Sympathetic innervation stimulates β-adrenergic receptors, triggering cyclic adenosine monophosphate (cAMP) production and enhancing protein secretion in salivary gland cells. While cAMP signaling, in conjunction with Ca2+ signaling, is essential for salivary gland function, the identified cAMP-producing G-protein-coupled receptors (GPCRs) remains limited. Here, we report the presence of cAMP-producing adenosine receptors in salivary gland cells. By reanalyzing publicly available single-cell transcriptome datasets of human and mouse submandibular glands, we identified mRNA expression of adenosine A1, A2A, A2B, and A3 receptors. Additionally, we confirmed that 5’-N-ethylcarboxamidoadenosine (NECA), an adenosine A2B receptor agonist, increases cAMP levels in human salivary gland cells, suggesting a physiological role for adenosine A2B receptors. Our findings enhance understanding of adenosine’s regulatory function in salivary glands and highlight new avenues for research on cAMPproducing adenosine receptors.

목적 : 본 연구는 근시에서 굴스트란드 모형안에 편심 렌즈를 적용했을 때의 광학적 효과를 분석하고자 하였다. 방법 : 굴스트란드 모형안은 3D 시뮬레이션 프로그램을 사용하여 정밀하게 설계하였으며, 근축 근사 없이 정확 한 분석을 제공하는 광선 추적 기술을 적용하여 편심 렌즈로 인한 초점거리와 굴절력의 변화를 정밀하게 조사하 였다. 결과 : 렌즈 중심축의 편심이 초점거리, 굴절력, 광 경로 차이에 미치는 영향을 다양한 편심 조건에서 분석하였 으며, 특히 중심축으로부터 일정 높이에서 평행광선이 입사하는 경우를 집중적으로 조사하였다. 광선의 입사 높이 가 극도로 커짐에 따라 편심의 효과는 감소하고, 출사 광선의 초점은 서로 가까운 지점으로 위치하는 것이 확인되 었다. 렌즈와 눈의 굴절력과 편심에 따른 프리즘 굴절력 효과를 포함하는 전체 굴절력은 광선의 입사 높이가 감소 함에 따라 크게 변화하는 것으로 나타났다. 결론 : 본 연구 결과는 사시 및 사위와 같은 시각적 이상을 교정하기 위해서, 의도적으로 편심된 렌즈를 설계하 고 처방하는 데 유용한 정보를 제공할 수 있을 것으로 기대된다. 또한, 편심 렌즈 설계 시 발생할 수 있는 비선형적 광학 효과를 최소화하여 사용자에게 최적의 시각적 성능 제공을 보장할 수 있을 것으로 기대된다.

Maize (Zea mays. L) is one of the major sources of green fodder for livestock in Pakistan. Crop management plays a key role in obtaining high yields for green fodder. Fertilizer application, seed rate, and row spacing are critical components of crop management, which can significantly affect crop biomass. To determine the best production technology, a two-year (2021-2023) study was conducted at the research area of National Agricultural Research Center, Islamabad. Plant height, number of leaves, leaf area, green fodder yield per acre, and green fodder yield per hectare were recorded. Various row spacing (15 cm, 30 cm, 45 cm, and 60 cm), fertilizer ratio (N: P = 55:30, 65:40, 75:50, and 85:60), and seed rates (30 kg/ac, 35 kg/ac, 40 kg/ac, and 45 kg/ac) were applied. Results obtained experiments revealed that in both growing seasons, the maximum green fodder yield was obtained when fertilizer N: P ratio was 75:50 (green fodder biomass: 74.61 t/ha and 72.56 t/ha). Similarly, the optimal seed rate was found to be 40 kg/ac, which resulted in the highest green fodder yield (73.41 t/ha and 72.88 t/ha in two seasons). Furthermore, the plant of maize at row spacing of 30 cm was found to generate the maximum green fodder yield (72.39 t/ha and 72.40 t/ha, respectively). Green fodder yield per hectare was found to be positively correlated with plant height, number of leaves, and leaf area. These findings underscore the significance of applying a fertilizer ratio of N: P = 75:50, a seed rate 40 kg/ac, and a row spacing of 45 cm for higher yields of green fodder in maize crop.

