Honey production from approximately 1.7 million colonies owned by around 21 thousand beekeepers was almost 36 thousand M/T in Korea. Pollen has used as a food and medicine from before the Joseon Dynasty period in Korea. Pollen grains such as acorn (Quercus acutissima), actinidia (Actinidia arguta) and green tea (Camellia sinensis) are popular in the markets in Korea. But stiff pollen wall hindered dissolution of polysaccharides and lowered extraction efficiency. In the present study, we measured the antioxidant activity and the total polyphenol content from the pulverized and lyophilized green tea pollen grains inoculated with 6 kinds of fungi to confirm the husk removal effect. The total polyphenol content of green tea pollens was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in green tea pollen germinated with A. mellea. The total polyphenol content of the lyophilized green tea pollens germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH free radical scavenging method showed that the lyophilized green tea pollen grains germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized green tea pollen grains germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of green tea pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from green tea pollen grains inoculated with A. mellea.

In this study, we examined the composition of catechins, theaflavins and alkaloids in leaves during processing to fermented black tea, which is produced by withering, roll breaking, and fermentation of Korean Yabukida tea plant. In addition, we determined the optimal conditions for the production of fermented black tea. The average moisture content in fresh leaves was 70.85%, which dropped to 3.07% in fermented black tea at the last stage of production. When the leaves were analyzed by HPLC, seven types of catechins, four types of theaflavins and three types of alkaloids were identified. The levels of catechins, theaflavins, and alkaloids were then evaluated after being processed into fermented tea. From these experiments, we found that the level of theaflavins, which determines the property of the tea, increased during fermentation. This effect resulted from the change in EGCG, ECG, EGC, EC during the process of fermentation. We also found that the maximal amount of theaflavins was created after 1-2 hours of fermentation. Thus, our results imply that the best condition for producing fermented black tea would be to ferment for 1-2 hours.

Green Tea consists of 15-30% catechins (a type of polyphenol), which act as super antioxidants, inhibitory action against aging process. Antioxidants fight radical-free oxygen, an agent which can begin the process of cancer by damaging essential body chemicals and harming DNA. This study was peformed to develop the elderly diet using Green Tea as an inhibitory action against aging process. Nokchaborijook (Baley gruel added rice powder and Green Tea) was manufactured by various levels of Green Tea(1, 4, 7%) and the grinding period(15, 30, 45 sec). The optimum levels of added Green Tea and grinding periods on Nokchaborijook were determined with the results of sensory evaluation by response surface methodology and analysis of composition. The Anti-oxidant Vitamin A, C, E and Flavonoid were increased with increased levels of Green Tea and grinding periods be decreased. As the levels of Green Tea and grinding periods were increased, the green color and penetrated force became stronger. Among the sensory attributes, Bitterness, Green Tea Flavor and Hashness were greater depending on increasing Green Tea. Jujube flavor was greater relying on decreasing Green Tea. Greenness and spreadability of particles were increased as both the amounts of Green Tea and the grinding period were increased. This result was used to determine the optimum conditions of adding levels of Green Tea and grinding periods. The optimum conditions of Nokchaborijook was established as adding of 5.8% Green Tea grinded for 15 seconds.

The acaricidal activity of extracts of Gleditsia japonica var. koraiensis, Camellia sinensis, Cinnamomum cassia, Lantana camara and their mixtures against the tea red spider mite, Tetranychus kanzawai was evaluated. Treatments with mixtures 3 and 1 were effective against the adult females of T. kanzawai and yielded 82.0% and 77.3% adulticidal activities at 7 days after treatment, respectively. Treatment with mixture 2 revealed 68.0% adulticidal activity. Generally, the acaricidal activity of the single treatment of each plant extract was lower than the mixtures. The adult females of T. kanzawai treated with mixtures 3 and 1 produced only 24.0~29.6% as many eggs as control females did. The residue of the plant extracts tested had low adulticidal activity (16.7~31.3% at 7 days after treatment). All the plant extracts tested exhibited no remarkable toxic effect to the eggs of T. kanzawai. Treatments of mixtures 3 and 1 were effective against nymphs of T. kanzawai and led to emergence rates of 16.7 and 22.0%, respectively. These results suggest that mixtures 3 and 1 might be used for the control of T. kanzawai in tea fields.

