Allicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study was to investigate the effect of allicin treatment during in vitro maturation (IVM) on porcine oocyte maturation and developmental competence. Porcine follicular oocytes were cultured in 0 (control), 0.01, 0.1, 1, 10, and 100 μM AL added IVM media. The rate of polar body emission was higher in the 0.1 μM AL-treated group (74.5% ± 2.3%) than in the control (68.0% ± 2.6%). After parthenogenetic activation, the rates of cleavage and blastocyst formation were significantly higher in the 0.1 μM AL-treated group than in the control (p < 0.05). The reactive oxygen species level at metaphase II was not significantly different among all groups. In matured oocytes, the relative mRNA expression of both BAK and CASP3, and BIRC5 were significantly lower and higher, respectively, in the 0.1 AL-treated group than in the control. Also, the mRNA expression of BMP15 and cyclin B, and the activity of phospho-p44/42 MAPK, was significantly increased. These results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes.

Spindlin1(Spin1), a meiotic spindle-binding protein that is highly expressed in cancer cells. Spindle-binding was dependent on its phosphorylation status, which was partially regulated by Mos/MAP kinase pathway. Nevertheless, the biologic roles of Spin1 in oocytes maturation are still largely unknown. For exploring the function of Spin1 in porcine oocyte maturation, Knockdown and overexpression methods were employed to the present study. Spin1 mRNA were enriched in maternal stages, from germinal vesicle - to 2 cell - stage, but sharply decreased after 4 cell stage, zygotic genome activation. Protein of SPIN1 was localized in spindle-chromatin complex during the metaphase I and metaphase II stages. Knockdown of Spin1 did not affect the first polar body extrusion, however, Spin1 depletion caused mitotic spindle defects, chromosome instability and pronuclear formation in metaphase II stage. Percentage of 2cell, 4cell embryos and blastocyst formation were significantly reduced in knockdown group compared with control, but cell numbers in blastocyst were no difference between control and knockdown groups. Another hand, Oocyte failed to maturation and induced metaphase I arrest following Spin1 over-expression. In conclusion, Spin1 is involved in the spindle formation and maintenance during oocytes meiotic maturation in pigs.

This study was conducted to evaluate the genetic distances and specific DNA makers by the randomly amplified polymorphic DNA (RAPD)-PCR method for the Korean native chickens(red plumage, red-line plumage, Ogol) and white leghorn. Genomic DNA was extracted from plumage from chickens after they were slaughtered. The extracted DNA was observed by nano-spectrometer. RAPD analysis was performed using 13 different primers. Statistical analysis was made for the estimation of the genetic distance among the chicken’s and the cluster tree was drawn by using MEGA 5.05 software. Genetic relations among them were determined by RAPD analysis. The polymorphic bands were observed 72% and the rest of 28% was monomorpic. The largest genetic distance (2.266) was found between the native chickens(red, red-line) and the ogol chickens by UPGMAP method and the closest distance was observed between the ogol in korean chickens as expected. The highest genetic distance between them was estimated 2.266 and in the dendrogram analysis, among I and II within cluster II, most of the ogol chickens were in IIB, indicating the expression of the ogol color could be due to original and the ancestral genetic crossing. Thus, this genetic distance can be useful as the differential genomic information in the normal(red plumage, red-line plumage) and ogol of korean native chickens. This work was supported by a grant from the “Livestock Preservation of Genetic Resources", Rural Development Administration, Republic of Korea.

물장군(Lethocerus deyrollei (Vuillefroy))은 노린재목(Hemiptera) 물장군과(Belostomatidae) 물장군속(Lethocerus)으로 분류하며 국내에서 서식하는 노린재 중 가장 대형 종으로 1998년 환경부에서 지정한 멸종위기 야생생물 II급 보호종이 다. 본 연구는 2016년 6월 8일부터 9월 24일까지 광주기가 인공 부화된 물장군의 생장에 미치는 영향을 평가하기 위해 25℃, 광주기 16L:8D, 12L:12D, 8L:16D 조건에서 105개체를 대상으로 실험을 실시하였다. 조사결과 16L:8D에서는 6.30일(1령), 5.58일(2령), 6.70일(3령), 10.73일(4령), 21.72일(5령)로 총 50.80일이었고, 12L:12D에서는 각 령기별 생장 기간이 5.26일(1령), 4.51일(2령), 5.10(3령), 7.52(4령), 16.73(5령)로 총 38.61일이며, 8L:16D에서는 5.06일(1령), 5.11일(2 령), 6.24일(3령), 7.71일(4령), 15.61일(5령)로 총 38.43일로 나타내어 16L:8D 조건보다 12L:12D조건이 76%, 8L:16D조건 은 75.64%로 생장 기간이 짧은 것으로 판명되었다.

