The sweetpotato whitefly Bemisia tabaci is one of the most serious pests in the greenhouse. They can infest variouscrop plants even in high warm greenhouse condition in summer. To understand the effect of heat acclimation ability ofB. tabaci, mortality was determined at various temperature conditions of the greenhouse. Adult whiteflies survived evenin 63°C in tomato greenhouse. The median lethal temperature (LT50) of the greenhouse colony was 59.9°C however LT50of the lab colony was 47.6°C. The relative expression level of heat shock proteins (hsp23, hsp70 and hsp90) were significantlylower in the greenhouse colony compare to the lab colony. This result suggests B. tabaci has very high thermal adaptability(acclimation) and its mechanism is associated with heat shock protein system.

To identify genes that commonly respond to the treatment of different insecticides and are responsible for the toleranceenhancement, transcriptomic profiles of larvae treated with sublethal doses of the five insecticides were compared withthat of untreated control. A total of 117,181 transcripts with a mean length of 662 bp were generated by de novo assembly,of which 35,329 transcripts were annotated. Among them, 125, 143, 182, 215 and 149 transcripts were determined tobe up-regulated whereas 67, 45, 60, 60 and 38 genes were down-regulated following treatments with these five insecticides.The most notable examples of commonly responding over-transcribed genes were two cytochrome P450 genes and ninecuticular protein genes. In contrast, several genes composing the mitochondrial energy generation system were significantlydown-regulated in all treated larvae. Considering the distinct structure and mode of action of the five insecticides tested,the differentially expressed genes identified in this study appear to be involved in general chemical defense at the initialstage of intoxication. Their possible roles in the tolerance/resistance development were discussed.

West Nile Virus (WNV) is transmitted by infected mosquitoes. Vector mosquitoes usually acquire these pathogens fromfeeding on an infected host, and transmit the pathogens to a naive host during feeding events. To understand the virustransmission dynamics and to survey WNV throughout country, the present study has been conducted. We collected mosquitoesat urban parks in Seongnam, Wonju, Gunsan, Daegu, and Tongyeong using CDC light trap with Dry ice from April toSeptemper in 2017 (mosquito collecting is on going). Among collected mosquitoes, blood-fed mosquitoes were conductedblood meal identification assay and the other mosquitoes were subjected to virus detection using real-time PCR method.A total of 2,290 mosquitoes representing 6 genera and 15 species were collected. The most dominant species was Culexpipiens complex (42.1%) followed by Aedes albopictus (15.1%), Ae. vexans nipponii (14.6%), Ochlerotatus koreicus (9.8%),Cx. orientalis (6.5%), and Armigeres subalbatus (4.4%). The blood meal source were of mammal (93.3%), and birds (6.7%).So far, no WNV has been detected in any mosquitoes.

The members of the genus Flavivirus are noteworthy, as they cause infectious diseases in humans, such as Zika, denguefever, yellow fever, West Nile, and Japanese encephalitis. Due to the increased awareness of the public health risk posedby flavivirus-infected mosquitoes, mosquito collections were performed in six urban parks of South Korea, as the parksare designated for human recreation but also provide suitable habitats for mosquitoes. We examined the diversity andabundance of mosquito species and conducted molecular diagnostics for the detection of flavivirus infections. Monthlycollections were carried out in each park from March to August in 2017. A total of 4,851 mosquitoes (5 genera and13 species) were collected using BG-sentinel traps and then investigated for flavivirus infections. Pathogenic flavivirusinfections causing human diseases were not observed in the field-collected mosquitoes. However, insect-specific flavivirus(ISF) infections were detected in several mosquito pools. ISF has been previously known to enhance or suppress the replicationof medically important flaviviruses in co-infected mosquito cells. In this study, partial sequences of ISF were analyzed.However, further studies are needed in order to determine its genetic characterization and biological function in vivo.

Oyster mushrooms are popular and physiologically beneficial to human health. This study was conducted to investigate the effect of dietary supplementation of oyster mushrooms such as Pleurotus ostreatus, P. pulmonarius, and P. citrinopileatus on high cholesterol fed rats. Five-week old female Sprague-Dawley albino rats were divided into three groups of 8 rats each. Feeding a diet containing a 5% fruiting body powder of Pleurotus species to high cholesterol fed rats reduced plasma total cholesterol (TG), triglyceride (TG), low-density lipoprotein (LDL). Feeding each oyster mushroom also significantly reduced body weight in high cholesterol fed rats. However, it had no adverse effects on plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, total protein or enzyme profiles such as glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and alkaline phosphatase (ALP) which are related to kidney function. Feeding each mushroom increased total lipid and cholesterol excretion in feces. A histological study of hepatic cells by conventional red O staining showed normal findings in mushroom-fed hyperlipidemic rats. These results suggested that diet supplementation of 5% fruiting body power of Pleurotus species provided health benefits by acting on the atherogenic lipid profile in high cholesterol fed rats.

