인삼 뿌리썩음병균(Cylindrocarpon destructans)과 뿌리혹선충(Meloidogyne spp.)은 국내 인삼(panax ginseng C. A. Meyer) 연작장해의 주요인으로, 인삼 생산성 향상을 위해 방제가 필요하다. 본 연구는 새로 개발한 액제 훈증성 토양 소독제를 살포하며 동시에 비닐피복이 가능한 트랙터부착형 토양 소독기를 이용하여 dimethyl disulfide (DMDS)를 처리하였을 때, C. destructans와 뿌리혹선충의 방제효과를 분석하고, 시작기의 성능을 확인하기 위해 수행하였다. 토양 소독기를 이용하여 인삼 재배 포장에 DMDS를 처리한 후 비닐이 피복된 상태에서 5주간 훈증하였다. 토양 소독기의 성능은 약제 살포량 오차 2.5%, 작업능률 0.9h/10a로 40%의 노력절감 효과가 있는 것으로 나타났다. 또한, 처리 전후 C. destructans 의 밀도를 분석한결과 82.5%의 밀도 억제 효과가 있는 것으로 나타났으며, 뿌리혹선충 방제효과는 100%로 나타났다. 따라서, 본 토양 소독기 시작기를 이용하여 능률적으로 DMDS를 처리할 수 있으며, C. destructans와 뿌리혹선충의 방제효과를 볼 수 있는 것으로 판단된다.

양송이 버섯재배 시 발생하는 긴수염버섯파리(Lycoriella mali)의 효과적인 생물학적 방제를 위하여 곤충병원성 선충인 Steinernema carpocapsae를 이용하였다. 국내에 유통되고 있는 두 제품의 농도는 각각 m2 당 6 × 104과 2.5 × 105이었다. 양송이 버섯시설 내 버섯배지와 상토 에서의 긴수염버섯파리 유충 밀도를 조사한 결과 각각 30 cm2 내 평균 마리 수는 0.8마리와 22.2마리로 상토에서 주로 서식하였다. S. carpocapsae 1회 처리 후 방제효과는 처리 14일 후에 가장 높았다. S. carpocapsae 6 × 104 농도 제품의 2회 처리에 따른 긴수염버섯파리 방제효과는 51.9% 였으며 2.5 × 105 농도 제품의 2회 처리 후 방제효과는 96.8%로서 2.5 × 105 농도가 6 × 104 농도 보다 방제효과가 우수하였다. 살충제인 diflubenzuron 수화제의 방제효과는 50% 이하로 낮은 수준이었다.

소나무재선충병의 나무주사는 살선충제를 중심으로 실시되어 왔으며, 매개충인 솔수염하늘소와 북방수염하늘소의 방제는 주로 항공방제나 지상방제를 통하여 이루어졌다. 본 연구에서는 나무주사를 통하여 매개충(솔수염하늘소)을 방제하기 위한 연구로 Abamectin+Acetamiprid ME, Thiamethoxam DC약제의 나무주사시 솔수염하늘소의 약효발현농도, 수목내 약제의 이동특성 및 치사율 등에 대해서 조사하였다. 실내 약효발현농도 시험에서는 Abamectin+Acetamiprid ME, Thiamethoxam DC 모두 0.1ppm 이상의 농도에서 평균 6일 경과 후 100.0% 치사하는 특성을 나타내었다. 또한 야산에서 나무주사를 통하여 솔수염하늘소에 대한 효과검토 결과, Abamectin+Acetamiprid ME, ThiamethoxamDC 모두 처리 60일 후 90.9%의 치사율(가지섭식 14일 후 기준)을 나타내었으며, 죽지 않는 개체는 가지의 약제침투특성차이에 의한 것으로 판단된다.

총채벌레는 저항성 해충군이 가지고 있는 크기가 작고 산란수가 많으며 세대기간이 매우 짧은 특성을 가지고 있다. 또한, 이동능력이 우수하고, 다양한 령기가 혼재하고 있으며, 꽃과 잎 등 식물체뿐만 아니라 토양에서 생활환을 완성하는 복잡한 생태를 가지고 있다. 이러한 총채벌레의 특성으로 인하여 시설 내 총채벌레의 방제는 어려운 실정이며, 동일계통의 작용기작을 갖는 약제들의 단조로운 패턴의 약제사용으로 인하여 그 방제효과는 떨어지고 있다. 본 연구에서는 총채벌레의 효과적 방제를 위한 실내 처리방법별 약효비교와 시설포장에서 처리 패턴별 방제효과 및 작용기작에 따른 체계적인 약제처리 에 대한 방제효과 등에 대한 연구를 수행하였고 이를 기반으로 총채벌레 방제체계를 제안하였다. 실내에서 약제별 총채벌레의 약충과 성충에 대한 약효를 통하여 약제특성 및 총채벌레 밀도가 높지 않은 초기 사용이 가능한 약제와 총채벌레 밀도가 높은 시기에 처리할 수 있는 약제를 선발하였고, 이를 기반으로 포장에서 처리간격 및 혼용살포에 대한 효과를 검정하였다. 본 시험결과들을 기초로 2016년 ㈜경농에서는 3가지 이상의 다른 작용기작의 약제를 밀도가 높을 경우 3~5일간격으로 3회 이상 연속하여 살포하는 3!3!3! 총채벌레 관리프로그램을 제안하였고 지속적으로 관리프로그램을 개선해 나갈 예정이다.

