The Ansan Reclaimed Wetland, reclaimed in 1986 and maintained for 38 years, underwent two wetland function assessments to develop strategies for enhancing biodiversity and conservation. The biota of the reclaimed wetland showed an increase in the number of species observed across most taxa, while the number of amphibian and reptile species decreased. Among legally protected species and specific species, Aix galericulata and Lefua costata were not observed, whereas Libellula angelina were introduced. The results were categorized into “protection or improvement” and “enhancement.” Proposed conservation measures include removing planted shrubs, maintaining waterways, and installing gabion structures and wildlife corridors.

This study was conducted to confirm whether domestic fruit tree pruning residues can replace sawdust, which is the main ingredient of mushroom medium. The five types of fruit tree pruning residues collected were from apples, pears, peaches, grapes, and citrus. The basic components of these residues were analyzed. The pH ranged from 5.2 to 7.0, the Total carbon(T-C) ranged from 46.0% to 47.2%, the Total nitrogen(T-N) ranged from 0.5% to 0.9%, and the moisture content ranged from 12.4% to 14.2%, which was identified as an appropriate range for growing mushrooms. In order to confirm the possibility of mycelial growth of oyster mushroom "Suhan", column tests were conducted based on the conventional medium(poplar sawdust 49.5: cotton seed hull 27.3: beet pulp 12.7: cotton seed meal 10.5, v/v). As a result of incubation at 25°C for 28 days, grape branches showed the fastest growth at 143 mm compared to the control, which showed 135 mm. The yield per bottle was comparable, with grape branches(134g/bottle) and control(139g/bottle). Additionally, the quality of the fruiting bodies was comparable across all Processed lots. It means grape branches can be used as alternative sawdust materal. In the future, it is expected that using by-products as substitutes for carbon sources and main ingredients will help reduce farm production costs and protect the environment.

The winter forage triticale (x Triticosecale Wittmack) cultivar ‘Onggoljin’ was developed in 2018 by Central Area Crop Breeding Division, the National Institute of Crop Science, Rural Development Administration, as a winter forage. ‘Onggoljin’ has green leaves with medium length and narrower blade width compared to ‘Joseong’. ‘Onggoljin’ has shorter length spikes, a slightly longer awn than ‘Joseong’, and it’s anther without anthocyanin color. The seeds of ‘Onggoljin’ was shorter in both length and thickness than those of ‘Joseong’, with a yellowish-brown grain coat color. The heading date of ‘Onggoljin’ was at April 23 nationwide on average, two days later than ‘Joseong’. It exhibits stronger winter hardness, and simmilar lodging and desease resistance compared to ‘Joseong’. The plant height of ‘Onggoljin’ was longer than that of ‘Joseong’, and its fresh matter yield and dry matter yield were 46.31 tons/ha and 15.75 tons/ha, respectively, representing 18% and 17% higher than ‘Joseong’. The forage quality characteristics of ‘Onggoljin’ at late milky stage were crude protein content of 6.2%, neutral detergent fiber content of 59.5%, acid detergent fiber content of 34.4%, and total digestible nutrients content of 61.8%, and the silage quality was grade 2. The grain yield of ‘Onggoljin’ was 6.57 tons per ha.

