Based on a research proposal from Cambridge University that knowing the anatomical structure of the insects wouldhelp make an excellent biomimetic robot, we studied the anatomical characteristics of insects for application to jumpingrobots. We choose crickets, grasshoppers, and mole crickets among the Orthoptera. The external and muscle microstructuresof the three insects were compared using a stereomicroscope, an optical microscope and an electron microscope. Musclefiber bundles of the third legs of the grasshoppers and crickets were very dense, although their mitochondria numberswere smaller than their first leg. Muscle fibers of the first legs of the mole crickets are more loosely arranged than grasshoppersand crickets’ first legs, and there is a large number of different sized mitochondria, although the distinction between musclebundles is not clear.

The effects of Postharvest CO2 treatment on the quality and shelf life of oyster mushroom packaged with oriented polypropylene (OPP) film bag were investigated. On the day of harvest, the ‘Gonji-7ho’ oyster mushroom grown by bottle cultivation of Jangheung County, Korea transferred to a laboratory in Wanju County and were cooled in a cold room at 3°C for 1 day and then treated with 30% or 50% CO2 for 3 hours at 3°C. After the CO2 treatment, 400 g of oyster mushroom were sealed into 20 μm thick OPP film bag (width: 29 cm, length: 24 cm) used in the actual farmhouse. The package gas composition, hardness, color change, off-flavor index, browning index, and overall quality were evaluated during storage at room temperature (RT) for 6 days and at 3°C for 21 days. As a result, During storage at RT, the concentration of carbon dioxide in the bag of 30%, 50% CO2 were higher than untreated and shelf-life of oyster mushroom at 50% CO2 was reduced 1 day due to off-flavor while 30% CO2 or untreated was 2 days. During storage at 3°C, the concentration of carbon dioxide in the bag was kept low at 30%, 50% CO2 treatment compared to untreated, the respiration of oyster mushroom at 30, 50% CO2 were lower than untreated during initial 7 days storage at 3°C, but ethylene production were not different. The hardness of oyster mushroom at 30% CO2 was higher, the lightness (L* value) of stem surface was higher, the yellowness (b* value), browning index was lower and odor index was lower than untreated or 50% CO2. 30% CO2 treated oyster mushroom packaged with OPP film bag kept 4.2~16.2% O2 and 4.2~15.5% CO2 concentration in the bag during storage at 3°C, and showed highest overall quality index. Marketable shelf-life was assessed 10 days for untreated, 17 days for 30% CO2, and 16 days for 50% CO2, respectively.

Flammulina velutipes belonging to white rot fungi is one of the commercially important edible mushrooms and is produced in large quantities due to the introduction of a automated and mechanized cultivation system in Korea. Despite the chief item of export among edible mushrooms, Flammulina velutipes has the lowest distribution rate of domestic cultivar, estimated that about 20 percent. As the result that most white cultivars of Flammulina velutipes produced and exported in Korea were introduced from Japan, farmers pay a large amount of royalties. Therefore, we try to develop a new pure domestic cultivars as a substitute for Japanese cultivars. To breed both white and gold superior strains, we selected the crossing mother groups including 10 white strains of ASI 4198 etc. and 7 brown strains of ASI 4049 etc. and mated each of the 17 strains by mon-mon hybridization. 19 white and 14 brown strains were chosen through two selection experiment over 2014 2016. In the third selection experiment this year, we finally selected one white(Fv 16 c 37) and the other gold(Fv 15 a 31) strain. Two selected strains were cultivated in the same environmental conditions. Spawn running period on the sawdust substrate required 30days at 20°C. The cultivation period and optimum temperature were 12±1 days at 14°C for primordia formation, 5 days at 4°C for inhibition phase, and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in two selected strains and Megumi as a control Japanese cultivar harvested in optimal stage was as follows: 10.5±0.81mm and 139.7±4.23mm in Fv 16 c 37, 10.8±0.43mm and 128..2±7.31mm in Fv 15 a 31, and 10.9±0.41mm and 141.8±4.64mm in Megumi respectively. The Yield of Fv 16 c 37, Fv 15 a 31 and Megumi was 271.2±11.84g, 237.7±9.05g and 270.7±16.87g per 1100ml in bottle cultivation.

