이 연구는 약용버섯 번데기 동충하초로부터 특이적 자실체 형성 유전자를 선별하기 위하여 수행하였다. cDNA는 버섯의 분화 4단계 균사체, 원기, 미성숙 자실체, 성숙한 자실체로부터 분리한 각각의 total RNA를 이용하여 합성하였다. 특이적으로 발현된 유전자의 선별은 cDNA와 랜덤한 primer set을 이용한 DD-PCR에 의해 수행되었고, pGEM 클로닝 벡터를 이용하여 6개의 partial 유전자 서열을 확인하였다. 6개의 DD-PCR product는 보고된 유전자와 유사도를 확인하기 위해 NCBI BLAST search를 사용하여 GenBank에서 비교하였고, 6개의 유전자는 보고되지 않은 유전자임을 확인하였다.

This study is to investigate the trend of researches on food and culture from 1990 to 2003. With a literary approach, this paper analyzes how many papers were published and what was the major research subject. We classified the journals published during this time into 10 different categories and we are to grasp a research trend. 1) Historical approach on the traditional food and food habit; 2) Globalization of Koran food; 3) Use and preparation for traditional food; 4) Table setting and table manner; 5) Provincial cuisine; 6) Traditional festive food or celebratory meal; 7) Religious food; 8) Fusion food; 9) Food and culture in foreign countries and food habit of Korean people living abroad; 10) Studies related with satins out. It was revealed that 268 papers(=ps) were published. Eating out was the most frequently reported subject(100 ps), followed by provincial cuisine(54 ps), use and preparation for traditional food(49 ps), food habit for foreign people and Korean people living abroad(22 ps). Researches on these 4 topics have been accelerated since the late half of 1990s. This reflects the social factors such as rapid growth of food service industry, settlement of local autonomy, popularization of leisure activities and accelerated introduction of foreign food and culture. Half of the studies on the eating out dealt with eating out behavior. And 30 papers were reports on the food service industry situations. More than half of the studies on the provincial cuisine(26 ps) were concentrated on digging out recipes. Studies on the use and preparation for traditional food were mainly about what kind of food and how often the food is used(17 ps). Kimchi was the most frequently studied food. There were 11 papers regarding food and culture in foreign countries. Most of the authors were historians or linguists. Food habit of Korean people living abroad were investigated in 7 papers. There was few studies on fusion food, table setting and table manner. This doesn't meet with increasing demand for specialized information in then fields. Researches on the traditional festive food or celebratory meal(5 ps) and religious food(9 ps) were negligible. Papers on the historical approach to the traditional food and food habit were limited(12 ps). Moreover, most of them were patchwork of existing literatures. Continuous researches to exploit the historical facts based on literary proof should be tried with patience. Otherwise, the papers will copy the hackneyed knowledge repeatedly. Globalization of Korean food means the development of Korean food for foreign people and export of them worldwide. Only 16 papers were reported on this subject, 14 of them were published after 2000, 8 of them were surveys on the foreigners' food preference. In order to get practical informations on what we develope and how we sell for the foreign customers, profound research on their food habit should be done.

IEC 60300 series, the international standards on reliability management system are newly developed and introduced rapidly worldwide. In order to meet this fast-changing situation, it is necessary to apply them to the Korean industries effectively. It includes the establishment of the infrastructure such as documentation of Korean Standards, introduction of test equipments and test manuals, accreditation of the certification bodies and training of the specialists etc. IEC 60300 is upgraded management system of the ISO 9000 and ISO 14000. In this regard, the Korean companies which were certified with the ISO 9000 or 14000 should endeavor to find new strategies and methods to be certified with IEC 60300. This paper introduces a Quantitative method of estimating the level of the reliability management system by applying the MIR(Maturity Index on Reliability) technique. This enables the estimation of the operation level of the system and can upgrade the maturity level of it.

