양송이버섯에 발생하는 긴수염버섯파리에 대한 곤충병원성선충의 방제효과를 조사하였다. 양송이버섯에 발생하는 긴수염버섯파리 유충의 볏짚배지와 상토에서 30×30㎡ 내 밀도는 각각 평균 0.8마리와 22.2마리로 상토에서 월등히 높았다. 볏짚배지에 상토를 복토하고 첫관주 이후 2일간격 3회 관주시 곤충병원성 선충을 마지막 관주시 물대신 1회 곤충병원성선충을 살포한 시험구에서는 ㎡당 2.5×105 농도가 6.0×104 농도보다 약간 높은 방제효과를 보였고 2회 살포한 시험구에서는 살포 후 14일째에 각각 96.8%와 66.7%로 ㎡당 2.5×105 농도에서 높은 방제효과를 보였다. 따라서, 양송이버섯재배시 벗짚배지에 상토를 복토한 후 2일 간격 3회 관주처리 할 때 물대신 2일간격 2회 곤충병원성선충을 ㎡ 당 2.5×105 농도로 처리할 경우, 긴수염버섯파리 유충을 효과적으로 방제할 수 있을 것으로 판단된다.

The organism evaluation equipment (OEE) was developed to determine the insecticidal effect of atmospheric-pressure plasma jets (APPJ). The equipment consisted of a plasma-generating acrylic chamber (PGAC) equipped with a plasma generator, a plasma-maintaining acrylic chamber (PMAC), and plasma efficacy evaluation container (PEEC). The amount of reactive oxygen species (ROS) directly exposed to PEEC in the PMAC was measured using an ozone calculator. The ROS concentration in the PGAC and PEEC increased over time, and the optimum position for the PEEC was selected showing the lowest variance of ROS concentration. Based on the established equipment, five major insect pests (Aphis gossypii, Bemisia tabaci, Helicoverpa armigera, Tetranychus kanzawai, and Thrips palmi) were tested, and their knockdown time, recovery time, and median lethal time (LT50) were determined. As a result, B. tabaci showed the fastest knockdown in respond to the plasma, but the remaining insect pests were knock downed within 2-3 min. Recovery was the fastest in T. palmi and T. kanzawai and slowest in B. tabaci. The LT50 were determined as 13 and 16 min in B. tabaci and T. palmi, respectively. However, the LT50 of other insect pests showed over 21 min. In conclusion, the OEE system can be used to measure the optimum exposure time of plasma against various insect pests, and the plasma might be used as an alternative tools for pest control in the future.

최근 변화하는 기후로 인해 외래해충의 유입이 증가하였으며 발달된 교통수단 등으로 인해 새로운 지역으로 확산이 가속화되는 추세(Bale, 2002)이므로 그 중요성이 증대하고 있다. 본 연구는 우리나라의 최근에 침입한 대표적인 흡즙성 해충인 미국선녀벌레를 대상으로 충청지역의 발생분포를 파악하기 위해 수행되었다. 이를 위해 2018년 5월부터 8월까지 충남북 관내 주요 행정구역을 대상으로 미국선녀벌레 발생여부와 피해정도를 중심으로 조사하였다.

2015년부터 2016년 동안 우리나라 공항에서는 수입검역과정에서 금지식물로 폐기된 실적이 17만건에 달하며 그중 인천국제공항은 12만건 이상으로 가장 높다(농림축산검역본부 PIS, 2016). 인천국제공항을 통해 반입되는 휴대 금지식물에서 금지해충(과실파리류, 나방류 등) 유입 가능성이 높음에도 불구하고, 금지식물에서 발생하는 해충에 대한 객관적인 정보가 부족한 실점이다. 이에 2018년 1월부터 2018년 6월까지 인천공항으로 반입되는 휴대식물 및 해충조사를 실시하였다. 총 27,948건의 휴대식물에서 검사를 진행하였으며 98국가에서 316 품목을 조사하였다. 그 결과, 91건의 휴대식물에서 해충이 검출되었으며, 익은 바나나 14건, 망고스틴12건, 구아바 12건, 망고 11건, 람부탄 9건, 슈가애플 8건, 파일애플 4건, 고추 3건 등에서 검출되었다. 반입국가는 베트남이 47건으로 가장 많았으며 태국, 필리핀, 인도네시아 순이였다. 검출해충은 깍지벌레과와 가루깍지벌레과가 포함된 노린재목이 76건으로 가장 많았으며, 파리목은 11건이며 그중 오리엔탈과실파리가 1건이고, 개미류가 8건을 차지하였다.

