In order to investigate the optimal UVB (ultraviolet B) treatment conditions for vitamin D2 enhancement of freeze-dried ear mushroom, sample size (below 300 μm~whole), UV treatment temperature (30~60℃), treatment density (6.25~50.0 mg/cm2) and the samples mixing frequency (1~32 times) were treated differently. After that, chromaticity, vitamin D2 and ergosterol (vitamin D2 precursor) contents were investigated. As a result of the investigating, effective UVB treatment conditions for vitamin D2 enhancement are as follows. The sample sizes were 2~4 mm and finely crushed pieces. The treatment temperatures were 50℃ and 60℃. The treatment density was 12.5 to 25.0 mg/cm2, and the number of sample mixing was 8 times or more. As the amount of vitamin D2 increased by UVB treatment, the ergosterol content generally tended to decrease. However, under some UVB treatment conditions, the vitamin D2 content was not high despite the decrease of ergosterol content. Under the conditions set in this experiment, it was possible to obtain ear mushrooms with enhanced vitamin D2 up to 26,968.7 μg/100 g. Therefore, it is thought that the ear mushroom is highly likely to be used as a vitamin D source and nutritionally fortified food ingredient.

Repetitive or excessive exposure to ultraviolet (UV) radiation causes oxidative stress-mediated skin photoaging through the overproduction of reactive oxygen species. Actinidia polygama is known as a medical plant used in oriental medicine for treating several diseases such as abdominal pain, stroke and rheumatoid arthritis. Recently, it was reported that A. polygama extract had anti-wrinkle and skin hydrating properties in ultraviolet B (UVB)-exposed hairless mice. However, the molecular biological mechanism of this extract on alleviating skin photoaging is still unknown. Therefore, we investigated the anti-photoaging effects of PB203, which is the powder of A. polygama extract, in the in vivo and in vitro photoaging models. First, PB203 showed 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities due to the presence of anti-oxidant components including flavonoids and polyphenols. In UVB-irradiated hairless mice, oral administration of PB203 (100 mg/ kg) significantly improved wrinkle formation, skin dehydration, elasticity and skin barrier function by decreasing the levels of matrix metalloproteinases (MMPs) and increasing those of collagen I, filaggrin, involucrin and loricrin. Especially, the reduced production of p-p38, p-c-Jun and p-c-Fos by PB203 reversed the elevated levels of MMPs mediated by UVB exposure, resulting in the upregulation of collagen I expression. Consistent with these animal data, PB203 remarkably enhanced the mRNA expression of collagen I, filaggrin, involucrin and loricrin, while suppressed that of MMPs in UVB-irradiated HaCaT cells. And PB203 increased the wound recovery rate of cells by promoting their proliferation and migration. Moreover, PB203 significantly recovered the activity of superoxide dismutase inhibited by UVB in both mice and cells. In conclusion, PB203, which protects skin from UVB-induced photodamage by exerting antioxidant properties, can be considered to have sufficient potential as a functional ingredient or therapeutic agent improving skin photoaging and related skin symptoms.

This study was conducted to effectively supplement vitamin D and increase the consumption of ear mushroom based on the investigation of the quality characteristics of cookies containing ear mushroom supplemented with vitamin D. Cookies were made of 0%, 1%, 3%, and 5% ear mushroom powder treated by ultraviolet B. Increased addition of ear mushroom powder led to a decrease in the bulk density of the dough as well as a spread factor, color value, and hardness of the cookies. There was no significant difference in the loss rate, but the water content of the dough and cookies was increased. The total polyphenol content of cookies added with 1~5% ear mushroom powder was higher than that of the 0%. The DPPH free radical scavenging activity of cookies added with 5% ear mushroom powder (23.8%) was 2 times higher than that of the 0% (10.9%). The vitamin D2 content of cookies added with 5% ear mushroom powder (835.5 μg/100 g D.W.) was 44 times higher than that of the 0% (19.0 μg/100 g D.W.). Consequently, ear mushroom powder is considered to be suitable for the production of functional cookies because of high values of antioxidant activity and vitamin D2 content.