캄보디아와 라오스에는 지금까지 가루깍지벌레과 25종 및 18종이 각각 보고되어 있다. 2015년 이들 지역에서 가루깍지벌레과14종이 채집 되었으며, 그 중 캄보디아에서 2종[Antonina graminis (Maskell, 1897), Coccidohystrix insolita (Green, 1908)], 라오스에서 5종[Nipaecoccus viridis (Newstead, 1894), Paracoccus marginatus Williams & Granara de Willink, 1992, Phenacoccus solani (Ferris, 1918), Rastrococcus iceryoides (Green, 1908)]을 처음으로 보고한다.

This study was conducted to report the census survey results of south Korean Chikso (Korean Brindle Cattle) distribution and to diagnose the current coat color expression patterns. Two years Chikso census showed that there were 2,413 Chikso in 2013 and 2,754 in 2014 in south Korea. Number of animals between 0 to 35 months of age was 1,632 heads in 2014 which comprised 59% of total Chikso population size. The percentages of animals between 36 ~ 71 months of age and over 6 years of age were 31% and 10% of total. Out of 2,416 animals with class numbers assessed by their coat colors, 1,249 heads (52%) were in group I (with black stripes, class no. 1 ~ 3) and the others (class no. 4 ~ 7) were in group II (1,167 heads, 48%). Among the 1,551 animals that were photographed twice in 2013 and in 2014, 226 animals were assessed different coat color class numbers, and round 90% of which were within the ages less than 48 months. The number of animals switched in coat color pattern groups over a year was 56, which was 3.6% of total number of animals on survey. And around 88% of the animals switched in group category was of the animals younger than 24 months of age. Therefore, we conclude that the coat color pattern becomes rather stable at around 24 months of age in Chikso populaion in south Korea.

This research project provides the opportunity of foundation for Ministry of Agriculture y Ganaderia (MAG), University of El Savador (UES), and Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA) and potato farmers of El Salvador to implement to achieve the project goal of poverty alleviation, nutrition improvement, and income increase of farmers through the establishment of sustainable self dependent national infrastructure for seed potato production and supply system and organized network of production of farmers. Kangwon National University has been designated by KOICA to implement the APP Track-II Project from April, 2015 to March, 2016. The support activities and research result include 'virus-free seed potato production facilities and laboratory equipment' provided to UES and CENTA, 'technology transfer of virus free seed potato production' such as making substrate, tissue culture, pathology test, subculture, mini tuber production, and some joint research result achievement, and various technological training conducted for participating researchers and local potato farmers for the virus-free seed potato production, table potato and seed potato cultivation technology, and the standard farming technology as well.

현행 규제요건에 따르면 국내에서 발생된 모든 폐밀봉선원은 자체처분 대상, 극저준위 또는 중·저준위 방사성폐기물에 해 당하며, 기본적으로 방사능 농도를 기준으로 한 처분방식 제한규정을 준수해야 한다. 본 연구에서는 이러한 분류체계 이외 에 IAEA 및 국외 폐밀봉선원 사용국의 방사성폐기물 분류체계, 폐밀봉선원 고유 특성 등에 대한 검토 및 분석결과를 토대 로 반감기 및 A/D 값(각 선원의 방사능(A)을 작업자 및 일반 대중에 대한 잠재적 위험도를 의미하는 방사성핵종 고유의‘D 값’을 활용하여 정규화한 수치로 선원의 상대적 위험을 평가하는 기초적인 기준으로 사용)에 대한 기준을 추가적으로 적용 하여 국내 폐밀봉선원 분류체계에 대한 방안을 제시한 후, 각 범주에 대한 처분방식을 도출하였다. 다양한 처분시점을 상정 한 국내 폐밀봉선원 특성 분석 및 처분방안별 대상 수량·체적 평가결과를 통해 본 연구에서 도출된 처분방안을 처분 예상 시기와 무관하게 2015년 3월말 기준으로 임시저장 중인 모든 폐밀봉선원에 대해 적용할 수 있음을 확인하였다. 단, 방사능 량을 확인할 수 없거나 비방사능 또는 A/D 값을 산출할 수 없는 선원에 대해서는 본 연구결과를 적용할 수 없으므로 처분방 안 이행을 위해서는 사전에 비방사능, 체적 등의 선원 고유 특성이 반드시 확인되어야 한다.

