This study was carried out to examine the effective wet harvesting solution for development of wet distribution system in standard chrysanthemum (Dendranthema grandiflorum) ‘Baekma’. The cut flowers were treated immediately in floral preservative solutions or dry condition after harvesting, and then the effects on quality of cut flower were compared. Also, we investigated the effects of NaOCl and sucrose on vase life and quality of cut flower. When the cut flowers were treated immediately in tap water, Chrysal OVB, Floralife, Hiflora solutions after harvesting, flower diameter and fresh weight of cut flower increased compared to dry condition treatment. In single treatment of 100 mg･L-1 NaOCl as wet harvesting solution, flower diameter and fresh weight of cut flower increased more than other treatments, and vase life was prolonged to 1.5 days than control. But, flower diameter and fresh weight of cut flower decreased in 0 or 200 mg･L-1 NaOCl. When the cut flowers were treated in combination solution of 100 mg･L-1 NaOCl and 0.1% sucrose, the flower diameter was the largest by 9.8 cm, and fresh weight of cut flower was maintained the highest in holding solution. On the other hand, flower diameter and fresh weight of cut flower were lowest in combination solution of 100 mg･L-1 NaOCl and 2.5% sucrose. There was no difference in vase life between treatments mixed with NaOCl and sucrose. Therefore, it was suggested that treatment mixed with 100 mg･L-1 NaOCl and 0.1% sucrose as wet harvesting solution was the most effective for vase life and quality of cut flower in standard chrysanthemum ‘Baekma’.

This study was carried out to examine the effects of NaOCl, sucrose, and BA concentration as pretreatment solution on quality and vase life of cut flowers in Dendranthema grandiflorum ‘Jinba’. Flower diameter, fresh weight, and vase life in control and 0 mg･L -1 NaOCl treatments decreased, and the treatment with 100~200 mg･L -1 NaOCl was more effective in the quality and vase life. In pretreatment with 2.5% sucrose solution, flower diameter and fresh weight decreased and vase life was shortest due to the rapid leaf wilting. However, pretreatment with 0.1% sucrose solution increased the flower diameter and fresh weight, and showed the longest vase life. When more than 80 mg･L -1 BA was treated with pretreatment solution, flower diameter and fresh weight decreased, and vase life was shortened. With pretreatment of 20 mg･L -1 BA, the flower diameter was bigger than in the other treatments, but it was no effect on fresh weight and vase life. Therefore, it was suggested that pretreatment solution mixed with 200 mg･L -1 NaOCl, 0.1% sucrose, and 20 mg･L -1 BA was the most effective for the quality and vase life of cut flowers in standard chrysanthemum ‘Jinba’.

This study was carried out to investigate the effect of sucrose, boric acid, and calcium nitrate in optimum pH and relative humidity on pollen germination and pollen tube growth of boxthorn (Lycium chinense M.). Medium containing 100mg/L of H3BO3 showed maximum pollen germination and pollen tube elongation, 64.8% and 920μm. Additionally supplied calcium nitrate to the medium lowered pollen germination rate and pollen tube elongation. Here we report the optimum conditions for the pollen germination and pollen tube development was observed at 10% sucrose, 1.0% agar, pH 6.0, and 100% relative humidity.

Sucrose, raffinose, and stachyose are major soluble sugars in soybean seeds. Sucrose is the major source of energy for fermentation and contributes to the sweetness of soybean foods. The soyfood and animal feed markets prefer soybean cultivars with high sucrose because they provide high levels of energy and better tasting food and feed. The objective of this study is to select soybean lines with high sucrose content. A total of 295 soybean genotypes including 13 current cultivars, 34 germplasms, and 248 breeding lines were planted at the field. After harvesting, sucrose contents for 295 genotypes were measured through HPLC method. Wide variation was detected among the 295 genotypes in sucrose content. The sucrose content ranged from 15.1 to 39.0 g. kg-1 in 13 current cultivars. The highest sucrose content was identified in “Dayangkong” (39.0 g. kg-1), whereas was lowest in “Seomoktae” (15.1 g. kg-1). The sucrose content ranged from 9.6 to 47.6 g. kg-1 in 34 germplasms. The sucrose content ranged from 0.9 to 46.4 g. kg-1 in 248 breeding lines.

