Background: Single nucleotide polymorphisms (SNPs) are widely used genetic markers with applications in human disease diagnostics, animal breeding, and evolutionary studies, but existing genotyping methods can be labor-intensive and costly. The aim of this study is to develop a simple and rapid method for identification of a single nucleotide change. Methods: A modified Polymerase Chain Reaction Amplification of Multiple Specific Alleles (PAMSA) and high resolution melt (HRM) analysis was performed to discriminate a bovine polymorphism in the NCAPG gene (rs109570900, 1326T > G). Results: The inclusion of tails in the primers enabled allele discrimination based on PCR product lengths, detected through agarose gel electrophoresis, successfully determining various genotypes, albeit with some time and labor intensity due to the use of relatively costly high-resolution agarose gels. Additionally, high-resolution melt (HRM) analysis with tailed primers effectively distinguished the GG genotype from the TT genotype in bovine muscle cell lines, offering a reliable way to distinguish SNP polymorphisms without the need for time-consuming AS-PCR. Conclusions: Our experiments demonstrated the importance of incorporating unique mismatched bases in the allele-specific primers to prevent cross-amplification by fragmented primers. This efficient and cost-effective method, as presented here, enables genotyping laboratories to analyze SNPs using standard real-time PCR.

Currently, the number of animals administered in animal hospitals and drugs prescriptions has increased. At the same time, the number of cases of violation of administrative measures due to violations of the Narcotics Control Act has also increased. Therefore, it is necessary to review whether the current status of drug prescriptions, management and supervision systems in animal hospitals are correct and whether there is anything to be supplemented. Although drug handling is managed through NIMS, there are problems such as poor supervision due to the NIMS single system, reporting exceptions that form blind spots for management supervision, and lack of connection between prescriptions and medical departments within the system. In order to overcome these problems, the following improvement plans are proposed. First, reduces the hassle of handling reports and facilitates verification of medical records and prescriptions by strengthening the link between NIMS and animal hospital electronic charts, and strengthens the link between drug handling reports of medical institutions and drug handling reports of animal hospitals to prevent exposure to medical shopping. Second, by introducing a preliminary notification system into the veterinary system, drug misuse is prevented through prior notification in accordance with the criteria for preventing misuse of animal drugs. Third, by modifying the NIMS reporting system by introducing disease classification symbols, the exact cause of the increase in use is identified by preparing drug use data for each disease. Based on these improvements, it is expected that a management system that reflects the actual medical environment of animal hospitals will be established and drug abuse will be prevented based on guidelines.

Pigs are considered a “mixing vessel” that can produce new influenza strains through genetic reassortments, which threaten public health and cause economic losses worldwide. We performed surveillance of swine influenza virus (SIV) using 114,140 nasal swabs from 11,414 Korean farms from 2009 to 2022, and characterized their genetic evolution at each farm level. A total of 169 farms out of the 11,414 (1.48%) were SIV-positive. The positivity rate for the H1N2 subtype, which is most dominant in the pig population, was 37.87% (64/169). Through hemagglutinin (HA) gene analysis, 64 H1N2-positive farms were classified into Eurasian avian-like (46/64, 71.88%), triple-reassortant (14/64, 21.88%), pdm09 (3/64, 4.69%), and classical swine (1/64, 1.56%) groups. The estimated evolutionary rate of HA in H1N2 from 2009 to 2022 in Korea is 1.5309 × 10-3/site/year (95% HPD intervals from 1.0003 × 10-3 to 2.1735 × 10-3) with an estimated mean growth rate of 0.0114. Estimates of the relative genetic diversity of clades over time suggested that the HA of H1N2 exhibited an increase in population size. The results of this study showed that the Eurasian avian-like-HA of the H1N2 subtype was dominant in the pig population. The continued evolution of the HA of H1N2, which is critical for cell entry, might lead to genetic diversity and the loss of vaccine cross-protection. These results indicate that continus surveillance is imperative for monitoring the evolution of the swine influenza virus.

This study investigates the social construction and continual (re)production of the cultural Other through educational materials, focusing on Korean middle school English textbooks. Twelve middle school English textbooks from four publishers were collected and analyzed, with a focus on how they (mis)represent cultures and practices of minority groups. Utilizing critical content analysis to examine both visual and written texts in the textbooks, this research uncovers recurring patterns of exoticizing and commodifying Hawai’ian culture, homogenizing African culture, and romanticizing Indigenous peoples. The findings contribute significantly to our understanding of how cultural representations in English as a Foreign Language (EFL) textbooks shape perceptions and influence societal dynamics. They also illuminate how these representations reflect and perpetuate Eurocentric colonialist discourse. Moreover, the results underscore the importance of equipping educators, especially English teachers, with critical literacy. This empowers them to identify hidden power relations that reinforce stereotypes, and actively engage in the construction of a more inclusive and equitable society.

