“Hyemi”, a new covered cultivar derived from the crosses between “Suwon 298” and “Milyang 34/Albori//Dongbori” developed at the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. The origin of “Hyemi” is “Suwon 400” (SB951032-B-B-B-28). The initial cross was made in 1995 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 400” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hyemi”. The characteristics of “Hyemi” were the following: rate Ⅳ growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 29 in upland and April 23 in paddy field, which was similar and 2 days earlier than that with check cultivar, “Olbori”. The culm length was 81 cm which was 4 cm shorter than those with check cultivar. It showed spike length of 4.1 cm and 646 spikes per m2, 52 grains per spike, 33.4 g of 1,000-grain weight and 672 g of test weight. It showed similar winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It showed higher protein content, whiteness and total phenol content than those of the check cultivar, but lower β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 3.95 ton ha-1 in upland and 4.38 ton ha-1 in paddy field, which was 13% and 16% higher than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.

Field experiments were conducted to determine the physiological and biochemical basis of the interactive effect of sulphur (S) and nitrogen (N) application on seed and xanthotoxin yield of Ammi majus L. Six treatments were tested (T1 = control-without manure and fertilizers, T2 = manure @ 9 kg plot-1-10~;t~;ha-1,~;T3=A0N50K25P25,~;T4=S40N50K25P25,~;T5=S40N100K25P25~;T6=S20+20N50+50K25P25) ). Nitrate reductase (NR) activity and ATP-sulphurylase activity in the leaves were measured at various phonological stages, as the two enzymes catalyze rate-limiting steps of the assimilatory pathways of nitrate and sulphate, respectively. The activities of these two enzymes were strongly correlated with seed and xanthotoxin yield. The highest nitrate reductase activity, ATP-sulphurylase activity and xanthotoxin yield were achieved with the treatment T4 . Any variation from this treatment decreased the activity of these enzymes, resulting in a reduction of the seed and xanthotoxin yield in Ammi majus L. The higher seed and xanthotoxin yield achieved in Ammi majus L. at treatment T4 could be due to optimization of leaf soluble protein and photosynthetic rate, as these parameters are Influenced by S and N assimilation.

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; OD600 = 0.8), co-cultivation period (3 days), and acetosyringone concentration (100 μM) had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at 46℃ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.

The effects of angiogenesis inhibitor from the extract libraries of Korean and Chinese medicinal plants were investigated using a protein microarray chip. Protein chip was constructed by immobilization of integrin α5β1 on protein chip base plates and employed far screening active extracts that inhibit the integrin-fibronectin interaction from the extract libraries. The 100 extracts of medicinal plants were obtained from extract bank of National Institute of Crop Science, RDA. The 14 extracts among 100 extract libraries were shown efficient inhibition activity for the interaction between integrin-fibronectin. The medicinal plants of 14 extracts were Vitex negundo var. incisa (Lam.) C.B. Clarke, Epimedium koreanum Nakai, Cedrela sinensis A. Juss, Ipomea aquatica Forsk, Schisandra chinensis Baill, Pulsatilla koreana Nakai, Paeonia lactiflora Pall. var.hortensis Makino, Oenothera odorata, Allium chinense, Allium victorialis var. platyphyllum MAKINO, Polygonatum odoratum Druce var. pluriflorum Ohwi, Hosta lancifolia, Agrimonia pilosa L. var. japonica Nakai and Potentilla chinensis SER. The Paeonia lactiflora, Oenothera, and Agrimonia pilosa from these 14 extracts libraries were shown strong inhibition activity of integrin α5β1.

Oil and fatty acid composition of 648 soybean germplasms of different categories including Korean and American source were analyzed by NIRS (Near Infrared Reflectance Spectrophotometer) method at Yeongnam Agricultural Research Institute, Milyang, Korea. A

The effect of plant growth regulators was investigated on in vitro shoot proliferation from axillary bud explants of Hypericum erectum. To determine the optimal cytokinin for proliferation of axillay buds, we carried out screening four cytokinins (BA, kinetin, 2iP, TDZ). When nodal segments were cultured on MS medium supplemented with 4.5 μM TDZ (thidiazuron), a number of shoots were induced. Our results indicated that the addition of TDZ to culture medium resulted in the induction of significantly more axillary buds than in the addition of other cytokinins. The optimal concentration of TDZ for proliferation of axillary buds was 10 μM. 92% of shoots spontaneously rooted without any plant growth regulator (PGR) and formed whole plantlets within one month. More than 95% of these regenerants survived and they did not show any detectable variation in morphology or growth characteristics compared to their donor plants.

"Mirang", a new cultivar for vegetable soybean, was developed fi-om the cross between "Keunol" and "Geunjeongol"a black seed coat with black hilum and yellow cotyledon. Over 3 year of Korea Regional Adaptation Tests (2003-2005) for veg-etable soybean, Mir

A new light green seed coat Azuki bean cultivar, "Yeonkeum" was developed at the National Institute of Crop Science(NICS) and the Yeongnam Agricultural Research Institute (YARI) in 2005. Yeonkeum was selected from the cross between Jaja-sodun and "IT12099

"Danmi 2", a new cultivar for vegetable soybean, was developed from the cross between "Seokryangput" and"YS1307" (Seokryangput/Mi1yang77) at Yeongnam Agricultural Research Institute and released in 2005. The pedigree of'Danmi2", designated as Milyang 145

A new black seed coated soybean cultivar, "Ilpumgeumjeong 2" was developed at the Yeongnam Agricultural ResearchInstitute (YARI) m 2005. Ilpumgeumjeong 2 was selected &om the cross between "Milyang 68" and "Suwon 178". The prelim-inary, advanced and regio

A new soybean cultivar for tofu and soy-paste, "Daemang 2", was developed from the cross between 'Shinpaldal 2' and'Seokryang' by soybean breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2005. A promising line,SS93205-2B-8-3-2-1-1-1

Oil and fatty acid compositions of 1,429 germplasms including 1,121 cultivated soybeans for sprout production and 308wild soybeans were analyzed by the NIRS (Near Infrared Reflectance Spectrophotometer) method at Yeongnam AgriculturalResearch Institute, M

Suwon295 at the Honam Agricultural Research Institute (HARI) in 2005. An elite line, 953017-BG-BN-BN-53N, was selected in2000 and designated as Milyang126. It showed good agronomic performance in the regional adaptation yield trials (RYT) from2003 to 2005

Samkwangchal, a new covered waxy barley cultivar, was developed from the cros betwen Daebaegbori withearly maturing and large grain size and Suwon 302 with waxy endosperm by the Honam Agricultural Research Institutequality characteristics in the yield tri