Anthocyanin has antioxidant and radical-scavenging effects which may protect cells from oxidative damage and reduce risk of cardiovascular diseases and cancer. A new peanut variety “Heuksaeng”(Arachis hypogaea ssp. hypogaea L.) with dark purple peanut skin was developed at the Department of Southern Area Crop Science, NICS, in Milyang in 2014. This variety was developed from the crossing line between cultivar “Iksan 31” with short stem and erect plant type and “Iksan35” with large grain and purple skin. “Heuksaeng” which is semi erect Virginia plant type has 32cm of main stem length and 25 branch number per plant. This also show more resistant to late leaf spot, web blotch and lodging, compared with check variety “Daekwang”. Each pod has two grains with ellipse shape of purple testa and its yield components is composed of 60 mature pods of per plant, 69g of 100-seed weight, 77% of pod shelling ratio in the regional yield trials(RYT). For 3 year regional yield trials the average kernel yield of “Heuksaeng” had 4.25 MT/ha similar to that of check variety.
Its seed quality show 26.9% of crude protein and 46.0% of crude oil and 53.4% of oleate in fatty acid composition. Peanut skin of variety “Heuksang” consist of 2 kind of anthocyanin compounds such as 4.67mg/100g of delpinidine-3-glucoside (D3G) and 1.18mg/100g of cyanidine-3-glucoside(C3G). Peanut variety with high anthocyanin conent in skin will be useful to the recent preference of colorful food with healthful functional compounds.
Peanut is grown worldwide in the tropics and temperate zones primarily as an oilseed crop (38-54%) and protein source(25-30%). A new peanut variety “Daan”(Arachis hypogaea ssp. fastigiata L.) with the high yield potential was developed at the Department of Southern Area Crop Science, NICS, in Milyang in 2014. This was developed from the crossing line between cultivar “Sangpyeong” with short stem and high quality and “Dakwang” with large grain. “Daan” which is Shinpung plant type has 44cm of main stem length and 13 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 34 mature pods of per plant, 127g of 100-seed weight, 75% of pod shelling ratio in the regional yield trials(RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety also showed more resistant to early leaf spot, late leaf spot, web blotch, stem rot and lodging compared with check variety “Daekwang”.
In the regional yield trials “Daan” outyielded check variety by 16% with 5.00 MT/ha for kernel yield.
To define whole genome-level of structural variation by ionization energies and radiation doses in plant, the seeds of Ilpum rice cultivar were acutely irradiated with gamma rays (100Gy, 200Gy, and 400Gy) and ion-beams (20Gy, 40Gy, and 80Gy), respectively. Six M1 rice plants were re-sequenced by Hi-Seq2500 with Ilpum cultivar as control. The average sequencing coverage of the individuals was 10.6X, and the average mapping rate to the rice reference genome (IRGSP-1.0) sequence was 96.95%. The individual plants were irradiated with gamma-400Gy and ion-50Gy had highest variation of SNP with 471,837 and 469,147, respectively. The number of insertion/deletion was 77,500 and 77,106, the synonymous and frame-shift were 7,859 and 7,763 in above two individuals. Although high genome variation shown between Ilpum cultivar and irradiated individuals, there were non-correlation between number of variation and radiation doses. However, five individuals, except ion-20Gy, showed 33 common variant blocks (CVBs) spanning 6 Mb in whole rice genome (1.6%). The CVBs were distributed on 12 rice chromosomes, Chromosome 6 had biggest CVB (5 blocks, 1.3Mb), whereas chromosome 9 had smallest CVB (0.01Mb). Total five hundred fifty one genes were in CVBs which can regard radiation sensitive genes or may be regarded as radiation hot spots in rice genome. This study will contribute to the improvement of the radiation mutation breeding research in genetic and genomic aspect.
Clubroot is a devastating disease caused by Plasmodiophora brassicae and results in severe losses of yield and quality in Brassica crops including Brassica oleracea. Therefore, it is important to identify resistance gene for CR disease and apply it to breeding of Brassica crops. In this study, we applied genotyping-by-sequencing (GBS) technique to construct high resolution genetic map and mapping of clubroot resistance (CR) genes. A total of 18,187 GBS markers were identified between two parent lines resistant and susceptible to the disease, of which 4,103 markers were genotyped in all 78 F2 plants generated from crossing of both parent lines. The markers were clustered into nine linkage groups spanning 879.9 cM, generating high resolution genetic map enough to refine reported reference genome of cabbage. In addition, through QTL analysis using 78 F2:3 progenies and mapping based on the genetic map, two and single major QTLs were identified for resistance of race 2 and race 9 of P. brassicae, respectively. These QTLs did not show collinearity with CR loci found in Chinese cabbage (Brassica rapa) but roughly overlapped with CR loci identified in cabbage for resistance to race 4. Taken together, genetic map and QTLs obtained in this study will provide valuable information to improve reference genome and clubroot resistance in cabbage.
