같은 세포 조절 메커니즘과 관련된 유전자가 확인되었다. RepeatMasker(v4.0.6)와 MISA(v1.0)를 사 용하여 unigene에서 SSR 마커를 확인한 결과, 총 3,480개의 SSR 마커가 확인되었으며 이 중 trinucleotide 반복이 1,907개, dinucleotide 반복이 1,274개로 확인되었다. 이러한 연구 결과는 H. flava 암컷의 유전자 및 유전자 조절 메커니즘을 이해하는데 기초 자료로서 활용 가능하며, 질병 전파 감수성에 대한 후속 연구에 유용한 정보를 제공 할 것으로 사료된다.

Stoneflies (Plecoptera) are known for being sensitive to water pollution and are used as bioindicators for evaluating water quality. Among them, Nemouridae, especially the genus Nemoura, which are commonly referred to as winter stoneflies, can be found around streams even during the cold winter months. Nemoura geei Wu, 1929, among them, was originally described from Beijing and is widely distributed in Korea, China, Japan, and the Russian Far East. Here, we report the development and characterization of new functional microsatellite markers of N. geei using high-throughput sequencing technology. A total of 80,661 microsatellite loci were identified with a total length of 1,801,591 bp. The average length was 22.34 bp, and microsatellites occupied 0.42% of the entire sequence. The novel 20 microsatellite markers developed in this study can be usefully applied to the population genetics analyses as important genetic resources for understanding the ecological and evolutionary characteristics of a stonefly species at the population level in Korea.

This study focuses on developing diagnostic compositions, kits, and information provision methods for identifying species-specific genes in domestically residing Reticulitermes speratus and Reticulitermes kanmonensis, as well as the recently introduced Cryptotermes domesticus. The core innovation of this invention lies in the utilization of species-specific genetic markers to facilitate rapid and accurate species identification using a PCR (polymerase chain reaction)-based diagnostic technique. This approach enables swift identification of termites at quarantine stages, contributing to efficient management of imported goods and minimizing ecological and economic damages caused by termites. Through genome analysis of termites, this research has identified candidate species-specific genetic markers, developed diagnostic compositions and kits based on these markers, and proposed a rapid diagnostic method capable of determining termite species within a day, optimally within three hours. This invention provides a groundbreaking tool for termite management and research, significantly contributing to pest control and biodiversity conservation efforts.

The oriental fruit fly, Bactrocera dorsalis, is highly polyphagous pest that damages over 400 plant species, including commercially grown crops and fruits. It was first described in Taiwan and has since invaded Japan and China. In 2021, B. dorsalis was reported on Jeju Island in the Republic of Korea (ROK). To investigate its origin and understand its colonization pathway in ROK, the genetic characteristics were analyzed by using mitochondrial genes. The study analyzed the haplotypes and genetic differences among populations oriental fruit flies collected from Southeast Aisa, including Thailand, Taiwan, and Vietnam. The mitochondrial genes cytochrome oxidase subunit I (COI) and cytochrome B (CytB) were used for the analysis. The results showed that B. dorsalis had high levels of haplotype diversity among species. No major haplotype was found among populations. The present study enhances our understanding of the haplotype diversity of B. dorsalis in neighboring countries of ROK. This will expand our knowledge of the source of origin and invasive pathway for B. dorsalis.

The pear pest, Cacopsylla jukyungi (Hemiptera: Psyllidae), is one of the most damaging insect to commercial pears in South Korea. In this study, we developed eight microsatellite markers specific to C. jukyungi and genotyped 132 individuals collected from 11 localities throughout South Korea. Populations showed lower observed heterozygosity than expected heterozygosity and slightly or highly positive values of inbreeding coefficients, suggesting that C. jukyungi is subjected to inbreeding. The nationwide expansion of pear orchards and the replacement with a popular new cultivar during the last 50 years, which may have accompanied the spread of C. jukyungi-bearing pear grafts and scions, are likely sources of such facilitated dispersal. Thus, a management strategy against unintended anthropogenic dispersal of the pear psyllid will be required for better control of C. jukyungi.

In Korea, both Sympetrum depressiusculum Sélys, 1841 (Odonata: Libellulidae) and Sympetrum frequens Sélys, 1883 are recorded. However, the identity of Korean populations and the validity of listing the two species have not yet been settled. In this study, we collected 74 individuals from Kroea, Russia, the Netherlands, and Japan. These were sequenced for COI, 16S rRNA, and ITS region. Major morphological characters and phylogenetic, network, and structure analyses all consistently suggest that Korean populations form a single species. Consequently, it could be valid to treat Korean populations as one species, S. depressiusculum, by applying the senior name.

Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.

Cucurbita is one of the crops with high demand in the world. Securing breeding sources is crucial and fundamental to any plant breeding program. This study was conducted to investigate the characterization of phenotypic traits and phylogenetic classification of germplasm provided by the National Agrobiodiversity Center of RDA. Fourteen phenotypic traits were measured in 199 accessions of Cucurbita germplasm. Among these germplasm, 92 accessions of C. moschata, 34 accessions of C. maxima, and 73 accessions of C. pepo were classified using the KASP marker from the Seed Industry Center. It was confirmed that there were five classifications in C. moschata, five in C. maxima and four in the C. pepo. The results of this study provide fundamental data on Cucurbita germplasm and are expected to be useful in breeding programs.

In recent years, an array of studies has focused on ‘translationese’ (i.e., unique features that manifest in translated texts, causing second language (L2) writings to be similar to translated texts but different from native language (L1) writings). This intriguing linguistic pattern has motivated scholars to investigate potential markers for predicting the divergence of L1 and L2 texts. This study builds on this work, evaluating the feature importance ranking of specific translationese markers, including standardized type-token ratio (STTR), mean sentence length, bottom-frequency words, connectives, and n-grams. A random forest model was used to compare these markers in L1 and L2 academic journal article abstracts, providing a robust quantitative analysis. We further examined the consistency of these markers across different academic disciplines. Our results indicate that bottom-frequency words are the most reliable markers across disciplines, whereas connectives show the least consistency. Interestingly, we identified three-word lexical bundles as discipline-specific markers. These findings present several implications and open new avenues for future research into translationese in L2 writing.

On the basis of the investigation of world languages, typologists have summarized the various categories of Numbers of world languages. According to this research result, we can determine which category Korean, Chinese and English plural marks belong to. As a whole, Korean and Chinese belong to general number languages, which are different from English in the nature of plural markers. At the same time, we will find that there are similarities and differences in the plural categories of Korean and Chinese. This paper analyzes from multiple perspectives such as semantics, syntax, and pragmatics, so as to give the prominence difference of Korean and Chinese plural markers. According to the research of library linguistic typology, plural marks should also be related to quantifiers, and there are certain differences in usage and prominence between Korean and Chinese quantifiers. Therefore, we will also summarize the differences in the prominence of Korean and Chinese quantifiers, and then investigate the correlation between Korean and Chinese plural markers and quantifiers. Chinese quantifiers are prominent, but plural marks are not as developed as Korean. Korean quantifiers are not as developed as Chinese, but its plural marks are more developed than Chinese.