본 연구는 산림, 하천, 자연 녹지 등 자연생태환경과 격리된 도심 속의 생태적 패치를 분석하고 이해하여 생태적 가치가 높은 생태 환경을 조성하는 방안을 모색해 보고자 실시하였다. 분석 대상지역은 도심 속의 생태적 패치로 다양한 입지 조건으로 조성되어 활용되고 있는 국립과천과학관(경기도 과천시 상하벌로 110일원) 일대를 중심으로 선정하였다. 생태적 패치(patch) 입지별 4개의 구역으로 구분하였고 각 조사 구역 별 결과에서 곤충상은 Ⅰ구역에서 61과 135종, Ⅱ구역에서 31과 46종, Ⅲ구역에서 31과 42종, Ⅳ구역에서 13과 14종 등으로 구분되었다. 전체 조사구역의 군집 분석 결과, 다양성 지수 4.30, 종풍부도 22.74, 균등도 0.84 등으로 구분되었다. 조사 결과, 수환경과 키 작은 수목을 중심으로 하고 경작 및 원예가 재배되는 Ⅰ구역에서 종 조성이 다양하게 분포하였고 소규모 생태적패치를 조성할 경우 수환경과 원예화원, 소규모 경작지 등이 분포할때 종 다양성이 높고 생태적으로 안정된 양상이 될 것으로 기대된다.

파리목(Diptera) 집파리과(Muscidae)에 속하는 토마토어리줄기집파리(Atherigona orientalis)는 열대·아열대 지역에서 분포하며 고추, 토마토 등 기주 특이성이 없어 여러 식물에 흔히 발견되는 해충이다. 현재까지 우리나라 작물에 피해는 보고되지 않았으나 국외 사례로 볼 때 우리나라 작물에서도 큰 피해를 유발할 것으로 예상되어 식물방역법상 관리해충으로 지정하여 관리하였다. 2016년 경상북도 의 성군에서 첫 국내 분포가 보고되었다. 우리는 이 종의 지역별 분포 현황 등을 확인하기 위하여, 2017년 4월부터 10월까지 전국 각지에 트랩을 설치하여 분포 조사를 실시하였다. 조사결과 총 2,601개체가 전국적으로 조사되어 국내에 널리 분포하고 있음이 확인되었다.

In 2017, a total of 82 non-target species representing six orders of insects were captured in four types of tephritid fruit fly (Diptera: Tephritidae) surveillance traps located in Korea; the species included 6 families of Coleoptera, 1 of Dictyoptera, 17 of Diptera, 2 of Hemiptera, 3 of Hymenoptera, 1 of Neuroptera, 4 of Lepidoptera, and 1 of Raphidioptera. Of these, Diptera were the most abundant; the main families included Muscidae, Tephritidae, and Anthomyiidae. Herein, based on a survey, we present a list of the species of non-target insects captured in the tephritid fruit fly surveillance traps.

기후변화는 전지구적 현상이며 온난화를 주패턴으로 하며 이상기상 현상의 빈도가 높아지는 등 기후예측불확실성이 증대로 이어지고 있다. 이에 따라 농업생태계의 기후변화에 대한 적응과 변화를 지표하기 위한 수단으로 말벌류 지표종을 선발하였다. 기후변화지표종 선발을 위한 지표의 객관성을 높이기 위해 지표 수준별로 개량화하였으며, 이를 통해 장수말벌, 등검은말벌, 털보말벌, 황말벌 4종을 지표종으로 선발하였다. 이 종의 장기 모니터링을 위한 무인모니터링 시스템을 개발하였다. 이를 통해 전국적 모니터링 네트워크 등의 구축이 가능할 것으로 판단된다.

Dasineura jujubifolia was introduced into Korea for the first time in 2011, a gall midge feeding on jujube (Ziziphus jujuba Miller (Rhamnaceae)). Since then the jujube gall midge become a serious insect pest of Jujube in Korea. In this year, we surveyed regulated and alien insect pests by collecting damaged leaf samples from different regions in Korea. Jujube gall midge and its life stage were identified by morphological and genetic characteristics. For molecular identification, we analyzed the cytochrome c oxidase subunit I (COI) sequences of pest. In this study, we report the morphological description and genetic information of D. jujubifolia.

