A Transgenic Kimch cabbage has been developed harboring T-DNAs expressing delta-endotoxin insecticidal protein, herbicide (basta) resistant protein, and antisense transcript of AsMADS2 gene. Three transgenic lines, #24, #45, and #51, originated from the same T0 plant were analyzed in terms of molecular characterization, phenotype, and agronomic traits. Flanking sequence analysis confirmed that T-DNA, with 7132 bp intact structure, was inserted onto the pseudochromosome A10 of B. rapa and all the genes in T-DNA were functionally active. Three of GM cabbage showed 69.2～75.3% of plant height and 81.8～89.7% of diameter to those of the isogenic variety ‘Nowon’, respectively. Curving upward leaf lamina attitude was observed on GM cabbage, while straight or slight concave on non-GM cabbage. In addition, an average range of 86～91.5% of head height and 87.4～94.8% of head diameter were observed on GM cabbage to those of the isogenic variety ‘Nowon’, respectively Moreover, curled inwards or slight overlap of head-forming leaf overlap at terminal region was observed on GM cabbage, but curled outwards or erect on non-GM cabbage. AsMADS2, a transcription factor reported to be involved in early flowering, was stably expressed to RNA in the GM cabbage, but it was not shown the significant influences to flowering time.

Bt gene derived from the B. thuringiensis has been used for developing GM crops and those crops are already on the market. The aim of this study is to construct a genetic transformation with peppers using CryIAc1 gene and consequently to develop GM peppers resistant to the oriental tobacco budworm. We have developed transgenic peppers using Agrobacterium-mediated transformation, and obtained 5 T0 peppers. T0 peppers were self-crossed and T1 pepper fruits were exposed to larvae to test the survival rate. The survival rate of larvae that were fed with GM fruits decreased dramatically while with non-GM fruits the rate was not changed much. In order to establish the selection and the culturing of bug through a year, an incubating system for tobacco budworm in chamber was manufactured and a selection system under the media that are mixed with GM green pepper was obtained. Using those system, T3 peppers tolerant to tobacco budworm were selected.

Environmentally inflicted stress (abiotic stress) such as high drought stress could be limiting the plant productivity. The mechanism of drought stress signaling in plant related with anti-apoptosis has not yet been full described. Understanding drought stress signaling is key to producing drought-tolerant plant. In this study we recently have identified Oryza sativa genes related abiotic stress water deficit. Abiotic stress related genes were screened from Oryza sativa cDNA library and identified gene by yeast functional screening. The yeast expression showed that they east cell grow well on SD-galactose-Leu-Ura-. The screening of over than 7000 clones from Oryza sativa cDNA libraries has been identified. 28 clones that survived following BAX-expression on inducible galactose medium. R12H780 clones confirmed protein prediction like putative senescence-associated-protein. This gene contains an open reading frame (ORF) of 108 amino acids. Transcription of R12H780 was induced in response to drought stresses, RT-PCR analysis showed transcript level in plant strongly detected in earliest time of drought stress treatment. Yeast transformed with R12H780 gene displayed markedly improved tolerance to PEG treatment, and high salinity in comparison to the control yeast (vector only). The results indicate R12H780 expression represents a new type of drought stress related gene with anti-apoptotic in Oryza sativa and endows tolerance to several types abiotic stress.

In this study, we generated and characterized the transgenic rice plant expressing a spider silk protein. A cDNA coding for the C-terminus of spider dragline silk protein (AvDrag) was cloned from the spider Araneus ventricosus. Analysis of the cDNA sequence shows that the C-terminus of AvDrag consists of 165 amino acids of are petitive region and 99 amino acids of a C-terminalnon-repetitive region. The peptide motifs found in spider drag line silk proteins, GGX and An, were conserved in the repetitive region of AvDrag. The AvDrag cDNA was expressed as a 28kDa polypeptide in baculovirus-infected insect cells. To produce transgenic rice plant with high contents of glycine and alanine, the prolamin promoter-driven AvDrag was introduced into rice plant via Agrobacteriumtumefaciens-mediated gene transformation. Because of seeds pecific prolamin promoter, expression of AvDrag protein has been achieved inriceseed. The introduction and copy number of the AvDrag gene in transgenic rice plants were determined by PCR and Southern blot analysis. AvDrag expression in transgenic rice seeds was examined by Northern blot and Western blot analysis. Immuno fluorescence staining with the AvDrag antiserum revealed that the recombinant AvDrag proteins were localized in transgenic rice seeds. Furthermore, the amino acid content analysis showed that transgenic rice seeds were greatly increased in glycine and alanine as compared to controls. The present study is the first to show the expression of spider silk protein in rice seed.