Background: The ability of adeno-associated viruses (AAVs) to transduce various cell types with minimal immune responses renders them prominent vectors for gene editing (GE), with different AAV serotypes exhibiting distinct transduction efficiencies due to their specific cellular tropism. However, detailed molecular processes of AAV infection and penetration, as well as the optimal serotype for specific purposes, remain poorly understood. Porcine models are widely used in research benefitting both human and livestock due to anatomical and physiological similarities to humans. Methods: Transduction efficiencies of 18 AAV serotypes (AAV1–9, 6.2, rh10, DJ, DJ/8, PHP.eB, PHP.S, 2-retro, 2-QuadYF, and 2.7m8) were evaluated in immortalized porcine lung epithelial cells (pLCsImt) and pulmonary alveolar macrophages 3D4/31 (PAMs 3D4/31). Results: We found AAV2, DJ, and 2.7m8 to be the most effective in both cell types. The highest enhanced green fluorescent protein expression of 52.46 ± 2.4% in pLCsImt and 64.08 ± 2.4% in PAMs 3D4/31 was observed for AAV2, while negligible transduction was observed for AAV4, rh10, DJ, PHP.eB, PHP.S, and 2-retro. AAV-DJ showed superior transduction efficiency in PK-15, as compared to AAV2 and 2.7m8. Results emphasize the cell type-specific nature of AAV serotype transduction efficiencies. Notably, AAV2 was most effective in both lung and macrophage cells, whereas AAV-DJ was more effective in renal cells. Conclusions: Our findings suggest that AAV2 was identified as the most efficient serotype for transducing pLCsImt and PAMs 3D4/31, compare to the PK-15 cells. Understanding cell type-specific preferences of AAV serotypes offer crucial insight for tailoring AAV vectors to specific tissue and optimizing genome editing strategies, with potential implications for the advancement of personalized medicine and development of treatments for human and livestock.

Clove (Syzygium aromaticum) is a highly valued medicinal plant native to Aisa. Widely used as a spice, renowned for its medicinal properties, particularly in Ayurveda and traditional Chinese medicine. In this study, clove bud extract (CBE) was prepared at different ethanol concentrations of 50%, 80%, and 90%, respectively. The antioxidant activity of the CBE was evaluated through DPPH, polyphenol, and reducing power assays, revealing its strong antioxidant potential, with 90% ethanol being the most effective extract. HPLC analysis identified eugenol (8.7 mg/g) as the major active compound, known to possess anti-inflammatory and antioxidant properties. Given the role of oxidative stress and inflammation in atopic dermatitis (AD), the therapeutic potential of CBE was explored using a 1-chloro-2, 4-dinitrobenzene (DNCB)-induced AD mouse model. Five-week-old BALB/c mice were induced with AD by topical application of DNCB. CBE was administered topically to the affected skin (back and ear) areas for 4 weeks. The treatment of CBE significantly reduced the severity of clinical dermatitis, decreased epidermal thickness, and lowered mast cell and eosinophil infiltration in skin tissue, as observed through hematoxylin eosin staining and toluidine blue staining. The results demonstrated CBE as a promising therapeutic agent for managing AD through its regulation of skin inflammation and oxidative stress, making it a potential candidate for future treatments of inflammatory skin disorders.

Tricholoma matsutake, one of the most famous edible mushroom in Asia, has been cultivated in red pine forest. Because of its difficulty in artificial cultivation, T. matsutake cultivation has relied on foraging in the forest. Under certain environmental conditions, T. matsutake form the Shiro, the condensed mycelium, and develop into fruiting bodies. Among the certain environmental conditions, fungal communities play a major role in the mushroom development. Therefore, fungal community investigations for Bonghwa and Yangyang have been conducted on soil with fairy rings in the past, soil with existing fairy rings, and soil with presumptive fairy rings developing in the future. From the six soil samples, total 163 genera of fungi were detected and species diversity and species abundance of each sample were also analyzed. In result, the species diversity and species abundance of the fairy rings in Yangyang were lower than those in Bonghwa. In comparison with the fairy rings in Yangyang and Bonghwa, the dominance of the genus Tricholoma was higher and that of the genus Motilella was lower in Yangyang. Through the continuous study, establishment of optimal environmental conditions to promote the T. matsutake cultivation is highly expected.

This study examined the spatiotemporal changes in Jilnal Wetland using satellite image-based time series data and field surveys. The analysis focused on changes in open water surface area and vegetation using the JRC Surface Water Monthly Water History dataset and Landsat imagery from 1984 to 2020. The impact of hydrological changes on vegetation was assessed through surveys conducted before and after the 2023 monsoon season. Results indicated a continuous decrease in open water surface area since the 1980s, with a sharp decline post-2003. NDVI analysis showed an inverse relationship between water area reduction and increased vegetation vigor and coverage. Field surveys revealed that hydrological changes directly affected vegetation structure, leading to new herbaceous communities and greater diversity post-monsoon. These findings highlight significant ecosystem changes driven by anthropogenic factors and natural succession, necessitating a comprehensive conservation strategy, including development control, hydrological management, terrestrialization prevention, and long-term monitoring.