The study aims to assess the embryo development and survivability of bovine embryos cultured in vitro by addition of cysteine. The rates of metaphase II formation are not significantly different among the three groups(73.8% for TCM199, 76.9% for TCM199 with 0.3mM cysteine and 83.8% for TCM199 with 0.5mM cysteine). No differences on cleavage rate(70.6~74.6%) was observed among three culture medium(70.6% for TCM199, 71.3% for CR1aa, and 74.6% for SOF) with 0.5mM cysteine. However, significantly(P<0.05) higher development rate was obtained in the blastocyst stage by adding 0.5mM cysteine in SOF medium(35.6%) than in TCM199(27.6%) or CR1aa(26.6%). No significant differences in the cleavage rates were observed among the three culture. After freezing the blastocysts cultured with 0.5M cysteine, the re-expansion rates ranged from 61.3% to 86.4% among groups, and hatching rates are from 26.3% to 46.9% among groups. The rates of re-expansion and hatching are significantly(P<0.05) higher in SOF medium(86.4% and 46.9%, respectively) than those in TCM199(61.3% and 26.3%) and CR1aa medium(87.1 and 44.4%). After thawing, the blastocyst re-expansion rate become significantly(P<0.05) higher in in vivo (87.1%) and in vitro (70.3%) embryos. In conclusion, our results demonstrate that supplementation of IVM and IVC media with 0.5mM cysteine improved the quality of in vitro production of embryo and post-thawed embryo. Future studies comparing these culture systems in well-designed trials should be performed.

본 실험은 종자 가격이 비싼 여주를 삽목하여 증식하고자 할 때 적절한 줄기 절단위치와 삽목상내 온도를 구명하기 위하여 실험하였다. 시험품종 ‘NS454’(NS)와 ‘드레곤’(DR) 품종을 대상으로 삽수 절단 위치를 3번째 잎의 마디를 절단한 처리(I) 와 3번째와 4번째 잎의 중간없이 발근이 되지 않았다. 뿌리의 개체당 발근수는 DR 품종의 경우, 28oC 처리구는 마디 중간지점을 절단한 처리구에서 5.3개로 처리구중에서 가장 많았고 마디를 절단한 처리구는 2.7개이었다. 따라서 여주의 삽목시 절단위치는 마디부위를 절단하거나 마디 중간지점을 절단해도 되지만 마디 중간지점을 절단하는 것이 생존율을 높일 수 있을 것으로 사료되고, 또한 삽목상내 기온을 28oC 정도를 유지해 주는 것이 바람직할 것으로 판단된다.

This paper adopts a multi-factorial analysis and investigates English dative alternations in Korean EFL learners’ writings, by directly comparing dative alternations of native speakers and those of Korean EFL learners’ writings. Two corpora were chosen for the comparison: the Switchboard corpus (written sections) and the Korean component of the TOEFL11 corpus. After all the sentences with dative alternations were extracted from these two corpora, and seventeen linguistic factors were manually encoded, the data were statistically analyzed in R. This paper was theoretically based on the concept of interlanguage in the Second Language Acquisition and Competition Model of Bates and MacWhinney (1982, 1989). Through the analysis, the following facts were observed: (i) the Korean EFL learners used ditransitive constructions more frequently than prepositional constructions, (ii) eight main factors and four interactions with the L1 were statistically significant, and (iii) the ditransitive constructions which the Korean EFL learners used were closer to the prepositional constructions rather than the ditransitive constructions in Korean.