Daily shedding pattern and longevity of pollen are important consideration for the evaluation of gene flow of transgenic plants. During the day, the pollen shedding pattern of zoysiagrass was determined in the lawn by using a device to collect airborne pollen on a glass slide, resulting that the pollen grains were released predominantly between 7:00 and 9:00. The result was also supported by in vitro pollen germination test, which was performed with pollens collected from 1:00 through 24:00 at 1h interval. Influence of temperature and humidity on pollen longevity was determined by germinating pollen at 25°C after incubating them for 10, 30, 60, and 180 min under different temperatures and humidity with pollen of zoysiagrass that opened freshly at about 9:00. The result showed that pollen longevity of zoysiagrass was sensitive to change of temperature and humidity and longest under the temperture and humidity of 15-20°C and 80-99%, respectively. Under natural conditions with the same method as upper controlled conditions, was determined pollen longevity. Under sunny atmospheric conditions, pollen longevity decreased to 20% in 60 min, with a complete extinction in 120 min. Under cloudy atmospheric conditions, pollen remained viable up to 450 min, with about 20% longevity after 360 min. No significant difference was found between GM and non-GM plants in their pollen longevity.

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced a MsHsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confirmed by PCR, northern blot, and western blot analyses. Under normal growth conditions, there was no significant difference in the growth of the transgenic plants and the non-transgenic controls. However, when exposed to various stresses such as salt or arsenic, transgenic plants showed a significantly lower accumulation of hydrogen peroxide and thiobarbituric acid reactive substances than control plants. We speculate that the high levels of MsHsp23 proteins in the transgenic plants protect leaves from oxidative damage through chaperon and antioxidant activities. These results suggest that MsHsp23 confers abiotic stress tolerance in transgenic tall fescue and may be useful in developing stress tolerance in other crops. Compared with traditional plant breeding, genetic engineering provides a relatively fast and precise means of achieving improved stress tolerance of forage crops. Development of forage crops that are more tolerant to various abiotic stresses could lead to the use of more new lands for cultivation.

CGMMV (cucumber green mottle mosaic virus) infects many cucurbitaceae species causing mosaic symptoms, yellowish leaf, and fruit deterioration. Several isolates of CGMMV from Korea, Israel, Japan, Greece and Spain have been characterized. In fact, CGMMV outbreaks have caused huge losses in the total yields of cucurbitaceous crops in Korea. Unfortunately, no genetic source is available for the resistance against CGMMV infection, and therefore, the breeding management offers no access to a solution yet. Thus, an alternative was to transform the viral gene such as CGMMV-CP into crops to induce tolerance against CGMMV. Here, we present the successful transformation of watermelon rootstock (gongdae) by Agrobacterium-mediated transformation with the CGMMV-CP gene. We obtained 29 independent T0 watermelon rootstock plants from the transformation of 7,887 explants. T0 plants were self-crossed and T1 plants were screened by the virus infection. Those gongdaes tolerant to virus were selected continuously, and BC2T1 and T3 generation were obtained. We have also obtained watermelons of BCF generation by crossing GM gongdae to watermelon line and those BCF watermelones were strongly tolerant to CGMMV.

Phosphorus (P) is an important structural component and plays critical roles in the process of energy transfer and signal transduction. Effect of low P on carbohydrate metabolism was investigated at the transcription level via transcriptome analysis using the rice 60K oligonucleotide DNA microarrays. Two-week-old rice seedlings were grown under a low (32 μM) or high (320 μM) P condition for two weeks and leaves from the seedlings were used for transcriptome analysis. Expression of genes involved in carbohydrate metabolic pathways (eg. glycolysis, sucrose degradation and starch synthesis and degradation) was most significantly affected under low P. Under low P, most genes involved in glycolysis were intensively down-regulated, genes of starch biosynthesis and degradation pathway were up- or down-regulated, and many genes involved in sucrose biosynthesis were intensively up-regulated. In leaves under low P, glucose and pyruvate levels decreased, but sucrose and starch levels increased. These results suggest that carbohydrate metabolism is adjusted primarily through comprehensive transcriptional modulation of genes involved in the carbohydrate metabolic super-pathway.

