친환경 유기농자재를 이용하여 잿빛곰팡이병균의 포자 발아, 균사생장 억제효과를 기내에서 조사하였다. 그 결과 잿빛곰팡이병의 균사생장 억제 효과는 Bacillus subtilis를 주성분으로 하는 제제가 100%의 억제효과를 보였으며, 그 외의 제제는 20% 이하의 범위에서 억제효과를 나타내었다. 포자발아 억제효과는 유기황 수화제 2종('BTB', '황스타')이 각각 97.7%, 92.3%으로 나타났다. 균사생장 억제와 포자 발아억제에 모두 효과를 보이는 제제는 없었다. 따라서 잿빛곰팡이병 방제를 위해서는 포자발아 억제를 위한 방제와 균사생장억제를 위한 제제를 각각 처리하여야 할 것으로 사료되었다. 아울러 사물 기생성을 가진 잿빛곰팡이병의 방제를 위해서는 잔재물에서 생장을 막는 것이 중요하며 이를 위해 예방을 전제로 한 방제의 경우 유기황을 주성분으로 하는 제제가 적용 가능할 것으로 생각된다.

The insecticidal effect for several commercially available environment friendly agricultural materials (EFAMs) is investigated to Cnaphalocrocis medinalis, Narana aenescens, Pseudaletia unipuncta and Parnara guttata on rice. In the insecticidal effects for 2-3rd instar larvae of lepidopteran after treatment with 19 EFAMs, more than 95% of mortality rate showed 5 EFAMs in C. medinalis, 12 EFAMs in N. aenescens, 9 EFAMs in P. unipuncta, and 14 EFAMs in P. guttata, respectively. Among 4 EFAMs selected for C. medinalis in indoor, more than 70% in control effect is seen EFAM-B(85.2%), EFAM-I(79.1%) and EFAM-M (78.3%), respectively, in rice field. The insecticidal effect for EFAMs is investigated to Nilaparvata lugens, Laodelphax striatellus and Nephotettix cincticeps on rice. In the insecticidal effects for 2-3rd nymph and adult after treatment with 19 EFAMs, more than 95% of mortality rate showed 5 EFAMs in N. lugens adult, 7 EFAMs in N. lugens nymph, 6 EFAMs in L. striatellus, and 5 EFAMs in N. cincticeps, respectively. Insecticidal effect for N. lugens was sustained until 10 day in rice treated with 7 EFAMs selected in indoor. Among them, EFAM-L, EFAM-P and EFAM-R was sustained in low density until 27 day. But EFAM-D, EFAM-L, EFAM-N and EFAM-S showed high density until 27 day and additional control was needed.

Many kinds of environment friendly agricultural materials (EFAMs) were used for the plant protection, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Natural enemies might be used for control of insect pest with EFAMs at the same space and time. For testing the toxicity of EFAMs against to natural enemies, 10 EFAMs were selected and tested in small greenhouses and farm"s fields. In case of Harmonia axyridis larva, there was no predacious activity against cotton aphids in the block with EFAMMo L, EFAMPE D, EFAMME G and EFAMAE A, otherwise, aphid population was rapidly decreased in the control block without EFAMs. Both of Trialeurodes vaporariorum and Encarsia formosa population were slightly decreased in the block with EFAMs. There were significantly decreased in the block with IEFAM C, FEFAM D, EFAMSM Band EFAMPE D compared with control area. Especially, there was significantly maintained a very low population, both T. vaporariorum and E. formosa in the block with EFAMMo C. The parasitized ratio of Aphidius colemani is also very low in the block with EFAMs compared with control area. In the block with IEFAM C, FEFAM D, EFAMPE D and EFAMMo L, there were significantly different with control area in the number of mummies. Otherwise, in case of Diglyphus isaea and Liriomyza trifolii, there was no significantly different between blocks with and without EFAMs. In the block with EFAMMo C, Tetranychus urticae and Phytoseiulus persimilis populations were significantly maintained a very low level. However, the population of T. urticae was increased, but P. persimilis was decreased in the block with EFAMMo L.

The multicolored Asian ladybird beetle (Harmonia axyridis) has been commonly used with biological control agents for control of several kinds of aphids in agroecosystems. Also, environment friendly agricultural materials have been commonly applied in crop fields because the government held down pesticide application and environment friendly agricultures are gradually increased with consumer"s desires. The multicolored Asian ladybird beetles may be directly or indirectly under the influence of environment friendly agricultural materials in crop fields. The insecticidal environment friendly agricultural materials (IEFAMs) might be safe against each developmental stage of multicolored Asian ladybird beetle. Fungicidal environment friendly agricultural materials (FEFAMs) had a miner effect to each developmental stage of multicolored Asian ladybird beetle with the exception of FEFAM A. Environment friendly agricultural materials contained useful soil microorganisms (EFAMSMs) C and H might be down the hatching rate of eggs, and EFAMSM A and F had a killing effect to 1st instar of lady beetles. Environment friendly agricultural materials contained plant extracts (EFAMPEs) A and D might be suffered effect a deathblow of egg hatching with lady beetles. Otherwise, there was a miner effect to lady beetles with the rest of tested environment friendly agricultural materials.