이 연구는 집중인성교육 프로그램의 Ⅰ·Ⅱ·Ⅲ 과정에 대한 프로그램의 운영, 교육내용, 교육강사 운용 등을 중심으로 문제점을 검토하고 개선방안을 제시하는 것을 목표로하였다. 이를 위하여 2015년 4월 현재 집중인성교육을 시행하고 있는 4개 교도소의 프로그램내용과 강사구성 등을 분석하였다. 연구를 진행하는 과정에서 수형자의 인성교육을 통해 재범억제는 물론 건전한 사회복귀를 추구하는 집중인성교육 프로그램에서 몇 가지 문제점을 확인하고, 개선방안을 제시하였다. 먼저, 프로그램의 운영과 관련하여, ① 프로그램의 연속성 확보를 위한 제도개선, ② 수용자의 자발적 참여를 유도하는 인센티브 개발, ③ 교육프로그램의 목적 명확화, ④ 프로그램에 대한 평가와 피드백을 통한 한국형 인성교육 프로그램 모델 개발 등이 그것이다. 다음으로, 집중인성교육 프로그램 내용과 관련하여, ① 단순 강의식 교육을 탈피하여 흥미와 참여를 유발할 수 있는 각종 교육기법의 활용, ② 시설 밖 프로그램의 활용 및 교류방안 모색, ③ 주도적 학습 프로그램의 일부 운영, ④ 죄종별 차별화된 인성교육 프로그램의 개발 등이 그것이다. 끝으로, 집중인성교육 교육강사 운용과 관련하여, ① 강사 관련 예산의 확보, ② 지역사회 전문가 집단 인력풀의 확보, ③ 내부강사의 재교육 및 위탁교육 내지 전종직원의 채용 등을 제안하였다.

An FDA approved drug for the treatment of type II diabetes, Troglitazone (TRO), a peroxisome proliferator–activated receptor gamma agonist, is withdrawn due to severe idiosyncratic hepatotoxicity. In the search for new applications of TRO, we investigated the cellular effects of TRO on YD15 tongue carcinoma cells. TRO suppressed the growth of YD15 cells in the MTT assay. The inhibition of cell growth was accompanied by the induction of cell cycle arrest at G0/G1 and apoptosis, which are confirmed by flow cytometry and western blotting. TRO also suppressed the expression of cell cycle proteins such as cyclin D1, cdk2, cdk4, cyclin B1, cdk1(or cdc2), cyclin E1 and cyclin A. The inhibition of cell cycle proteins was coincident with the up-regulation of p21CIP1/WAF1 and p27KIP1. In addition, TRO induces the activation of caspase-3 and caspase-7, as well as the cleavage of PARP. Further, TRO suppressed the expressions of Bcl-2 without affecting the expressions of Bad and Bax. Overall, our data supports that TRO induces cell cycle arrest and apoptosis on YD15 cells.

사이닝 보너스 지급에 관한 약정을 함에 있어서, 대상자에게 일정한 기간을 의무적으로 근무할 것을 규정하였으나, 위 의무 근무기간을 위반 하는 경우에 동 금액의 반환에 관하여는 명문의 약정을 하지 않는 경우 가 있다. 이러한 경우에, 의무 근무기간 위반 시에 대상자가 사이닝 보너스를 반환해야 하는지 여부는 사이닝 보너스의 법적 성격을 어떻게 이해할 것 인지에 따라서 달라지게 된다. 이에 대하여 대상 판결은, 사이닝 보너스가 이직에 따른 보상이나 근 로계약 등의 체결에 대한 대가로서의 성격에 그칠 뿐이라면 계약 당사자 사이에 근로계약 등이 실제로 체결된 이상 근로자 등이 약정근무기간을 준수하지 아니하였더라도 사이닝 보너스가 예정하는 대가적 관계에 있는 반대급부는 이행된 것으로 볼 수 있다고 판시하였는 바, 이는 타당하다. 또한 대상 판결은, 사이닝 보너스가 이직에 따른 보상이나 근로계약 등의 체결에 대한 대가로서의 성격만 가지는지, 더 나아가 의무근무기간 동안의 이직금지 내지 전속근무 약속에 대한 대가 및 임금 선급으로서의 성격도 함께 가지는지는, 해당 계약이 체결된 동기 및 경위, 당사자가 계 약에 의하여 달성하려고 하는 목적과 진정한 의사, 계약서에 특정 기간 동안의 전속근무를 조건으로 사이닝 보너스를 지급한다거나 기간의 중간 에 퇴직하거나 이직할 경우 이를 반환한다는 등의 문언이 기재되어 있는 지 및 거래의 관행 등을 종합적으로 고려하여 판단해야 한다고 판시하였 는 바, 이 역시 타당하다. 그러나 대상 판결은 위 구별기준들을 본건 소송에 구체적으로 적용함 에 있어서, 일부의 기준만을 지나치게 편중되게 강조한 나머지 다른 기 준들을 무시하거나, 또는 위 구별기준들에 포함되어 있지 않은 점들을 적용 단계에서 새로운 기준으로 추가하는 등으로 논리적 일관성을 갖추 지 못한 것으로 평가된다.

