‘보원’은 단경, 소분지 계통인 ‘SP8603-16-2-B(찌바한다치 /대광땅콩)’를 모본으로 하고 내병, 대립 특성을 가지는 대원 땅콩을 부본으로 하여 2001년도에 작물시험장에서 육성한 품 종이다. ‘보원’은 신풍초형(Arachis hypogaea ssp. fastigiata) 으로 키가 작고 지상부 생육 습성이 완전 직립형이다. 분지수 가 적고 꼬투리가 달리는 포기주변 반경이 작아 기계화 재배 에 유리하며 조숙형이다. 협당립수는 2립이고 종피색은 살색 이며 100립중이 ‘대광땅콩’보다 더 무거운 91 g으로 대립종 에 속한다. ‘보원’은 그물무늬병 저항성이 ‘대광땅콩’ 보다 강 하며 수확기의 낙엽정도가 대광땅콩보다 낮고 도복저항성도 ‘대광땅콩’ 보다 더 높다. ‘보원’의 수량성은 지역적응시험에 서 3개년간 평균수량이 3,720 kg/ha으로 ‘대광땅콩’ 보다 12 ～18% 증수를 보여 전국평균 17%의 증수를 보였고 연차간 및 지역간 변이가 ‘대광땅콩’ 보다 적어 수량안정성이 높다.

For storage periods of tubers in Chinese yam, the levels of ~textrmGA44 and ~textrmGA20 was constant, meanwhile both ~textrmGA53 and ~textrmGA19 level were always higher than that of ~textrmGA44 and ~textrmGA20 ㆍ ~textrmGA9 content as precursor of ~textrmGA4 was not changed during storage. ~textrmGA24 content was low to below 0.2 ng for 90 days after storage, ~textrmGA36 content as precursor of ~textrmGA4 like ~textrmGA9 was about 6-8 fold higher than that of ~textrmGA9 during storage. GA contents of the two gibberellin biosynthetic pathways were gradually increased when storage periods were progressed. Bioactive GA1 content as the GA members of an early C-13 hydroxylation was always constant, and its content was very low as below 0.1ng per dry weight, meanwhile, bioactive ~textrmGA4 content as the GA members of non C-13 hydroxylation was drastically increased, also, its content was highest at 90 days after storage, and then decreased at 120 days after storage. Consequently, we suggest that ~textrmGA4 may be involved in controlling tuber sprouting in Chinese yam.

The metabolism of [14~textrmC ] ~textrmGA12 in the Chinese yam (Dioscorea opposita Thunb. var. Tsukune) was examined to determine the identification of endogenous gibberellins. [14~textrmC ] ~textrmGA12 was metabolized to ~textrmGA53 , ~textrmGA44 , ~textrmGA19 , ~textrmGA20 , ~textrmGA1 , ~textrmGA8 , ~textrmGA15 , ~textrmGA24 , ~textrmGA9 , ~textrmGA36 and ~textrmGA4 . Radioactivity of GAs in non C-13 hydroxylation route was five-fold higher than that of early C-13 hydroxylation in analyzed GA-metabolites. Radioactivity of ~textrmGA4 was always four times higher than that of ~textrmGA1 at every feeding time. ~textrmGA1 radioactivity has always a lower level to below 200 DPM. The major pathway of endogenous GA metabolism in seedlings of the Chinese yam might be the non C-13 hydroxylation pathway.

Astragalus membranaceus has flowers that are similar to that of the legume family, but shows poor bearing when self-pollination is induced. Thus, this study was carried out observing the ripening procedure of pistils and stamens and development stages of pollen in the context of the birth and growth of the flower. As to the bearing of the flower of A. membranaceus, few pod setting and 13% pod setting were observed when self-pollination is induced by paper-bag covering or artificial pollination treated respectively. The result indicates that A. membranaceus is a cross-pollination plant. A pistil grew faster than a stamen until just before blooming. The flower size was about 17.0mm~times 4.0mm. Pistils and stamens had the same length after flowering. Pollen mother cells passed through meiosis and mitosis when its length reached around 3.5mm, thus creating the tetrade when 4 mm long. Pollen attained full growth when the bud was about 10mm long. An anther was found to tend to dehisce when the length of a bud reached around 12.0mm. As to the shape of pollen, about 70 % were normal. 1% and 30 % were small or empty pollen respectively. The result indicates that pollen of A. membranaceus attains full growth just before anther dehiscence which occurs before blooming while pistils grow faster than stamens until before flowering