To examine the possibility of ear mushroom (EM) as a source of natural vitamin D, the UVB (ultraviolet B) was treated according to sample drying status, drying methods before UVB treatment and harvest time. And then, vitamin D2 and ergosterol contents were investigated. According to the sample drying status, the vitamin D2 contents of fresh and freeze-dried EM (whole) increased to 4,634.4~4,780.9 μg/100 g D.W. (dry weight) under UVB dose 52.5~70.0 kJ/m2 and above 18,693.1 μg/100 g D.W. under above 105 kJ/m2, respectively. By drying methods before UVB treatment, vitamin D2 contents of EM powder (below 500 μm) that dried in the vinyl house and freeze-dryer increased to 4,886.2~5,132.9 μg/100 g D.W. under above 105 kJ/m2 and 17,103.7 μg/100 g D.W. under 70 kJ/m2, respectively. Ergosterol content decreased with increasing UVB dose in all experiments. According to the harvest time, vitamin D2 content under UVB dose 210 kJ/m2 showed marked difference and in order of June, July, August, October and April. As for the results, the optimum harvest time, drying method before UVB treatment, sample size, UVB dose for the EM contained high vitamin D2 content were June, freeze-drying, whole, and 105 kJ/m2, respectively.

Low molecular weight hydrolysates from donkey bone extracts (LHDB) was prepared with different food enzymes, and its antioxidative, elastase and collagenase inhibitory, and fibroblast cell protection effects against photoaging were evaluated. Gelatin from donkey bone was extracted three times at 121℃ for 1 h and was lyophilized. The lyophilized powder (5 g) was dissolved in 95 mL distilled water with 1% FoodPro alkaline protease (A), 1% Protease P (P), 1% Protease M (M) and a 0.3% A + 0.3% P + 0.3% M (APM) mixture and was hydrolyzed for 3 h at 45℃. After enzyme inactivation at 90℃ for 10 min, the LHDBs hydrolyzed by A, P, M, and APM were separated by centrifugal filtration and were lyophilized and marked as LHDB-A, LHDB-P, LHDB-M, LHDB-APM. The LHDB-M showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline–6- sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) than the other treatments (p<0.05). The elastase inhibition effect (37.49%) of LHDB-M were significantly higher than those of the other treatments (9.97-34.18%). The viability of human fibroblast cells (Hs68) after UVB irradiation was significantly increased by LHDB-M, indicating that it can be used as an antioxidant or as a UVB stress protector. However, further in vivo studies should precede its usage in the bioactive compound industry.

Terrestrial animals are widely considered to be well protected from damage caused by ambient UV radiation, and thus the direct effects of solar UV-B radiation on arthropods have not attracted a great deal of attention. However, if plant-dwelling arthropods have evolved behavioral adaptations to solar UV-B radiation, but not morphological or physiological adaptations, the resources available to them would be strongly limited. Tetranychus urticae and their dominant natural enemies, phytoseiid mites, usually stay on the lower leaf surfaces. Because of the accumulation of UV-B-absorbing compounds such as flavonoids in the leaves, the upper and lower leaf surfaces provide different UV environments. We tested whether UV irradiation affects survival and reproduction of T. urticae and, if so, whether staying on the lower leaf surfaces is beneficial for their performance in solar UV radiation. Consequently, we observed lethal effects of artificial UV-B irradiation and solar UV radiation on T. urticae. However, by remaining on lower leaf surfaces, the mites could avoid such deleterious effects. These suggest that staying on the lower leaf surfaces is a behavioral adaptation of T. urticae to avoiding harmful solar UV-B radiation. Lethal effects of UV-B irradiation also exerted strong deterrent effects on the hatchability of phytoseiid mites. Additionally, preliminary experiments suggested that phytoseiid mites avoided and quickly escaped from a place irradiated by solar UV. Therefore, solar UV-B radiation may strongly affect the diurnal and spatial patterns of distribution or migration of mite communities on host plant canopies.

This study was to investigate the effect of ultraviolet rays, soaking, boiling and baking to ergocalciferol (Vit.D2) and riboflavin(Vit.B2) contents of mushrooms, Lentinus edodes, Pleurotus ostreatus and Agaricus bisporus. The results were as follow: 1. Mushrooms were exposed to ultraviolet rays Lentinus edodes : 10J/cm2, Pleurotus ostratus : 2J/cm2 and Agaricus bisporus : 2J/cm2. 2. Before exposing to ultraviolet rays, the ergocalciferol contents of mushrooms were all 0μg/g dry base, but after exposing to it , those of Lentinus edodes, Pleurotus ostreatus and Agaricus bisporus were 222.50±5.30μg/g dry base, 150.90±6.60μg/g dry base and 23.98±1.20μg/g dry base, respectively 3. Before and after exposing to ultraviolet rays, the riboflavin contents of Lentinus edoes, Pleurotus ostreatus and Agaricus bisporus were 18.22±0.71μg/g dry base and 11.72±0.50μg/g dry base, 4.57±0.20μg/g dery base and 3.26±0.15μg/g dry base, and 37.42±1.20μg/g dry base and 27.33±2.10μg/g dry base, respectively. 4. The ergocalciferol contents of mushrooms according to boiling time were not significantly different but the riboflavin contents of them were decreased according to the increase of boiling time. 5. The ergocalciferol and riboflavin contents of mushrooms were decreased according to the increase of NaCl concentration and baking temperature. 6. The ergocalciferol content of Lentinus edodes after a short time soaking at 80℃ was higher than a long time soaking at 20℃, 40℃ and 60℃.