The optimization of a prevention program utilizing a novel trivalent inactivated Salmonella bacterial vaccine to protect poultry from Salmonella infections was evaluated in this study. A total of 50 brown nick layers were divided into 5 groups, A to E, each containing 10 hens. Group B hens were immunized with the SG9R vaccine, group C chickens were immunized with the trivalent inactivated Salmonella bacterial vaccine, and group D hens were primed with SG9R and boosted with the trivalent inactivated Salmonella bacterial vaccine. Group E hens were injected with sterile PBS. All hens in groups B to E were orally challenged with a mixture of wild-type S. Enteritidis, S. Typhimurium, and S. Gallinarum (approximately 6×10⁹ CFU/ 0.2 ml/bird). Serum IgG titers, CD3+CD4+ T-cell levels, and CD3+CD8+ T-cell levels in group D were significantly higher than those in group A. Additionally, all animals in groups A to D showed no clinical symptoms and survived after the virulent challenges, whereas all chickens in group E died following the challenge. The challenge strains of S. Enteritidis, S. Typhimurium, and S. Gallinarum were not isolated from the liver, spleen, cecum, and cloaca of group D chickens. These findings indicate that priming with SG9R and boosting with a trivalent inactivated Salmonella bacterial vaccine can be an effective approach for preventing Salmonella infections by inducing robust protective humoral and cellular immune responses in chickens.

Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

This study evaluated the fungicidal efficacy of weakly acidic hypochlorous acid water (WAHW) against Microsporum canis (M. canis) and its therapeutic effect on M. canis-infected mouse skin. WAHW was produced by a WAHW generation module. A fungicidal efficacy test by the broth dilution method was used to determine the lowest effective concentration of the WAHW. The lowest effective concentration of WAHW was less than 10 ppm. For T-1, T-2, and T-3, 30 ppm of WAHW was applied to the infected skin once, twice, and three times a day, respectively, and for T-4, 50 ppm of WAHW was applied once a day. On the 3rd day after the initiation of treatment, skin scores in all of the WAHW-treated groups were significantly decreased compared to those in the positive control group (PC) (p<0.05), and there were no significant differences compared to the normal control (NC). The area of the infected skin in all of the WAHW-treated groups was significantly decreased compared to PC from the first day after the initiation of WAHW treatment (p<0.05). The results showed that WAHW had a fungicidal efficacy on M. canis at less than 10 ppm, and it was effective in improving skin symptoms when 30 ppm of WAHW was applied to the M. canis-infected area once a day for five days or 50 ppm of WAHW was applied once a day.

Canine hyperadrenocorticism, a prevalent endocrine disorder characterized by excessive cortisol production. Notably, hypercoagulability leading to pulmonary thromboembolism (PTE) poses a substantial concern. PTE may be underestimated because of the rapid dissolution of canine thrombi postmortem. However, traditional coagulation assays face challenges in early detection of hypercoagulability. Therefore, this study explored the use of thromboelastography (TEG) as a diagnostic tool for identifying hypercoagulability in dogs with hyperadrenocorticism. A total of 31 dogs visited the Gyeongsang Animal Medical Center between 2018 and 2022, comprising 21 dogs with hyperadrenocorticism and 10 controls who underwent clinical and coagulation analyses. Hyperadrenocorticism was diagnosed using a low-dose dexamethasone stimulation test or adrenocorticotropin hormone stimulation test, and conventional laboratory parameters and coagulation parameters, such as the prothrombin time, activated partial thromboplastic time, fibrinogen, and TEG results, were compared between the groups. Clinical data revealed significantly elevated monocyte, platelet, alanine aminotransferase, alkaline phosphatase, triglyceride, and cholesterol concentrations in dogs with hyperadrenocorticism, which were attributed to excess cortisol secretion (p<0.05). TEG analysis demonstrated significantly decreased K values and increased α and MA values in hyperadrenocorticism dogs (p<0.05), indicating a shortened clotting time and enhanced clot strength, suggestive of hypercoagulability. TEG effectively highlights hypercoagulability in dogs with hyperadrenocorticism and provides valuable insights in predicting blood clot formation. Although predicting clot formation in dogs remains complex owing to multifactorial influences, this study underscores the potential utility of TEG in enhancing such predictions for dogs with hyperadrenocorticism.