돌발해충인 갈색날개매미충, 미국선녀벌레, 꽃매미, 갈색여치, 먹노린재, 목화진딧물과 남방계해충인 애멸구, 배추좀나방, 볼록총채벌레, 담배거세미나방, 풀색노린재를 대상으로 충청과 제주지역에서 발생실태를 조사한 결과, 농경지내 갈색여치, 목화진딧물은 화학약제의 사용으로 관찰이 쉽지 않았고 풀색노린재는 생태습성성상 관찰이 쉽지않아 이들 해충을 제외하고 발생실태를 조사하였다. 갈색날 개매미충은 충청지역의 경우 2017년까지는 집중분포 양상을 보이다 2018년 알조사에서부터는 임의분포를 보이기 시작하였고, 미국선녀벌레는 경기와 충북 지역과 인접한 곳에서 집중분포를 보였다. 제주지역의 경우 2017년 제주시 한림읍 협재리 인근에서 최초 발생하였다. 먹노린재는 2018년 최대발생양상을 보였고, 충남 서천, 청양, 홍성, 태안을 중심으로 대발생 양상을 보였다. 애멸구는 충남 공주와 서천에서 밀도가 높았다. 갈색날개매미충과 미국선녀벌레의 발생밀도는 낮게 조사되었으나 발생면적은 증가하였다. 볼록총채벌레는 충청지역 감나무에서 발견되었고 제주지역 감귤에서는 발견되지 않았으나 녹차밭에서는 발생량이 높았다. 추후 돌발 및 남방계 해충에 대한 위해성 평가를 통하여 발생가능성 정보를 제공할 계획이다.

The tribe Centistini is a cosmopolitan group, comprising 94 species (Yu et al., 2016; Aguirre et al., 2017). Among them 44 species have been known from the East Palaearctic region. Centistini are known to be solitary koinobiont endoparasitoids of final instar larvae (rarely) and Coleopteran adults, particularly the families Anthicidae, Carabidae, Chrysomelidae, Coccinellidae, Curculionidae, and Staphylinidae (Loan, 1964; 1972; Shaw, 1985; Chen and Achterberg, 1997). Previously, 16 species of 1 genera of tribe Centistini, have been recorded from Korea. In the present study, a total 19 species of 2 genera of the subfamily Euphorinae is recognized from Korea. Among them, three species, Allurus liturature (Haliday), Centistes minutus Chen & Achterberg and C. sylvicola Belokobylskij are reported for the first time from Korea.

The domestic natural enemy industry was formed by public institutions before the natural enemy companies were formed. In 1995, during the decentralization period, the technololgy of natural enemy breeding developed by the Rural Development Administration and the Agricultural Research Institute of each province were spread to the Agricultural Technology Centers of each county, Agricultural Technology Centers has supplied a large number of natural enemies to farmers for free. Since the beginning of the 2000s, when the first natural enemy company called Korea-IPM was created in Korea, the natural enemy industry boomed with the birth of Cecil Co., Ltd., a large natural enemy company. Prior to the birth of a natural enemy company, Domestic methods of using nautral enemies to release the natural enemies against the target pests and to test their effectiveness were similar to those of using chemicals. After then, the introduction of banker plant, a natural enemy protection plant, began to develop the concept of natural enemy protection limited to Aphidius colemani, and the effect was excellent. However, there were many cases where the success and failure of biological control were staggered because only the use value of the natural enemy for the target pest was dealt with. Therefore, in our laboratory, we are exploring and developing of the using method of trap plant that can concentrate densities by attracting insect pests. Also, when using a variety of natural enemies in a single plant, we are studying strategies to increase the pest control efficiency by identifying the interraction between natural enemies and determining their amount of releasing.

