Trombiculid mites are known to be the vector of scrub typhus by transmitting rickettsial pathogen, Orientia tsutsugamushi, to human. In this study, we tried to establish a monitoring system for trombiculid mites using chigger mite collecting traps instead of the conventional rodent-capture method. For selection of collecting points, 10 environmental points were chosen from three regions (Taean, Jinan and Chungju) and two field collections were performed in spring (March-May) and autumn (October-November) seasons from 2013 to 2014. Among 10 environmental points, waterway (37.9%), grass field (28.0%), rice field and field near mountain side (11.4%) and reservoir/wet field (7.3%) showed high collecting rates and they should be included for the representative collecting points for surveillance using chigger mite collecting trap. In order to test the possibility that the dried chigger mites from collecting trap can be used for detection of O. tsutsugamushi, we pooled 30, 10 and 5 chigger mites separately and performed the nested PCR. The infection of O. tsutsugamushi was successfully detected from 5 chigger mites pooling sample. This study shows that chigger mite collecting trap could be an alternative method for monitoring system of scrub typhus vectors.

Calepitrimerus vitis (Nalepa) is a grapevine pest and distributed worldwide. This study was conducted to identify grapevine damage related to density of C. vitis and to suggest an effective control time. The damaged flower buds displayed retarded growth and became dried up, and symptoms of damaged leaves such as malformed and bronzed leaves were observed. Flower cluster and leaves were collected and density of the grape rust mite were observed by using a washing and sieving method. The highest mean density (±SE) of C. vitis was found to be 672.6±112.9 per flower cluster with mid-level damage, and 450.1±66.5 per 2 leaves was found on the damaged leaves. These severe damage symptoms were observed in the early spring, and the density of the grape rust mite was significantly higher as 80 times in damaged vineyard than in the undamaged at the early growth stages of the grapevine. To determine the emergence time of the grape rust mite from lower part of a winter bud the ratio of density of C. vitis on upper-bud to lower-bud was observed. On 13 April 2015, 80% of the grape rust mites were observed on upper-bud where the mite could feed at the bud burst stage. Therefore, once the symptoms of damaged flower clusters and leaves are observed, acaricides should be sprayed before inflorescence emergence, and at the early growth stage between bud burst and leaf development in following year. This would allow the decrease of the density of C. vitis in early spring, and the progression of severe damage could be prevented.

본 연구는 공급자(supplier), 중간공급자(distributor) 그리고 고객(customer)으로 구성된 2 단계 공급사슬에서 퇴화성 제품(deteriorating products)에 대한 중간공급자의 재고모형을 분석하였다. 문제 분석을 위해 공급자는 중간공급자의 수요 증대를 목적으로 중간공급자의 주문 크기에 따라 차별적으로 외상 기간을 허용하고, 최종 고객의 수요는 중간공급자의 재고 수준에 따라 선형적(linearly)으로 증가한다는 가정 하에 모형을 분석하였다. 중간공급자의 이익을 최대화하는 경제적 주문량 결정 방법을 제시하였고, 예제를 통하여 그 해법의 타당성을 보였으며, 민감도 분석을 통하여 퇴화율이 재고정책에 미치는 영향을 분석하였다.

Three categories of basic consumer needs are functional, symbolic, or experiential (Shimp, 2010). Functional climbing wear is specially designed garment for outdoor activity and climbing movement using optimized textiles and garment making engineering process. Therefore, visible feature such as color and design could not provide sufficient information of products for proper communication between company and consumer. Product information of functional wear can be delivered to the consumers via Hang-Tag or advertising but the contents of Hang-Tag are too difficult for the consumers to understandfunctional performance of the product (Lee, Bang, & Yoo, 2014). As to advertisements of functional clothing, its contents and appeal types do not much differ from those of fashion apparel providing no functional information with celebrity models; percentage of no functional information ads were the highest (Liu & Yoo, 2014). This study stared to answer the question, ‘is functional information essential in the ads of functional clothing?’ and ‘is it successful?’ The aim of this study, therefore, was to examine the effects of functional information in the advertisements of functional climbing wear. The advertising effectiveness was measured by ‘communicability’, ‘reliability’,‘favorability’ of the advertisement and ‘awareness and reliability of product’, and ‘purchasing related influence’. For the stimulus,10 different types of advertisements were prepared by combining of the following factors: functional information (whether or not to include), appeal type (rational/emotional), and model type (celebrity/non-celebrity/no-human). For the survey, 388 adults in their 20-50’s were participated. The results showed that regardless ofappeal type or model type, functional information enhanced ‘communicability’, ‘reliability’, ‘favorability’ of the advertisement and ‘awareness and reliability of product’, and ‘purchasing related influence’. It implied the importance andnecessity of functional information in the functional climbing wear advertisement.When other variables were controlled, emotional appeal with human models,especially celebrity model, enhanced the advertising effects. Meanwhile, advertisement with rationalappeal and functional information resulted in better ‘favorability’ of ads even without celebritymodels.The finding from this study could be useful information for the functional clothing company to planstrategies for effective advertisement of their products.

