This study examined the relationships among indicators, distributional differences between connected and disconnected reaches, and the latent structure of indicators within the current lateral connectivity assessment framework. The lateral connectivity assessment system evaluates the physical connection between streams and floodplains using indicators related to channel cross-section, levee slope, culverts, vegetation cover, urbanized area, and wetland area. However, empirical understanding of whether these indicators operate independently and how they differ between connected and disconnected reaches remains limited. To address this gap, Mann-Whitney U tests, Spearman correlation analysis, and exploratory factor analysis based on a Spearman correlation matrix were conducted. The results showed that connected and disconnected reaches differed significantly in most indicators, including channel cross-section, vegetation cover, culverts, riverside slope, landward slope, and urbanized area. Spearman correlation and exploratory factor analysis indicated that major physical structural indicators, such as channel cross-section, levee slope, and culverts, tended to vary together and loaded on the same factor. These findings suggest that the current assessment framework effectively reflects physical connectivity between streams and floodplains, while also indicating that some structural indicators may represent overlapping characteristics. In contrast, wetland area showed weak associations with other indicators, formed an independent factor structure, and did not significantly differ between connected and disconnected reaches. Therefore, wetland-related indicators should be improved by incorporating functional connectivity, such as hydrological connection, flood inundation frequency, and residence time, rather than relying solely on area-based measures. This study provides empirical evidence for interpreting and refining lateral connectivity assessment indicators.
Cristaria plicata, a freshwater bivalve designated as an endangered species, inhabits buried beneath the substrate and releases somatic cells into the water during filter feeding. Therefore, environmental DNA (eDNA) can enhance the efficiency of detecting C. plicata. In this study, we assessed the possibility for detecting freshwater bivalves including C. plicata through metabarcoding of eDNA. In March 2024, water samples were collected at Cheongju, Gunsan, and in the Nakdong river, where sampling sites were selected based on the inhabitation of C. plicata. After eDNA extraction using GF/F filters and DNeasy Blood&Tissue Kit, mitochondrial 16S rDNA of freshwater bivalves was amplified using Unio 16S primer and analyzed via metabarcoding. C. plicata eDNA was detected at most sites according to the confirmed presence of C. plicata. However, at some sites in the mainstream of Nakdong river where C. plicata was known to inhabit, C. plicata eDNA was not detected. Changes in the riverbed environment caused by artificial factors may have negatively affected the habitat of C. plicata. And the high proportion of unassignment and the accumulation of eDNA at the littoral zone may be associated with the continuous inflow, as well as changes of depth and substrate in the lotic ecosystem. This study demonstrates that using eDNA for C. plicata detection enables to enhance the efficiency of surveys.
Amphibians are the most threatened vertebrate group worldwide, largely because their biphasic life cycle depends on both aquatic and terrestrial environments and is highly sensitive to fine-scale hydrological variation. Under accelerating habitat loss and climate-driven shifts in rainfall regimes, substitute habitats are increasingly used as a practical conservation measure. Yet their effectiveness cannot be assumed from physical construction alone. When such sites attract individuals but fail to sustain breeding and recruitment, they may function as ecological traps rather than compensatory habitats. This concern is especially acute for the Boreal digging frog, Kaloula borealis, an explosive breeder whose reproductive success depends on the persistence of temporary aquatic habitat during the larval period. We integrated 191 field surveys from four substitute habitats with daily NASA POWER meteorological data and analyzed habitat performance using a weather-normalized framework that accounted for climatic variability among survey periods. The analyses revealed a clear decoupling between structural performance, defined as the capacity to retain breeding-season water, and functional performance, defined as the capacity to translate retained water into aquatic breeding opportunity. Incheon- Geomdan showed the strongest structural buffering, whereas Gwangmyeong-Siheung exhibited the highest functional breeding performance. Cheonan-Jiksan maintained intermediate but balanced performance across both dimensions, while Asan-Tangjeong was consistently poor in both, indicating potential sink-like characteristics. These findings show that restoration success in amphibian substitute habitats cannot be reduced to a single metric or to breeding records from a single year. Habitat evaluation should instead distinguish settlement, hydrological persistence, and breeding function, and interpret them under standardized climatic conditions. This framework provides a stronger basis for adaptive management and for designing substitute habitats that support long-term population viability rather than temporary occupancy.
Frog call classification in field recordings is difficult because vocalizations vary with background noise, recording distance, and calling context. This study presents an embedding-based transfer-learning framework for practical species identification using Perch2 as a pretrained bioacoustic feature extractor. Recordings from seven frog species (69 files) were scanned with overlapping 5-s windows, band-pass filtered between 150 and 6000 Hz, and ranked by RMS energy; up to eight high-energy windows per file were retained, yielding 441 clips. Each clip was converted into a 1536-dimensional Perch2 embedding, standardized, and classified with L2-regularized multinomial logistic regression using five-fold grouped cross-validation in which clips from the same recording were assigned to the same fold. The model achieved an overall out-of-fold accuracy of 0.868 at the clip level and 0.884 at the file level. Fold-level accuracy was 0.873±0.094 for clips and 0.885±0.081 for files. The logistic-regression model consistently outperformed an XGBoost baseline trained on the same embeddings, although paired fold-level tests were interpreted cautiously because only five folds were available. Embedding-space diagnostics showed statistically non-random species structure, but low silhouette values indicated partial rather than complete species separation. Within-file embedding variability showed a weak but statistically supported negative association with file-level confidence (Spearman ρ= - 0.299, p=0.013). These results support Perch2 embeddings with a simple regularized linear classifier as a practical, interpretable approach for amphibian acoustic classification in data-limited settings, while indicating that confidence scores should be used as review-prioritization aids rather than calibrated occurrence probabilities.
