This research has attempted to investigate the volatile flavor compounds of onion products through acetic fermentation, and to create a natural beverage with beneficial biological properties which can also fulfill customer quality standards. Onion products (OAF (M): Onion extracts at five days of acetic fermentation, OAF (F): Onion extracts at ten days of acetic fermentation) were produced by acetic fermentation. Volatile flavor compounds from onion extracts, OAF (M) and OAF (F) were used by Mixxor liquid extractions and analyzed by GC/MSD. Compounds of 49, 75 and 69 were identified in onion extracts, OAF(M) and OAF(F) respectively. Among the major volatile flavor compounds classes, sulfur containing compounds (36.7%), acids (31.2%) and aldehydes (13.5%) in onion extracts were changed into acids (69.6%) and alcohols (24.6%) in OAF (M) and acids (80.6%) and alcohols (15.5%) in OAF (F). During acetic fermentation acetic acid, 1,3-butanediol (odorless) and 2,3-butanediol (onion flavor) increased remarkably, sulfur-containing compound such as 2,5-dimethylthiophene having anti-oxidant activities was detected by fermentation.
본 연구는 양파 재배 중 양파와 쇠비름 수액 처리가 무처리구와 비교하여 양파의 수량 및 품질 특성 에 미치는 영향을 분석하고자 하였다. 초장, 엽수 및 엽초경은 양파 생육 전반에서 양파와 쇠비름 추출 액 혼용 처리구가 양파 추출액 처리구와 무처리구에 비해 생육이 양호하였다. 양파 수량은 양파와 쇠비 름 추출액 혼용 처리구가 양파 추출액 처리구 보다 10a당 4.4% 증가 되었으나 유의성이 없었고, 무처 리구 보다는 14.3% 증가되어 유의성을 나타내었다(p<0.05). 양파의 경도는 양파와 쇠비름 추출액 혼용 처리구가 양파 추출액 처리구와 무처리구에 비해 각각 15%와 20% 높아 유의성을 나타내었으나 (p<0.05), 당도는 양파 추출액 처리구에서 양파와 쇠비름 추출액 혼용 처리구와 무처리구 보다 3% 증 가되어 유의성이 나타나지 않았다. 양파의 무기성분 함량은 양파와 쇠비름 추출액 혼용 처리구 양파에 서 K2O와 CaO 제외한 다량원소들이 높게 함유되었다. 양파 생육 시험 후 토양의 무기성분 함량은 양 파와 쇠비름 추출액 혼용 처리구에서 유효인산, 칼륨, 철, 망간, 아연, 구리에서 높게 나타났다.
Total phenol contents, total flavonoid contents and antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from onion (Allium cepa L.) peels were studied. The IC50 values of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity in 70% ethanol extract were remained to be lowest followed by 70% methanol extract and CM extract. And the total phenol content (113.56±0.86 mg CAE/g), total flavonoid content (49.63 mg QE/g) and ferric reducing antioxidant power value were also found to be the highest. In contrast, 70% methanol extract possessed the strongest antioxidant activity by β-carotene bleaching assay. CM extract displayed the lowest antioxidant activity compared with other extracts. Onion peels exhibited strong antioxidant activity and abundant phytochemicals, which could be used in a various food products to add phytochemicals and promote good health.
Onion (Allium cepa L.) contains high levels of dietary fibers and antioxidants, including vitamin C, D, and folates. Onion is also known as a quercetin-rich vegetable with high flavonoid content. Onion peel contains over 20 times more quercetin than onion flesh. The aim of this study was to examine the question of whether onion peel extract supplementation has an effect on maximal exercise performance in rat. Onion peel extracts were extracted with hot water. Thirty male Sprague Dawley rats were maintained on a pellet diet for one week, and then randomly divided into five groups: Normal control, Positive control (quercetin 20 mg/kg), Onion peel 4 mg/kg, Onion peel 20 mg/kg, and Onion peel 100 mg/kg. Oral administration was performed daily. The experimental period was four weeks. Thereafter, animals were then forced to swim in water and the maximal exercise performance period from the swimming start time to the exhausted time, in which they failed to rise to the surface of the water to breathe within a 7 second period, was measured. After necropsy, weights of gastrocnemius muscles were measured. Lactate dehydrogenase concentration in serum was measured using an enzymatic method, using a commercial kit. The maximal exercise performance period was significantly longer in the onion peel extracts fed groups, compared with the control group. The lactate dehydrogenase concentration of the onion peel extracts fed groups was significantly lower, compared with the control group. Based on these results, we suggest that onion peel water extract supplementation can enhance exercise capacity caused by the mechanism of decreasing lactate dehydrogenase concentration.
