The evergreen oak tree, Quercus myrsinaefolia Blume, is not only economically important for wood, medicine, landscape trees, etc., but also becoming more important in terms of ecology due to climate change. However, asexual reproduction was difficult, so this study was conducted to establish the optimum conditions for micropropagation by shoot multiplication. The surface sterilized seeds of Q. myrsinaefolia were successfully germinated in WPM basal medium. BAP (1.0 mg/L) treatment was most effective for inducing multiple shoots. The highest induction rates of adventitious roots from the multiple shoots was shown in the treatment of 1.0 mg/L NAA. Both MS and WPM medium were most effective for growth of multiplied plantlets. For ex vitro acclimatization, the survival rates of multiplied plantlets were 100% in vermiculite and commercial soil. The results of this study can be used for proliferation and supply, and establishment of ex situ conservation of Q. myrsinaefolia elite trees.

참나무와 서어나무는 온대지역에 걸쳐 넓게 분포하고 있으며, 다양한 초식곤충의 먹이원이 되는 것으로 알려져 있다. 이 연구에서는 지리산국립공원 온대림에서 참나무림(반선)과 서어나무림(상선암)에서 초식곤충 활동량을 알고자 하였다. 각 조사지역에 0.1ha의 방형구를 설치하여 참나무와 서어나무의 분포를 확인 한 뒤 개체별로 초식곤충의 활동량인 잎 손상 지수를 측정하였다. 잎 손상 지수는 손상 퍼센트로 환산하여 분석하였다. 측정결과 전체 4,413장의 잎을 관찰하였으며, 참나무림에서는 2,683장, 서어나무림에서는 1,730장의 잎이 조사되었다. 초식곤충의 활동량은 참나무림과 서어나무림에서 각각 차이를 보였으며 서어나무림인 상선암에서 참나무의 식흔량과 서어나무의 식흔량 이 참나무림인 반선보다 모두 높게 나타났다. 이 연구결과를 바탕으로 온대림 우점 수종인 참나무와 서어나무에서 초식곤충의 활동량을 정량적으로 추정할 수 있을 것으로 생각된다.

For centuries, Lentinula edodes called “Pyogo” in Korea has been cultivated as a high-grade edible mushroom in Asia. This is known as medicinal mushroom for cold prevention in oriental medicine. In the world market, this mushroom of Lentinula edodes is introduced as representative edible and medicinal mushroom in Asia. As consumer interest increases, Asian Lentinula edodes market is also growing. There is a record that Korea has already been used as a gourmet food on the royal meal table for the king in the 15th century Joseon Dynasty. In Korea's famous oriental book, it is cooked with many traditional foods because of the pharmaceutical efficacy of this mushroom. For long time ago, difference in cultivation as well as in mushroom growth of Lentinula edodes has studied in Korea. Recent advances in cultivation of selected high quality mushroom studied from substrate optimization using log and sawdust of oak tree. Cultivation of Lentinula edodes strain research controlled the production of large quantity of various substrate conditions. This mushroom mycelia and fruit-body were cultivated in log of oak tree and sawdust substrate cultivation in types of bioreactors. According to compared cultivation method, it presented the optimistic condition for the amount production of various Lentinula edodes strain in Korea.

Background : We examined the inorganic compounds, compressive strength, yield, and functional substances of five steam-treated deciduous tree species to address the problem of the low degradation activity of conventional oak trees in the cultivation of Gastrodia elata in indoor facilities. Methods and Results : With the exception of Ca in cherry blossom tree (CBT), the content of the major inorganic elements (N, P, Mg, K, and Ca) in alternative trees were 1.5 - 3.6, 1.3 - 2.6, 3.3 - 5.3, 4.4 - 7.5, and 0.9 - 3.9 times those in oak (control group), respectively. The content of trace elements (Zn, Cu, Fe, and Mn) were equivalent to or slightly lower than those in oak, depending on tree species. The compressive strength of alternative trees was 9.3%–34.9% lower than that of oak. The compressive strength after steaming was lower in the order CBT (8.0%), mulberry tree (MBT: 7.0%), chestnut tree (CNT: 7.2%), Siebold's crab (SBC: 6.9%), black locust (BLL: 6.1%), and oak (5.4%). The total weight of oak was 893 g in comparison with the 1,107, 902, 952, 813, and 813 g of MBT, CNT, CBT, SBC, and BLL, respectively. The mature tuber and seed tuber ratios were 31%, 34%, 32%, 32%, 34%, and 30%, and 67%, 75%, 68%, 70%, 73%, and 65%, respectively. The yield index increased overall by 1% - 14%. Total weight after steaming increased by 10%, 18%, 14%, 16%, 20%, and 12% in oak, MBT, CNT, CBT, SCB, and BLL, respectively. Mature tuber and seed tuber ratios after steaming increased by 1%, 1%, 0%, 1%, and 2%, and 5%, 3%, 7%, 2%, 5%, and 5%, respectively. There were no significant difference among tree species in drying ratio, hardness, chromaticity for quality comparisons, and steaming. However, the results of functional substance analysis revealed significant differences in gastrodin, gastrodigenin, p-hydroxybenzaldehyde, and ergothioneine according to tree species. Conclusion : From the above results, we may believed that five tree species including MBT might have useful as alternative to oak when the cultivation of G. elata in Indoor Facilities.