Due to the concerns over their environmental and health impacts, there have been attempts for shift towards biorational pesticides from synthetic pesticides. Among them, plant essential oils have emerged as promising active ingredients. Due to the complex interactions among their constituents, the bioactivities of essential oils can vary depending on the compositions, which often undermine their stability in efficacy. Here, we present a model-based optimization approach to develop reliable rosemary oil-based biorational pesticide, against two-spotted spider mites, Tetranychus urticae Koch. The ecotoxicity against Daphnia magna and foliar phytotoxicity against Phaseolus vulgaris were also evaluated. Our quadratic models accurately predicted miticidal activity, ecotoxicity, and phytotoxicity. We aimed to maximize, minimize, and minimize these parameters, respectively. We employed seven multi-objective evolutionary algorithms in Matlab. Among them, the nondominated sorting genetic algorithm II with adaptive rotation based simulated binary crossover (NSGA-II-ARSBX) performed best. We experimentally determined the thresholds for miticidal activity and phytotoxicity, based on the current approval process for agricultural pesticide products in Korea. After applying the thresholds, we validated the obtained viable solutions. Our study offers a novel framework to enhance the reliable and responsible use of essential oils as biorational pesticides.

본 연구는 제주산 자생 로즈마리 에센셜 오일의 항염 및 피부상재균에 대한 항균 활성을 확인하기 위한 것이다. 로즈마리 에센셜 오일은 물 증류법으로 추출하였다. 로즈마리 에센셜 오일의 항염 효능을 확인하기 위하여 RAW 264.7 세포에서 LPS에 의해 유도된 NO와 PGE2의 생성을 농도 의존적으로 억제하는 것을 확인하였다. Western blot 실험을 통해 이들을 생합성하는 효소인 iNOS, COX-2 단백질의 발현이 농도 의존적으로 감소하는 것을 확인하였다. 또한 전염증성 cytokine인 TNF-⍺와 IL-6의 생성 억제 효능을 확인하였다. 항생제 내성균주 각 2종을 포함한 S. epidermidis 3종, P. acnes 3종에 대한 항균 활성을 실험한 결과, paper disc법에서 저해환이 관찰되었고, MIC, MBC 실험을 통하여 균생육 저해와 사멸를 확인하였다. 위의 실험 결과로부터 로즈마리 에센셜 오일의 항염증, 항균 효능을 확인 하였으며 향후 화장품 및 스킨케어 소재로서 활용 가능성을 확인하였다.

The penetration-enhancing effect of a binary mixture of rosemary essential oil constituents, and its correlation to increased toxicity were investigated. GC-MS analyses revealed that the major constituents of rosemary essential oil were 1,8-cineole and camphor, and a binary mixture of them showed synergistic insecticidal activity via topical application in third instar larvae of the cabbage looper, Trichoplusia ni. In the mixture, penetration of camphor was significantly enhanced both in vivo and in vitro, whereas that of 1,8-cineole did not change much compared to individual application. Injection into hemocoel of fifth instar larvae indicated that camphor was more toxic than 1,8-cineole, although it was less toxic than 1,8-cineole when applied topically. A possible mechanism for enhanced penetration of camphor by 1,8-cineole is reduced surface tension of the mixture. Contact angles of 50% acetonic solutions on a layer of beeswax showed that the surface tension of 1,8-cineole was lower than that of camphor. Another probable reason is a change of camphor from solid to liquid status, when mixed with 1,8-cineole. This is the first report attempting to explain the synergistic insecticidal interaction of essential oil components, from a physiochemical perspective.

Quality stability of the herb pill coated with edible oils containing rosemary was investigated. Herb pills were made of herb powders such as Panax ginseng, Cinnamomum cassia, Lycium chinense, Zyzyphus jujuba and Zingiber officinale. Rapeseed oil and lubriol were used as edible coating oil. After herb pills coated with edible oils with or without rosemary were stored at 40℃ for 180 days, the microbial viable cell counts and peroxide values(POV) of the herb pill were investigated. After 180 day storage, POVs of herb pills with only rapeseed oil or lubriol were 0.51 and 0.49 meq/kg, respectively. However, when rosemary was added in herb pills the POVs were decreased to 0.30 and 0.39 meq/kg, respectively. The addition of rosemary to the rapeseed oil and lubriol tended to decrease the microbial viable cell counts of the herb pill. The microbial viable cell counts of rapeseed oil and lubriol were 940 and 820CFU/g, respectively after 180 days of storage. However, these levels were suppressed to 720 and 640CFU/g by the resemary addition. On the other hand, the ginseng saponin content of herb pills was not affected by the rosemary addition during storage.

The purpose of this study was to evaluate the effect of essential oil on wound healing rate, an epithelial attachment and collagen density in wound induced rat. This study was a 2×3 factorial design. Factors were treatment period (4 day and 8 day) and treatment type [saline (0.9% NaCl), tyrothricin, mixture of tea-tree, rosemary and melissa oil]. The samples were 24 rats with wound and randomly assigned to six groups of four rats per group. The dependent variables such as a wound healing rate, an epithelial attachment and a collagen density were measured. Dependent variables were statistically significant difference by the two treatment periods and the three treatment types (p<.001). There were not statistically significant interactions between treatment periods and treatment type in all dependent variables (p>.05). These findings will be provide bases to develope effective interventions for patients who have wound to manage their wound.