Miliaria crystallina is due to obstruction of the eccrine sweat duct as it courses through the stratum corneum. Miliaria crystallina consists of superficial, subcorneal, and noninflammatory vesicles. Ultraviolet light exposure, resident organisms on skin, and repeated sweating episodes are considered as facilitating factors. Ultraviolet phototherapy with narrowband ultraviolet B (NBUVB) has been known to have a beneficial effect on various skin diseases. It may be considered to be an alternative for generalized pruritus patients, especially if they are resistant to standard treatments. Herein, we present a case of adult miliaria crystallina due to artificial ultraviolet radiation for phototherapy. Although it is underestimated for self-limited disorder and short duration, we should keep in mind that NBUVB phototherapy commonly used in dermatology may cause a miliaria crystallina.

In this study, we investigated the anti oxidative potential and protective effects of water extract of Leonurus sibiricus L. leaf (LSLW) against ultraviolet B (UVB)-induced oxidative damage in human keratinocytes (HaCaT cells). To evaluate the anti oxidative activity of LSLW, we measured DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion scavenging activities, lipid peroxidation inhibition, and reducing power of LSLW. For induction of oxidative stress in HaCaT cells, the cells were irradiated with UVB at 40 mJ/㎠. To investigate the protective effects of LSLW against UVB, we measured cell viability and apoptotic bodies using annexin V staining. LSLW showed anti oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion and by reducing lipid peroxidation. In addition, LSLW showed high reducing values. The UVB-induced oxidative conditions led to cell apoptosis. However, treatment with LSLW ameliorated oxidative stress conditions, including inhibition of cell death, apoptotic body. Taken together, LSLW exhibited anti oxidative and protective effects against UVB-induced damage in HaCaT cells. Thus, LSLW could be useful for the development of cosmetics for UVB-induced skin aging.

피부는 외부 유해물질로부터 내부기관을 보호하는 장벽기능을 하는 대표적인 기관으로 자외선 (ultraviolet radiation, UV), 중금속, 각종 산화 물질들과 같은 외부의 위협에 항상 노출되어 있어 손상을 받 기 쉽다. 특히 자외선 B (UVB)는 진피의 상부까지 도달하여 화상이나 홍반과 같은 염증반응을 일으키며 멜라 닌 생성을 촉진시켜 색소 침착을 유발한다. 지나친 자외선 B의 피부세포로의 유입은 각질세포 및 진피 섬유아 세포의 DNA손상을 야기하고, 세포외기질의 합성을 방해하여 피부탄력감소, 주름생성, 진피 결합조직의 손상 과 함께 피부장벽을 붕괴시켜 노화를 일으키며, 장기간 노출 시 심각한 피부 병변으로 이어져 피부세포 사멸 및 종양의 발생으로까지 이어진다. Haloarcula vallismortis는 사해로부터 분리 동정한 미생물로 호염성 고생 물의 생장적 특징은 아직 자세히 연구된 바는 없다. 대게 10% 이상의 염도에서 자라는데 실제 생장염도는 평균 20 ∼ 25% 염도에서 자란다. 염도가 높은 호수나 염전에서 주로 발견되기 때문에 강한 햇빛에 대한 방 어기작이 존재한다. 그중 하나로 다른 ATP를 생성하기 위한 bacteriorhodopsin외에 halorhodopsin이라는 색소를 이용, 광자(photon)를 흡수하고 염화이온 채널을 개방시켜 생기는 전위차를 이용해 추가로 에너지를 생산한다. 또 carotenoid 색소로 인해 농도가 높을시 분홍색 또는 빨간색을 띄는 특징이 있는데 이것이 강한 자외선에 대한 방어기작을 할 것이라고 여겨진다. 본 연구는 호염성 고세균이 자외선을 에너지 소스로 이용하 는 특성을 이용하여 자외선으로부터 피부를 방어하는 방법을 개발하고자 이들의 피부각질세포에서 자외선에 의한 항염 효과 및 DNA 손상 방어 효과를 확인하였다. 그리고 이들을 천연 자외선 차단제의 소재로서 사용 할 수 있음을 확인하였다.