Road-map for the environmental friendly integrated pest management (IPM) of insect pests was drawn up on the strawberry vinyl-houses of farmer’s field. Major insect pests were occurred Tetranychus urticae and Aphis gossypii during the strawberry plant seeding in the vinyl house and open field. Also, same insect pests were occurred in the vinyl house during harvesting season of strawberry. For the control of T. urticae and A. gossypii, Phytoseiulus persimilis and Aphidius colemani as natural enemies were input to the vinyl house, respectively. However, because these natural enemies could not control insect pest populations, acaricide and insecticide were sprayed. Then natural enemies were input again in the vinyl house. Natural enemies could not endure the intense cold and differences of temperature and relative humidity between day and night during strawberry harvesting season. So, their behavior and control activity of pests were more decrease than pests. Firstly, natural enemies are input in the vinyl house during the early breeding season of strawberry, secondly, acaricides and insecticide are sprayed for the control of mites and aphids, respectively, during the middle breeding season in the hard winter. Finally, natural enemies are re-input in the vinyl house during the middle and late breeding season.

Many kinds of environment friendly agricultural materials (EFAMs) were used for the plant protection, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Natural enemies might be used for control of insect pest with EFAMs at the same space and time. For testing the toxicity of EFAMs against to natural enemies, 10 EFAMs were selected and tested in small greenhouses and farm"s fields. In case of Harmonia axyridis larva, there was no predacious activity against cotton aphids in the block with EFAMMo L, EFAMPE D, EFAMME G and EFAMAE A, otherwise, aphid population was rapidly decreased in the control block without EFAMs. Both of Trialeurodes vaporariorum and Encarsia formosa population were slightly decreased in the block with EFAMs. There were significantly decreased in the block with IEFAM C, FEFAM D, EFAMSM Band EFAMPE D compared with control area. Especially, there was significantly maintained a very low population, both T. vaporariorum and E. formosa in the block with EFAMMo C. The parasitized ratio of Aphidius colemani is also very low in the block with EFAMs compared with control area. In the block with IEFAM C, FEFAM D, EFAMPE D and EFAMMo L, there were significantly different with control area in the number of mummies. Otherwise, in case of Diglyphus isaea and Liriomyza trifolii, there was no significantly different between blocks with and without EFAMs. In the block with EFAMMo C, Tetranychus urticae and Phytoseiulus persimilis populations were significantly maintained a very low level. However, the population of T. urticae was increased, but P. persimilis was decreased in the block with EFAMMo L.

The multicolored Asian lady beetles (Harmonia axyridis) has characteristic color patterns, which show great variability within species. Up to now, it has been well known that main factors affected on individual color pattern variations in the population of H. axyridis are external, physical, and environmental characteristics. Indeed, there is as yet no evidence to indicate whether the variation is genetic or environmental factors. Also the factors which produce this variation are unknown in this species, although it is suspected that much of the variation is under genetic control. However, the genetic relationships among many of color types were investigated by observing the progeny of each particular pairs. It is worth mentioning a few particular breeding cases to illustrate certain facets of variability, and to indicate examples suitable for genetic analysis of the color pattern variation.

The external ultrastructural morphology and distribution of antennal sensilla and hair-pencils of Palpita indica (Saunder) were examined by scanning electron microscopy. The antennal flagellum of male and female has six types of sensilla: long trichodea, short trichodea, chaetica, coeloconicum around spines, camaniformia and styloconica. There are sexual differences in antennae of P. indica. The female antenna has a coeloconicum sensillum without spines nearby the long trichodea sensilla on ninth flagellomere. Otherwise, auricillica sensilla are distribute on the basal segment of male antenna. These structures are not showed in the female antenna. There are a lot of pores on the hair tufts of the male hair-pencils.

The multicolored Asian ladybird beetle (Harmonia axyridis) has been commonly used with biological control agents for control of several kinds of aphids in agroecosystems. Also, environment friendly agricultural materials have been commonly applied in crop fields because the government held down pesticide application and environment friendly agricultures are gradually increased with consumer"s desires. The multicolored Asian ladybird beetles may be directly or indirectly under the influence of environment friendly agricultural materials in crop fields. The insecticidal environment friendly agricultural materials (IEFAMs) might be safe against each developmental stage of multicolored Asian ladybird beetle. Fungicidal environment friendly agricultural materials (FEFAMs) had a miner effect to each developmental stage of multicolored Asian ladybird beetle with the exception of FEFAM A. Environment friendly agricultural materials contained useful soil microorganisms (EFAMSMs) C and H might be down the hatching rate of eggs, and EFAMSM A and F had a killing effect to 1st instar of lady beetles. Environment friendly agricultural materials contained plant extracts (EFAMPEs) A and D might be suffered effect a deathblow of egg hatching with lady beetles. Otherwise, there was a miner effect to lady beetles with the rest of tested environment friendly agricultural materials.

Many kinds of environment friendly agricultural materials were used for the insect pest control and the control of plant diseases, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Phytoseiulus persimilis might be used for control of two-spotted mites with environment friendly agricultural materials at the same time. For testing the toxicity of environment friendly agricultural materials against to P. persimilis, 61 environment friendly agricultural materials were selected by material contents and using methods. When environment friendly agricultural materials were directly sprayed on P. persimilis, IEFAM C, FEFAM A, EFAMSM A, D, EFAMPE A, EFAMCh B, EFAMME A, and EFAMMo C killed over 90%. However, there was no effects to FEFAM C, D, EFAMSM C, EFAML A, EFAMME C, E, H, J, EFAMMo G and I against P. persimilis. P. persimilis adults were not survived in vial for 48 hours after sprayed and dried with the environment friendly agricultural materials, for examples, EFAMSM I, EFAMME A, EFAMMo A, C, and I. Otherwise, EFAMCh C and EFAMMo B were no effects to P. persimilis. Some environment friendly agricultural materials are of different qualities, and consequently test of their toxity have to necessary.