In pepper, investigation of important traits such as disease resistances, high yield and pungency were mostly focused on a cultivated species, Capsicum annuum. This narrow breeding pool hampered to develop improved cultivars. Exploitation of wild germplasm in Capsicum has been recognized as an important issue. The construction of core collection and analysis of genetic diversity in Capsicum is the first step to make full use of germplasm. Although there have been several attempts to construct core collections in Capsicum, most of the works were limited due to handling small number of samples, relying mainly on the characterization of morphological traits and focusing on C. annuum species. Therefore, the comprehensive studies for genetic diversity and structure of Capsicum including phenotypic data, molecular marker patterns and evaluation of useful alleles are very necessary to understand the structure and patterns of genetic diversity in Capsicum. We are developing for a core collection set in Capsicum using molecular markers and phenotypic data with over 3,000 germplasm accessions.

Targeted gene silencing is an essential component of plant biotechnology. RNA interference is often employed for targeted gene silencing in plants. However, it suffers from off-target effects and unstable gene suppression in many cases. In recent years, engineered nuclease-based tools, such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have been developed to induce site-specific genome modifications. However, these approaches require much time and labor for extensive screening of mutants. We have recently reported that the activities of dimeric transcription factors are competently suppressed by genome-encoded small interfering peptides (siPEPs) that competitively form nonfunctional heterodimers in plants. In addition, some splice variants of transcription factors also act in a similar manner to negatively regulate the activities of specific transcription factors. We designated the siPEP-mediated suppression of transcription factors peptide interference (PEPi). Based on our previous observations, we also developed an artificial siPEP (a-siPEP) approach and evaluated its application for the targeted inactivation of transcription factors in the dicot model, Arabidopsis, and monocot model, Brachypodium. We designed a series of potential a-siPEPs of two representative transcription factors SUPPRESSOR OF OVEREXPRESSOR OF CONSTANS 1 (SOC1) and AGAMOUS (AG) that function in flowering induction and floral organogenesis, respectively. Transgenic plants overproducing a-siPEPs displayed phenotypes comparable to those of gene-deficient mutants. Collectively, our data demonstrate that the siPEP tool is an efficient protein knockout system for inactivating specific transcription factors, and other multimeric enzymes and membrane transporters as well, in plants. We will discuss about the global application of the siPEP toll to other plant species and potential advantages over other gene manipulation tools.

The purposes of this research project are to identify quantitative trait loci (QTLs) associated with yield-related traits using a recombinant inbred line (RIL) population derived from a cross between a high-yield soybean genotype SS0404-T5-76 and Daewonkong and to develop high-yield soybean and lodging-resistant sprout soybean cultivars. For development of DNA markers and identification of functional sequence variations, firstly, whole genome of five soybean genotypes, Sinpaldalkong 2, SS2-2, Pungsanamulkong, SS0404-T5-76 and Daewongkong, were sequenced using Illumina Hi-Seq technology. SS2-2 is a EMS-induced mutant of Sinpadalkong 2. SS0404-T5-76 showing high-yield is a F8 RIL derived from a cross of Pungsanamulkong x SS2-2. Daewonkong is a elite cultivar with high-protein. Furthermore, to construct a genetic linkage map, we are advancing F4 lines of SS0404-T5-76 x Daewonkong by single seed-descent. Secondly, we developed high-protein and high-yield soybean lines and lodging-resistant sprout lines. Area-adaptability tests of these promising lines are performing in three different locations including Jeju, Naju, and Suwon. Based on the results of area adaptability tests, we are planing to conduct cultivar registration of the promising soybean lines.