The Protocol for China’s accession into the WTO stipulated certain differential treatment for China, including the determination of normal value in anti-dumping investigations for the transitional period of 15 years. This treatment was authorized by the Protocol in response to concerns raised by other WTO Members at the time of China’s entry into the WTO. Since the transitional period is over in November 2016, there is an argument supporting the grant of market economy status to China, although the Protocol does not require the automatic grant of market economy after the passage of the transitional period. However, China’s recent trade measures, which have been adopted to press another WTO Member to meet its political objective, raise a question as to whether China is indeed ready for market economy status. This article analyzes such case and offers a view on the grant of market economy status to China.

This study was conducted on the “traditional area” of Dunsan in Daejeon, and 22 apartment complexes in Doan new city in the “new urban area”. Its goal was to compare and analyze green percentage of each apartment complex’s exterior space. The application examples of the green percentage were examined. Through the advanced research, exterior space types of apartment housing were divided according to chronical change. Five kinds of space (entry space, main pedestrian space, auxiliary pedestrian space, building surroundings space, and playground space) were designated to enable comparison analyses between green-space percentages of the apartment complex in both the Dunsan and Doan areas. Five exterior spaces and 22 apartment complexes in the Dunsan area and in the Doan new urban area were photographed. This study serves as a basic exploratory study to derive the baseline Index of Greenness results of traditional area and new urban area, addressing the lack of such research on the residential facilities. The study also analyzed differences between the two greenness indexes with respect to shape and space. Compared with the average rates of index of greening in traditional areas and new urban areas, Doan (a new urban area) showed an Index of Greenness rate of 34.1%, and Dunsan (a traditional area) showed an Index of Greenness rate of 40.5%, which was 6.4% higher. It is considered that the shape of each green space, the arrangement of various facilities, and the design of the spaces have a great influence on the differences of the Index of Greenness.

In this work, the effects of hydrogen reduction on the microstructure and thermoelectric properties of (GeTe)0.85(AgSbTe2)0.15 (TAGS-85) were studied by a combination of gas atomization and spark plasma sintering. The crystal structure and microstructure of TAGS-85 were characterized by X-ray diffraction(XRD) and scanning electron microscopy (SEM). The oxygen content of both powders and bulk samples were found to decrease with increasing reduction temperature. The grain size gradually increased with increasing reduction temperature due to adhesion of fine grains in a temperature range of 350 to 450 °C. The electrical resistivity was found to increase with reduction temperature due to a decrease in carrier concentration. The Seebeck coefficient decreased with increasing reduction temperature and was in good agreement with the carrier concentration and carrier mobility. The maximum power factor, 3.3 × 10−3 W/mK2, was measured for the non-reduction bulk TAGS-85 at 450 °C.

Selecting an appropriate antigen with optimal immunogenicity and physicochemical properties is a pivotal factor to develop a protein based subunit vaccine. Despite rapid progress in modern molecular cloning and recombinant protein technology, there remains a huge challenge for purifying and using protein antigens rich in hydrophobic domains, such as membrane associated proteins. To overcome current limitations using hydrophobic proteins as vaccine antigens, we adopted in silico analyses which included bioinformatic prediction and sequence-based protein 3D structure modeling, to develop a novel periodontitis subunit vaccine against the outer membrane protein FomA of Fusobacterium nucleatum. To generate an optimal antigen candidate, we predicted hydrophilicity and B cell epitope parameter by querying to web-based databases, and designed a truncated FomA (tFomA) candidate with better solubility and preserved B cell epitopes. The truncated recombinant protein was engineered to expose epitopes on the surface through simulating amino acid sequence-based 3D folding in aqueous environment. The recombinant tFomA was further expressed and purified, and its immunological properties were evaluated. In the mice intranasal vaccination study, tFomA significantly induced antigen-specific IgG and sIgA responses in both systemic and oral-mucosal compartments, respectively. Our results testify that intelligent in silico designing of antigens provide amenable vaccine epitopes from hard-to-manufacture hydrophobic domain rich microbial antigens.