PURPOSES : This study aims to conduct a laboratory evaluation on the use of ferronickel slag for manufacturing Hot Mix Asphalat mixtures. METHODS : This research was based on laboratory evaluation only, where conventional aggregate and FNS at a ratio of 7:3 were used in HMA and the volumetric properties, physical and mechanical properties, and long-term performance of FNS in asphalt mixture were evaluated. RESULTS : The overall results showed that FNS can be applied as aggregate in a hot mix asphalt since volumetric, physical and mechanical properties and long-term performance of HMA mixture with ferronickel slags as aggregate met the required standards according to Korean standards for Asphalt Concrete. CONCLUSIONS : The tensile strength ratio results of HMA mixtures with ferronickel aggregate did not meet the required standards, yet the addition of anti-stripping agent and waste glass fibers to the HMA mixture with ferronickel slags improved the tensile strength results to meet the standards. Additionally, compared to the HMA mixture of the same aggregate gradation but with only natural aggregate, HMA mixture with ferronickel slags had almost the same results for the majority of tests conducted.

이 연구는 생성형 AI(Generative AI) 기술을 활용하여 기본 시각디자인 분야에서의 새로운 접근 방법과 가능 성을 탐색한다. Generative AI는 데이터 기반 학습을 통해 창의적인 디자인을 생성하는 인공지능 기술로, 시 각디자인에서 중요한 역할을 하고 있다. 본 연구는 Generative AI가 시각디자인의 기본 요소와 원리에 어떻게 적용되며, 디자인 프로세스를 어떻게 혁신할 수 있는지를 분석한다. 먼저, Generative AI가 색채, 형태, 구성과 같은 디자인의 기본 요소를 어떻게 해석하고 재창조하는지에 대해 연구한다. 이를 통해 AI가 디자인의 창의 성과 예술성을 어떻게 향상시킬 수 있는지를 탐구한다. 또한, AI가 디자인 결정 과정에서 어떻게 인간 디자 이너를 보조할 수 있는지에 대해서도 연구한다. 이 연구는 Generative AI를 실험하고, 이를 통해 얻은 시각디 자인 결과를 통해, AI가 시각디자인의 전통적인 접근 방식에 어떤 새로운 시각과 해석을 제공하는지를 조사 한다. AI 기술의 발전이 디자인의 미래와 디자이너의 역할에 어떤 변화를 가져올지에 대한 통찰과 디자인 분 야의 전문가뿐만 아니라, AI 기술에 관심 있는 학자들에게도 중요한 시사점을 제공한다는 점에서 의의를 찾 을 수 있다.

In this study, quantitative analysis is attempted on data collected from Chilgapsan Observatory Star Park in Cheongyang-gun, Chungcheongnam-do. The aim of this experimental study in which quantitative analysis of the Astronomical Science Museum in Korea is conducted is to investigate its current situation and secure basic data. As of July 31, 2023, it has had 283,931 cumulative visitors in total. It had the largest number of visitors when it opened (2009 year), after which their number reduced steadily until the pandemic (COVID-19, 2020–2022). Recently, however, the number of visitors has increased. Generally, the number of visitors is highest in August (20.8$\%$) and least in April (4.1$\%$). The visit rate is higher on weekends (Saturday and Sunday) than on weekdays (Monday–Friday), and groups comprise only about 5.3$\%$ of the total number of visitors. Moreover, it can be confirmed that the number of visitors increases sharply during events. Finally, it was confirmed that the visit rate was unaffected by weather conditions. Considering these results, we propose the following strategies: 1) Establish a special program that reflects “the weekend effect.” 2) Prepare a plan to attract group visitors during the weekdays using “the event effect.” 3) Arrange alternative programs (e.g., experiential activities) that can be conducted indoors regardless of weather conditions. We think that our findings will help establish a roadmap for the direction the Astronomical Science Museum should take and aid in preparing a strategic foundation to preemptively respond to unexpected situations (e.g., pandemics).

