This study was done to evaluate the susceptibility, systemic effect, residual effect and control effect in each developmental stages of biotype Q of sweetpotato whitefly against insecticides, acetamiprid+spinetoram SC and dinotefuran SG. Two insecticides were showed similar activity against the eggs, and showed higher activity in acetamiprid+spinetoram SC against the nymph and adult. In systemic effect, two insecticides have a similar activity. It was showed higher activity in root zone systemic application than leaf zone systemic application. Residual effect was showed higher in acetamiprid+spinetoram SC (92%) than dinotefuran SG (44 %) at seven days after treatment. Control effect was showed all over 90 % activity at tree- and seven days after treatment. Therefore, these insecticides are expected to control the sweetpotato whitefly effectively.

This study was carried out to evaluate toxicities of two formulations (microemulsion, ME and wettable powder, WP) for acetamiprid+etofenprox against aphids (Aphis gossypii), thrips (Frankliniella occidentalis) on the cucumber greenhouse and whiteflies (Trialeurodes vaporariorum and Bemisia tabaci Qbiotype) on the tomato greenhouse. The activities of two formulations were evaluated by testing residual effect and toxicities against eggs, nymphs and adults of whiteflies in the laboratory, and control effect in the greenhouse against whiteflies, aphids and thrips. All trials were tested at the recommended concentration(RC) and a half concentration of RC of each formulation. In the laboratory trials against eggs and nymphs of whiteflies, the effect of ME was more effective than that of WP and showed more than 80% ovicidal activities, and 40%~60% larvicidal activities on the 3rd instars nymph of whiteflies at the recommended and its half concentrations. On the residual effect against the adults of the two whitefly species, WP formulation was more effective than ME and showed more than 70% mortality at the 5th day after treatments of recommended concentrations. In the field trials, the effects of ME against whiteflies and thrips were more excellent than those of WP. On the other hand, the effect of WP against aphids was more excellent. These results indicate that the formulation of insecticide can be played an important role in the control strategy of the pest in field.

DNA-based technology are about to revolutionize the analysis of population structures as well as the determination of individual indentities. Furthermore, the analysis of polymorphic DNA regions make it possible to reach detailed conclusions on family relationships of individual. Microsatellite loci are increasingly used in population genetic and evolutionary studies. Simple sequence repeats (SSRs) or microsatellites consisted of short tandem repeats (usually 1-6 nucleotide) have shown advantages over other markers. We report here the isolation and characterization of nine highly polymerphic microsatellite loci for phylogenetic and population genetic use in silkworm. Comparative analysis of diverse silkworm strains with microsatellite locus revealed several alleles and discriminative heterozygosity values. A list of primer sequences that tag each locus is provided. The usefulness of microsatellite markers can be expected to enhance the classification in silkworm.

We analyzed fault plane solutions of the recent twenty-two earthquakes which occurred from 2004 to 2006 in the central part of the Korean Peninsula by using P- and S-wave polarities along with SH/P amplitude ratios. The fault plane solution shows that strike-slip fault is dominant here, especially for the events with local magnitude equal to or greater than 3.0. However, some events with local magnitude less than 3.0 show normal fault or strike-slip fault with normal components. In the case of strike-slip fault, its orientation is almost in the direction of NNE-SSW to NE-SW almost parallel to the general trend of faults, while the compressional axis of the stress field trends ENE to E-W. The result is almost consistent with the stress field in and around the Korean peninsula, as reported previously. We cannot give any appropriate explanations to the normal faulting events along the western offshore and inland areas whether it is related to the local stress changes or tectonically unidentified extensional structures. Thus, an extension of investigations is desirable to clarify the cause of such phenomena.

In this study, alterations in whole proteome expression patterns in the head of Japanese medaka (Oryzias latipes) was investigated following acute or chronic exposure to benzo(a)pyrene (BaP) (25 μg L-1) for 48 hrs and 15 days, respectively. The

Changes in stress-associated biomolecules can be used as an important criterion for assessing the levels of environmental pollution because living organisms demonstrate contamination-stimulated stress responses. This study was conducted to determine the e