최근 국내에서는 온라인 거래를 통한 화훼류의 판매가 증가 하고 있다. 온라인 플랫폼의 종류에 따라서 각기 다양한 운송 시스템과 포장 방법을 이용하고 있지만, 품질과 관련된 정보가 부족한 실정이다. 특히, 일반 택배 배송을 이용하는 직거래 방식 은 다양한 외부 환경에 노출되기 때문에 품질에 문제가 발생할 수 있다. 절화장미는 외부의 환경에 노출되는 통기 구멍이 있는 포장 박스와 외부의 환경과 단절된 밀폐된 포장 박스가 사용되 고 있다. 포장 박스의 종류에 따른 절화장미의 품질을 조사한 결과 통기구가 있는 포장 박스를 이용하여 택배 운송한 절화장 미는 운송 중의 낮은 습도로 인해 생체중 감소가 높아 품질이 하락하였다. 밀폐된 포장 박스를 이용해서 운송한 절화장미는 통기 구멍이 있는 포장 박스를 이용해 운송한 절화장미보다 생 체중 감소는 작았지만, 포장 박스 내부의 온도가 높아져 품질이 하락하였다. 내부가 코팅된 밀폐형 포장박스 내부에 절화장미와 함께 냉매를 동봉하여 포장 후 운송하면, 운송 중 포장박스 내부 의 온도를 경감하고 품질을 보존하는 효과가 있었다.

Gentamicin is an aminoglycoside antibiotic effective against aerobic gram-negative bacteria and is also used in veterinary medicine, particularly in the swine and bovine industries. However, no gentamicin product is currently approved for treating equine diseases in Korea. The present study aims to examine the time-dependent residue of gentamicin in horses after intravenous injection (IV) via jugular vein. The test product was injected at 6.6 mg/kg BW via jugular vein in nine horses. Blood was collected from the horse's jugular vein at 15 minutes, 30 minutes, 1, 4, 8, 12, 24 and 48 hours after injection. To purify the gentamicin in serum, 100μL of 20 mM HFBA in DW, 100 μL of 30% trichloroacetic acid and 300 μL of 20 mM heptafluorobutyric acid (HFBA) in acetonitrile (ACN) were added to 500 μL of serum and supernatant was applied to LC-MS/MS after centrifugation. LC-MS/MS-8050 analyzed the level of gentamicin in serum with Electrospray ionization (ESI) and multiple reaction monitoring (MRM) positive mode. Gentamicin C1 was 478 m/z and product ions were 322, 157 m/z. Precursor ion of Gentamicin C1a was 450 m/z and product ions were 322, 160 m/z. Precursor ion of Gentamicin C2 and C2a was 464 m/z and product ions were 322, 160 m/z. The LC column was a C18 and mobile phase composed of 20 mM HFBA in 5% ACN and 20 mM HFBA in 50% ACN. The amount of gentamicin was calculated by adding four components of gentamicin (C1, C1a, C2 and C2a). The pharmacokinetic parameters of gentamicin were calculated by the WinNonlin program. The Cmax of gentamicin in horse serum was 93 ± 17 μg/kg and the Tmax was 0.25 ± 0 hours. The T1/2 was 6.41 ± 2.32 hours and the CLt was 0.05 ± 0.01L/hr/kg. The Vd was shown as 0.44 ± 0.13 L/kg and the MRT was 1.98 ± 0.55 hours. In conclusion, our data provides useful pharmacokinetic parameters for gentamicin in horses following IV injection.

The swallowing reflex is modulated by multiple sensory inputs, such as bolus volume, viscosity, and taste. The interactions among different types of sensory information have been extensively studied. However, the influence of oral temperature on bolus volume perception has not been investigated. The aim of this study was to assess the effect of temperature on volume perception sensitivity in healthy individuals. Five volumes (5, 10, 15, 20, and 25 mL) of distilled water were estimated at three different temperatures, 4℃ (cold), 21℃ (room temperature), and 45℃ (warm), using a visual analogue scale. There were no significant differences in the sensitivity of oral volume perception across temperatures. These findings suggest that the ability to perceive bolus volume remains stable under temperature variation.