고추 탄저병은 우리나라는 물론 몬순기후를 갖고 있는 동남아시아의 거의 모든 나라와 중국, 인도 등에서 그 피해가 매우 심각한 병이다. 작년에는 특히 긴 장마로 인해 탄저병 발병이 심하여 엄청난 수확량 감소(홍고추의 경우 대략 50% 이상) 피해를 봤고, 시장에서는 홍고추의 가격이 무려 3배 이상 높게 거래되기도 하였다. 특히 농민들은 비가 올 때마다 농약을 쳐서 농약비용도 아주 큰 부담이 됐다. 따라서 본 과제에서는 지금까지 개발된 탄저병 저항성 계통을 사용하여 하루 빨리 탄저병 저항성 F1 품종을 개발하고 보급해야겠다는 생각으로 여러 종자회사들과 함께 F1 품종 개발에 힘쓰고 있다. 올해 탄저병 저항성 B계통 육성용 33계통(F4세대 30계통, F2세대 3계통), C계통 육성용 106계통(BC2F10세대 36계통, BC2F8세대 47계통, F4세대 23계통), 탄저병/역병 복합저항성 육성용 6계통(F2세대)을 정식하여 육성하고 있다. 특히 작년에는 몇몇 종묘회사의 웅성불임 계통(CMS A계통 또는 GMS 모계)에 본 연구과제에서 개발한 탄저병 저항성 계통을 교배하였고, 올해 총 122개의 F1 조합을 파종한 후 노지포장에 정식하였고, 이들에 대한 탄저병 저항성 및 원예적 특성을 조사할 예정이다. 또한 탄저병 저항성 계통 및 품종을 보호하기 위해 탄저병 저항성 유전자원 Capsicum baccatum ‘PBC81’과 ‘PI594137’ 및 탄저병 저항성 육성계통 ‘2602-14-5’와 ‘2602-27-21’을 NGS sequencing하였고, 염기서열을 비교 분석하였다. 이 결과를 바탕으로 탄저병 저항성 계통 및 품종 보호를 위한 특허를 출원할 예정이다.

Genome-wide association study (GWAS) is a very powerful method to identify the natural allelic variation present in crop plants causing variation to economically important traits. The recent advances in high throughput genotyping and sequencing technology supplemented greatly to GWAS. Taking this advantage, we selected a total of 382 Chinese cabbage inbred lines for GWAS study. The selected inbred lines are being sequenced using next generation sequencing technology to develop genome wide gene specific single nucleotide polymorphism markers. The morphological and quality traits data were taken from field grown inbred lines. The phenotype and genotype association study will be done with more environmental grown data’s and developed SNP. At the end of this project, gene specific SNP markers will be developed for Chinese cabbage breeding for morphological and quality traits.

작물의 유전적 개량에서 가장 중요한 부분은 이용 가능한 유전자원으로부터 새로운 유전자 조합을 창출하는 것이다. 야생벼나 잡초벼와 같은 유전자원은 각 지역의 환경조건에 오랜 기간 동안 적응하며 집단을 유지하였기 때문에 여러 가지 저항성이나 불량한 환경에 대한 내성 등 유용한 특성을 갖고 있다. 이러한 야생벼나 잡초벼를 이용하여 양적형질을 개선할 수 있는 유용 유전자를 탐색하는 연구는 국내에서 거의 없는 실정이다. 그러므로 고수량성, 내재해성 등 우량한 유전자를 보유하고 있는 야생벼 유전자원을 교배모본으로 이용하여 이들의 우량 유전자를 선별적으로 재배벼에 이전시키는 육종방법을 개발하고 동시에 우량 품종을 개발하는 것이 필요하다. 본 연구에서는 재배벼의 유전적 배경에 야생벼의 염색체단편이 이입된 근동질계통을 이용하여 수량성 및 내재해성에 관여하는 QTL의 고밀도 지도를 작성하고 관여유전자를 밝히는 연구를 수행하고 있다. 야생벼과 재배벼 (화영벼/W1944) 교잡 유래 이입계통을 이용하여 종자중 관여 QTL (qTGW5)과 수당립수 관여 QTL (qSPP5)이 연관됨을 밝혔다. 야생벼의 수당립수 유전자 (qSPP5)는 재배벼에선 발견되지 않은 야생벼 특이적인 유전자로 판단된다. 직파재배에서 중요한 중배축 신장성에 관한 유전자 고밀도지도 작성을 위하여, 선행연구에서 탐색된 QTL (qMel-1, qMel-3)을 잡초벼/일품벼 조합계통에서 분석한 결과, qMel-1과 qMel-3은 각각 염색체 1번의 RM8260과 염색체 3번 RM426에서 탐지되었으며, 중배축 신장성 관여 유전자의 분리를 위해 microarray 분석 및 association mapping을 실시하고 있다. 유묘기 내냉성 지표로서 저온 조건에서의 엽록소 함량에 관한 QTL(spad-1, spad-4)이 밀양23/합천앵미3조합에서 탐지되었으며 , 이들 유전자의 근동질계통 (NIL) 육성, 후보유전자의 T-DNA 삽입변이체를 분석 중에 있다. 내건성 QTL의 고밀도 유전자지도 작성을 위해 밀양23/O. glaberrima 조합을 이용, NIL를 육성 중에 있다. 야생벼인 Oryza grandiglumis와 O. rufipogon에서 유래된 종자중 관여 유전자 qGW2와 qGW9를 집적한 계통을 육성하고 그 특성을 평가 중이다.