목적 : 본 연구에서는 노래의 템포(bpm) 및 데시벨(dB)에 따른 동공 크기 및 동적 조절 변화를 비교하고, 빛의 유무에 따른 차이도 비교해 보고자 한다. 방법 : 연구 대상은 안질환이나 시각 장애가 없는 성인 20명(남자 10명, 여자 10명, 평균연령 24.25±3.99세) 이었다. 완전 교정 상태에서 명소시에서 6 m 거리의 시표로, 암소시에는 무한대 거리로 측정하였다. 두 조건 모두 헤드셋을 착용하여 네 가지 음악 드럼(D), 메탈(M), 외국 팝(F), 클래식(C)을 들었을 때 동공 크기와 조절 반응의 변화를 각각 40 dB 미만, 60 dB 미만, 80 dB 이상으로 측정하여 비교하였다. 결과 : bpm 및 dB에 따른 자극이 있을 때, 유의한 차이를 보였다(p<0.010). 두 조건 모두 동일한 dB에서 bpm 이 달라졌을 때 자극량의 차이는 있었으나, bpm 변화에 따른 동공 크기와 동적 조절 변화량은 유의한 차이가 없었 다. 동공 크기와 동적 조절 변화량을 조도에 따라 비교했을 때, D 장르의 40 dB(p<0.050)과 F 장르의 60 dB (p<0.050)에서 동공 크기 변화량의 차이가 있었다. 조도가 없을 때 동적 조절 변화량이 더 증가하여, bpm과 dB에 따른 모든 그룹에서 유의한 차이가 나타났다(p<0.050). 결론 : 본 연구 결과, 각 노래의 bpm과 dB에 따른 동공 크기와 동적 조절 변화는 자극의 유무에 따라 영향을 받는 것으로 확인되었다. 특히, 암소시에서는 동적 조절 변화에 더 큰 영향을 미쳤으며, 명소시에서는 동공크기 변 화가 컸다.

Background: This study was conducted to increase meat production by 30% compared to the present by selecting a giant cow over 1,000 kg and applying biotechnologies. Methods: After OPU from 1,100 kg of giant cow, the calves were produced in Hanwoo surrogate mothers. Among 23 calves six male heads were selected, three heads of them were chosen as candidate sires, and the rest three heads were raised for 30 months for performance test. The semen of three candidate sires from the age of 22 months were collected and frozen, and the calves were produced by artificial insemination. The calf was raised to the age of 30 to 33 months and performed a progeny test. Results: The average birth weight of 23 calves born by transferring giant cow-derived embryos was 42.8 kg, and the average weight of carcass from three bulls was 615.3 kg in the performance test. In the progeny test, the average birth weight of calves born after artificial insemination of semen from giant cow-derived candidate sires was 41 kg, and the average weight of carcass after raised to the age of 30 to 33 months was 562.7 kg. As a result of performance and progeny tests, it is 148 and 96 kg higher in giant cow-derived beef cattle than the average carcass (467 kg) from general Hanwoo cattle, respectively. Conclusions: This study will have laid a great foundation for the future improvement of the Korean beef industry.

This study was conducted to investigate changes in the productivity of Italian ryegrass seeds according to the timing of harvest after heading in the southern region. The Italian ryegrass variety ‘Greencall’ was sown in Jinju, Gyeongsangnam-do, in the fall of 2022. Four harvest timings were tested (30, 40, 50, and 60 days after heading), with a randomized complete block design and three replicates. Sowing in the test plots took place on October 10, 2022, and harvesting was carried out from 30 days after heading on May 18 to 60 days. The plant height was the longest (99.1 cm) in the plot harvested 30 days after heading and decreased as the harvest was delayed. No significant differences were observed among treatments in terms of lodging resistance, disease resistance, and cold resistance. However, lodging severity increased over time after heading (7∼9). Disease incidence was also higher in plots harvested 50 and 60 days after heading. The length of the spike was shortest (38.76 cm) in the plot harvested 50 days after heading, and the number of seeds per spike was the lowest (42 seeds/spike) in the plot harvested 60 days after heading. The dry matter content of seeds increased with the delay in harvest, while dry matter yield decreased, with the lowest yield observed in the plot harvested 40 days after heading (3,031 kg/ha, p<0.05). The dry matter content of seed straw was highest at 75.73% in the plot harvested 50 days after heading and dropped to 34.99% 60 days after heading due to rainfall. The dry matter productivity of the seed straw was the lowest in the plot harvested 40 days after heading. The feed value of the seed straw also decreased with delayed harvest, with an average RFV (Relative Feed Value) of 91. In conclusion, the optimal harvest timing for fall-sown Italian ryegrass intended for seed production in the southern region appears to be 30 days after heading.