The potato tuber moth (PTM) is an oligophagous pest on solanaceous crops. We tested adult olfactory behavior and larval development on 5 cultivated tomato varieties leaves including Moneymaker, Campari, Ailsa craig, LA3475 and E6203, and one wild species, S. pimpinellifolium. Bioassay on PTM larvae showed highest developmental performances in Ailsa craig, and lowest in LA3475. Tomato leaf hydro-distilled oils of Moneymaker, Campari, Ailsa craig, S. pimpinellifolium and E6203 showed strong attractiveness and LA3475 exhibited repulsiveness for mated adult PTM of both sexes in two-armed bioassay. The hydro-distilled oils of all tomato leaves contain the fatty acid n-hexadecanoic acid as a major compound. We conformed presence of n-hexandecanoic acid (palmitic acid) in the leaves by GC-MS. LA3475 consists of (1-hexyltetraecyl) cyclohexane (10.5%) as the second major compound which was absent in all other tomato varieties. A standard sample palmitic acid was tested against the pest at 0.1, 1 and 10 mg/ml did not show any significant olfactometer effect. On other hand, phytol showed a weak attractiveness (60.7-63.6%) and (57.6-60.5%) for male and female PTM, respectively at 0.1, 1 and 10 mg/ml. Additionally, SEM of these tomato varieties leaves showed they have trichomes with distinct shapes. We are working on HPLC, and GC-MS analysis of specific chemicals in trichomes of these tomato varieties leaves.

Residual contact vial (RCV) method was used to monitor insecticide resistance in field populations of the melon thrips, Thrips palmi. Median lethal doses (LD50) at 8 h post-treatment of six insecticides (chlorfenapyr, cyantraniliprole, cypermethrin, dinotefuran, emamectin benzoate and spinosad), which are commonly used for T. palmi control, were determined at 8 h post-treatment using a susceptible RDA strain. The diagnostic doses for on-site resistance monitoring of the six insecticides, which were determined as two-fold higher doses of LD90 for the RDA strain, were in the range of 0.299 to 164,25 μg-1cm2. To test the applicability of RCV for T. palmi, insecticide resistance levels in three field populations (Gyeonggi; GG_AS, Chungbuk; CB_CJ, Jeonbuk; JB_KJ) were evaluated. Field populations showed reduced mortality (0-50% mortality) to spinosad, cypermethrin, cyantraniliprole and emamectin benzoate, that they have different degree of resistances to these insecticides. In particular, all test field populations exhibited 0% mortality to spinosad, suggesting wide spread of spinosad resistance in the field. Moreover, no detectable mortality to emamectin benzoate was observed in JB_KJ strain, suggesting uneven distribution of emamectin benzoate resistant population of T. palmi. To provide more precise information on resistance profiles and distribution in T. palmi populations, it would be necessary to conduct a large scale resistance mapping for broad geographical regions.

Small hive beetles (SHBs) are parasites of bee colonies and is one of the invasive pest species worldwide. In this study, we aimed to investigate a chemical responsible for the attractiveness of a hive to this pest. Based on the laboratory observation, we found the fermented pollen dough (honey bee food) was highly attractive to adult SHBs. Olfactometer bioassay showed the crude methanol extract of this material is attractive to both sexes (> 60%). However, the methanol extracts of fresh honey and pollen patty which we used as a food of SHB were not attractive to the pest. The crude methanol extract of fermented pollen dough was subjected to partitioning with n-hexane, chloroform, ethyl acetate, and n-butanol. The n-hexane and chloroform fractions were attractive > 80% for both sex SHBs. On the other hand, ethyl acetate and n-butanol were repulsive >70% and >80%, respectively. The wandering larvae of the pest showed a reverse response for the solvent fractions observed in adults. HPLC and GC-MS analysis indicated the methanol extract of fermented pollen dough constitute butanoic acid (7.70%), neopentylamine (7.29%), (E)-9-octadecenoic acid ethyl ester (3.89%) and hexadecanoic acid, ethyl ester. While these compounds were not found even in a trace level in the methanol extract of honey and pollen patty. Based on this findings these constituents of the fermented pollen dough were formed by the action of SHB and its larvae and we believe these volatile organic compounds are responsible for attractiveness to SHB as pollen dough undergoes attach by SHB and ferment. We are working on investigating attractiveness of each of these chemicals toward the pest. We recommend further work on these chemicals for their application to control this pest.