Calcipex II, calcium hydroxide paste, is used widely as intracanal dressing material during root canal treatment. When extrusion of calcium hydroxide through root canal occurred, various tissue reactions have been reported. Herein, we report a case of chronic maxillary sinusitis induced by extrusion of Calcipex II. A 20-year-old male was referred our institution during #26 root canal treatment. Radiographically, it was presented radiopaque round mass with sinus mucosal thickening. The mass was excised and analyzed histologically. Histology revealed Calcipex II granules engulfed by macrophages and chronic maxillary sinusitis around Calcipex II granules.

본 연구는 USCG phase-II의 형식승인 기준인 자연상태 생물군집의 75 % 이상 유지하여야 하는 평가체계에 대비하여 자연생물군집 농축 및 선박평형수관리시스템(Ballast Water Management System, BWMS) 처리 전 후 생물사멸시험을 실시하였다. 자연 식물플랑크톤군집의 농축 조사는 중영양수계인 장목만과 부영양화수계의 마산만에서 동계에 수행하였다. 장목만과 마산만에서 1톤 기준으로 생물을 농축하였을 경우, 10-50 μm 크기 생물 현존량은 4.7 × 10⁴ cells mL-1과 0.8 × 10⁴ cells mL-1 이었고, 농축생물의 생존율은 90.4 %와 88.0 %로 각각 나타났다. 특히 장목만에서는 Skeletonema costatum-like species 같은 체인을 형성하는 소형 규조류가 극우점한 반면, 마산만에서는 <10 μm보다 작은 편모조류 및 체인을 형성하지 않는 대형 와편모조류(Akashiwo sanguinea, Heterocapsa triquetra)가 우점하였다. 이와 같은 우점종 세포크기의 차이로 장목만 농축효율이 마산만보다 높게 나타났다. BWMS 장비를 통과한 처리 당일 생물 사멸률은 장목만이 90.4 %로, 마산만의 93 %보다 약간 낮았고, 장목만에서 BWMS 처리 5일 경과 후, 대조군의 대상생물의 사멸률은 6.7 %로 나타났다. 처리군에서는 >99 %로 대부분 사멸되어, 시험생물로서의 적용 가능성을 확인할 수 있었다. 결과적으로 동계와 같이 해역내 생물량이 낮을 경우, 주간 8시간 수행한 네트의 생물농축만으로는 USCG Phase II의 형식승인 기준인 500톤 탱크에 1.0 × 10³ cells mL-1 이상으로 자연생물 개체수 밀도를 충족하기는 쉽지 않다는 것을 파악하였고, 이를 보완하기 위해서는 일정기간 자연생물을 대량 배양 및 채집할 수 있는 시스템 도입이 필요할 것으로 판단된다.

Advertising both reflects and creates social norms and cultural practices, such as concepts of beauty and gender roles. Research suggests that masculinity, like femininity, is constructed, codified and contested in advertising imagery (Schroeder & Zwick, 2004). By drawing on cultural categories to depict gendered consumer selves, advertising messages often limit and structure possibilities of masculine and feminine consumption. As marketers promote the blurring of traditional gender lines around product categories to open their products to a wider market, men increasingly consume products that are traditionally reserved for female consumption (Thompson & Hirschman, 1995). Despite the growing global men’s grooming market, research suggests that men view the consumption of cosmetics as not acceptable ‘masculine’ consumption behavior (Hall, Gough, & Seymour-Smith, 2013). According to Kolbe and Albanese (1996), masculinity is represented in advertising by images of strong and muscular ‘male icons’. In order to protect their masculine identities men reject advertising images that do not reflect these masculine traits (Elliot & Elliot, 2005). However, with advertising literature focusing on a notion of masculinity that is prevalent in Western individualistic cultures, cross-cultural research in this area is extremely limited. Given the cultural relativity of masculinity and attractiveness, images of masculinity and forms of accepted ‘masculine’ consumption behavior are likely to vary across cultures (e.g., Englis, Solomon, & Ashmore, 1994). For instance, the use of cosmetics may be regarded as acceptable ‘masculine’ behavior in South Korea, where young men spend more per-capita on cosmetics than their counterparts anywhere else in the world (Euromonitor, 2015). The aim of this research is to explore representations of masculinity in South Korean cosmetics advertising. We carry out a content analysis of print ads examining i) What types of male images do advertisers use in South Korean cosmetics advertisements?; and ii) What kind of masculinity do male images in South Korean cosmetics ads represent?