Tricholoma matsutake is an economically important ectomycorrhizal mushroom, but artificial cultivation remains challenging. While much research has been conducted on the bacteria and fungi associated with T. matsutake, studies on archaea have been limited. This study compared and analyzed the diversity and structural differences of archaeal communities in the fairy ring and non-fairy ring soils using a metabarcoding approach. The alpha diversity analysis revealed that the archaeal community in fairy ring soil had lower diversity compared to non-fairy ring soil, and beta diversity analysis clearly separated the community structures between the two soil types. Among all analyzed soils, Nitrososphaeria (Nitrososphaerota) dominated the archaeal community at class level. In fairy ring soils, Nitrosotalea (Nitrosotaleaceae) was predominant, while in non-fairy ring soils, Nitrosocaldaceae dominated. These results suggest that Nitrosotalea may have a close association with T. matsutake, highlighting the need for further in-depth research on archaea as potential growth promoting microorganism for T. matsutake mycelial growth.

This study investigated the relationship between changes in language learning beliefs and English proficiency among 41 Korean university students who participated in a short-term English program. Participants’ beliefs were assessed using the Beliefs About Language Learning Inventory (BALLI), and their proficiency was measured using the Test of English for International Communication (TOEIC). Frequency analysis, descriptive statistics, paired-sample t-tests, and correlation analysis were employed to analyze the data. The study found significant improvements in both listening and reading scores, and changes in beliefs varied with proficiency gains. Students with higher proficiency gains demonstrated improved confidence and self-efficacy, and decreased instrumental motivation, whereas those with lower gains exhibited minimal changes in beliefs. Correlation analysis revealed that belief shifts, such as reduced selfconsciousness and increased integrative motivation, were positively related to proficiency gains. These findings suggest the dynamic nature of learners’ beliefs and their potential impact on language learning outcomes, highlighting the importance of addressing belief systems in English language education.

목적 : 이 연구는 COVID-19 팬데믹 전후로 중년층에서 근거리 작업 시간이 증가함에 따라 근시 발달에 미치는 영향을 분석하는 데 중점을 두고 있다. 또한, 팬데믹 기간 동안 생활 방식의 변화가 근시 악화에 어떻게 기여했는지도 평가한다. 방법 : 질병관리청 국민건강영양조사 제8기 자료를 기반으로, 2019년과 2021년 동안 수집된 중년층(40~60세) 의 굴절력 데이터를 분석하였다. 성별, 연령, 근거리 작업 시간 등을 포함한 설문조사 데이터를 바탕으로 통계분석 을 수행하였으며, T-test와 카이제곱 검정(χ²)을 통해 두 시기 간 굴절력 변화를 비교하였다. 결과 : 2021년 팬데믹 이후 중년층의 근시가 유의미하게 악화되었으며, 특히 3시간 이상의 근거리 작업 시간이 고도 근시로의 진행을 가속화시키는 것으로 나타났다. 오른쪽 눈의 굴절력 악화가 더 두드러졌으며, 이는 작업 습 관 및 안구 피로와 관련이 있을 수 있다. 결론 : COVID-19 팬데믹 동안 근거리 작업 시간의 급증이 중년층의 근시 진행에 중요한 영향을 미쳤음을 확인 하였다. 향후 중년층의 시력 보호를 위해 실외 활동 증대와 디지털 기기 사용 시간을 줄이는 예방적 조치가 필요하다.