This experiment was carried out to study the effect of elapsed time after air flow cutoff on the germination rate of Italian ryegrass seed with different moisture contents during natural drying on reclaimed land, Jangheung and Kimje of Korea from 2023 to 2024, respectively. Seeds with moisture contents of 15.3, 22.3 and 28.0% were placed in vinyl bag (30 × 40 cm) at storage thicknesses of 10, 15, and 20 cm, and air flow was cutoff for 48 h. Seed moisture content, seed temperature (℃) and germination rate were investigated at 12-h intervals. After 48 h of airflow cutoff during natural drying, seed moisture content did not significantly differ among storage thickness treatment (p>0.05). When Italian ryegrass seeds with moisture contents of 27~28% were stored under conditions with air flow cutoff at 15~20cm thickness for 48 h, the seed temperature reached up to 30℃ and the germination rate was excellent at around 70~80%.

This study aims to develop an AI-based analysis system that aligns with the international trend of AI legislation, including the EU's AI Act, while also addressing the analytical needs of the public sector. The focus is on providing timely and objective information to policymakers and specialized researchers by exploring advanced analytical methodologies. As the complexity and volume of data rapidly increase in the modern policy environment, these methods have become essential for governments to obtain the objective information needed for critical decision-making. To achieve this, the study integrates machine learning, natural language processing (NLP), and Large Language Models (LLM) to create a system capable of meeting the analytical demands of government entities. The target dataset consists of “quantum” field data collected from South Korea's National R&D Information System (NTIS). Machine learning was applied to this data to assess the validity of the analysis, while BERTopic, a natural language analysis package, was used for text analysis. With the introduction of LLMs, the extracted information from machine learning and natural language analysis was not merely listed but also connected in meaningful ways to provide policy insights. This approach enhanced the transparency and reliability of AI analysis, minimizing potential errors or distortions in the data analysis process. In conclusion, this study emphasizes the development of a system that enables rapid and accurate information provision while maintaining compatibility with international AI regulations such as the AI Act. The use of LLMs, in particular, contributed to enhancing the system’s capabilities for deeper and more multifaceted analysis.

This study explored factors affecting variability in second language (L2) learning motivation among Korean university students and how they appraised their L2 learning experience. In this study, 85 undergraduate students majoring in English or English education from three universities in Seoul, South Korea, reflected on their past English learning experience. They drew a motigraph and wrote a retrospective reflection essay covering their English learning that spanned over ten years. Researchers divided participants into two groups: a high variability (HV) group and a low variability (LV) group. Data were analyzed using open, axial, and selective coding. Findings suggest thncontextual factors such as the learning method, atmosphere, and situation were main (de)motivational factors for the HV group. In contrast, the LV group was (de)motivated by intrapersonal factors, including the learning context appraisal. These results imply that visualizing long and short-term goals and positive appraisal of the L2 learning experience can help L2 learners maintain a stable pattern in L2 learning motivation.

목적 : COVID-19 팬데믹 기간 동안 사회적 거리두기와 비대면 활동 증가로 인해 생활습관이 크게 변화하였다. 본 연구는 근거리 작업시간, 수면시간, 스트레스 수준의 변화가 비정시(근시, 원시, 난시) 발생에 미치는 영향을 분 석하고자 하였다. 방법 : 본 연구는 질병관리청 국민건강영양조사 제8기(2019~2021년도) 조사 중 2020년에 참여한 대상자들 중 만 40세 이상인 성인들을 대상으로 수행된 단면 연구이다. 연구 대상자는 안검진을 받은 성인 중 안과 질환이 없는 2,564명을 포함하였다. 굴절 이상은 등가구면굴절력을 기준으로 하였다. 결과 : 근거리 작업시간이 하루 4시간 이상인 그룹에서 근시 유병률이 유의하게 증가하였다. 반면, 1시간 이하 의 근거리 작업을 수행하는 그룹에서는 원시 유병률이 높았다(p<0.0001). 수면시간이 6~8시간인 그룹에서 근시 유병률이 가장 높았으며, 원시는 6시간 미만 그룹에서 높았다(p=0.0082). 스트레스 수준이 높은 그룹에서 근시 유 병률이 유의하게 증가하는 경향을 보였으나, 다중 로지스틱 회귀분석에서는 근거리 작업시간이 짧은 그룹에서 난시 유병률이 더 높은 경향을 보였으며(p<0.0001), 수면시간과 스트레스의 영향은 통계적으로 유의하지 않았다. 결론 : 본 연구 결과, 근거리 작업시간 증가가 근시 발생에 가장 큰 영향을 미치는 요인임이 확인되었으며, 수면 패턴과 스트레스 또한 비정시와 연관성을 가질 수 있음을 시사하였다. 이는 COVID-19 팬데믹과 같은 환경적 변 화가 시력 건강에 미치는 영향을 이해하고, 근시 예방 및 관리 전략을 수립하는 데 기여할 수 있다.

