PURPOSES: The object of this study is to select appropriate inorganic materials, and find the best mixing formula to secure fast curing time and enough initial strength, and then to evaluate the durability of the asphalt mixtures according to the degree of addition of the compound manufactured by the determined blending ratio. METHODS : The breaking time and reactivity between seven kinds of inorganic minerals, and the selected recycled aggregate and emulsified asphalt were compared to determine the best initial curing strength for the mixtures. Then, three inorganic materials were chosen as the materials that provide good breaking time and reactivity, and the best mixing formula for the three materials was determined. The chemical composition of the compound manufactured using the mixing formula was analyzed by energy dispersive x-ray system method. Finally, indirect tensile strength (ITS) test was performed (for two days) at room temperature to determine the proper amount of additives that will provide the best initial strength. RESULTS: From the results of the reactivity test, the best mixing formula (A:C:G = 60:30:10) for the three selected inorganic materials with short braking time and high reactivity was determined. The four types of cold reclaimed asphalt mixtures for ITS testing were manufactured by adding the inorganic material compounds at 0%, 3%, 5%, and 7%, and the ITS values were measured after two curing days. The ITS values at 5% and 7% were 0.308 MPa and 0.415 MPa, respectively. The results of quality control tests (Marshall stability, porosity, flow value, etc.) at 5% and 7% satisfied the specification criterion for the cold recycled asphalt mixtures. CONCLUSIONS : The selected inorganic materials (A, C, and G) and the best mixing formula (A:C:G = 60:30:10) accelerated the reaction with emulsified asphalt and shortened the curing time. Depending on the inorganic material used, the breaking time and reactivity can be directly related or unrelated. This is because of the chemical compositions of recycled aggregates, infiltrated foreign matter, and chemical reactions between the inorganic materials and other materials. Therefore, it is important to select the proper materials and the best mixing formula when evaluating the characteristics of the practically used materials such as recycled aggregates, inorganic materials, and emulsified asphalt.

The short-grain rice cultivar Choochung with a rough rice moisture content of 21% was air-dried to equilibrate themoisture content to 15, 16, and 17%. After dehulling, brown rice was packaged for storage in laminated paper orLDPE/OPP plastic-film bags (10-kg size). After storage of the packaged rice kernels in plastic and paper bags at10, 15, and 20oC, the quality characteristics of the rice were evaluated weekly for 16 weeks. Serious losses in theinternal moisture content of the kernels were observed during storage in paper bags, regardless of the moisture con-tent of the kernels or the storage temperature. The plastic-film packaging was effective in maintaining the weightof the kernels for all treatments, but severe fat oxidation problems were encountered, especially when the initialmoisture content of the kernel was 17% and/or the storage temperature of the kernels was 20oC. Before two weeks,the fat acidity value of the kernels reached 20mL KOH/100g, a marginal limit for an acceptable taste of cookedrice. Based on this study, the recommended environment for the storage of brown rice for efficient economic storageof the kernels is an initial kernel moisture content of 16%, laminated plastic-film packaging, and a sub-refrigerationtemperature of 15oC.

Paratlanticus ussuriensis enter prolonged diapause at an egg stage. Environmental conditions, such as temperature, can modify the diapause duration at initial diapause. Eggs enter initial diapause at 20℃, but continued early embryonic development at 30℃. Final diapause at a fully developed embryonic stage is obligatory regardless of temperature conditions. To determine temperature effects on diapause mechanism of P. ussuriensis eggs, we compared weights, DNA and RNA amounts of eggs incubated at either 20℃ or 30℃ for 50 days after oviposition. Both egg weight and total amount of DNA were constant at 20℃ but gradually increased at 30℃. However, total RNA level was rapidly increased at 15 days-old eggs at 30℃ and maintained high levels during further period whereas its level was constant at 20℃. In addition, we identified three heat shock protein 70 (hsp70a, hap70b, hsp70c) genes of P. ussuriensis and determined those expression levels at different temperature conditions. The levels of hsp70a and hsp70b was not detectable until 20 days after oviposition at both temperature conditions, but highly increased at 50 and 60 days when incubated at 30℃. In contrast, hsp70c level was rapidly peaked at 20 days after oviposition, which is the time of initial diapause entrance. Our results suggest that high temperature breakdown initial diapause and a certain hsp gene, such as hsp70c, may involve in the initial diapause mechanism of P. ussuriensis eggs.

The diapause duration P. ussuriensis eggs is known to either one year or prolonged to additional years. This complex life cycle is mainly caused by the interruption at two embryonic stages: early stage (initial diapause) and fully developed late stage (final diapause). The environmental cues, such as temperature, is critical to determine the diapause duration but the precise diapause mechanism of P. ussuriensis eggs is unclear. We demonstrated temperature effects on the entrance of initial diapause. Newly oviposited eggs were incubated at two temperature conditions (20℃and 30℃) for 60 days. When eggs were incubated at 20℃, egg weights were not significantly changed, but at 30℃ those were gradually increased to 1.5 times for 50 days. Genomic DNA contents of eggs were similarly increased at 30℃. Total RNA contents at 30℃ were highly increased from the 15-days-old but not at 20℃. In addition, we further analysed expression levels of two heat shock protein 70 (hsp70a, hap70b) genes during embryonic development. Our results suggest that initial diapause occurred between 10 and 15 days after oviposition and its entrance was blocked by high temperature incubation.

The effects of the initial storage temperature and the PA film packaging on the extension of the shelf-life and the improvement of the postharvest storage quality of muskmelons were studied during their storage. Their storage quality was tested as follows: PA-film-wrapped muskmelons, stored at 2℃ or 7℃ for 30 days after their harvest, were kept at 10℃ for 27 days (total: 57 days). On the fifth day of storage at 10℃ (35th day overall), the weight loss reached 6.4% in the 7-control. However, the 2-PA showed the smallest loss of 2.2%. The soluble solids content and the acidity that were measured before the storage were 10.8 °brix and 0.26% in all the groups. After 27 days of storage at 10℃ (on the 57th day overall), the values were highest in the 2-PA group with 9.7 °brix and 0.15%, respectively. Microorganisms were not detected at first; but on the fifth day of storage at 10℃ (35th day overall), their values were 3.87 and 2.68 log CFU/g in the seven-control and the 2-PA, respectively. In other words, the 2-PA was found to be more effective in inhibiting microbial proliferation. In relation to sensory properties such as appearance, flavor, sweetness and chewiness, the 2-PA was superior to the other groups and was found to be most effective in improving the storability of muskmelons. In conclusion, it was found that low-temperature injury and fast storage quality deterioration did not occur in film-wrapped muskmelons that were stored at 2℃ for 30 days after they were harvested.