This study compared the pregnancy rates of Korean native donor cattle after either a timed artificial insemination (TAI) or embryo transfer (TET) following the synchronization of ovulation using a controlled internal drug release (CIDR) device together with estradiol benzoate (EB) and prostaglandin (). Fifty five cows and 8 heifers which had been previously used for embryo production were assigned to two treatments: (1) Thirty-two cattle received a CIDR device and 2 mg EB (Day 0), 25 mg injection at the time of CIDR removal on Day 7, and 1 mg EB injection on Day 8. All of the cattle received a TAI 30 h (Day 9) after the second EB injection (TAI group). (2) Thirty-one cattle received the same hormonal treatments as in the TAI group. The cattle with corpus luteum (CL) received a TET on Day 16 using frozen-thawed embryos (TET group). Ultrasonographic observations demonstrated that the proportion of cattle with synchronized ovulation on Day 10 and the concomitant formation of new CL on Day 13 did not differ between groups (p>0.05); the overall mean rates were 65.1 and 73.0%, respectively. The conception and pregnancy rates did not differ (p>0.05) between the TAI (12.5% and 12.5%) and TET groups (13.0% and 9.7%), respectively. We conclude that the pregnancy rate following TAI or TET in Korean native donor cattle was poor, which might be due in part to a poor synchrony of ovulation and concomitant CL formation.

A B. thuringiensis kurstaki was first discovered by H. Dulmage in 1970 and commercialized as a DipelTM due to powerful toxicity to various Lepidoptera. Previously we isolated B. thuringiensis kurstaki on the basis of plasmid DNA profiling and H-antigen serotyping. The aims of this study were to screen larvicidal activities and select the highly active B. thuringiensis isolates against the important polyphagous pests of mandarine oranges and vegetables. The colony forming unit (CFU/ml) of each of culture mixtures was determined to estimate the δ-endotoxin concentration. The bioassay against artificial diet-rearing insects was conducted by surface contamination methods using the Petri dishes specially designed and manufactured by SPL Lifesciences. The insecticidal activities to the natural diet-rearing insect were measured by the application of spore and crystal mixtures to the leaf discs of the chinese cabbage with Potter spray tower. The following insects were used for the larvicidal activities of B. thuringiensis isolates: beet armyworm Spodoptera exigua, diamondback moth Plutella xylostella, giant looper Ascotis selenaria, tobacco cutworm Spodoptera litura, and variegated cutworm Peridroma sucia.

Acute lymphoblastic leukemia (ALL), a predominantly pediatric disease involving uncontrolled proliferation of white blood cells within the bone marrow, is strongly associated with chromosomal translocations. The human chromosomal translocation t(12;21)(p12;q22) is the most frequently encountered chromosomal rearrangement in pediatric B-lineage ALL, and results in fusion of the TEL and RUNX1 genes. The resulting TEL/RUNX1 fusion protein is generally thought to be a transcriptional repressor that interferes with RUNX1-mediated transactivation. We used a luciferase assay system to investigate the effects of TEL/RUNX1 and PEBP2β on RUNX1-mediated transactivation. TEL/RUNX1 blocked the synergistic transactivation achieved by PEBP2β and RUNX1, and coimmunoprecipitation and immunofluorescence analyses showed that PEBP2β interacted with TEL/RUNX1 and was sequestered in the cytoplasm. Theses results suggest that TEL/RUNX1 inhibits RUNX1-mediated transactivtion via cytoplasmic sequestration of coactivator(s) such as PEBP2β.

The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), follicle stimulating hormone (FSH), luteinizing hormone (LH), and 1mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones. After 65h of maturation, oocytes were exposed to phosphate buffered saline (PBS) with 7% ethanol (v/v) for 7 minutes, and then the oocytes were washed and cultured in tissue culture medium (TCM) 199 containing 5 ug/ml cytochalasin B for 5h at in an atmosphere of 5% and 95% air with high humidity. After cytochalasin B treatment, the presumptive parthenotes were cultured in porcine zygote medium (PZM)-5 and cleavage of the parthenotes was assessed at 72h of activation, Normally cleaved parthenotes were cultured for an additional 8 days to evaluate their ability to develop to blastocyst and hatching stages. The fetal bovine serum (FBS) were added at Day 4 or 5 with concentrations of 2.5, 5 or 10%. The blastocyst rates were ranged within in each treatment. However hatching rate was dramatically decreased in non-addition group. In this experiment, embryo viability in female reproductive tract may be estimated before embryo transfer with in vitro culture adding FBS by hatching ability.

Here we analyze if the ionized shells associated with giant HII regions represent the progenitors of the larger neutral hydrogen supershells detected in the Milky Way and other spiral and dwarf irregular galaxies. We calculate the evolutionary tracks that 12 HII shells found by Relano et al. (2005, 2007) would have if they expanded into the interstellar medium because of multiple supernovae explosions occurring inside the cavity. We find, contrary to Relano et al. (2007), that the evolutionary tracks of these HII shells are inconsistent with the observed parameters of the largest and most massive neutral hydrogen supershells. Thus, an additional energy source to the multiple supernovae explosions is required in order to explain the origin of the most massive neutral hydrogen shells.