This study was conducted to analyze the responses of some agronomic traits to the different cultural environments and relations among the agronomic traits for selecting sesame varieties with higher cultural stabilities. The indexes for stability parameters measured were coefficient of variability, heritabilities, genetic correlations and path coefficients of agronomic traits according to locations and years in Korea. The heritabilities of agronomic traits showed different by locations and years. Number of seeds per capsule and 1000 seeds weight showed higher heritabilities, but stem length and seed weight per plant showed relatively lower heritabilities. Average heritabilities of some agronomic traits in 1998 were comparatively higher than those of 1999. Of six areas, Jinju area showed biggest coefficient of yield variability in 1998-1999. Iksan and Taegu areas showed higher heritabilities in 1998, but Iksan and Jinju areas showed lower heritabilities in 1999. Genetic correlations were slightly higher than corresponding phenotypic correlations. Stem length showed positive genetic correlation with the number of capsules per plant, and seed weight per plant and the number of capsule per plant showed positive genetic correlation with seed weight per plant. On the analysis of path coefficients, stem length and number of capsules effected highly on grain yield. Great regional variations were observed on the effects of agronomic traits on grain yield. Higher direct effects of stem length on grain yield were observed at Suwon, Chungwon, Taegu, Jinju and Naju areas, but in Iksan area was observed higher direct effect of the number of capsules per plant on grain yield in 1998. In 1999, higher direct effect of stem length on grain yield was observed at Chungwon and Suwon areas. Iksan and Taegu areas were also observed higher direct effect of the number of capsule per plant on grain yield.

Volatile flavor compounds from perilla leaves were extracted and analyzed with different methods, head-space analysis (HS), simultaneous steam distillation and extraction (SDE) , and solvent extraction (SE), and to compare their efficiencies for quick analysis. Over 30 volatile compounds were isolated and 28 compounds were identified by GC/MSD. Major compound was perillaketone showing the compositions of which were 92% in SDE method, 86% in headspace analysis, and 62% in solvent extraction method. For quick evaluation of leaf flavor in perilla, it was desirable because the headspace analysis method had a shorter analyzing time and smaller sample amount than the other methods.

The sesaminol glucosides in 80% EtOH extract from sesame seeds were separated by high performance liquid chromatography (HPLC). A HPLC system using a Develosil ODS-5 column and gradient elution system from 30% to 80% methanol was selected for separation and quantitative determination of sesaminol triglucoside, sesaminol diglucoside, and sesaminol monoglucoside. Quantitative analyses for these sesaminol glucosides, sesaminol triglucoside, sesaminol diglucoside, and sesaminol monoglucoside were determined on the basis of standard curve of sesaminol glucosides. Sesaminol triglucoside, sesaminol diglucoside and sesaminol monoglucoside contents of the seed of one Korean sesame cultivar, Danbaekggae, were 56.4 mg/100g, 9.6 mg/100g, and 7.5 mg/100g, respectively. The most abundant aglycon of lignan glucosides in sesame seed was sesaminol triglucoside

Sesaminol in sesame seed was postulated to have antitumor activity. The present study was performed to characterize the role of crude sesaminol extracted from sesame seed (Sesame Crude Sesaminol; SCS) on inhibiting the in vitro growth of human leukemia HL-60 cells. SCS inhibited the growth of human leukemia HL 60 cells in culture and macromolecular synthesis in a dose and time dependent manner. The cytostatic range of SCS concentration was found to be 60 to 100 ~mu~textrmg /ml. SCS concentration greater than 200 ~mu~textrmg /mlwere cytocidal to HL-60 cells. When SCS concentraction was 6 ~mu~textrmg /mland 50 ~mu~textrmg /ml the synthesis of HL-60 cells was inhibited by 35% for DNA, 6% for RNA and 5% for protein and 83% for DNA, 76% for RNA and 60% for protein. Of specific interest was the irreversible effect of SCS in inhibiting DNA synthesis of HL-60 cells. This was evidenced from the fact that, even after washed with PBS three times, preincubated HL-60 cells still showed the inhibited DNA synthesis.

맥주맥의 육종에 있어서 곡립형질의 효율적인 선발자료를 얻고자 2조대맥 10품종을 공시하여 곡립형질의 유전력, 유전적 획득량, 상관관계, 경로계수등을 통계 유전학적으로 분석한 결과를 요약하면 다음과 같다. 1. 곡립형질들 중에서 곡피 (H=0.886), 분상질(H=0.848), 곡립형성비수(H=0.823)등은 유전력이 높고, 수량(H=0.526), 곡립폭(H=0.420)등은 유전역이 비교적 낮았다. 2. 기특되는 선발효율은 곡피(52.7%), 분상질(31.8%), 선입률(23.0%), 등이 높고, 곡립장(6.2%), 곡립폭(3.7%), 곡립후(8.7%) 등이 낮았다. 3. 맥주의 품질을 위하여 선발의 대상이되는 실용형질중 천립중, 분상질 Protein간의 상관관계는 다소유의성이 있었으나 곡립형질과 수량간의 상관관계는 유의성이 없었다. 4. 천립중, 곡피 및 수량의 선발은 이들에 대하여 직접효과가 큰 곡립후를 이용하여 간접적으로 선발하는 것이 효율적인 것으로 보였다. 5. 분상질 및 Protein을 위한 선발은 곡립후, 곡피 등과 수량간의 부의 간접효과를 개선하고 수량의 직접효과를 향상시키는 것이 유리할 것으로 보였다.