Many kinds of environment friendly agricultural materials were used for the insect pest control and the control of plant diseases, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Phytoseiulus persimilis might be used for control of two-spotted mites with environment friendly agricultural materials at the same time. For testing the toxicity of environment friendly agricultural materials against to P. persimilis, 61 environment friendly agricultural materials were selected by material contents and using methods. When environment friendly agricultural materials were directly sprayed on P. persimilis, IEFAM C, FEFAM A, EFAMSM A, D, EFAMPE A, EFAMCh B, EFAMME A, and EFAMMo C killed over 90%. However, there was no effects to FEFAM C, D, EFAMSM C, EFAML A, EFAMME C, E, H, J, EFAMMo G and I against P. persimilis. P. persimilis adults were not survived in vial for 48 hours after sprayed and dried with the environment friendly agricultural materials, for examples, EFAMSM I, EFAMME A, EFAMMo A, C, and I. Otherwise, EFAMCh C and EFAMMo B were no effects to P. persimilis. Some environment friendly agricultural materials are of different qualities, and consequently test of their toxity have to necessary.

For the biological control of the greenhouse whitefly, aphids, American leaf-miners, Encarsia formosa, Aphidius colemani, Diglyphus isaea, and Dacnusa sibirica were used as biological control factors. Otherwise, many kinds of environment friendly agricultural materials were also used in the kindly environment friendly fanning. For testing the toxicity of environment friendly agricultural materials against to insect parasitoids as biological control factors, 61 environment friendly agricultural materials were selected by using methods and periods with insect parasitoids in the greenhouses. Environment friendly agricultural materials were sprayed to mummies and adults of E. formosa and A. colemani, and adults of Diglyphus isaea and Dacnusa sibirica in laboratory condition. Emergence rates were checked during 12 days after spray to mummies of E. formosa and A. colemani, and survival rates were at 24 and 48 hours after viral test for adult parasitoids, with 10% sugar solution. Emergence rates of E. formosa mummies were inhibited by sprayed the fungicidal environment friendly agricultural material (FEFAM) E and the environment friendly agricultural materials contained molybdenum (EFAMMo) G as 0.4 and 2.7%, respectively. E. formosa adults were not survived in vial for 48hours after sprayed and dried with the environment friendly agricultural materials contained plant extract (EFAMPE) J and the EFAMMo C. The environment friendly agricultural materials contained microelements (EFAMMEs) B and EFAMPE H, environment friendly agricultural materials contained useful soil microorganisms (EFAMSMs) H, FEFAM A and FEFAM D were recorded low survival rates of E. formosa adults with 6.7, 13.3, 13.3, 20.0 and 20.0, respectively. In case of the spraying environment friendly agricultural materials to mummies of A. colemani there were low emergence rate from mummies to adults as around 20s % with water spraying as control. A. colemani adults were not survived in vial with EFAMMo C. EFAMPE J, EFAMPE F, EFAMPE H, EFAMSM H were recorded low survival rates under 20% as 6.7, 13.3, 13.3 and 13.3, respectively. D. isaea and D. sibirica adults were not survived in vial with EFAMPE J. EFAMMo C was 53.3% of survival rates in 48 hours vial tests. D. isaea and D. sibirica were not affected by environment friendly agricultural materials compared with E. formosa and A. colemani.

The SS (Bacillus subtilis 10%), WS (Microbial extract 70%), and DS (Sulfur 78%) agents were selected by mycelial growth inhibitory effect test against kiwifruit soft rot pathogen (B. dothidea) with 11 kinds of environment-friendly agricultural materials on PDA medium for 10 days. They showed at 94.2%, 65.2%, 58.9%, respectively. The control value of WS and SS agents were better than DS in storage experiment. It was effective SS and WS single application, DS-WS and WS-SS alternate application in the field trial.

We studies a model for management of pepper anthracnose based covering method and spraying system in field. 1. Among 82 organic fungicides, 42 materials showed most effective inhibition against mycelia growth of the Colletotrichum acutatum in vitro. 23 formulated biocontrol agents were chosen to control the disease from 42 biocontrol agents in greenhouse. In the end, five kinds (2 plant extracts, 2 biopesiticides, 1 Bordeaux mixture) were selected from 23 materials in the field. 2. The mulching materials of bed covering in fruit season were thin non-woven fabric sheet and black plastic. The use of a fabric sheet was reduced the spread of anthracnose as compared to the plastic covering. 3. The application with the chosen materials was reduced 34% of anthracnose for 7 times sprays to planting 70 days as compared to the untreated control. In yield, nonwoven fabric sheet with formulated biopesticides was increased 17% than black plastic. 4. This result indicated that the developed biocontrol strategy could be an effective and economic crop protection system in organic pepper cultivation field.

Environment friendly agricultural material products (EFAMs) in current market were evaluated for their insecticidal or acaricidal activities. EFAMs were evaluated against Tetranychus urticae, Plutella xylostella, Myzus persicae and Nilaparvata lugens in spray method. Several EFAMs indicated high control value in spray method. Hatchability of T. urticae was relatively high after EFAM treatment. some materials show high insecticidal activities against P. xylostella larvae but not against eggs. Few meterials showed more than 80% insecticidal activities against M. persicae and N. lugen. The control value of more than half the materials for pest control was lower than 60%. EFAMs containing S. flavescens, neem oil and Rape showed higher control value

This study was carried out to development the environment-friendly control method of Myzus persicae using Beauveria bassiana and Environment-friendly agricultural materials (EFAM). Germination rate the dried conidia of B. bassiana was 90 percent at 24-27 ˚C temperature condition. Mortalíty of M. persícae was 56. 7% at 1 X1 07 cfu/ml conidia concentration. Four EFAM such as Pachungtan, Wangjungwang, Newbiodakill, Muginchon were low toxicity to B. bassiana growth. Among them, Muginchon (derris extract) and Wangjungwang (neem extract) could have synergistic effect to aphid mortality when mixing treat with B. bassiana, which protection value was 99% in 3 days after treat.