This study has carried out to evaluate the effect of NaCl as electrolyte of single (electrolysis and UV process) and complex (electrolysis/UV) processes for the purpose of removal and mineralization of Rhodamine B (RhB) dye in water. It also evaluated the synergetic effect on the combination of electrolysis and UV process. The experimental results showed that RhB removal of UV process was decreased with increase of NaCl, while RhB removal of electrolysis and electrolysis/UV process was increased with increase of NaCl. The decolorization rate of the RhB solution in every process was more rapid than the mineralization rate identified by COD removal. The latter took longer time for further oxidation. Absorption spectra of an aqueous solution containing RhB showed a continued diminution of the RhB concentration in the bulk solution: concomitantly, no new absorption peaks appeared. This confirmed the decolorization of RhB, i.e., the breakup of the chromophores. It was observed that RhB removal in electrolysis/UV process is similar to the sum of the UV and electrolysis. However, it was found that the COD of RhB could be degraded more efficiently by the electrolysis/UV process than the sum of the two individual process. A synergetic effect was demonstrated in electrolysis/UV process.

Reductions in the ozone column have led to substantial increase in UV-B radiation at the Earth's surface with the amount and intensity dependent on atmospheric and geographic factors. Our objectives were to understand the effect of UV-B radiation on photosynthesis and carbohydrate synthesis of soybean, and to establish an indicator to select resistant soybean cultivar against UV-B radiation. Soybean seeds (cv. Daepungkong) were sown and grown for 4 weeks in an environmentally controlled chamber. UV-B radiation was delivered to plants for four hours, each day, from 10:00 to 14:00 h by five fluorescent UV lamps. A distance of 1 m from lamps to the top of plants was always maintained throughout the experiment. The biologically effective UV-B radiation level was 21.6 kJ m-2 d-1 using the generalized plant responses action spectrum normalized at 300 nm. The damage of soybean leaves was optically observed at the second day of UV-B radiation, and leaf veins were first injured. Photosynthetic rate was reduced after 2 days of UV-B radiation, and, at 5 days, it was a half level (6.3 μmol m-2 s-1) compared with control (12.1 μmol m-2 s-1). UV-B radiation led to the reduction of stomatal conductance around 5 days of treatment. The decrease in stomatal conductance means the reduction of leaf transpiration and the influx of carbon dioxide. Also, the reduction of photosynthetic rate caused an accumulation of the intercellular carbon dioxide. It is suggested that photosynthetic characteristics can be used an indicator to select UV-B-resistant soybean cultivar.

In the present study we studied the growth, photosynthetic traits and protective mechanisms against oxidative stress in the primary loaves of cucumber (Cucumis sativus L.) seedlings with or without UV-B treatment. Cucumber seedings were irradiated with UV-B for 10 days in environment-controlled growth chambers. The primary leaves irradiated with UV-B showed reduction in leaf length and decreased biomass production. The reduced biomass production seemed to be due to a negative effect of UV-B radiation on the photosynthetic process. Changes in chemical properties of leaf, such as chi a/b ratio affected photosynthesis. UV-B significantly affected chl b content compared with chi a in the light harvesting complex resulting reduced photosynthetic activity Fv/Fm decreased with an UV-B stress, suggesting that the photosynthetic apparatus, and particularly, PS II was damaged under UV-B stress. Malondialdehyde(MDA) concentration which represents the state of membrane lipid peroxidation Increased significantly under UV-B stress confirming an oxidative stress. UV-B exposure with SA solution(0.1-1.0 mM) can partially ameliorated some of the detrimental effects of UV-B stress. Leaf injuries including loss of chlorophyll and decreased ratio of Fv/Fm were reduced with combined application of UV-B and SA. ABA and JA showed similar mode of action in physiological effects on photosynthetic activities though the levels were lower than those from SA treated plants. Chloroplast ultrastructure was also affected by UV-B exposure. The thickness of leaf tissue components decreased and the number of grana and thylakoids was reduced in chloroplast applied UV-B or SA alone. At combined stress granal and stromal thylakoids were less affected. The leaves under combined stress acquired a significant tolerance to oxidative stress. From these results, it can be suggested that SA may have involved a protective role against UV-B induced oxidative damage.