This study (i) examines the main effect of how a customer’s trust in the service personnel could affect his/her service co-designing and co-delivering behavior; and (ii) investigates how the main effect could vary by the customer’s trust in the service brand, and the types of customer contact service contexts. Keywords: customer participation, co-

Organisations encourage shareholders to invest in the place and the place audience relies on place reputation when making investment decisions and product choices. Given the significance of the place branding and place heritage and building upon the evidence discussed, this research is one of the first attempts at collecting empirical evidence that seeks to prove that a favourable place branding and place branding heritage influence a favourable place image and favourable place reputation. This study aims to explore employees and visitors/consumers’ perceptions and practices regarding the place branding and the main factors that influence place branding suitability at a visitor/consumer/employee level. By achieving these objectives, it is expected that the investigation will add to current knowledge about the place branding and provide practical insights to managers and decision-makers. Based on the research objectives of this study, three overall research questions are: (i) What are the factors that influence place branding favourability, (ii) What are the main influences of place heritage favourability on favourable place branding?, and (iii) What are the main influences of place branding favourability on favourable place image and favourable place reputation? This research addresses the general goals: first, it explores the concept of the place branding and its dimensions. Second, it identifies the factors that are most likely to have a significance influence on the favourable place branding (antecedents of the favourable place branding). Third, it develops and empirically assesses a model concerning the relationships between favourable place branding, its antecedents and its consequences. Fourth, it examines the influence of the favourable place heritage on place branding. Finally, it investigates the impact of the favourable place branding on favourable place image and favourable place reputation (consequences of the favourable place branding). Despite the potentially significant role of the favourable place branding, little empirical research has examined how the favourable place branding exposes corporations and their members to far greater scrutiny. Creating a employee/consumer/visitor level model based on attribution theory demonstrates the issues retailers face in relation to place branding: (i) the association between the place branding concept and its elements that foster or discourage; (ii) its benefits or outcome for place; (iii) the relationships between other theoretically and empirically identified variables. In order to fill this gap in the academic literature, prior studies and the insights gained from current field research were reviewed. The model and various propositions developed thereafter, merit further study.

Major changes are challenging the tourist industry, such as new entrants, suppliers’ direct sales without intermediaries, and customers’ bargaining power due to Internet services, among others. In this context, the aim of this research is to assess the influence of two emerging constructs, eWOM adoption and customer engagement, jointly with consumer trust and brand equity, on travel agency loyalty. There is a huge amount of research available regarding the variables considered in this study: (i) brand trust and equity, and brand loyalty, have always been considered in the marketing literature; (ii) engagement and eWOM adoption have aroused interest from researchers since online comments gain popularity and usefulness. But their consideration in literature has been based, in most of the analyses, on symmetric relationships and it then fails to recognize the occurrence of causal asymmetry. In the present research a novel methodology is adopted, fuzzy set Qualitative Comparative Analysis (fsQCA), which uses Boolean algebra to show how causal conditions combine to bring about outcomes. On a sample of 520 travelers and through a fuzzy-set Qualitative Comparative Analysis, data shows that brand trust and brand equity are key drivers of loyalty, measured as a repurchase intention. In fact, jointly both variables lead to travel agency loyalty and when no engagement-enthusiasm dimension exists, for individual repurchase intention, brand equity or brand trust are also needed. Moreover, just engagement in terms of interactions also leads to brand loyalty, but engagement-enthusiasm dimension needs support of eWOM adoption to impact travel agency repurchase intentions. This finding highlights the specific importance of each analyzed variable as key drivers of travel agency loyalty. Theoretical and managerial implications are provided based on results.