Streptozotocin(STZ)으로 유도된 당뇨 흰쥐에게 연교의 에탄올 추출물을 1일 1회 7일간 1,000㎎/㎏.b.w의 용량으로 투여 후 glucose함량과 이에 관여하는 효소(glucokinase(GK), glucose-6-phosphatase(G-6-Pase), glucose-6-phosphate dehydrogenase(G-6-PDH)활성과 glycogen 함량, triglyceride(T.G), total cholesterol등의 지질대사에 관여하는 물질들의 함량을 측정한 결과 연교 에탄올 추출물 투여군이 glucose, T.G, total cholesterol 등의 함량과 G-6-Pase 활성이 유의적인 감소를 나타내었으며 glycogen 함량과 GK의 활성이 유의적인 증가를 나타내었다. 이와 같이 연교 에탄올 추출물을 1,000㎎/㎏.b.w을 당뇨 흰쥐에게 투여한 결과 혈당저하, 지질대사의 개선 효과를 갖는 유효성분을 함유하고 있음을 알 수 있었다.

In present study, we investigated the antidiabetic effect of Momordica charantia(as well known “bitter melon”). This study was conducted to determine antidiabetic mechanism of Bitter Melon Extract (BME). We measured blood glucose, insulin, glucagon level in a Sprague-Dawley rat model of high-fat diet/streptozotocin(HFD/STZ)-induced diabetes. Five experimental groups were used: normal, HFD/STZ, BME 62.5 mg/kg HFD/STZ, BME 125 mg/kg HFD/STZ and BME 250 mg/kg HFD/STZ. BME was orally administered to the rats every other day for 9 weeks. Results showed that fasting blood glucose levels were significantly lower in the BME 125 mg/kg(150.17 ± 20.22 mg/dL) and 250 mg/kg(124.17 ± 22.17 mg/dL) groups than in the vehicle group(188.83 ± 26.63 mg/dL)(p<0.05). In addition, glucagon levels were lower in the three BME treatment groups than in the vehicle group(p<0.05). Oral glucose tolerance tests revealed that the BME 250 mg/kg group had significantly(p<0.05) reduced 120-minute blood glucose levels and areas under the curve. Our results suggest that BME induces antidiabetic effects via the reduction of glucagon and blood glucose levels.

This study was conducted to investigate the effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention in a streptozotocin-induced diabetes rat model. A total of 28 male rats were divided into four dietary groups and fed a commercial diet (A), commercial diet plus induced diabetes by a streptozotocin (STZ) injection (B), induced diabetes by STZ plus medicinal crop extracts(I&H®) diet (C), and medicinal crop extracts (I&H®) diet (D). Immunoblotting analyses revealed cytokine expression, and ELISA analyses revealed immunoglobulin E and nitric oxide production. As a results, the tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) as a inflammatory cytokine were decreased. Interleukin-6 (IL-6) and signal transducer and activation of transcription 3 (STAT3) cytokine related in diabetes expression through JAK/STAT3 pathway were also decreased. Furthermore, immunoglobulin E and nitric oxide production were decreased in the serum and lens, respectively. These results suggest that Lycii fructus and Astragalus membranaceus mixed extracts provide positive effects on immunomodulators and prevention in diabetes and eye disease complications.

곤달비 메탄올 추출물은 100mg/kg 및 200mg/kg의 경구투여로 STZ로 유도된 당뇨병의 제반 증상을 완화하였다. 즉, STZ의 투여는 흰쥐의 체중, 수분 섭취, 식이섭취를 증가시켰으며, 혈당량, 혈청 triglyceride 량, 동맥경화 위험지수를 증가시켰으나, 곤달비 추출물의 투여는 이러한 당뇨병에 관련된 증상을 개선하였다. 이와 더불어, 위의 당뇨병 모델 쥐에서 당내성 실험에서도 곤달비 추출물이 현저한 효과를 나타내었으므로 혈당의 세포내 이행을 촉진시키는 것으로 확인되었다. 이상과 같은 결과로부터 곤달비 추출물은 당뇨병 및 그에 따르는 고지혈증에 유효하였으므로 당뇨병 질환의 완화를 위하여 사용될 수 있는 산채 식품인 것으로 나타났다. 이러한 결과를 바탕으로 곤달비의 활성 물질의 입증과 그 이용에 관한 계속적인 연구가 필요한 것으로 생각된다.