가상의 공간에서 함께 즐기는 콘텐츠에 대해 관심이 늘어나고 있다. 더불어 가상공간에서 나만의 아바타도 꾸미고 게임 콘텐츠 역시 즐기고 있다. 이러한 가상의 공간을 메타버스라고 하며 대표적인 플랫폼으로는 제 페토와 로블록스가 있다. 메타버스 플랫폼은 플랫폼의 취지에 맞게 유저가 직접 제작하기 편리한 컨텐츠 제 작 도구를 지원한다. 그러나 일반 유저가 제작한 콘텐츠는 메타버스에서 활동하고 있는 다양한 유저의 관심 과 흥미를 지속적으로 유발하기에는 부족한 부분이 있어 꾸준한 흥행이 어렵다. 본 논문은 기존 게임 엔진을 활용해서 콘텐츠를 제작하는 콘텐츠 개발자로서 메타버스 플랫폼이 제공하는 제작 도구의 효용성을 검증하 고자 게임 엔진의 선택 기준을 참고하여 인터페이스, 개발 언어, 개발 공정의 기준으로 대표하는 플랫폼의 제작 도구를 비교하였다.

This study investigated primary teachers’ knowledge of pronunciation instruction, and its manifestation in classroom practices in Korean EFL contexts. To this end, the questionnaire data collected from 47 teachers were quantitatively analyzed. The emerging themes from 5 teachers’ interviews were qualitatively analyzed, based on content analysis. Findings revealed that they had an appropriate knowledge base of pronunciation teaching, equipped with the better understanding of content knowledge (CK), followed by pedagogical content knowledge (PCK), and technical pedagogical content knowledge (TPACK). No statistically significant difference was found in teachers’ knowledge between males and females, and also between the 4 groups with different teaching experience. They manifested their knowledge mainly acquired from the past course lectures into classroom practices, employing controlled and free practices such as listening and repeating, shadowing, songs, chants, games, and role play, including different types of corrective feedback. Most notably, they integrated role play effectively into classroom practices to improve young learners’ pronunciation in interestprovoking and enjoyable ways.

In recent years, deaths and missing people have continuously occurred due to man overboard (MOB) and suicide on passenger ships. However, due to the complex deck structure and enlargement of passenger ships, closed-circuit television (CCTV) coverage is limited, and it is difficult to prevent accidents for all passengers with limited crews. Therefore, a real-time system for detecting high-risk blind spots on passenger ships is needed through risk analysis. This study used a combination of the following three risk factors to calculate and evaluate the risk of MOB and suicide by deck area of the passenger ship: 1) distance away from guard rails, 2) the visibility of CCTV, and 3) ship operating conditions. Based on the survey from experts, risk scores of MOB and suicide accidents by deck area on a passenger ship were yielded.

본 연구에서는 디지털 게임 내 성폭력 피해 경험과 가해 경험의 내용과 특성에 대해 탐색하고자 하였다. 성폭 력 피해/가해 경험의 구체적인 내용과 정도, 성폭력 경험으로 인한 정서적 영향, 성폭력 피해시의 대처양식 등 을 조사할 수 있는 질문지를 개발하여 20세 이상의 게임 사용자들을 대상으로 온라인 설문을 실시하였고, 총 267명의 자료가 분석되었다. 그 결과, 연구참여자의 99.3%가 1회 이상 성폭력 피해를 경험하였고, 성폭력 가해 도 연구참여자의 62.5%가 경험한 것으로 나타났다. 또한 성폭력 피해와 가해 모두에서 간접적 성희롱이 가장 높은 수준으로 경험되고 있었다. 피해 경험으로 인한 정서적 영향에 있어, 지속적인 성폭력 피해가 있는 경우, 피해자들은 분노/짜증, 우울/불안, 적대감/스트레스를 유의미하게 높게 경험하였다. 피해 경험 시 대처전략은 ‘게임 내 신고시스템에 신고’가 가장 높게 나타났고, 다음으로 부정과 회피가 뒤따랐다. 가해 경험으로 인한 정서적 영향의 경우, 가해 상위 10% 집단이 하위집단에 비해 가해 행동을 통해 스릴/흥분과 같은 심리적 자극 을 경험하는 동시에 죄책감 등의 부정적 정서도 높게 경험하는 것으로 나타났다. 성폭력 피해/가해 경험의 정 도를 기준으로 집단 간 차이를 비교한 결과, 게임 이용 행동에 있어 집단 간 차이가 있는 것으로 확인되었다. 본 연구는 특정 게임이 아닌 다양한 장르와 특성의 게임들에서 발생하는 성폭력의 내용과 정도 등의 구체적 인 실태를 파악했다는데 의의가 있다. 또한 해외 연구에서 사용된 연구 틀을 그대로 적용하지 않고, 우리나라 게임 사용자들의 디지털 게임 내 성폭력 경험에 대해 실제적으로 접근하였다는데 의의가 있다.