Prioritizing candidate OECMs in Korea requires an ex ante framework that compares institutional constraints across candidate types while mitigating data deficiency and selection bias before site-level assessments are finalized. This study groups nationally discussed candidates into 17 candidate types and represents OECM suitability through five latent axes bounded between 0 and 1: non-protected area status (nonPA), boundary clarity (boundary), governance stability (governance), long-term durability (longterm), and biodiversity outcomes (biodiv). Axis scores are modeled with Beta priors whose means and uncertainties are elicited via transparent, rule-based flags extracted from structured textual descriptions, thereby preserving traceability of how assumptions shift each axis. Joint dependence among axes is introduced using a copula, and an additional directional mechanism links governance to long-term durability to reflect the institutional premise that durable outcomes are underpinned by stable authority, responsibility, and coordination capacity. Decisions are evaluated with a two-stage rule consisting of a minimum-threshold gate across all axes and a weighted composite score applied to gate-passing samples. Complementary analyses examine single-axis mean-shift interventions, rank stability under alternative weight interpretations, and expected utility combining registration likelihood with effective area. Results indicate that feasibility is shaped less by average levels than by bottlenecks generated by simultaneous minimum requirements. Recurrent bottlenecks concentrate in boundary documentation and spatial attribution, outcome-evidence systems, and the justification for reporting units distinct from protected areas. The analysis further reveals a structural separation between types that are relatively easy to register and types that yield larger expected gains once registered, supporting a staged policy portfolio that couples near-term standard-setting through readily registrable types with medium-to-long-term investment in high-impact types. Across most types, governance strengthening emerges as the most consistent leverage for increasing final pass probabilities, suggesting that improvements in durability and overall feasibility are more efficiently achieved through institutional alignment than through isolated, single-axis adjustments.
This study was conducted to analyze the growth of oak species, which are the most widely planted in ecological restoration sites established as part of the Four Major Rivers Riverin Ecological Belt Project, and to propose an appropriate management period based on these findings. Generally, transplanted trees undergo artificial operations such as root pruning or pruning, and such physical disturbances are a significant cause of reduced growth immediately after planting. The time required to recover pre-transplant growth varies depending on the tree species; therefore, it was determined that analyzing the changes in annual growth of oak species and establishing an appropriate management period based on the results is crucial for stable establishment. Accordingly, this study analyzed the growth of oak species planted in the riverin ecological belt sites of the Geum River and Nakdong River basins and compared annual growth rates before and after planting. Furthermore, the study aimed to identify the time required for full-scale volume growth after planting and propose an appropriate management period based on these results. The research results showed that, similar to other tree species planted in ecological restoration sites, the growth rate of oak trees decreased sharply immediately after transplantation, but began to recover starting from the third year after planting. It was confirmed that it takes between three and five years to recover the growth rate to pre-transplantation levels. As oak trees are a major species planted in riverin ecological belt creation projects, their survival rate is a critical factor determining the success or failure of the project. By providing objective data on the growth patterns of oak trees after transplantation, this study can serve as important foundational data for establishing management plans to reduce tree mortality rates.
Freshwater unionid bivalves are difficult to monitor due to their cryptic, burrowing behavior and their residence in soft substrates. DNA barcode reference sequences are essential for molecular identification and conservation studies of endangered freshwater unionids. For endangered species, the collection of live individuals is often restricted, which limits the construction of such reference databases. In this study, we evaluated the feasibility of using shell-derived DNA from Cristaria plicata as a source for mitochondrial DNA barcoding. Naturally deposited shells were collected from a freshwater environment, and DNA was extracted from five distinct shell regions under different preservation conditions. Mitochondrial 16S rDNA was targeted using both short (180 bp) and long (450 bp) barcode primers. PCR products were sequenced and compared with GenBank reference sequences, and phylogenetic analyses were conducted to confirm taxonomic identity. DNA yield differed substantially among shell regions and preservation states. Phylogenetic analyses demonstrated that sequences derived from shells clustered with established C. plicata references from South Korea and other East Asian populations. However, degraded shell materials occasionally produced divergent or nonspecific sequences, particularly when longer barcode regions were amplified. These results indicate that shell-derived DNA can serve as a viable, noninvasive source for generating DNA barcode references of endangered unionid bivalves. This approach may facilitate the expansion of reference databases and support molecular monitoring and conservation efforts without requiring the collection of live specimens.