Laodelphax striatellus is an important pest of rice due to not only sucking rice seedlings, but also transmitting serious plant viruses. Among various kinds of insecticide groups (carbamates, organophosphorus, neonicotinoids, etc.), carbofuran, a systemic carbamate insecticide, has been most extensively used to control rice pests including L. striatellus, resulting in widespread carbamate resistance in Korea and other Northeast Asia countries. To identify the genes associated with carbofuran resistance, we obtained a 14-fold higher resistant strain (SEL9) from the mixed-field population (SEL0) by consecutive selection. A transcriptome-based analysis was conducted and differentially expressed genes (DEG) were compared between the SEL9 and SEL0 strains. A total of 96,185,150 reads were analyzed, of which 62,860,430 reads were mapped. From these reads, 15,356 transcripts were annotated. A total of 327 up-regulated and 275 down-regulated genes were identified in the resistant SEL9 strain compared to SEL0 strain by DEG analysis. Gene ontology (GO) analysis was performed using DEGs showing statistical significance (P < 0.05). The GO analysis identified 1,320 genes in biological process group, 1,100 genes in cellular component group and 428 genes in molecular_function group.

The Japanese pine bast scale, Matsucoccus matsumurae (Kuwana, 1905), is a destructive pest of pine trees in America, East Asia and Northern Europe. The spread of damage to black pine trees, Pinus thunbergii, of M. matsumurae was reported from all southern, and some eastern and west costal regions in Korea, under the name of M. thunbergianae which was newly described by Miller & Park (1987) as a new species. Historically, M. thunbergianae was synonymized with M. matsumurae by Booth & Gullan (2006), on the basis of molecular sequence data. However, the supporting data for the synonym is unavailable in any DNA database, such as GenBank and BOLD. Moreover, M. thunbergianae have been still used to the resent scientific studies in Korea. Here, we performed morphological and molecular comparison to review the result of Booth & Gullan (2006), using M. matsumurae from Fukuoka, Japan and topotype materials of M. thunbergianae from Goheung, Korea. Our data supported the opinion of Booth & Gullan (2006) following results: The morphological features of adult female and male of M. thunbergianae are identical to those of M. matsumurae. Also, DNA sequences (COI, 18S rDNA and 28S rDNA) of M. thunbergianae showed identical or very low genetic distances with those of M. matsumurae. From the regional sampling in Korea, M. matsumurae was newly found in Jeju and Seoul.

Hymenoptera is one of four largest orders of insects, with over 150,000 described species, 132 families, 27 superfamilies, and 2 suborders. There are currently about 3,000 hymenopteran species in 65 families and 20 superfamilies known in South Korea, of which the majority of the families have been studied while some of them are still poorly studied. The present overview on South Korean Hymenoptera aims to provide brief taxonomic history of the studies, a complete bibliography, a list of South Korean hymenopteran species, and information on South Korean hymenopterists for each taxon.

The black pine bast scale, Matsucoccus thunbergianae Miller et Park, is one of the severe pest to pine forests in South Korea. In 1963, a severe pine damage by unknown causes were reported at Goheung region and it turned out that the damage was caused by the black pine bast scale, M. thunbergianae 20 years later. The black pine bast scale have been causing serious damage to Japanese black pine forests in the east and southwestern coastal area of South Korea and its distribution has been expanded. The areas of Japanese black pine forests damaged by M. thunbergianae was 4,043ha in 41 cities or districts in 2017. In males, two nymphal instars are followed by wingless preadult, the pupa, and the adult, whereas the female adult directly emerges from the second nymphal instar. According to recent sex pheromone survey for male adult, M. thunbergianae occur mainly single generation in South Korea with possibility its phenology was advanced probably due to climate change. Until now, the most effective measure to control the black pine bast scale is to trunk injection with insecticides after thinning of a damaged pine forest.

The control of crop diseases still largely relies on spray applications of standard synthetic fungicides. As a result, the development of fungicide resistance by pathogens is a major limiting factor for the control of diseases. In this paper, the recent outbreak of practical resistance to fungicides including sterol demethylation inhibitor (DMI), Quinone outside inhibitor (QoI), and succinate dehydrogenase inhibitor (SDHI) fungicides as well as the progress of related research will be discussed. To combat resistance development, the Research Committee on Fungicide Resistance, the Phytopathological Society of Japan, was established in 1991 and has been conducting several activities. The Committee made a guideline indicating how to use at-risk fungicides. The occurrence of fungicide resistance may rather increase in the future as the choice of fungicides is often difficult due to the lack of effective alternatives. Development and integration of disease management tools such as disease-resistant or -tolerant cultivars and non-fungitoxic disease resistance inducers need to be accelerated more rapidly not only to dissolve the problem of fungicide resistance but also to reply public concerns about agrochemicals.