When researchers performed intestinal microbiota analysis using fecal samples, sample preparation processes are not standardized as of now. In particular, contrary to lab conditions, there are various factors hard to be controlled when sampling livestock feces on the farm. In this study, we wanted to know the influences of sample preparation conditions on fecal microbiota. We designed an experiment considering various sample preparation conditions (sample origin: rectum and ground; transporting condition: lysis buffer-treated, dry ice, ice, and room temperature; delayed times to extract DNA: 0, 1, 6, and 24 h) that can occur during microbiota analysis using cattle feces. First, we investigated the influences of sample origin, and microbial diversity (observed OTUs, p<0.001) was significantly higher when fecal samples were obtained from the ground than from the rectum and the principal coordinates analysis plot showed that samples were divided into two groups by origin. When we investigated the influences of transporting and storage conditions, observed OTUs (p<0.05) was significantly higher when samples were transported at room temperature than other conditions, and microbiota was affected by transporting and storage conditions. Finally, we investigated the effects of delayed time before DNA extraction from frozen fecal samples on microbial composition. Although this factor did not have a significant influence on microbial diversity, multidimensional scaling revealed its impact on microbial composition. Our findings will contribute to establishing an optimal procedure for microbiota analysis using farm-housing livestock feces.

본 논문에서는 농장동물을 활용한 동물매개치료 문헌연구를 통해 프로그램 적용 사례를 살펴보고, 관련 연구와 활동에 필요한 자료는 제공하고자 한다. 국내외 농장동물을 활용한 동물매개치료 연구를 보면 반려동물을 매개로 한 동물매개 치료와 마찬가지로 우울 감소, 불안감소, 사회성 향상 검증되었고, 특히 정신 질환이나 자 폐 같은 다양한 정신문제에 긍정적인 효과가 검증되었다. 하지만 과학적인 연구가 아직 부족한 실정으로 대상자와 대상 질환에 맞는 농장동물매개치료 프로그램의 개발연구가 필요하다.

The experiment was undertaken to measure the potential impact of animal welfare on the pork quality under two different farming systems: C1 and C2, two conventional farms; W, an animal welfare farm. Ten pigs (Landrace × Yorkshire × Duroc) of live weight 110 kg were randomly selected from each treatment, and slaughtered under uniform conditions. The left loins were collected, and subsequently analyzed for proximate composition, meat quality traits, fatty acids, free amino acids, and antioxidative dipeptides. Pork from the welfare farm (W) had greater back-fat depth and fat content, but the values were not significantly different from C1 or C2. The W loins had significantly lower moisture contents and pH values compared to C1 or C2. The C2 loins showed highest contents of unsaturated fatty acids, whereas C1 had higher polyunsaturated fatty acids as compared to W. The treatments did not show any significant differences in the contents of free aspartic acid. Anserine (an antioxidant dipeptide) from the W loins was significantly lower than that from the conventional farms. The results indicate that pork quality characteristics from animal welfare-farmed pigs were not different from those of conventional farms. Therefore, it would be appropriate to evaluate the industrial value of animal welfare livestock only at the stage of pig production, rather than the meat quality of pork.

본 연구는 가축분뇨의 냄새 저감용 제제의 효율적 활용 및 관리를 위하여 국내에서 유통되고 있는 환경개선제의 동향 및 냄새제거 효능을 조사하고자 실시하였다. 사료첨가용 제제는 전체 제품의 80%를 차지하였고, 다음으로 분뇨살포용 제제 그리고 음수첨가용 제제 순이었다. 대부분의 제제들은 0.1-0.2% 사용을 권장하였으며, 전체 제품의 71%를 차지하였다. 환경개선제 유효성분 중 가장 많은 원 료는 생균으로 전체 제제의 85%를 차지하였고, 다음으로 규산염, 허브 그리고 유기산과 효소로 구성된 제제 순이었다. 환경개선제용 생균제로 이용되는 미생물들은 Bacillus coagulans, B. fermentum, B. thuringiensis, B. licheniformis, B. subtillis, Enterococcus faecium, Lactobacillus acidophilus, L. fermentum, L. lactis, L. plantarum, L. casei, L. brevis, Streptococcus faecium, Clostridium butyricum, Saccharomyces cerevisiae, Aspergillus niger 및 A. oryzae 등이 사용되었다. 냄새제거 효능 평가에서 암모니아 발생 억제 효능을 가진 제품은 전체 제품의 71%이었으며, 48시간까지 효능을 발휘하는 제품은 29%이었다. 암모니아를 30%이상 감소시킨 제품은 암모니아 저감 효능을 나타낸 제품 중 44%를 차지하였고, 24%의 제품이 10% 미만 그리고 나머지 32%의 제품은 10-30% 수준의 저감 효 과를 나타냈다. 황화수소를 30%이상 감소시킨 제품이 32%로 가장 많았고, 8%의 제품이 10% 미만의 그리고 나머지 6%의 제품은 10-30% 수준의 저감 효과를 나타내었다. 이상의 연구 결과, 가축 분뇨의 냄새 방지와 관련하여 다양한 종류의 제제가 국내에 유통되고 있었으며, 앞으로 환경개선제의 효율적 활용 및 관리를 위해서는 정확한 효능분석이 지속적으로 이루어져야 한다.