For centuries, humans have leveraged the health-promoting properties of plants for our well-being. While research has been conducted on numerous medicinal plants, the specific benefits of many species remain underexplored. Eupatorium Japonicum (EJ), a member of the Asteraceae family, has historically been consumed in Japan, South Korea, China, and Vietnam for its traditional use in soothing digestive issues. This study aimed to explore the radical scavenging and antiinflammatory efficacy of EJ extract using RAW 264.7 cells. The radical-scavenging effects were assessed using the DPPH and ABTS assays, where an anti-oxidative molecule in the test sample will react with a stable free radical in DPPH and ABTS causing discoloration. The anti-inflammatory efficacy was assessed using the nitric oxide (NO) assay in LPS-induced RAW 264.7 cells, where the amount of NO produced in response to infection was measured using Griess reagent. Reversetranscriptase polymerase chain reaction (RT-PCR) and real-time PCR were executed to confirm the anti-inflammatory activity by measuring the RNA levels of pro-inflammatory cytokines. The DPPH and ABTS assays revealed that EJ extract decreased oxidation in a concentration-dependent manner (7.8-1,000 μg/mL) compared to ascorbic acid and Trolox respectively. EJ extract significantly reduced NO production concentration independently. Furthermore, EJ extract showed no cytotoxic effects as determined through the MTT assay. RT-PCR and real-time PCR analyses revealed inhibition of mRNA expression of pro-inflammatory cytokines (iNOS, COX-2, TNF-α, and IL-6). Western blotting demonstrated EJ’s anti-inflammatory activity by reducing protein levels of iNOS, COX-2, TNF-α, and IL-6. These findings suggest that EJ extract exhibits anti-inflammatory activities and can be further evaluated in the future.

Alfalfa hay varieties have been developed and cultivated in Korea to enhance the self-sufficiency rate of domestic roughage. The objective of this study was to compare the effects of feeding domestic and imported alfalfa on the growth performance and economic feasibility of Hanwoo bulls. This study was conducted using eight Hanwoo bulls, aged 6 to 7 months, during their growing period. Hanwoo bulls were divided into two groups: one group fed imported alfalfa and the other group fed domestic alfalfa. Dry matter intake, weight gain, and blood metabolites were analyzed for two months of the growing stage. There was no significant difference between the two groups in dry matter intake and body weight. However, the price of domestic alfalfa is lower than that of imported alfalfa, so feeding domestic alfalfa is expected to have a positive effect on reducing production costs. Further study is needed to compare not only the growth performance but also carcass characteristics after slaughter by feeding domestic alfalfa to Hanwoo for a longer period during their growing stage.

Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts. Methods: In vitro fertilized (IVF) eggs were cultured in IVC medium (control) with the addition of mTeSR1 supplement at concentrations of 1%, 2%, and 5%, respectively. The development rates of fertilized eggs and gene expression patterns of blastocysts were assessed on day 9 of culture. For outgrowth culture, blastocysts were cultured on a mouse embryonic fibroblast feeder cells (MEFs) for 7 days. Results: In vitro development of bovine preimplantation embryos in the 2% mTeSR1 group was significantly higher than in the control (p < 0.05). The apoptotic index in the 2% mTeSR1 group was significantly lower compared to the control (p < 0.05). RTqPCR indicated that SRY-Box Transcription Factor 2 (Sox2) gene expression in the 5% mTeSR1 group was significantly higher than in the control (p < 0.05). The 5% mTeSR1 group also showed significantly higher BCL2 associated X (Bax) expression compared to the control and other mTeSR1 groups. On day 9 pi, blastocysts from the control and 2% mTeSR1 groups were cultured for 7 days. The 2% mTeSR1 group showed higher efficiency in forming dome-shaped colonies with stronger SOX2 expression compared to the control. Conclusions: The mTeSR1 supplement supports preimplantation embryo development and prevents apoptosis in blastocysts, leading to the efficient formation of domeshaped inner cell mass (ICM) colonies.