In this review, the regulatory mechanisms of autophagy were described, and its interaction with apoptosis was identified. The role of autophagy in embryogenesis, tooth development, and cell differentiation were also investigated. Autophagy is regulated by various autophagy-related genes and those related to stress response. Highly active autophagy occurrences have been reported during cell differentiation before implantation after fertilization. Autophagy is involved in energy generation and supplies nutrients during early birth, essential to compensate for their deficient supply from the placenta. The contribution of autophagy during tooth development, such as the shape of the crown and root formation, ivory, and homeostasis in cells, was also observed. Genes control autophagy, and studying the role of autophagy in cell differentiation and development was useful for understanding human aging, illness, and health. In the future, the role of specific mechanisms in the development and differentiation of autophagy may increase the understanding of the pathological mechanisms of disease and development processes and is expected to reduce the treatment of various diseases by modulating the autophagic phenomenon.

This study investigated the effects of multisensory memory strategies of pairing visual and aural learning strategies of aural lexical advance organizers (LAO) and read-alouds on 146 Korean high school students learning the meaning and pronunciation of 18 unfamiliar English words. In this quasi-experimental design, the control group learned the words on a single mode of written LAO and silent reading as opposed to two treatment groups of aural LAO and silent reading, and of aural LAO and read-alouds, respectively. The effects were tested three times via pre-, post-(immediately after learning), and delayed (30 days later) tests. The immediate and long-term effects were examined by detecting the differences across the three groups in post- and delayed-tests by one-way ANOVA, and the retention of effects was examined by paired t-tests in each group across the three tests. The results indicated that pairing aural LAO and read-aloud strategies was most effective in learning and retention of both vocabulary meaning and pronunciation.

본 연구에서는 자기공명영상 검사 시 와인색 반영구 문신용 염료가 신호 강도에 미치는 영향을 분석하고, 전자파흡수 에 의한 발열 감소 방안을 연구하고자 하였다. 한천과 와인색 반영구 문신용 염료를 이용하여 염료가 퍼진 형태의 팬텀(패드형 팬텀)과 한곳에 뭉친 형태의 팬텀(주사형 팬텀)을 제작하였다. 두 팬텀은 전신용 자기공명영상장치에서 고속스핀에코(turbo spin echo, TSE)와 경사에코(gradient echo, GRE) 계열의 펄스열을 이용하여 2D와 3D로 검사하였다. 검사 후 영상 분석은 팬텀의 몸체, 염료, 공기 방울, 배경을 대상으로 총 720회의 신호강도를 측정하였 다. 전자파흡수에 의한 발열 감소 방안을 확인하기 위해 삽입형 온도계를 팬텀에 삽입 후 검사 전후 팬텀의 온도 변화를 확인하였다. 이때, 대기 온도의 상승 변화를 고려하기 위해 검사 전 대기 온도를 측정하였고, 발열 감소를 위한 방법으로 장치에 내장된 팬, 젖은 거즈, 얼음을 이용하였다. 신호 강도는 2D 영상보다 3D 영상이 좋았고, TSE 계열이 GRE 계열보다 좋았다. 특히 와인색 반영구 문신용 염료가 있는 부위의 신호강도는 공기 때문에 발생한 자화 율 인공물보다 신호강도가 낮게 측정되었다. 온도 측정 검사는 패드형 팬텀과 주사형 팬텀 모두 검사에 의한 온도상 승 효과가 있었으며 온도의 저감을 위한 방법으로 얼음 패드, 팬, 젖은 거즈 순서였다. 본 연구를 통해 와인색 반영구 문신용 염료는 자기공명영상 검사에서 영상과 환자의 안전에 문제를 일으킬 수 있음을 확인하였고, 발열 감소 방안으 로 얼음 패드가 효과적이었다는 것을 확인할 수 있었다.

형식은 텍스트와 컨텍스트 사이의 상호작용으로 발생하여, 이중 어느 하나로도 귀결되지 않는 동시에 이들 사이를 조정한다. 형식은 단순히 텍스트의 부산물이 아니고 비가의 예에서처럼 사회적 관습에 종속된다. 동시에 형식은 컨텍스트와 별개로 독립적인 형식의 패턴을 유지한다. 알리와 멀둔에 관한 이 비교연구는 형식이 가진 이러한 역동성을 전제로 진행된다. 두 시인은 역사적 구체성과 구조적 자율성에 기반한 전통적인 시 형식이 가진 역동성을 사용하여 고국이 식민주의 이후 극심한 분쟁을 겪을 때 노스탤지아적 세계관을 구축한다. 두 시인은 스스로를 망명자로 인식하며 가잘, 소네트, 세스티나, 빌라넬과 같은 시 형식을 통해 노스탤지아적 갈망을 표현한다.