Sirtuin proteins are evolutionary conserved Sir2-related NAD+-dependent deacetylases and regulate many of cellular processes such as metabolism, inflammation, transcription, and aging. Sirtuin contains activity of either ADP-ribosyl-transferase or deacetyltranfease and their activity is dependent on the localization in cells. However, the expression pattern of Sirtuins has not been well studied. To examine the expression levels of Sirtuins, RT-PCR was performed using total RNAs from various tissues including liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Sirtuins were highly expressed in most of tissues including the testis. Immunostaining assay showed that Sirt1 and Sirt6 were mainly located in the nucleus of germ cells, spermatocytes, and spermatids in the seminiferous tubules, whereas Sirt2 and Sirt5 were exclusively present in the cytoplasm of germ cells and sperma-tocytes. Our results indicate that Sirtuins may function as regulators of spermatogenesis and their activities might be dependent on their location in the seminiferous tubules.

This studies was conducted to define community structure and diversity of epigeal spiders in apple orchards under different farming practices (organic and conventional). In 11 commercial apple orchards, epigeal spider community structure was investigated during 2012-2014 (3 orchards, 2012; 3 orchards, 2013, 5 orchards, 2014). Epigeal spiders were collected by pitfall traps from April to October, 2012 in Danyang-gun, Chungcheongbuk-do; 2013 in Muju-gun, Jeollabuk-do; 2014 in Cheongsong-gun and Uiseong-gun, Gyeongsangbuk-do, Korea. Sampled spiders were identified to the species level. Biodiversity was analyzed with species richness (total numbers of spider species), abundance (total numbers of individuals) and Shannon-Wiener diversity index (H΄) for each site and compared between organic and conventional orchards. In all regions, abundance of the spider communities were relatively higher in organic orchards than in conventional orchards. But, no significant difference in species richness and specie diversity (H΄) between organic and conventional orchards. Lycosidae was the most dominant family in all orchards regardless farming practices. However, Linyphiidae dominated in organic orchards only. The most dominant species were Pardosa laura (Lycosidae), Erigone koshiensis (Linyphiidae) in organic, Piratula procurvus (Lycosidae) in conventional orchards.

본 연구는 공급자(supplier)가 중간 유통자(wholesaler/retailer)의 수요 증대를 목적으로 중간 유통자의 주문량에 따라 차별적으로 외상 기간을 허용한다는 기본적인 가정 하에 중간 유통자의 재고 모형을 다루었다. 문제 분석을 위하여 최종 고객(customer)의 수요는 중간 유통자의 재고 수준에 따라 선형적(linearly)으로 증가한다는 가정하에 모형을 분석하였고, 모형 분석을 통하여 중간 유통자의 이익을 최대화하는 경제적 주문주기와 경제적 주문량 결정 방법을 제시하고, 예제를 통하여 그 제시된 해법의 타당성을 보였다.

A magnetic powder, M-type barium hexaferrite (BaFe12O19), was consolidated with the spark plasma sin-tering process. Three different holding temperatures, 850℃, 875℃ and 900℃ were applied to the spark plasma sinteringprocess with the same holding times, heating rates and compaction pressure of 30 MPa. The relative density was mea-sured simultaneously with spark plasma sintering and the convergent relative density after cooling was found to be pro-portional to the holding temperature. The full relative density was obtained at 900℃ and the total sintering time wasonly 33.3 min, which was much less than the conventional furnace sintering method. The higher holding temperaturealso led to the higher saturation magnetic moment (σs) and the higher coercivity (Hc) in the vibrating sample magne-tometer measurement. The saturation magnetic moment (σs) and the coercivity (Hc) obtained at 900℃ were 56.3 emu/g and541.5 Oe for each.

Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cul-tured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non-proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn’t stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.

Mushrooms are considered not only as food but also for source of physiologically beneficial medicines. The culinarymedicinal mushrooms may important role in the prevention of age-associated neurological dysfunctions, including Alzheimer’s and Parkinson’s diseases. Hericium erinaceus (H. erinaceus), is edible mushrooms, is a parasitic fungus that grows hanging off of logs and trees and well established candidate for brain and nerve health. H. erinaceus contains high amounts of antioxidants, beta-glucan, polysaccharides and a potent catalyst for brain tissue regeneration and helps to improve memory and cognitive functions. Its fruiting bodies and the fungal mycelia exhibit various pharmacological activities, including the enhancement of the immune system, antitumor, hypoglycemic and anti-aging properties. H. erinaceus stimulates the synthesis of Nerve Growth Factor (NGF) which is the primary protein nutrient responsible for enhancing and repairing neurological disorders. Especially hericenones and erinacines isolated from its fruitin body stimulate NGF, synthesis. This fungus is also utilized to regulate blood levels of glucose, triglycerides and cholesterol. H. erinaceus can be considered as useful therapeutic agents in the management and/or treatment of neurodegeneration diseases. However, this review focuses on in vitro, in vivo and clinical trials for neurodegerative disease.

This study was conducted to investigate the community structure and species distribution of ground beetle assemblages in western part of Yeongwol-gun, Korea. Ground beetles were collected by pitfall traps from 9 study sites during July to October in 2013. A total of 30 species were identified from 4,439 collected ground beetles. Two dominant species, Synuchus nitidus (1,916 individuals) and Synuchus cycloderus (1,900 individuals) were occupied 85.8% of total. Interestingly, Pterostichus orientalis orientalis and Synuchus melantho were only collected in relative high altitude above 350 m, such as Beopheung-ri, Unhak-ri, and Yongseok-ri. Large-sized beetles, such as Damaster, Leptocarabus, and Carabus species, were generally more collected from Suju-myeon (Beopheung-ri and Unhak-ri) and Jucheon-myeon (Docheon-ri and Yongseok-ri) as well-conserved area, but not occurred in Yeongwol-eup and Hanbando-myeon, which forests may have lower habitat quality for these species. These differences also lead to increasing of dissimilarity among study sites, which were diverged into 3 groups at 53.0% of similarity. In particular, species composition of Samok-ri was significantly different at 8.2% of similarity because of difference in habitat characteristics as river side. This study has an important value to provide the fundamental information of inventory and monitoring in Yeongwol-gun. For detail mapping of species distribution in Yeongwol-gun, however, further studies are necessary, especially eastern parts of Yeongwol-gun.

Epigeic spiders (Araneae) were surveyed in apple orchards under different farming practices (organic and conventional) in Muju-gun, Jeollabuk-do, Korea. The study was conducted from April to October in 2013 with 2 weeks interval using pitfall traps. Collected spiders were identified as 20 species of 6 families from 819 individuals in organic orchardⅠ, 19 species of 10 families from 759 individuals in orchardⅡ and 16 species of 8 families from 209 individuals in conventional orchard for from 1,787 captured spiders. Biodiversity was analyzed with species richness, abundance and Shannon diversity index (H΄). Species richness was statistically higher in organic orchard than in conventional orchard. Organic orchardⅡ comprised the greatest abundance followed by organic orchard and conventional. But species diversity was the highest in organic orchardⅡ, intermediate in conventional orchard and the lowest in orchardⅠ. Lycosidae was the most dominant family in all orchards regardless farming practices. However, Linyphiidae dominated in organic orchards only. The most dominant species were Erigone koshiensis (Linyphiidae) in organicⅠ, Pardosa laura (Lycosidae) in organicⅡ and Arctosa pungcheunensis (Lycosidae) in conventional orchard.

This peptide has antibacterial activity against several Gram-positive and Gram-negative bacteria. BmCecB1 is antimicrobial peptides from Bombyx mori and belongs to cecropin family. Antimicrobial peptides are important components of the innate immune systems in all living organism. To produce the BmCecB1 antimicrobial peptide, we constructed transgenic silkworm that expressed BmCecB1 gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 600 eggs of bivoltin silkworms, Baegokjam. In total, 49 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 22 broods, and we selected 2 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 11%. We identified 9 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a BmCecB1 as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that BmCecB1 possesses high antibacterial activities against Gram-negative bacteria.