본 논문은 전후 초기 일본의 홋카이도(北海道)에서 이루어진 소련점령지 반환을 위한 대중들의 초기 운동에 대하여 주안점을 둔 것이다. 운동의 기원, 그 뒤에 숨은 배경, 그리고 일본 전체 국민들의 광범위한 동조를 얻는데 실패한 이유에 대해서 연구하였다. 초기 대중들의 요구는 지방의 경제적인 욕구에 의해서 형성되었고, 또한 공범위한 국내적, 국제적인 정치적 발전이 반영되었다. 이런 운동에 참여하고 있던 여러 조직단체들은 그들의 요구가 직접적으로 구성원들의 물질적 이익을 반영한다고 믿고 단결하였다. 이 운동을 이끈 이념은 1960년대 말 중앙정부가 주도한 이후 영토회복주의자들을 이끌게 된 비물질적인 민족주의와는 분명히 거리가 멀었다. 또한 광범위한 대중을 동원하는 운동으로 나아가지 못했고 당시 일본에서 주도된 주권 정체성과 공명을 이루지 못하고 구조화에 실패하였다. 본 연구에서는 이 과정을 추적하였다.
Magnoliae Flos (Sini in Korean) is one of the most important oriental medicinal plants. In the Korean Herbal Pharmacopeia, the bud of the all species in Manolia denudate and Manolia genus were regarded as the botanical sources for ‘Sini’. Most the dried bud of Manolia denudata, Manolia biondii and Manolia sprengeri were used as ‘Xin-yi’ in China. Therefore, the purpose of this study was to determine and compare the ‘Magnolia’ species, four species including Manolia denudata, M. biondii, M. liliiflora and M. Kobus were analysis of sequencing data revealed DNA polymorphisms. The based on tRNA coding leucine/phenylalanine (trnL-F) and NADH-plastoquinone oxidoreductase subunit 5 (ndhF) sequences in chloroplast DNA. For the identification of ‘Magnolia’ species, polymerase chain reaction (PCR) analysis of chloroplast DNA regions such as ndhF have proven an appropriate method. A single nucleotide polymorphism (SNP) has been identified between genuine “Sini” and their fraudulent and misuse. Specific PCR primers were designed from this polymorphic site within the sequence data, and were used to detect true plants via multiplex PCR.
Alpiniae officinarum Rhizoma (the rhizome of Alpinia officinarum Hance, known as lesser galangal), a family of Zingiberaceae, has been used to reduce pain of infection and inflammatory diseases in Asian countries. The present study was focused to evaluate the inhibitory degranulation effect of Alpiniae officinarum Rhizoma extract in RBL-2H3 rat basophilic leukemia cells. Cell viability was measured by MTT assay. RBL-2H3 cells were stimulated by phorbol 12-myristate 13-acetate and calcium ionophore A23187. Mast cell degranulation was analyzed by measuring release of β -hexosaminidase in RBL-2H3 cell. Gene expression was measured by qRT-PCR and signaling molecules were detected by immunoblotting. The Alpiniae officinarum Rhizoma extract suppressed β-hexosaminidase release in dose-dependent manner and inhibited cycloxygenase-2 and tumor necrosis factor-α gene expression. Furthermore, it was found that Alpiniae officinarum Rhizoma extract reduced mitogen-activated protein kinases, especially phosphorylated p38, at 0.75 ㎎/㎖ of Alpiniae officinarum Rhizoma extract concentrations. These data show that Alpiniae officinarum Rhizoma extract has immunosuppressive effect in mast cell induced allergic inflammation.
In sustainable viticulture, green manure represents a safe and non-polluting way to bring large quantities of organic matter into the soil. The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. This study was conducted to evaluate the effects of green manure fertilization on yield and quality of the Campbell Early grape variety in the organic vineyard. Hairy vetch was the more productive green manure crop, yielding higher dry materials. Average yields of grape were significantly greater in hairy vetch + rye (13.02 ton ha-1) than nature weed (11.65 ton ha-1), respectively. The concentrations of total as well as individual anthocyanins were consistently higher with hairy vetch treatment compared with rye and nature weed, thus making the green manure cultivation is an environmentally friendly cultivation to increase the yield and anthocyanin contents in organic grape.