에너지원으로서 수소를 생산하기 위하여 하니컴 구조를 갖는 모노리스에 10 wt% Ni/CeO2-ZrO2 촉매를 담지한 후 메탄의 수증기 개질 실험을 수행하였다. 다른 CeO2/ZrO2 몰비를 갖는 촉매들 중에서, Ni/CeO2-ZrO2(CeO2/ZrO2=4/1)촉매가 700-800℃에서 높은 메탄의 전환율을 보여 주었다. 10wt% Ni/CeO2-ZrO2 촉매가 담지된 금속 모노리스 촉매체는 높은 열전도도와 비표면적들로 인하여 좋은 촉매 특성을 보여줌을 확인할 수 있었다. 또한, 금속모노리스 촉매체는 반응물에서 과다의 수증기에 의한 수소 수율에서 크게 영향을 받지 않음을 알 수 있었다. GHSV=30,000h-1, 반응물 비(H2O/CH4=3.0) 반응온도 800℃에서 금속모노리스 촉매체는 98%이상의 메탄의 전환율을 보여주었다. 생성물 가스에서 CO2/CO의 비는 수증기/메탄의 반응물비가 증가할수록 수성가스화 반응에 의하여 증가됨을 알 수 있었다.

전세계 주요 원자력선진국들의 사용후핵연료 처리에 대한 기술 및 정책현황을 알아보고 향후 우리나라의 연구방향을 제시해 보았다. 재처리 정책을 가진 소위 핵연료주기 국가들은 최근 선진핵 연료주기기술에 기초한 새로운 사용후핵 연료 관리정책을 발표하였다. 그 정책은 사용후핵연료 내에 함유된 우라늄 또는 초우란 원소들을 재순환하고 고독성의 방사성 물질 및 장반감기를 가진 물질들을 소멸하거나 단반감기 원소로 변환하는데 초점을 맞추고 있다. 이러한 정책은 원자력의 자원 활용성을 높일 뿐만 아니라, 영구 처분할 고준위폐기물의 양을 감소시켜 궁극적으로 원자력의 지속가능성을 높여 준다. PUREX 방법에 기초한 습식재처리를 우선순위로 선택한 대부분의 국가들은 이 습식방법이 건식방법에 비해 실용화에 앞서 있음을 그 선택 의 근거로 든다. 그러나 습식방법은 건식에 비해 핵확산저항성 측면에서 더욱 민감하다. 왜냐하면 이 습식방법은 약간의 공정수정에 의해 순수 플루토늄을 회수 할 수 있기 때문이다. 반면에 아직까지 실용화 단계까지는 도달해 있지 않지만 고온 용융염을 사용하는 Pyroprocess와 같은 건식처리 방법은 순수한 플루토늄을 회수 할 수 없어서 핵비확산성 측면에서 유리하며, 제4세대 원자로로 고려되는 고속로의 핵연료주기 등에도 여러 가지 이점을 가지고 있다. 따라서 우리나라의 경우 현재 이 Pyroprocess에 대한 연구가 활발히 진행되고 있다.

In this study, we examined the effects of egg yolk powder added to flour dough on the lipid oxidation of fried products during storage. The flour dough containing the egg yolk powder (0, 5, and 10%) was fried in sunflower oil at 180℃ for 90 sec. The fried products were then stored at 60℃ for 9 days in the dark. The lipid oxidation of the fried products was evaluated by fatty acid composition, peroxide values (POV), conjugated dienoic acid (CDA) contents, and thiobarbituric acid (TBA) values. The color and phospholipids (PL) contents of the fried products were also determined by colorimetry and high performance liquid chromatography, respectively. The addition of egg yolk powder to the dough decreased the POV, CDA contents, and TBA values of the fried products during storage. Although POV, CDA contents, and TBA values significantly increased in the products without egg yolk powder during storage, little change was observed in the products with egg yolk powder. The PL contents remained relatively constant in the fried products added with egg yolk powder during storage. The lightness and greenness of the fried products decreased, and the yellowness increased, as the storage time increased. The results clearly indicate that the addition of egg yolk powder to the dough improved the lipid oxidative stabiliy of the fried products during storage in the dark, and the PL in the egg yolk might have contributed to the improvements in lipid oxidative stability.