To control Thrips tabaci in Korean leek and green onion which grow at vinyl house in IKSAN, Jeollabuk-do, the non-treatment was set as negative control. In general treatment group, 120 mesh gauze was installed on the side window after planting(May, 2nd) and, in spring, Orius strigicollis (1.0/m2) was grazed 3 times every 7 days from mid of May which is early development stage and In the mid of June, which is the peak stage, plant extracts were sprayed one time. In autumn, O. strigicollis was grazed 3 times every 7 days from mid of September which is early development stage, and in the mid of October, which is the peak stage, plant extracts were sprayed one time. The result shows that the leaf damage ratio was decreased by 22% in Korean leek and by 27% in green onion compared to the untreatment. And the control value of Thrips tabaci shows 78.7% in korean leak and 90.6% in green onion. The density control effect of Thrips tabaci was significantly controlled under max 6.2/plant in general treatment group compared max 25/plant in the untreatment and this result was similar in green onion. The yields by general treatment was increased by 85% in Korean leek and 56% in green onion, compared with non-treatment yields, which was 900kg/10a in Korean leek and 1,287kg/10a in green onion.
추출방법에 따른 양파껍질 추출물의 품질 특성 및 생리활성 특성을 비교하였다. 환류냉각추출의 수율이 13.21%로 가장 높았고, 폴리페놀과 플라보노이드 함량은 가압가 열추출이 가장 높게 나타났다. DPPH 라디칼 소거능 및 ABTS 라디칼 소거능 또한 가압가열추출이 가장 높게 측정되었다. α-Glucosidase 저해 효과는 80.24%로 환류냉각추출이 가장 높은 저해 효과를 나타내었고, ACE 저해 효과는 27.05%로 저온고압추출이 가장 높았다. 아질산염 소거활성은 가압가열추출이 33.97%로 가장 높았고 다음으로 환류냉각추출이 32.47%로 높은 활성을 나타내었다. Ferrous ionchelating 효과에서는 저온고압추출이 54.73%로 가장 높은활성을 나타내었다. 이러한 결과를 종합해 볼 때 가압가열추출이 소재 활용가치가 높을 것으로 사료되며 천연 항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.
흑양파를 ethanol, methanol 및 물로 각각의 추출물을 제조하고, 이 추출물의 항산화활성을 비교 검토함으로서 흑양파 추출물을 이용한 다양한 가공식품의 제조에 필요한 기초자료를 제공하고자 하였다. 흑양파 물 추출물이 28.56%로 수율이 가장 높았으며, 총 페놀도 13.5 mg/g로 가장 많았다. MTT assay 이용한 Raw 264.7의 세포독성에서 물 추출물이 가장 양호한 세포보호 효과를 보였다. 흑양파 ethanol 추출물과 물
본 연구의 목적은 양파추출물의 간보호 및 항산화 효과를 조사하기 위함이다. 간 손상을 유발시키는 t-BHP (1.5 mM) 존재하에 간세포를 0, 0.01, 0.05, 0.1 및 0.3 mg/ml의 다양한 농도의 양파추출물로 1시간 동안 일차배양하였다. t-BHP는 GOT와 LDH 활성 및 TBARS 농도를 증가시켰으며 MTT값은 감소시켰다. 0.05 mg/ml 농도의 양파추출물 첨가는 t-BHP에 의해 증가된 GOT 및 LDH 활성을 감소시켰으며 0.1 mg/ml 농도의 양파추출물은 t-BHP에 의해 감소된 MTT 값을 증가시켰다. 또한 0.01 mg/ml 농도의 양파추출물 첨가는 t-BHP에 의해 증가된 TBARS 농도를 감소시켜 양파추출물이 t-BHP에 의해 유발된 간손상과 지질과산화를 억제시켰다. t-BHP 처리는 간세포의 catalase, GSH-Px 및 GSH-Rd 활성을 현저히 감소시켰다. 그러나 0.1 mg/ml 농도의 양파추출물 첨가는 t-BHP에 의해 감소된 catalase GSH-Px 및 GSH-Rd 활성을 증가시켰으며 특히 catalase 활성은 t-BHP 무첨가군 수준까지 증가시켰다. 또한 hydroxyl radical을 생성하는 Fenton 시약의 존재하에 plasmid DNA를 양파추출물과 함께 배양한 결과 양파추출물은 농도 의존적으로 hydroxyl radical에 의해 유도된 single-strand 절단을 억제하였다. 이상과 같이 간세포 일차배양에서 양파추출물은 t-BHP에 의해 유발된 간독성, 간세포 생존율 감소, 지질과산화를 농도 의존적으로 억제시켰고 또한 t-BHP에 의해 억제된 GSH-Px, GSH-Rd 및 catalase의 활성을 증가시켰다. 이와 같이 양파추출물의 간보호 및 항산화 효과는 항산화 효소 특히 catalase의 활성 증가와 hydroxyl radical에 의해 유도된 산화억제 및 이에 따른 지질과산화 억제에 기인하는 것으로 사료된다.