Based on double pseudo-testcross theory, a population of 76 F1 clones, which were derived from a cross of China type tea plants (Camellia sinensis var. sinensis) with a Korean tea cultivar, ‘Kemsull’ for female parent and a Japanese tea cultivar, ‘Houshun’ for male parent, was used to construct a genetic linkage map with AFLP markers. Totally, 2,360 markers were detected by 26 pairs of primers and 90.8 markers for each pair on average. Among these, 481 markers (20.3%) were polymorphic, 392 markers (81.5%) of which showed Mendelian segregation ratio (p=0.01). Of these Mendelian segregated markers, 139 (35.5%) were segregated in 3:1 ratio and 253 (65.5%) were segregated in 1:1 ratio. The construction of AFLP molecular marker based linkage map were carried out by Joinmap 4.0 version. The linkage map of ‘Kemsull’ contained 227 markers which distributed into 18 linkage groups. The linkage map of ‘Kemsull’ covered 1,382.2 cM with the average distance between two markers of 6.0 cM. The linkage map of ‘Houshun’ contained 154 markers which were distributed into 17 linkage groups and were spanned with the total map length of 1,540.9 cM and the average distance between two markers of 10 cM. However, these AFLP markers were not distributed evenly and further even saturation is additionally required.

Plant biomass is a huge carbon-complex that has potential as a nutrient. Therefore we extracted and separated useful materials for plant growth from tea leaf and stem. The pre-treatment process including high temperature (200 °C) and pressure (20-40 kgf/cm2) was treated for several minutes and extracted at 120 °C for 30-60 minutes. After that the chemical compositions and ingredients were analyzed from that plantnutrient. As a result of mineral contents, calcium and magnesium concentrations are higher than other minerals. Also the result of carbohydrates analyses has shown that the sugar oligomer consists of xylose(95.3%) and glucose(4.7%), and the sugar monomer consists in the order of xylose (52.7%) > manose (22.8%) > arabinose (10.8%) > galactose (10.2%) > glucose (3.5%). Before applied to field, in vitro plant growth system and formulation were examined. To evaluate the effect of the nutrients, both strawberry green-house and persimmon fields were used in this test. The treated persimmons were heavier than controls scored at 13-22%. In addition, the storageperiod was extended in the treated strawberries. Interestingly in the treated strawberry, the contents of polyphenols were increased (38-57%). These results suggest that the plant-nutrient can afford to help for plant growth and storage, and it can be substituted for other commercial nutrients. In conclusion, this plant-nutrient may help to extend eco-friendly or organic farming in Hadong-gun area.

Tea plants growing under natural condition meet various stresses. The tea plants often subjected to adverse condition like low temperature and high light intensity during overwintering. Because tea plant is adapted well to low light intensity, higher light intensity might induce negative effects on tea growth at open field (exposed habitat). We investigated tea plant planted with the seeds of wild tea trees growing at Unsu-ri, Hwagaemyun, Hadong-gun on Nov. in 2008. The experimental field for juvenile tea plants was located in the hill neighboring GSNU at Jinju, Korea. We divided the field into open, semi-shaded, and shaded condition. Light intensity (PAR) at open condition was two times and twenty times higher than at semi-shaded and shaded condition, respectively. Growth of tea plants was highly dependent on light condition, the plants at open field was extremely poor as compared to plants grown at shaded field. Height of plants at open, semi-shaded, and shaded field were 14, 17.3, and 17.8 cm, respectively. Leaf size was also greater in plants grown at shaded field. Photosynthesis-related chlorophyll fluorescence (quantum yield) was also higher in shaded plants. Fv/Fm value of plants grown at open fields was lower by 50% as compared to that of the plants grown at shaded field. Chlorophyll content measured by SPAD meter also indicated higher value in open field plant than shaded plants. Our results suggested the photoinhibition during overwintering period strongly affects the growth juvenile tea plants because there was no significant difference in soil moisture content between open and shaded condition.

전국 차나무 자생지에서 차나무를 수집하여 조성된 클론보존원의 630클론 중 생육이 왕성하고 내한성이 높은 차나무 31계통을 선발하였다. 선발된 31계통 중 최종적으로 3계통을 선발하여 2006년부터 2008년까지 전국 6개 지역에서 지역적응성 및 영양계 비교시험을 실시하였다. 그 결과 내한성이면서 다수성을 가진 영양계 차나무 "한광"을 최종 선발하였다. "한광"은 생육이 왕성하여 새싹수는 대비품종인 야부기다에 비하여 27.6% 많았고 백아중은 47.9