IEC 60300 series, the international standards on reliability management system are newly developed and introduced rapidly worldwide. In order to meet this fast-changing situation, it is necessary to apply them to the Korean industries effectively. It includes the establishment of the infrastructure such as documentation of Korean Standards, introduction of test equipments and test manuals, accreditation of the certification body and training of the specialists etc. IEC 60300 is upgraded management system of the ISO 9000 and ISO 14000. In this regard, the Korean companies which was certified with the ISO 9000 or 14000 should endeavor to find new strategies and methods to be certified with ISO 60300. This paper introduces a quantitative method of estimating the level of the reliability management system by applying the MIR(Maturity Index on Reliability) technique. This enables the estimation of the operation level of the system and can upgrade the maturity level of it.

이 연구는 1991년부터 2003년까지 발간된 〈교정연구〉에 실린 논문에 대한 내용분석을 통해서 교정학의 연구영역과 연구동향에 관한 논의를 하고 있다. 총 207편의 논문을 분석의 대상으로 하여 연구주제와 연구방법론적 특성과 경향을 밝혀내고 있다. 그 주요 결과를 보면 먼저, 연구주제는 행형관련 주제에 국한되지 않고 상당히 다양했으나, 주제별로 상대적 중요도는 달랐다. 교정행정과 수용자관리, 그리고 교정처우의 법률적, 제도적인 논의는 풍부한 반면 교정의 다른 축인 수용자의 일상생활이나 독특한 문화를 이해하는 논의, 그리고 교정 프로그램의 실질적 효과를 측정하는 효과분석 논문은 양적으로 상당히 미미한 수준이었다. 한편 연구주제에 접근하는 방식인 방법론에 있어서는 대부분이 기술(description)수준의 문헌연구였으며, 실증연구인 경우에도 추론적 통계(inferential statistics)보다는 기술통계(descriptive statistics)를 제시하는 수준에 머물러 있었다. 이러한 결과를 바탕으로 보다 교정학의 발전을 위해 더 관심이 주어져야 할 주제와 방법론적 향상을 위한 제언을 제시하고 있다.

Bacillus thuringiensis는 포자형성기 동안에 위생해충이나 농업해충에 독성을 보이는 내독소 단백질을 생성한다. 내독소 단백질의 모기 유충 방제효과를 높이기 위해, 광합성에 관여하는 psbA promoter로 모기 살충성 cry11Aa유전자를 발현하는 pSyn4D벡터를 제작하고, 모기 유충이 먹이로 이용하는 Synechocystis PCC6803에 형질 전환시켰다. 형질 전환체들은 kanamycin이 포함된 배지에서 선발되었으며, 정상적인 생물검정을 통해 형질 전환체 Tr2C를 선발하였다. cry11Aa 유전자는 형질전환체의 genomic DNA에 안정적으로 결합되어 있는 것을 PCR을 이용하여 확인하였다. 형질전환체 Tr2C는 약 72-kDa크기의 Cry11Aa 단백질을 발현하였으며, 작은빨간집모기(Culex tritaeniorhynchus) 3령 유충과 중국얼룩날개모기(Anopheles sinensis) 3령 유충에 75%가 넘는 살충력을 보였다. 모기 유충에 대한 형질전환체의 반수치사시간(LT)은 작은빨간집모기 유충과 중국얼룩날개모기 유충에 대해 각각 2.1일과 0.7일이었다. 이상의 결과들은 형질전환체 Tr2C가 모기 유충방제에 유용하게 이용될 수 있음을 보여준다.

Most radio astronomy issues at WRC-03 (World Radiocommunication Conference-03) revolved around satellite downlink allocations, particularly to NGSO (Non-Geostationary Satellite Orbit) satellite systems, in bands adjacent to or close to a radio astronomy frequency band. Out of a total of 50 agenda items, ten were of interest to radio astronomers. This paper provides some details about the important outcome of the radio astronomy related issues at the WRC-03.

The first sentence in the second paragraph of INTRODUCTION, 'The first discovery of a galaxy beyond z=5 was reported by Weymann et al. (1998); HDF 4-470.3 at z=5.60.' should be read as 'The first discovery of a galaxy beyond z=5 was reported by Dey et al. (1998); 0140+326 RD1 at z=5.34'. The authors sincerely regret this error.