The Pleurotus tuoliensis is a mushroom named Chinese ‘Bai-Ling-Gu’ from species of Pleurotus nebrodensis. we are mated for selection of cytoplasmic hybrid by mitochondria microsatellite marker and the method of Mon-Mon mating between monokaryotic strains derived from Pleurotus ferula ASI 0629 (Beesan No.1) and Pleurotus tuoliensis ASI 0663 (Baekryung 20). The cytoplasmic hybrids were identified 8 strains contained nuclear DNA bands of 'Baekryung 20' and mitochondrial DNA of monokaryon strains derived from Pleurotus ferula ASI 0629 (beesan No.1). The KiMB-Plft-15-81 was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Baekwhang'. The 'Baekwhang' cab be grown without an extra after-ripening period, can utilize bottle cultivation of Pleurotus eryngii. And also the ‘Seolwon’ is similar to the existing Pleurotus tuoliensis in shape and physiological characteristics, formed high stipe. We therefore expect that this new strains will substitutional goods of Pleurotus eryngii.

There are three kinds of Pleurotus eryngii : P. eryngii var. eryngii (P. eryngii), P. eryngii var. ferula (P. ferula : A-Wei-Mo) P. eryngii var. nebrodensis (P. nebrodensis : Bai-Ling-Gu). We bred P. ferula using Di-Mon mating and physiological assay and selected as follow (1) ‘Beesan No.1’ produced high yields, (2) ‘Beesan No.2’ was excellent morphological shape and anti-adipogenic Activit, (3) ‘Ergo’ included high ergothioneine such as anto-oxidant material, (4) ‘GW10-45’ included highest ergothioneine such as anto-oxidant material, (5) ‘Maeksong’ included high ergothioneine and effect of anti-inflammatory. We suggest that there are able to food-medicine materials.

Volvariella volvacea is mainly cultivated in subtropics area like South-East Asia. Because it is cultivated in rice straws, it is called a straw mushroom. That mushroom grows well in high temperature about 30~38°C and high humidity. Straw mushroom is a homothallic mushroom, so it is difficult to identify whether the offspring is different from the parents. This study was carried out to investigate RAPD primers that can be used for identification the DNA polymorphism of Volvariella volvacea genetic resources. 9 strains were collected from various countries like China, Vietnam etc. When ITS regions of their DNA were analyzed, they proved to be Volvariella volvacea. A cultivation test was conducted to measure the morphological characteristics of them 2 strains, KMCC04380 and KMCC04382 were selected for breeding resources because the mycelium of them grew well on medium and fruiting bodies were formed quickly. The Universal PCR Fingerprinting kits(UPF primers) were used to confirm the genetic polymorphisms of the 2 strains. As a result of confirming the DNA bands, 2 of 12 primers could be used to genetically distinguish 2 strains. About 50 spores were isolated from their fruiting bodies respectively and they also will be confirmed DNA polymorphisms by using UPF primers.

The production scale of mushroom cultivation in Korea is approximately 600 billion won, which is 1.6% of the Korean gross agricultural output. Annually, ca. 190,000 tons of mushrooms are harvested in Korea. The oyster mushroom is one of the most favorite and commonly consumed mushrooms in Korea. In case of breeding, the protoplast fusion technique of the oyster mushroom, P. ostreatus was first commercialized in the world. To develop the high temperature varieties, various examinations were accomplished. Protoplast fusion of abalone mushroom, high temperature, and oyster mushroom, popular mushroom, were attempted. 2 strains of P.ostreatus and 2 strains of P.abalonus were fused to each other by protoplast isolation. Fusion strains were investigated by random amplified polymorphic DNA(RAPD) marker, and selected strains were cultivated at 25°C after completing the mycelial growth. As a result of sawdust bag cultivation, most of strains showed the fruiting body, but the morphological characteristics among them were not significant different. However, these protoplast fusion strains were expected as new parents strains to develop varieties.

A new commercial cultivar ‘GARES-HM-17-1’ of Hypsizigus marmoreus was developed by the method of mono-mono crossing between monokaryotic strains derived from Hae-Sal 3Ho. The parental strains are characterized by the property of high quality and a short period of time in cultivation. The optimum temperature of mycelial growth was 25°C and that of fruiting body development was 15~16°C. The period of harvesting including primordia formation was 23.0 days shorter than that of control strain Mangadak No. 2. The color of pileus and stipe surface was neutral-white, respectively. The yield was 82.0±5.8g/850cc plastic bottle. Analysis of the genetic characteristics of the new commercial cultivar ‘GARES-HM-17-1’ showed a different profile as that of the control strain, Mangadak No.2, when RAPD (Random Amplified Polymorphic DNA) primer was used. This new cultivar ‘GARES-HM-17-1’ of H. marmoreus is characterized by the improved quality and a short period of time in cultivation after scratching. It would be of much help to expand the cultivation area of H. marmoreus.