인공관절 치환술 환자의 합병증 진단을 위한 자기공명영상 검사에서 발생한 금속 인공물을 감소시키는 VAT(view angle tilting) 기법과 딥러닝 알고리즘 중 K-공간 기반의 deep resolve(boost, sharp, DR)를 적용하여 그 유용성 에 대해 평가하고자 하였다. 자체 제작 팬텀과 3T 장비로 일반적인 VAT, DR이 적용된 VAT로 T1 강조영상, T2 강조영상, 단시간 반복 회전 연쇄기법(short tau inversion recovery, STIR) 영상들을 병렬영상 가속계수 2, 3, 4를 적용하여 획득하였다. 획득된 영상에서 왜곡도, 팬텀 바닥에서 금속 인공물까지 거리, 신호대잡음비를 정량적 평가하였고, 영상 품질은 정성적 평가하였다. 왜곡도는 일반적인 VAT와 DR이 적용된 VAT의 T1 강조영상, T2 강 조영상, STIR 모두 유의한 차이가 없었다(p>0.05). 또한 금속 인공물까지 거리도 T1 강조영상, T2 강조영상, STIR 모두 유의한 차이가 없었다(p>0.05). 신호대잡음비는 일반적인 VAT보다 DR이 적용된 VAT의 가속계수 4에서 T1 강조영상은 103%, T2 강조영상은 85.2%, STIR은 73.3% 최대로 증가하였고, 영상품질 평가점수는 T1 강조영상은 5점, T2 강조영상은 4.6점, STIR은 4.8점으로 가장 좋았다. 본 연구를 통해 VAT 기법의 영상품질 저하를 DR 기술 로 개선할 수 있었다. 금속 인공물이 발생했을 때 DR이 적용된 VAT의 가속계수를 4로 적용한다면, 검사시간을 단 축하면서 보다 진단적 가치가 높은 영상을 획득할 수 있을 것으로 사료된다.

산마늘(Allium microdictyon)과 울릉산마늘(A. ulleungense) 은 수선화과(Amaryllidaceae) 부추속(Allium)에 속하는 다년 초 식물로 산마늘은 우리나라에서는 지리산, 오대산 등의 고산 지대, 울릉산마늘은 울릉도에 분포하고 있다. 본 연구는 산마늘 과 울릉산마늘의 휴면과 발아특성을 조사하여 효과적인 대량증 식법을 위한 기초자료를 제공하고자 수행하였다. 실험은 2021 년 7월에 강원도 정선에서 재배한 산마늘 종자와 2021년 8월에 울릉도에서 채종한 종자를 사용하였다. 수분흡수 실험결과, 산 마늘과 울릉산마늘 모두 수분흡수 3시간 만에 20% 이상의 수분 흡수율을 보여 물리적 휴면이 없는 것으로 판단하였다. 온도 처리(25/15, 20/10, 15/6, 5℃) 실험에서 패트리디쉬에 종자 를 치상 후 30일 이전에 5℃를 제외한 나머지 온도 처리에서 모두 발아가 나타났으며, 산마늘과 울릉산마늘 종자의 발아 적 온은 20~25℃인 것으로 확인되었다. 저온층적(0, 4, 8, 12주)처 리 결과, 산마늘과 울릉산마늘 종자 모두 발아율 향상에는 큰 효과가 없었지만, 저온층적처리 기간이 길어질수록 발아세가 증가하는 양상을 보였다. GA3 처리 결과, 산마늘 종자는 처리 농도 간 유의성이 나타나지 않았지만, 울릉산마늘 종자는 처리 농도가 높아질수록 평균발아일수 및 발아균일도에서 유의적인 차이를 나타냈다. 이러한 결과를 통하여 산마늘과 울릉산마늘 종자는 non-deep PD 유형인 것으로 판단된다.