본 연구는 3,7-diarylpyrazolo [1,5-α]pyrimidines의 효과적인 one-pot regioselective 합성을 보여준다. 더욱이, 그 유도체는 뛰어난 CB1R 저해 활성을 나타냈다. 3,7-position에 diaryl group이 치환된 pyrazolo [1,5-α]pyrimidine은 CB1R 후보로서 가능성 있는 pharmacophore이다.
한국은 2050년까지 주요 선진국 중에서 고령화가 가장 심각한 사회로 전환되게 될 것으로 예상된다. 기대여명의 증가와 저 출산은 고령화를 더욱 악화시키며, 이는 심각한 사회문제로 발전하게 될 것이다. 이와 같은 문제를 해결하기 위해 한국정부는 2008년에 도시지역에는 주택연금제도를 도입하였으며, 2011년에는 세계 최초로 농촌지역을 대상으로 농지연금제도를 도입하였다. 그렇지만 이와 같은 제도는 설계 당시부터 복지상품이라기 보다는 장기적으로 손실과 수익의 균형에 초점을 둔 금융상품으로 개발되어 실질적으로 노인들에게 크게 인기를 얻지 못하였다. 따라서 본 연구는 농지연금제도를 활성화시켜, 농촌노인들에게 보다 더 많은 혜택을 주기 위해 지역 토지시장을 감안하여 지역별 농지가격상승률을 예측하고 연금액을 산출하였다. 또, 지금까지 사용한 년 혹은 분기별 감정가 대신에 월별, 지역별 실거래 가격을 모형에 적용하여 지역 토지시장, 고령화 수준 등 지역 여건에 부합하는 연금액을 산출하였다. 할인율자료도 가장 안정적인 3년 만기 국고채 수익률을 활용하여 미래농지가격을 예측하고, 이를 유동화하여 월 생활자금으로 지급되도록 하였다. 특히 농지규모가 가장 많고, 고령화 정도가 심각하여 농지연금의 잠재적 수요가 가장 높을 것으로 예상되는 경상북도와 전라남도를 사례지역으로 선정하고, 이를 전국평균과 비교하여 지역적인 차이도 함께 분석하였다. 이를 위해 농지가격 및 이자율 시계열 자료의 안정성을 검정하고, 장기농지가격을 예측하였다. 이를 활용하여 경북, 전남, 전국의 노인들의 월평균 지급액을 추정하였다. 분석결과 정책의 잠재적 수요가 가장 높은 두 지역이 가장 낮은 금액이 지급되는 것으로 추정되어 이는 또 다른 지역불균형을 초래할 수 있는 것으로 평가되었다.
Torrefaction is a treatment which serves to improve the properties of biomass in relation to thermochemical processing techniques for energy generation. In this study, the torrefaction of sewage sludge, which is a non-lignocellulosic waste was investigated in a horizontal tubular reactor under nitrogen flow at temperature ranging from 150-600℃. The torrefied sewage sludge products were characterized in terms of their energy yield, ash content and volatile fraction. The energy and mass yields decreased with an increase in the torrefaction temperature. The pelletization and the combustion characteristics of the torrefied sewage sludge was studied in isothermal and non-isothermal conditions.
Due to strong binding, optical clarity, adhesion to many surfaces, toughness and flexibility polyvinyl butyral(PVB) resin films are commonly used in the automotive and architectural application as a protective interlayer in the laminated glass. Worldwide million tons of PVB waste generated from end-of-life automotive associated with various environmental issues. Stringent environmental directive, higher land costs eliminate landfilling option, need a sustainable, environment-friendly technology to recycle these solid wastes. In our current study, we have developed a mechano-chemical separation process to separate PVB resins from glass and have characterized the separated PVB through various techniques, i.e., scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Feasibility for reuse of these recycled PVB has been investigated. The technology developed in our laboratory is sustainable, environment-friendly, techno-economical feasible process, and capable of mass production (recycling).
Hydrometallurgical process, which is one of the recycling method of EAFD in zinc, is a process to recover metallic crystal form that deposited on the cathod through electrolytic extraction by leaching valuable metals in appropriate solvent. Still, there is a lot of variable at electrowinning process, such as initial pH, space between electrode, voltage, current, cathod, or anode, Thus, many studies have been conducted previously. In this study, the consideration of optimistic condition in electrowinning process to recover the valuable metal in EAFD have been carried. The extraction amount by changing pH shows increase according to high initial pH, also, the alteration of extraction amount by controling the space between electrode, voltage or current has been measured. The difference of efficiency represent by decrease of electromotive force, sudden loss of voltage under specific condition, and using platinum as electrode shows most stable extraction. Furthermore, when distance between electrode are too close, the extracted zinc contact with anode, which make recovery difficult. The analysis result of zinc metallic crystal which been recovered draws spherical and cylindrical shapes are scattered and mixed, and consequence of EDX, content of zinc appear about 60%.
