This study aimed at investigating the gene expression profile in basal ganglia of hexa-valence chromium exposed rat based on cDNA array analysis. For cDNA array, Sprague-Dawley male rats (300 ± 25 g) were administrated with 15 mg/kg B.W/day of potassium dichromate by gavage (0.3 ml) dissolved in saline for 10 days (n=5). For dose-related gene expression analysis, rats were administrated with 0, 1, 5 mg/kg B.W/day of potassium dichromate for 10 days. Control rats were administrated with equal volume of saline (n=5). For cDNA array analysis, RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α³²P]-dATP. Membrane sets of the Atlas array II and Toxicology array kits were hybridized with cDNA probe sets. RT-PCR and Northern blot hybridization methods were employed for validation and assessment of the dose-related gene expression profile, respectively. Among the 2352 cDNAs, 43 genes showed significant (>two-fold) changes in expression. 22 genes were up-regulated and 21 genes were down-regulated in the 15 mg/kg B.W/day hexa-valence chromium treated group than control. According to the Northern blot hybridization analysis, heat shock protein 47, neurodegeneration associated protein 1 and pituitary specific growth factor 1a genes were up-regulated, but Gamma-aminobutyl-acid a1 subunit, neuroligin2, brain calcium-transporting plasma membrane type ATPase genes were down-regulated even in the low-dose of hexa-valence chromium exposed group (1 mg/kg B.W/day) than control. Genes that detected in this study may be closely related to the hexa-valence chromium-induced neurotoxicity in the rat basal ganglia and addition study of these genes can give some more useful information about the neuro-toxic mechanism by hexa-valence chromium.

원자전달 라디칼 중합을 이용하여 poly(styrene sulfonic acid) 47 wt%를 가진 poly(vinylidene fluoride-co-chlorotrifluoroethylene)-g-poly(styrene sulfonic acid) (P(VDF-co-CTFE)-g-PSSA) 가지형 공중합체를 합성하였다. 티타늄 아이소프로폭사이드(TTIP)와 가지형 공중합체를 졸-겔 공정을 통하여 TiO2/가지형 공중합체 복합막을 제조하였다. TTIP는 가지형 공중합체의 친수성을 가진 PSSA 영역에만 선택적으로 결합하였으며 그곳에 TiO2 나노 입자가 형성되어 성장하였다. 이와 같은 결과를 적외선과 자외선 분광학으로 확인할 수 있다. 함수량과 이온 교환 능력 (IEC)는 TTIP의 함량에 따라 감소하였고 이것은 막이 가진 술폰산의 수가 감소하기 때문이었다. TTIP가 5 중량%일 때, 막의 기계적 강도는 증가하고 수소이온 전도도도 유지되었다.

A hierarchical computational method has been developed and used with finite element method based on dislocation density multiple-slip crystalline formulation to predict how nanoindentation affects behavior in face-centered cubic crystalline aggregates. Using displacement profiles which were obtained from molecular dynamics(MD) nanoindentation simulation, scaling relations based on indentation depths, grain-sizes, and grain aggregate distributions were obtained. These relations then applied to coarsen grains in micros- tructurally based FE formulation which accounts for dislocation density evolution, crystalline structures. This computational regime was validated with a several experimental results related to single gold crystals. This hierarchical model provides a tool to link nanosacle level with a microstructurally based FEM formulation that can be to ascertain inelastic effects such as dislocation density evolution. With the above certainty temperature distribution during the nanoindentation simulation also was investigated along with the different indentation depth.