Lumpy Skin Disease (LSD) and Foot-and-Mouth Disease (FMD) cause substantial economic losses on the livestock industry. Therefore, vaccinations have been implemented as the control strategy in endemic countries. However, the potential adverse effects of administering vaccines for both diseases simultaneously have not been thoroughly evaluated. The aim of this study was to assess the impact of vaccinating dairy cows with either or both LSD and FMD vaccines on milk production and physiological parameters such as milk temperature, rumination time and body weight. The experimental groups were divided into four according to the injection materials: 1) saline, 2) LSD vaccine, 3) FMD vaccine, and 4) both vaccines. The impact of vaccination on milk yield and physiological parameters was evaluated daily until 12 days post-vaccination, and milk components were analyzed twice, once per week. Among the experimental groups as well as each vaccine group, no statistically significant differences (p < 0.05) were observed at milk yield, milk components, or milk temperature. This suggests that simultaneous vaccination of LSD and FMD can be administered without adverse effects.

Visfatin, an adipokine secreted by cells, is crucial for intracellular nicotinamide adenine dinucleotide+ biosynthesis. Extracellularly, visfatin plays diverse roles in inflammatory conditions, including obesity, which is closely linked to osteoclastogenesis. We previously showed that visfatin enhances receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in bone marrow-derived macrophages. However, its enzymatic activity during this process is poorly understood. Here, we investigated visfatin’s effects on RANKL-induced osteoclast differentiation. Our results demonstrate that visfatin promotes this differentiation, an effect inhibited by FK866, an inhibitor of visfatin’s enzymatic activity. Furthermore, FK866 also inhibited RANKL-induced osteoclast differentiation. These findings suggest that inhibiting visfatin’s enzymatic activity modulates osteoclast differentiation. Thus, visfatin plays an important role in osteoclastogenesis, both intracellularly and extracellularly, and FK866 has therapeutic potential for diseases characterized by imbalanced osteoclast formation, such as osteoporosis and periodontitis.

This experiment was carried out to evaluate the effect of natural drying methods on the moisture content and germination of Italian ryegrass seed in Jicheon reclaimed land, Jangheung, Korea, from 2023 to 2024. The natural drying methods tested included seed spread thicknesses of 2.5, 5.0 and 7.5 cm; seed reversal frequencies of 1, 2 and 3 times per day; and two reversal methods: seed-only reversal and complete mixing of seed with a dry mat. The thinner seed spread thickness significantly accelerated moisture content reduction(p<0.001). However, the reduction in moisture contents due to the number and method of seed reversal was minimal, approximately 1%. Seeds initially having around 48.9% moisture content required 2, 4 and 5 days to reach a moisture content of 14% when dried at spread thickness of 2.5, 5.0 and 7.5 cm, respectively. Seeds with an initial moisture content of 23-26% required 1 to 2 days to reach 14% moisture content while maintaining approximately 80% germination rate under the tested natural drying methods. When seeds with an initial moisture content of 48.9% were dried at a spread thickness of 2.5 cm, the germination rate was 74.6%, which was significantly lower than the 83.8 and 81.6% germination rates observed for seeds dried at 5.0 and 7.5 cm thickness, respectively(p<0.002). These results suggest that for faster drying and higher-quality Italian ryegrass seed, harvesting at 30% seed moisture content, utilizing the cultivation field as a seed drying place, and applying a seed spread thickness of 2.5 to 5.0 cm are optimal practices.

Effective cooling strategies are critical for cultivating high-quality ornamental plants during the summer. The fan-and-pad cooling system reduces greenhouse temperatures by drawing air through wet pads, which humidify and cool the air, aided by fans on the opposite side. However, the paper-based pads (corrugated cellulose) used in this system have limited durability and degrade with prolonged use. Nanocomposite hydrogels, with their polymer-based structure, can absorb and retain moisture through swelling, presenting a promising alternative. This study examines the application of nanocomposite hydrogels, focusing on their hygroscopic properties and cooling efficiency under various temperatures and wind speeds. When treated with lithium chloride solutions at 25%, 50%, 75%, and 100% saturation, higher LiCl concentrations reduced weight but increased swelling capacity. Optimal cooling effects were achieved with wind speeds of 1.0 m/s at 25°C and 1.5 m/s at 35°C, with greater efficiency observed at lower wind speeds. These findings suggest that integrating nanocomposite hydrogels into cooling pads could enhance durability and reduce maintenance compared with conventional paper pads.