The Korean Solar Radio Burst Locator (KSRBL) is a solar radio spectrograph observing the broad frequency range from 0.245 to 18 GHz with the capability of locating wideband gyrosynchrotron bursts. Due to the characteristics of a spiral feed, the beam center varies in a spiral pattern with frequency, making a modulation pattern over the wideband spectrum. After a calibration process, we obtained dynamic spectra consistent with the Nobeyama Radio Polarimeter (NoRP). We compared and analyzed the locations of bursts observed by KSRBL with results from the Nobeyama Radioheliograph (NoRH) and Atmospheric Imaging Assembly (AIA). As a result, we found that the KSRBL provides the ability to locate flaring sources on the Sun within around 2′.
Today, in the course of globalization and crisis, the world needs the new ideas, new variants and ways of development of civilizations. Due to this, the author has developed and introduced own research (know-how) without world`s analogue. The main purpose of this research is to transfer Turkestan in the spiritual center (mega polis) of the international level. For the first time in history, as an example of one region, two large cardinal problems had been connected, i.e. on the one hand spiritual and cultural development of the society, on the other hand new 6th technological way to show to the world opportunity of Kazakhstan new model of spiritual-innovative development. After realization of this research the status of Kazakhstan will strengthen at international level. This workout is devoted to all people, especially to the youth - as future of our country.
One of the best solutions for the deficiency of clean water, especially for developing countries, is rainwater disinfection. In the past decades a lot of studies have been made to develop photocatalytic processes using TiO2 determining the performance on their surface oriented photocatalysis. However, most of these researches failed to consider the economical aspect as well as the effectiveness on the disinfection to antibiotic resistance genes. On the other hand, due to the issues of climate change and increased impermeable layer in urban area, flooding prevention is the best solution in water management systems. To remedy these two problems, a roof-harvested rainwater storage system was designed. In addition, a breakthrough technique using a solar simulator with self-rotating TiO2 nanotubes, to apply a photocatalytic system in disinfecting storage rainwater harvested from roof, was established.
Roof-harvested storage rainwater was analysed for TN, TP, SS and COD. Aside from these parameters, Escherichia coli (with multidrug resistant pB10 plasmid) was added to the sample. Samples were injected to the self-rotating TiO2 nanotube reactor system with exposure time of 0 to 360 min and 7 different setups. Results show that the developed system has increased disinfection properties compared to negative samples, though the presence of antibiotic resistant bacteria.
Present day, 1.1 billion people face the lack of water; 1.3 billion people cannot access modern energy and 1.02 billion people are still starving. Meanwhile, the number of human population keeps increasing and this will induce the water, energy, and food scarcity in the future even worse. Increasing the stock or finding new alternative resources of water, energy and food individually is the most practical way to avoid the scarcity. Behind this improvement, it must be realized that these resources are connected each other. Water needs energy to be treated and distributed; in opposite way energy production requires water for cooling system or as the sources in hydropower plant. Food is in needed water and energy for growing, producing and delivering. Food can also become new energy source from biofuel but the food intensification will deteriorate water quality. Therefore, the integrated development and management, known as Water-Energy-Food (WEF) Nexus, is needed to reach the balance and optimal development.
Fulfilling the human need faces another challenge when the climate change influences the availability of these resources. Many researchers report that climate change causes increase in the earth temperature alters the rainfall pattern and magnitude which affect the safe water availability. Further, it can affect the food and energy production. Taking an inappropriate use of water, energy and food will threaten the amount of resources itself. As it is a complex and complicated interconnection, it is difficult to determine the appropriate uses solely based on human judgment. Many researchers have developed computer models to simulate the correlation between each element and aid the decision maker. Due to its complexity, most of models only focus on one or two elements such as water-energy or water-food and put other element as the secondary parameter. This study provides overview of the WEF Nexus and inter-connections among the three elements. Furthermore, we will envision a development of an evaluation model that can simulate and optimize the use of water, energy and food efficiently under the climate change condition. If succeed, this model could be a decision support system for government or stakeholder to implement a regulation related to efficient use of water, energy and food.