대형 화물자동차의 사고는 일반적인 사고와 달리 심각한 인명피해 등을 동반할 확률이 높다. 장거리 운행이 많은 화물자동차 운전특징으로 인해 운전자의 피로 및 피로로 인한 사고가 높다. 이러한 피로와 관련된 사고를 감소시키기 위해서 외국에서는 운전자의 피로에 영향을 주는 중요변수인 운전시간, 수면시간, 운행형태 등을 고려하여 운전자의 운행시간(Hour-of-Service:HOS)을 법으로 규정하고 있다. 운송업체 또한 운전자들의 사고를 감소시키기 위해 법정 운전자 근무시간 준수를 위해 노력하고 있는 실정이다. 그러나 우리나라는 화물자동차 운전자의 피로와 관련 사고율을 줄일 수 있는 기초적인 법정 운전자 운행시간 조차 제시되지 않고 있다. 본 연구는 우리나라 화물자동차 사고사망자수를 감소시킬 수 있는 운전자의 근로기준법 제정 필요성을 제시하는데 목적이 있다. 이를 위해서 현재 운영 중인 선진외국의 화물자동차 운전자 운전시간 및 운행규정을 살펴보고 실증적 자료를 통해 운전시간에 따른 사고 위험도의 차이를 살펴보았다. 실증적 자료는 미국의 화물자동차 3곳의 사고 자료 231명의 운행일지와 사고가 나지 않은 자료 462명의 운행일지를 수집, 총 693명 운전자의 운행일지를 수집하였다. 운전자의 연속된 운행에 대한 특징을 반영할 수 있도록 time-dependent 로지스틱 회귀모형을 사용하였다. 분석결ㄹ과 운행시간 1시간부터 3시간까지 운전한 운전자 사고위험도 차이는 없는 것으로 나타났다. 운행시간이 10시간인 운전자는 운행시간 1시간인 운전자보다 사고위험도가 약 2.2배 높은 것으로 분석되었다. 결론적으로 운행시간이 증가할수록 사고위험도 또한 증가한다는 것을 알 수 있다. 또한 본 연구는 우리나라의 지역적 특성과 운전자 특성에 맞는 운전자 운전시간 설정을 위한 연구방향 및 향후 연구과제에 대하여 연급하였다.

The term servant-leadership was first coined in a 1970 essay by Robert K. Green Leaf (1904-1990) entitled “The Servant as Leader”. In the development of leadership theories, there has been no consensus for the definition of leadership. In the course of explosion of many theories in the middle of 20th century, many people were tired of the emphasis on the styles, traits and other secrets for successful leadership based on the achievement. In this context, the servant leadership opens a way to give room for human beings, that is, relationship and emphasizes the importance of leaders’ identity rather than methodology. Greenleaf defines Servant leader as what is sharply different from the person who is leader first. The difference manifests itself in the care taken by the servant-first to make sure that other people's highest priority needs are being served. But because the words servant and leader are usually thought of as being opposites, they frustrate and annoy scholars and practitioners. Furthermore, some critics criticized Greenleaf’s writings are discursive because he never defines nor argues for any coherence in the features of leadership that he proposes. Others also point out that its biblical background is not based on the sound Christology. To overcome the shortcoming of the servant leadership theory, witness-based leadership, which is based on the atonement and the great commission of Christ, is suggested in this paper. The servant role is not inferior to the vocation of witness, but the two are seen as constituting a whole.

This study attempts to develop e-learning contents for the “fashion information analysis,” which should be performed in combination of theory and practice in fashion education, in a recent Web-based education environment. The e-learning contents are developed by using the three steps of analysis, design and development. In order to analyze the requirements of curricula, the status and content of fashion information related curricular provided by general universities and cyber universities are analyzed, and then on the basis of this analysis the education objectives and detailed contents of e-learning contents are determined. Design and development of e-learning contents consist of the types, education menus and education form. The types of e-learning contents are developed into an education form combined with tutorial and practice/activity types. The education menus are comprised of syllabic, the profiles of professors and students, announcements, questions and answers regarding respect subjects, materials, and notes. Meanwhile, the education form consists of the following sections: “Before the Learning”, “Learning”, “Review Questions”, “Final Review”, “References”, and “Practice”, “Before the Learning” consists of two parts of a theory lecture that is composed of video clips and Power-Point presentations, and practice that consists of an audio(or video) lecture and illustration or Photoshop software execution window.

This research was intended to furnish basic data which can be helpful to maintain and improve the skin health and to activate aromatherapy by analyzing the knowledge, attitude and behavior on the aromatherapy skin care of college students. As their grade got higher, students of beauty class showed the level of knowledge on aromatherapy higher than those of non-beauty class, and that of girl students was higher than that of boy students and as the living standard of students got higher, and pocket money of a month of them got more, the level of knowledge on aromatherapy of them was high, and as the interest in appearance and satisfaction on appearance of them got higher, the level of knowledge on aromatherapy of them was high. In common characteristics, as the living standard of them got higher, and pocket money of a month of them got more, the level of attitude on aromatherapy of them was high. As the interest in appearance of them got higher, the level of attitude on aromatherapy on the interest in appearance of them was high. In the characteristics related with the skin, as the skin health condition of students got healthier, the level of attitude on aromatherapy of them was high, and it was high in the students who had much agony on the skin color.