The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is one of the most serious insect pests affecting cultivated rice (Oryza sativa L.) in temperate regions of East Asia. To understand the genetic basis of the GRH resistance, a F2 population derived from across between a highly resistant variety,Cheongnam and a susceptible variety, Junambyeo was analyzed by genetic analysis and association mapping. GRH resistance was evaluated using the F2 populations. The results showed that a single dominant gene in Cheongnam. DNA from 22 F2 individuals being either resistant or susceptible were pooled to produce bulk resistant and bulk susceptible DNA samples. Parents and bulks were screened with 192 SSR markers and twolinked SSRmarker, RM6082 and RM20145 were identified.Subsequent mapping in the original mapping population showed that thelocusis flanked by the SSR markers, RM20130 and RM20152 on chromosome 6. To physically map this locus, the-linked markers were landed on the artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. The DNA markers found to be closely linked to Grh3 would be useful for marker-assisted selection for the improvement of resistance to GRH in rice.

Recently there are many reports that signaling pathways of abiotic stress and biotic stress are correlated. These relations are not only antagonistic but also synergistic. In this project we are searching the common components in abiotic and biotic stress signaling through proteome and transcriptome analysis. In this project, we are profiling the transcriptome under ABA and biotic stress treatment and searching the common genes which were regulated in both treatment. Furthermore, we are analyzing the secretome and proteome induced under C.maydis. It would be expected that integrative analysis of transcriptome and proteome will presents us the candidate genes to develop abiotic/biotic stress tolerant transgenic plants.

Rice is one of the most important major food crops which provide the major food for more than half of global population. To improve the grain quality as well as grain yield has been the essential breeding goal in rice. The composition of amylopectin is the determinant of rice eating quality under certain threshold of protein content and the ratio of amylose and amylopectin. In this study, RBE 1 driven by CaMV-35S promoter was constructed and transformed using Agrobacterium tumefaciens. We selected single copy with low amylose content among transgenic lines. The mRNA expression was investigated using RT-PCR, and enzyme activity was determined using activity staining method in mid-milky stage endosperm. Also, the overexpression vectors for RBE 1 and SSS 1 driven by seed specific globulin promoter were constructed, respectively. Moreover, the RNA interference vectors for soluble starch synthase 1 and granule bound starch synthase 1 derived by CaMV35S promoter were constructed, respectively and transformed using Agrobacterium tumefaciens. The transgene has been confirmed by amplification of HPT and target gene. The transgenic plants obtained will be used to investigate the gene function of related starch pathway in plant cells using Gopumbyeo as a wild type rice, based on the gain-of-function and the loss-of-function. The development of designed site-specific endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different species. However, the methods described in plants require a highly efficient transformation and regeneration procedure and, therefore, can be applied to very few species. Here, we describe a highly efficient GT system that is suitable for all transformable plants regardless of transformation efficiency. Efficient in planta GT was achieved in rice by expression of a site specific endonuclease (SSS1::ZFN) that not only cuts within the target but also the chromosomal transgenic donor, leading to an excised targeting vector.

작물의 생산량 증대는 농업이 이루는 목표 중 가장 중요한 것 중 하나이다. 본 연구실은 차세대바이어그린 사업의 목표의 일환으로 벼의 생산성을 증가시키는 유전형질을 찾는 것을 목표로 하고 있다. 이에 다양한 벼 유전자의 기능을 분석한 결과 OsVIL2 유전자를 과발현 시키면 생산량이 대폭 증가하는 것을 발견하였다. 유전자 과발현은 이삭 당 열매 수를 50% 가까이 증가시켰으며 또한 줄기 두께 및 신장 길이를 증가시켰다. 따라서 바이오매스가 증가함으로써 추가로 생긴 열매의 무게를 잘 견디는 것이 관찰되었다. 이러한 바이오매스 증가는 세포 수의 증가 때문이며 세포의 길이는 오히려 감소하였다. OsVIL2 유전자는 chromatin remodeling에 관여하는 polycomb group의 subunit 중 하나인 EMF2b와 결합하여 그 기능을 하는 것으로 규명되었다. 이에 본 연구실은 OsVIL2가 어떻게 세포 수를 증가시키는지 세포학적 분석을 할 것이며, 이의 target 유전자는 어떤 것인지 등을 ChIP sequencing을 통해 분석할 예정이다. 또한 이 유전자를 다양한 벼 품종에 도입하여 생산량 증가 표현형과 유전적 배경 사이의 연관관계를 연구할 것이다.