H. mamoreus is a mushroom with abundant favor and medicinal use. However, its cultivation has problems such as the long cultivation period, low biological efficiency and microbiological contamination, so new cultivars should be developed. In this study, 55 strains of H.marmoreus were analyzed with ISSR markers to identify precise genetic information in collected germplasm as mother strains in breeding. ISSR 13 and 15 markers were confirmed polymorphism. The three strains (KMC03106, KMC03107, and KMC03108) with white cap color were close genetic relationship in the UPGMA analysis of both the ISSR 13 and 15 markers. Especially in the PCR result of the ISSR 15, the collected germplasm were differentiated to three groups following collecting year. As these results, the ISSR 15 marker would be able to classify the phylogeny of cap color and genetic variation along the collecting year. Therefore ISSR markers will confer effective selection of mother strains for breeding of H. mamoreus.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called “smart agriculture” is changing the landscape of agriculture. Precision Agriculture’s core container production for the mushroom cultivation temperature, humidity, irradiation, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. The Lentinula edodes (shiitake) is an edible mushroom native to East Asia, which is cultivated and consumed in many Asian countries. It is considered a medicinal mushroom in some forms of traditional medicine. The fungus was first described scientifically as Agaricus edodes by Miles Joseph Berkeley in 1877. It was placed in the genus Lentinula by David Pegler in 1976. We controlled different light source (Blue-Red-White combined LED, blue LED, red LED and fluorescent light) with different intensity of LED irradiation (1.5, 10.5, 20.5 μmol/m2s for LEDs) to compare growth and development. Lights were treated with 12 hour on/ 12hour off cycle maintained in a controlled room with 19 ~ 21oC temperature, 80~90% humidity, and 1,000 ppm CO2 atmosphere concentration for 30 days. Growth and development differed from the LED color source and intensity of LED irradiation. Growth and development was most effective in 10.5 μmol/m2s for blue LED. All LED light sources showed less growth and development in lowest intensity of irradiation, which indicates that higher than 1.5 μmol/m2s for LED is not effective. After harvesting fruit bodies, we measured their weight and length, thickness of pileus and stipe, chromaticity, and hardness. 10.5 μmol/m2s blue LED group was the best result of harvest with average individual weight (24.7g) and length (61.98mm), thickness (29.93mm) of pileus and length (33.60mm), thickness (16.86mm) of stipe with fine chromaticity, hardness. This results show us that 10.5 μmol/m2s blue LED was the best effect on growth and development of Lentinus edodes (shiitake) mushroom’s ICT system container type environment.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