Climate change significantly impacts biodiversity, particularly for endemic species in restricted habitats. This study focuses on the Korean fir (Abies koreana), an alpine conifer species in South Korea, to evaluate potential habitat changes under SSP climate scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5- 8.5). Using high-resolution ensemble climate data from the KMA (Korea Meteorological Administration) and integrating 10 species distribution models (SDMs) into an ensemble model, we predicted habitat suitability for the period 2010~2090. The species data was refined and constructed with a focus on location data so that it could be used in this model, using the National Ecosystem Survey, Baekdudaegan Conservation Area Survey of the National Institute of Ecology, and the National Park Natural Resources DB of National Park Service. The results identified BIO1 (mean annual temperature) and BIO13 (Precipitation of Wettest Month) as the most influential bioclimatic variables for habitat suitability. SSP1-2.6 exhibited fluctuating habitat area with partial recovery by 2070s, while SSP5-8.5 projected a near-complete loss of suitable habitats by 2090s. The ensemble model demonstrated robust performance, providing reliable predictions across all scenarios. This study highlights the substantial impact of climate change on the habitat suitability of Abies koreana and underscores the importance of understanding these changes to preserve vulnerable alpine ecosystems.

이 논문은 1920년대 초반 아일랜드의 독립전쟁과 이후 독립을 둘러싼 정치적 갈등에서 비롯된 아일랜드의 내전을 배경으로 종교와 중산층의 기득권을 유지 하려는 자유국을 지지하는 테디와 공화국의 완전한 통일과 독립을 주장하며 노동자의 이상적인 세상을 꿈꾸는 다미엔이 결국은 서로를 파멸시키는 갈등과 비극을 사회주의 적 관점에서 분석한다. ≪보리밭을 흔드는 바람≫에서 로치는 역사적 사실의 교묘하고 도 의도적인 왜곡을 통해 관객들이 실체를 파악할 수 있도록 영화를 제작했으며 역사 적 사실보다는 진정한 의미를 이해하는 것이 더 중요하다고 주장한다.

Afoxolaner is an insecticide and acaricide that belongs to the isoxazoline chemical compound group. it has been used as an active pharmaceutical ingrdient in veterinary medicine to treat fleas and ticks in dogs. When patents expire between 2026 and 2066, it is expected that many products will be applied for approval as generic products, and reserch to establish accurate quality control methods must be conducted and managed. HPLC method was developed for the quantitative and qualitative of afoxolaner in veterinary medicinal products. The separation of active constituents for afoxolaner was achieved on a RP18 (4.6 x 150 mm, 5 μm) column using Water : Acetonitrile : MeOH (25:30:45 v/v/v) as mobile phase, with UV detection at 245 nm. The method was validated for specificity, linearity, accuracy and precision. All calibration curves showed good linearity (R2 of 0.999) within the concentration ranges (12.5 to 400 μg/mL). For accuracy, the recovery rate was calculated by spiking three concentrations of standard into the sample blank. The recovery rate was calculated to be 99.70~100.58%. Precision was measured 9 times repeatedly through intra-day, inter-day tests using standard. It showed excellent precision by satisfying the relative standard deviation of less than 2% both intra-day and inter-day. Limit of detection (LOD) and limit of quantitation (LOQ) were 2.0 μg/mL and 6.1 μg/mL, respectively. This method was successfully applied to analyzing afoxolaner drugs distributed in Korea. The HPLC method described in this study is accurate and reproducible and could be applied for the analysis of veterinary drugs of afoxolaner.

This study examined domestic consumption trends in the development of mushroom-based alternative food products. It found that 85.5% of consumers were aware of alternative foods, with a higher recognition rate among younger age groups and variations based on household composition and monthly income. Additionally, 70% of consumers familiar with alternative foods had tried them. Among those who had not, taste (16%) and purchasing challenges (15%) were the primary barriers. Minced meat was the most commonly consumed alternative (25%), while dumplings (7%) and burgers (1%) were less popular. Notably, dumplings and burgers showed the highest potential for development using mushroom-based ingredients. These findings provide valuable foundational data for advancing mushroom-based alternative food products.