Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.95), catalyzes the reduction of hydroxycinnamaldehydes to give hydroxycinnamyl alcohols, or "monolignols," the monomeric precursors of lignin. Lignins are important components of cell walls and lignified secondary cell walls play crucial roles in long distance transport of water and nutrients during plant growth and development and in plant defense against biotic and abiotic stresses. Here a cDNA clone containing a CAD gene, named as PgCAD, was isolated from a commercial medicinal plant Panax ginseng. PgCAD is predicted to encode a precursor protein of 177 amino acid residues, and its sequence shares high homology with a number of other plant CADS. The expression of PgCAD in adventitious roots and hairy roots of P. ginseng was analyzed using reverse transcriptase (RT)-PCR under various abiotic stresses such as salt, salicylic acid, wounding and chilling treatment that triggered a significant induction of PgCAD at different time points within 2-48 h post-treatment. This study revealed that PgCAD may help the plants to survive against various abiotic stresses.

Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.

인삼의 생장에서 염류의 집적은 우량 인삼의 생산에 많은 장애요인이 되고 있다. 본 연구에서는 순계 분리된 인삼의 우수 계통으로부터 NaCl 처리에 따른 생장율 조사와 ginsenoside의 생산에 미치는 영향을 조사하였다. 선발된 모상근(KGHR-8)으로부터 ginsenosides의 함량에 미치는 NaCl의 최적 농도를 조사하기 위하여 30일간 배양한 결과 NaCl의 농도가 증가함에 따라 모상근의 생장은 감소하였지만, total ginsenoside의 함량은 0.24M NaCl 처리구에서 높은 증가를 가져왔으며 특히 광을 조사하여 배양한 결과 높게 검출되었다. 0.24 M NaCl 농도로 광상태하에서 함량은 61.7% 증가하는 양상을 나타내었다. (Table 1). 또한 모상근의 생장을 최적 상태로 설정하기 위해 two step culture 방법을 조사한 결과, 0.05M, 0.1M NaCl 처리시 모상근의 생장율은 각각 약 62%, 76% 감소한 반면, ginsenoside의 함량은 29%, 48% 각각 향상되었다. 모상근은 방어기작의 일환으로 NaCl을 elicitor로 인지하고 2차대사산물인 사포닌의 생산에 영향을 미치는 것으로 확인되었다.

This study developed a novel augmented reality interface for minimally invasive surgery. The augmented reality technique can alleviate the sensory feedback problem inherent to laparoscopic surgery. An augmented reality system merges real laparoscope image and reconstructed 3D patient model based on diagnostic medical image such as CT, MRI data. By using reconstructed 3D patient model, AR interface could express structure of patient body that is invisible outside visual field of laparoscope. Therefore, an augmented reality system improved sight information of limited laparoscope. In our augmented reality system, the laparoscopic view is located at the center of a wide-angle concave screen and reconstructed 3D patient model is displayed outside the laparoscope. By using a joystick, the laparoscopic view and the reconstructed 3D patient model view are changed concurrently. With our augmented reality system, the surgeon can see the peritoneal cavity from a wide angle of view, without having to move the laparoscope. Since the concave screen serves immersive environments, the surgeon can feel as if she is in the patient body. For these reasons, a surgeon can recognize easily depth information about inner parts of patient and position information of surgical instruments without laparoscope motion. It is possible for surgeon to manipulate surgical instruments more exact and fast. Therefore immersive augmented reality interface for minimally invasive surgery will reduce bodily, environmental load of a surgeon and increase efficiency of MIS.

생약재로 이용되고 있는 활나물은 항암효과가 밝혀져 그 이용이 기대되나 종자발아가 불량하여 재배가 어려운 식물이다. 따라서 GA3, 저온, KNO3, acetone처리가 활나물의 종자발아에 미치는 영향을 구명하여 앞으로의 재배연구의 기초자료를 얻고자 본 연구를 실시하였던 바 그 결과를 다음과 같다. 1. 활나물 종자의 발아율은 GA3 처리시에는 0.1 mM에 12시간 암상태에서, 저온처리시에는 3℃에서 1주간 처리하는것에서 가장 높았다. 2. 발아율은 KNO3 처리시에는 400 mM에 6시간, acetone 처리시에는 200 mM에 6시간 처리하는 것에서 가장 높았으며, 이들 처리결과가 GA3 또는 저온처리의 최적결과보다 양호하였다. 3. 포장출현율은 200 mM의 acetone 용액에 6시간 처리하는 것보다 400 mM의 KNO3에 6시간 처리하는 것에서 높은 것으로 조사되어 활나물 종자처리는 KNO3를 이용하여 암상태에서 처리하는 것이 바람직한 처리방법으로 평가되었다.