We experimented to see if the tolerance of Agaricus bisporus to Pseudomonas sp. causing the bacterial brown blotch disease which is causing great damage in the button mushroom cultivation was inherited. There was no correlation between mycelial growth rate and mushroom tolerance to pathogens at each temperature. In the mycelial stage, the strains tolerance to Pseudomonas sp. were not as strong as those of their parents, but they were generally stronger or more tolerance than those of weaker strain (ASI1321). In fruiting body, tolerance was decreased compared to mycelial. In fruiting body, later generations of two strains had similar tolerance, unlike strong or weak tolerance of parental generation. Therefore, browning of fruit body is thought to be caused not only by tolerance but also by various other factors. Especially, the future generation of the strains which were tolerance to Pseudomonas sp. were very weak in the mycelial stage, indicating that the tolerance of the parental generation was inherited to the later generations. The damage of each pathogen was different in mycelium and fruiting body. P. tolaasii caused higher browning than P. agarici in fruit body. P. reactans did not have a significant effect on the mycelium but affected the browning of the fruit bodies. P. agarici had higher mycelial growth inhibitory ability than fruiting body.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called “smart agriculture” is changing the landscape of agriculture. Precision Agriculture’s core container production for the mushroom cultivation temperature, humidity, irradiation, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. Auricularia auricula-judae, known as the Jew's ear, wood ear, jelly ear or by a number of other common names, is a species of edible Auriculariales fungus found worldwide. The fruiting body is distinguished by its noticeably ear-like shape and brown colouration. The fungus can be found throughout the year in temperate regions worldwide, where it grows upon both dead and living wood. We controlled different light source (Blue-Red-White combined LED, blue LED, red LED and fluorescent light) with different intensity of LED irradiation (1.5, 10.5 and 20.5 μmol/m2s for LEDs) to compare growth and development. Lights were treated with 12 hour on/ 12hour off cycle maintained in a controlled room with 19 ~ 21oC temperature, 85~95% humidity, and 1000ppm CO2 atmosphere concentration for 30 days. Growth and development differed from the LED color source and intensity of LED irradiation. Growth and development was most effective in 1.5 μmol/m2s irradiation for blue LED. All LED light sources showed less growth and development in highest intensity of irradiation, which indicates that higher than 20.5 μmol/m2s for LED is not effective. After harvesting fruit bodies, we measured their weight, length, width, thickness and chromaticity of fruit bodies. 1.5 μmol/m2s blue LED group was the best result of harvest with average individual weight (5.38g), length (65.37mm), width (56.87mm) and thickness (13.51mm) with fine chromaticity. This results show us that 1.5 μmol/m2s blue LED was the best effect on growth and development of Auricularia auricula-judae mushroom’s ICT system container type environment.

Polygonum multiflorum (Polygonaceae family) is effective to cholesterol lowering effect, increased immunity effect and whitening effect. P. multiflorum root is used for medicinal herb that can be used as raw material for food. In this study, acetic acid fermentation was adding extracts of P. multiflorum root (0%, 1%, 3%, 5%) for the developing healthy beverage. In this study, we investigated the contents of free sugars, organic acids and inorganic components of P. multiflorum root. As a result of free sugars from P. multiflorum root analysis, three kind of free sugars were determined the glucose, sucrose and lactose by HPLC. Four kind of organic acids were found in lactic acid fermented P. multiflorum root. And oxalic acid, lactic acid and succinic acid were detected in organic acids. The content of lactose and lactic acid in lactic acid fermented P. multiflorum root was the highest among the free sugars and organic acids. The content of minerals were in high orders of sodium > calcium > magnesium in P. multiflorum by acetic acid fermentation. The content of inorganic components was highest in lactic acid fermented solution with 5% and proportional to the content of P. multiflorum

The purpose of this study was to expand the utility of the Polygonum multiflorum. Also, we fermented P. multiflorum by mushroom mycelial, and analyzed for chemical compositions and biological active of fermented P. multiflorum root. The crude protein content did not show a significant difference between control and fermented P. multiflorum root, crude fat, ash and crude fiber content of fermented P. multiflorum root were lower than control. The content of soluble nitrogen free extract of P. multiflorum root was significantly higher than fermented P. multiflorum root. The major amino acids of P. multiflorum root were determined the arginine. The content of arginine and glutamic acid were 586.67 mg%, and 283.78 mg%, respectively. Sixteen kinds of amino acids were detected in fermented P. multiflorum root, and the major amino acids were determined the arginine and threonine. The total amino acid contents of control and fermented P. multiflorum root were 3,469.03 mg%, and 3,630 mg%, respectively. As the results of antioxidant activity test, the antioxidant capacity of fermented P. multiflorum had a higher than the control. As the mushroom fermentation progresses, it is confirmed that the amino acid content and antioxidant capacity were increased, and it is expected to develop the product using the fermented P. multiflorum using mushroom mycelials.

Lentinula edodes, a member of the Pleurotaceae, is a blood pressure and cholesterol lowering effect. Recently, research the reduction of irritation and the enhancement of function have been cultivation of natural resources using mushroom mycelium. For the development of food fermented with L. edodes mycelium of natural medicinal herb remnants, determined to be 20%, and the product were used after lyophilization. In this study, we were analyzed the contents of β-glucan and amino acids of fermented herb remnants by L. edoeds mycelials. β -Glucan is a type of polysaccharide that has immune-enhancing and is known to inhibit cancer cells. The content of β-glucan and total amino acids of fermented herb remnants by L. edoeds mycelials were 35.13% and 8,697.36 mg%, respectively. The content of essential amino acid from fermented herb remnants by L. edoeds mycelials was 3,226.85 mg%. Collectively, our findings suggest that fermented herb remnants by L. edoeds mycelials extract could be used as an chemo-preventive material for consumers.