This study examined impact of agricultural cooperative membership on technical efficiency, income, and costs of coffee farmers in Southern Shan State, Myanmar. Using stochastic frontier analysis (SFA) and propensity score matching (PSM) methods, this study estimated average treatment effect on the treated of agricultural cooperative participation. Results showed that technical efficiency, total income, and total variable costs were significantly higher for cooperative members than for comparable non-members. However, net income of members is not significantly different from that of comparable non-members.

가위벌과에 속하는 Coelioxys의미기록 2종 Coelioxys conoidea Illiger, 1806 와 Coelioxys formosicola Strand, 1913을 한국에서 처음으로 보 고한다. 본 종의 기재, 분포정보, 그리고 삽화를 제공한다.

목적 : 근시 어린이를 대상으로 2년 동안 OK-렌즈 착용자의 근시 감소에 대한 효능을 평가하고자 하였다. 방법 : OK-렌즈 그룹은 OK 렌즈 착용 기준에 부합하는 30명의 대상자로 구성되었고, 단초점 안경을 착용한 대조군에는 31명이 포함되었다. 이 연구에 사용된 OK-렌즈는 CRT였고, 굴절이상 측정은 조절마비제 사용없이 자 동굴절검사기와 자각적 굴절검사를 통하여 이루어졌다. 결과 : 총 61명(OK-렌즈 그룹과 대조군은 각각 30명, 31명)의 대상자가 2년 동안의 검사를 완료했다. 연구 초 기의 OK-렌즈군과 대조군의 평균 연령±SD는 각각 10.13±1.81세와 10.32±1.73세였고, 등가구면굴절력은 OK-렌즈군에서 –2.53±0.96 D, 대조군에서 -2.56±1.10 D로 두 그룹 간 유의미한 차이는 없었다. 2년 동안 등 가구면굴절력의 증가는 OK-렌즈군과 대조군에서 각각 –0.70±0.56 D, -1.27±0.78 D였으며, 그 차이는 통계적 으로 유의한 차이가 있었다(p<0.001). 결론 : OK-렌즈 치료는 학령기 어린이의 근시 진행을 늦추는 데 도움이 될 수 있다.

As part of the 2024 research initiative, “Investigation and Discovery of Prokaryotes in Freshwater Systems,” samples were collected from diverse freshwater habitats, including both water and soil environments. Approximately 2,000 bacterial strains were isolated as single colonies and identified through 16S rRNA gene sequence analysis. Among these, 38 strains shared ≥98.7% 16S rRNA gene sequence similarities with those of known bacterial species not previously reported in Korea. These strains were thus categorized as newly recorded bacterial species in Korea. These 38 bacterial strains displayed significant phylogenetic diversities, spanning 2 phyla, 4 classes, 15 orders, 24 families, and 34 genera. These unrecorded species were classified into the following classes: Actinomycetia (with genera including Microcella, Conyzicola, Curtobacterium, Leucobacter, Microbacterium, Frigoribacterium, Lysinibacter, Streptomyces, Nonomuraea, Actinocorallia, Ruania, and Actinoplanes), Alphaproteobacteria (Paracoccus, Youngimonas, Loktanella, Corticibacterium, Neorhizobium, Onobrychidicola, Ferranicluibacter, Aureimonas, Asticcacaulis, and Novosphingobium), Betaproteobacteria (Rhodoferax, Rugamonas, and Cupriavidus), and Gammaproteobacteria (Rheinheimera, Shewanella, Kosakonia, Leclercia, Hafnia, Yersinia, Pseudomonas, Lysobacter, and Acinetobacter ). Further characterization included assessment of Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic relationships. This report presents detailed phylogenetic and phenotypic characteristics of these bacterial species.