Effects of nitrogen (N), phosphorous (P) and potassium (K) on the shoot and bulb growth of wild garlic (Allium victorialis var. platyphyllum) were studied by adopting in vitro culture. These macronutrients influenced the growth of both the shoot and bulb of garlic depending upon their application doses. A minimum of 3% potassium nitrate (KNO3) as a source of nitrogen was found to be critical for shoot elongation while higher concentrations were inhibitory. Garlic bulb growth was profuse on the usual KNO3 strength and sucrose (7%), followed by KNO3 (9.4 mM) supplement. On providing 41.22 mM ammonium nitrate (NH4NO3) as nitrogen source highest shoot growth was observed while 82.45 mM NH4NO3 as a source of nitrogen supported high bulb growth. With regard to potassium a good shoot growth was observed in medium that contained 0.31 mM KH2PO4 and 3% sucrose, while bulb growth was high on 2.5 mM KH2PO4 and 7% sucrose. These experiments may thus direct the development of excellent growth conditions for the commercial production of edible wild garlic.

The rice sucrose synthase 3 (RSUS3) localized predominantly inrice seed endosperm may play an important role in the starch filling in the milky stage of rice seed. As the genetic diversity at this locus is not known yet, forty three rice varieties/accessions were objected to amplify full sequence of the RSUS3 to examine the distribution of DNA polymorphisms. A total of 254 sequence variants, including 82, 150 and 22SNP sand indels, were successfully identified in whole length of 7,733bp sequence comprising promoter, exon and intron, and 3’ down stream non transcribed region(NTR). Eleven haplotypes were distinguishable among 43 rice varieties based on the nucleotide variation on the three defined regions (5’NTR, transcript and 3’NTR). The promoter region showed the occurrence of a base change on a cis-element which might involve a functional role of the motif in seed-specific expression. Non random process seemed to be acted in the genetic diversity of RSUS3geneamongricegermplasmusedinthisstudy. The analysis of polymorphism sites indicated a history of eleven minimum recombination mostly occurred in the transcribed region. This result might provide an insight for a clasditic approach for establishing future genetic association studies of RSUS3locus.

절화의 관상기간 중 꽃잎의 탈색 완화에 적합한 조건을 알아보기 위하여 리시안서스, 용담, 국화, 공작초를 대상으로 광도(500, 5,000, 10,000lux)와 자당 처리를 실시하였다. 광도의 차이와 자당의 유무의 차이를 비교해 볼 때 리시안서스와 용담은 각각 자당이 처리된 5,000lux에서 10.9일 10,000lux 역시 10.9일로 관상기관이 길게 나왔다. 반면 국화는 500lux에서 10.5일로 관상기관이 길게 나왔다. 공작초는 광도와 상관없이 대조구에서 10.2일로 관상기간이 길었다. 꽃잎의 탈색완화 효과는 가장 높은 광도 하의 꽃잎 색이 진하게 나왔고, 대조구 보다는 자당이 첨가된 처리구에서 진하게 나오는것을 알 수 있었다. 이상의 결과로부터 고광도의 조건과 자당의 첨가는 안토시아닌의 합성을 증가시키기 때문에, 꽃잎탈색을 지연시키는 것으로 나타났다.

Sucrose synthase 3 which is a third active gene present in rice, is localized predominantly in rice endosperm. This sucrose synthase 3 may play an important role in the starch filling in the milky stage rice seed, probably involving in the starch physicochemical properties. As the genetic diversity at this locus is little informed, forty three rice consisting of japonica, indica and Oryza rufipogon were targeted to amplify full sequence of sucrose synthase 3 to examine the frequency and distribution of nucleotide polymorphism. Total of 755 all sequence variants detected, 491 single nucleotide polymorphisms (SNPs) and 264 indels were successfully identified in 7618 bp of sequence containing the sucrose synthase 3 transcript, promoter and 3' non-transcribed region. The frequency of nucleotide changes and indels were high, on average one polymorphism per 15.5 bp and one indel per 28.9 bp with 11 sequence-based haplotypes distinguishable among the varieties and lines. Both the frequency of nucleotide changes and indels were frequent in non-coding region, but rare in coding region. Sequencing a polymorphism region in the promoter showed one base change on one of cis-element from T (CATGCATA to A (CATGCACA) that might implicate in seed specificity. The presence of a high number of haplotype shared by a few varieties indicated a little information on linkage disequilibrium.

The content of sucrose is one of the most important characters in the value of quality of melon fruit. For genetic studies of sucrose content, a wild melon GM42, as a very low sucrose parent (mean, 9.82 mg · g^-1 fresh wt) was crossed with a hig