β-glucan is a safe and highly potent biological response modifier that nutritionally activates the immune response through the Macrophage, Dendritic and additional immune cells to yield various therapeutic effects. Shiitake mushrooms (Lentinula edodes) contanining β-glucans may be beneficial for human health; they have been used in the treatment of cancer, hypertension, and high cholesterol levels. The effect of different substrates and various developmental stages (mycelium growth, primordium appearance, and fruiting-body formation) on β-glucan production in the edible mushroom L. edodes was studied. The cap of the mature mushroom showed the highest β-glucan activity, and β-glucan activity seemed to be influenced somewhat by some well-known inducers or sawdust. In this study, we utilized five strains (JMI 10022, 10036, 10077, 10079, 10080) of L. edodes regardless of origin and growth conditions. This experiment showed that the expression of β-glucan was induced by glucose bond, and increased with the growing of L. edodes. Quantitatively, reverse transcription PCR utilized pairs of primers specific for β-glucan gene expression shows that expressed genes were most commonly indentified during the process of fruiting-body formation. We suggest that the results will provide valuable information to assist L. edodes industry.

Recently, hair loss regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of 70% ethanol extracts of Phellinus linteus on the growth of fibroblasts, dermal papilla cells and lipid precursors, We want to try to make a materialization. The 70% ethanol extract of P. linteus on the cell viability of Human follicle dermal papilla cell (HFDPC), adipocyte (3T3-L1), fibroblast (CCD-986sk). The result the cell viability of HFDPC was increased according to the contents of ethanol extract of P. linteus. But the 70% ethanol extract of P. linteus showed weak inhibit activity in CCD-986sk cell growth. Also, the 70%　ethanol extract of P. linteus did not inhibit the growth of 3T3-L1 cells. As a result of this study, in-vitro hair growth activity of herbal medicines for hair material development was not shown to be toxic to HFDPC and 3T3-L1 cell. As a result, the 70% ethanol extracts of P. linteus have potential to developing hair-related product. Acknowledgement : This research was supported by the Ministry of Trade, Industry & Energy(MOTIE), Korea Institute for Advancement of Technology(KIAT) through the Encouragement Program for The Industries of Economic Cooperation Region(R0005473)

Honey production from approximately 1.7 million colonies owned by around 21 thousand beekeepers was almost 36 thousand M/T in Korea. Pollen has used as a food and medicine from before the Joseon Dynasty period in Korea. Pollen grains such as acorn (Quercus acutissima), actinidia (Actinidia arguta) and green tea (Camellia sinensis) are popular in the markets in Korea. But stiff pollen wall hindered dissolution of polysaccharides and lowered extraction efficiency. In the present study, we measured the antioxidant activity and the total polyphenol content from the pulverized and lyophilized green tea pollen grains inoculated with 6 kinds of fungi to confirm the husk removal effect. The total polyphenol content of green tea pollens was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in green tea pollen germinated with A. mellea. The total polyphenol content of the lyophilized green tea pollens germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH free radical scavenging method showed that the lyophilized green tea pollen grains germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized green tea pollen grains germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of green tea pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from green tea pollen grains inoculated with A. mellea.

NRPS (non-ribosomal pepetide synthetase) plays an important role in the biosynthesis of secondary metabolites, which includes economically important compounds such as antibiotics, antitumor agents, immune-suppressants, siderophores, toxins, etc. In most cases, however, their functions and biosynthesizing pathways have been still remained riddles because of their cryptic expression and recalcitrant genetics. In our previous genome analyses of Cordyceps bassiana C101, we predicted the sequences of secondary metabolite biosynthesizing gene clusters, which contain non-ribosomal pepetide synthetases whose functions are already known such as beauvericin, bassianolide, bassiatin, tenellin, beauverolides, and oosporein. The authors focused on nrps6 that has not been functionally studied. The single-gene deletion and overexpression mutants were established via Agrobacterium-mediated transformations. PCR, quantitative real-time PCR, and southern blot analyses were carried out to confirm the correct single-gene integrated homologous recombination. In further study, comparative metabolic profiling between wild-type and mutant strains will be followed by their detailed chemical structure analysis through NMR spectroscopy.