This paper is derived from a survey research project investigating consumer attitudes towards organic food as the farm animal welfare. Presents focus group results on consumer perceptions, attitudes and recognition about farm animal welfare. We are aimed to conduct the survey research to evaluate the consumer's perception and demands of the farm animal welfare. We deals with the findings obtained from extensive interviews with a representative samples. The objectives of interviews included 20~60 year-old ages lived in Yongin, Anyang, Gunpo, Ansan, Seosan, Dangjin, Iksan and Jeonju city. Questions focused on how people were recognized the need and policies of farm animal welfare. Questionnaire and technical data are analysed and summarized. As the results of the survey research, the objectives of interviews had poorly understood about the policies of farm animal welfare. Conclusionally, the promotions of farm animal welfare policies were need. The development of farm animal welfare policies were desirable for the establishment of animal welfare.

A sundry system is one of popular systems for composting livestock manure, of which main honest is to utilize unlimited, clean, and free solar radiation. A sundry system with a composter of two horizontal screw-type concrete ducts at different height, was constructed and operated for three days for each test in May, 1993, to evaluate its composting performance. Four treatments of the mixture ratio of swine manure and saw dust (manure : sawdust= 1 : 1.25, 1 : 1, 1 : 0.7, 1 : 0.5) were implemented to evaluate the effect of the mixture ratio on degradation of the composting materials of a sundry system with a screw-type composter. Maximum temperature of the composting materials was over 50℃ at D1 or D2 (one or two days after operation starts) for each test. Mean C/N ratio and water contents of the materials were reduced by more than 15 and 20％, respectively. Microbial density of each test showed a typical variation with the lapse of the composting time. Mesophilic microorganism seemed to play more important role on degradation of the materials than thermophilic. A sundry system with a screw-type composter can be considered as a feasible system on basis of maturity data. The conclusion was completely reverse from that of Choi et at., although both adopted a sundry system. A further study is recommended to pursue the cause of better performance of the screw-type composter, whether it was due to affirmative weather or more efficient composter.

본 연구는 안전한 축산물의 생산에 기반이 되는 농가 HACCP 제도 도입에 있어서 일부 문제로 인하여 발생되고 있는 HACCP 인증(재인증) 부적합농가 수의 지속적인 증가에 대 한 문제점을 개선하기 위한 방안으로 최근 3년간 HACCP 심사 결과에 따른 지적비율을 축 종별로 분석하고 상위 10% 이상 지속적으로 지적되고 있는 평가항목을 도출하여 이를 개 선할 수 있는 방안을 마련하고자 실시하였다. 총 15개 HACCP 관리 분야 평가항목 중 지적 비율이 높은 평가항목으로는 소, 돼지 농가에서 4번(가축사육 공정도 및 농장평면도), 10번 (중요관리점 모니터링), 12번(HACCP 검증), 14번(HACCP 교육) 그리고 닭농가에서는 10번 (중요관리점 모니터링), 12번(HACCP 검증), 14번(HACCP 교육)으로 매우 유사한 경향을 나 타내었다. 이러한 문제점을 개선하기 위해서는 농가 HACCP 평가기준의 대폭 축소 및 단 순화, 연 1회 의무 교육 시 해당 분야 집중 교육과 위해요소중점관리기준, 모니터링, 검증 등과 같은 어려운 HACCP 용어를 각각 안전관리기준, 감시, 확인 등으로 이해하기 쉽게 풀 어서 사용하여 국내 축산농가의 현실에 눈높이를 맞추어 개정되어야 많은 축산농가에서 가축사육단계 HACCP 제도를 부담 없이 안정적이고 지속적으로 운용할 수 있을 것으로 판 단된다.