This study examines the construction and management of physical facilities in natural recreational forests that have been created after gaining an understanding of how visitors will use them. It also studies operations tailored for the use of visitors to natural recreation forests. The study presents basic data pertaining to management of natural recreation forests, analyzes the impact of current facilities on visitor satisfaction, and reveals the potential for new programs to be implemented. The independent variables in this study are facilities and program establishment/management and program utilization pertaining to the operation of natural recreation forests. The considered variables include facilities management and facilities requirements categorized by state programs. Program needs were analyzed using correlation. The state of facilities management in natural recreation forests, utilization program implementation, and demand for utilization programs were found to have a positive (+) influence on visitor satisfaction. The results of the rank regression analysis of the influence of the independent variables on visitor satisfaction decreases in the following order: state of facilities management, utilization program implementation, and demand for utilization programs. Visitors to natural recreation forests prefer recreational activities that provide a break from their normal routine, rather than the use of other aggressive utilization programs. Therefore, a higher priority should be given to ensuring appropriate provision of facilities that generate higher visitor satisfaction, as well as their stable management, compared to other utilization programs. Therefore, there are some indications that natural recreation forests of the future will benefit from the development and implementation of an essential set of utilization programs that will have a positive (+) impact on visitor satisfaction.

Modern men are much more interested in their appearance than ever before, as well as showing an increased need for uniqueness in order to construct their own sphere and pursue differentiation from others. Besides, changes in life style and the aesthetic sense are causing men to pursue various kinds of clothing benefits so that they want a suit with characteristics beyond the stereotyped design and style. They have started to be actively engaged in clothing purchases, forming a driving force for the growth of the male suit market. Hence, this study has significance in that it offers data conducive to consumers’ custom suit purchase by reviewing data on the present condition of the custom suit market and bespoke suits. In addition, it aims to give a summary on the theoretical bases of preceding studies, including the need for uniqueness, pursuit of clothing benefit and concern for the appearance of male consumers, as well as examining the factors that influence on their bespoke suit purchase intentions. Accordingly, this work intends to construct a basic environment for consumers to approach the custom suit market easily and pave the way for male suit markets through offering marketing data and information that is helpful for custom suit-related markets.

Beneficial effects of glucosinolate in Chinese cabbage on human health have propelled researches on biochemical and genetic characteristics of glucosinolate and Chinese cabbage. However, growth conditions which are practically important in producing functional Chinese cabbage and in optimizing glucosinolate have been little focused. The objective of this study, hence, was to identify growth conditions affecting glucosinolate contents so that the result could be used for further optimizing glucosinolate in Chinese cabbage. We used principal component analysis (PCA) for analyzing glucosinolate contents in Chinese cabbage cultivated under various growth conditions in the plant factory. As a result, PCA showed that two principal components were able to explain more than 76% of variations in glucosinolate contents caused by different growth conditions. The first principal component (PC1) was mainly represented by humidity and temperature, while growth duration was the main component of PC2. From these results, it was conclusive that glucosinolate contents in Chinese cabbage were largely affected by humidity, temperature and growth duration.

We investigate two abnormal CME-Storm pairs that occurred on 2014 September 10 - 12 and 2015 March 15 - 17, respectively. The first one was a moderate geomagnetic storm (Dstmin  -75 nT) driven by the X1.6 high speed flare-associated CME (1267 km s−1) in AR 12158 (N14E02) near solar disk center. The other was a very intense geomagnetic storm (Dstmin  -223 nT) caused by a CME with moderate speed (719 km s−1) and associated with a filament eruption accompanied by a weak flare (C9.1) in AR 12297 (S17W38). Both CMEs have large direction parameters facing the Earth and southward magnetic field orientation in their solar source region. In this study, we inspect the structure of Interplanetary Flux Ropes (IFRs) at the Earth estimated by using the torus fitting technique assuming self-similar expansion. As results, we find that the moderate storm on 2014 September 12 was caused by small-scale southward magnetic fields in the sheath region ahead of the IFR. The Earth traversed the portion of the IFR where only the northward fields are observed. Meanwhile, in case of the 2015 March 17 storm, our IFR analysis revealed that the Earth passed the very portion where only the southward magnetic fields are observed throughout the passage. The resultant southward magnetic field with long- duration is the main cause of the intense storm. We suggest that 3D magnetic field geometry of an IFR at the IFR-Earth encounter is important and the strength of a geomagnetic storm is strongly affected by the relative location of the Earth with respect to the IFR structure.