Loss of leaf green color results from chlorophyll (Chl) degradation in the chloroplasts, but little is known about how Chl catabolism is tightly regulated throughout development. Using the stay-green (sgr) mutant in rice which maintains leaf greenness during senescence, we identified SGR by map-based cloning. SGR is a function-unknown gene encoding senescence-induced chloroplast protein. Transgenic rice overexpressing SGR produced yellow leaves, indicating that SGR regulates Chl degradation at the transcriptional level. Leaf stay-greenness of the sgr mutant is mainly associated with a failure in the destabilization of light-harvesting complexes (LHCs) of thylakoid membranes, which is a prerequisite event for the degradation of Chl and LHCs during leaf senescence. SGR binds to light harvesting complex of photosystem II (LHCII), but its biochemical role is so far unknown. During senescence, Chl should be degraded rapidly and safely because Chl catabolic intermediates producing ROS under light are extremely toxic to the plant cells. For safe and rapid degradation of Chl and its catabolic intermediates, Chl catabolic enzymes (CCEs) must catch the Chl intermediates effectively. In recent years, although molecular functions of SGR and CCEs have been characterized in detail, their biochemical mechanism for Chl detoxification remain elusive. Here we show that all five CCEs also specifically interact with LHCII. In addition, SGR and CCEs interact directly or indirectly with each other at LHCII, and SGR is essential for recruiting CCEs in senescing chloroplasts. These data indicate a predominant role for the SGR-CCE-LHCII protein interaction in the breakdown of LHCII-located Chl, likely to allow metabolic channeling of phototoxic Chl breakdown intermediates upstream of nontoxic pFCC.

본 연구는 고추의 다양한 품질 관련 특성 연구를 위한 분자육종시스템 구축의 기초 연구로서 특히 고추의 매운맛과 다양한 색소 등에 초점을 맞추어 연구를 수행하고 있다. 시험재료는 국립원예특작과학원에서 지난 2005년부터 육성해온 고세대(F7) RIL 집단을 이용하고자 하며, 이 집단의 자방친 계통인 “만다린” 품종과 화분친으로 사용된 “블랙클러스터”의 성숙과로부터 발현되는 전사체 프로파일 작성을 위하여 454 Genome Sequencer(GS)-FLX Titanium System을 이용한 전사체 염기서열 분석을 수행하였다. 자방친으로 사용된 “만다린”의 성숙과색은 오렌지색이며, 매운맛이 없는 피망형 품종이다. 화분친으로 사용된 “블랙클러스터”의 미숙과색은 보라색, 성숙과색은 진한 붉은색으로 매운맛이 청양보다 더 높은 매우 작은 삼각형 모양의 과형 특성을 보인다. 염기서열 분석 결과 “만다린”의 경우 총 279,177read(average length=431bp)를 읽었으며, 이들은 총 204,288 contigs와 22,217 singleton으로 조합되었다. 한편 “블랙클러스터”는 총 316,377reads(average length=450bp)가 분석되었으며, 이들은 총 256,630 contigs와 13,153 singleton으로 조합되었다. 분석된 전사체들에 대한 기존 유전자 데이터베이스를 근거로 예상 유전자 기능성 분석 수행 결과 “만다린”은 18개의 FunCat 카테고리로, “블랙클러스터”는 16개의 카테고리로 분류되었다. 크게 세가지 GO(Gene Ontology)로 구분해 봤을 때, Biological process에 관여하는 전사체들은 각각 21%와 24%였으며, Cellular component 관련으로 23%, 27%, 그리고 Molecular function으로 유추되는 전사체들은 23%, 28%를 차지하는 것으로 확인되었다. 본 연구를 통해 확보된 전사체 프로파일과 염기서열들을 기초로 고추 품질 관련 2차 대사물질들의 생합성 과정에 관여하는 전사체들에 대한 발현 비교 분석과 SNP, SSR 등의 마커 개발을 위한 연구가 진행 중이다.