Diazotetracyanocyclopentadiene의 광화학적 분해반응 생성물을 저온 아르곤 및 크세논 매트릭스에서 적외선분광광도계법과 자외선-가시광선 분광광도계법으로 연구하였다. 저온 매트릭스에서 디아조화합물로부터 생성된 카르벤 중간체의 수득율은 낮았다. 디아조화합물을 광분해시켰을 때의 아레니우스 그림표는 온도변화에 직선적으로 변하지 않고 낮은 온도에서 tunneling효과를 보였다.

논토양 중에 벼의 근권에 서식하여 벼에 협력적인 관계로 질소를 공급해 주는 질소고정 미생물인 Klebsiella oxytoca를 미생물 비료로 활용하기 위한 새로운 질소고정 균주의 육종을 시도하여 다음과 같은 결과를 얻었다. 1. Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, Klebsiella oxytoca SH161에 pMC71A를 형질전환 한 경우, 무질소 배지에서 원래의 균주의 질소고정력에 비해 질소고정력이 각각 6.4배, 17.2배, 13.5배 증진되었다. 2. 15mM의 NH_4^+ 존재하에서, Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, Klebsiella oxytoca SH161의 경우는 모두 질소고정능의 완전한 저해를 받은 반면 pMC71A가 도입된 Klebsiella oxytoca NG13 /pMC71A, Klebsiella oxytoca SH31 /pMC71A, Klebsiella oxytoca SH161/pMC71A, 의 경우는 무질소 배지에서의 질소고정력에 비해 각각 13.7%, 7.7%, 6.2%의 질소고정 활성을 보였다.

토양에서 분리한 Streptomyces SP. 균주에서 분비되는 chitobiase의 효소학적 성질을 검토하였다. 최적반응조건은 pH 5.5, 40℃이었으며, 효소의 안정성은 pH 4.0∼5.5, 40℃에서 40분간 이었다. 45℃에서 1시간 열처리하였더니 효소의 활성은 거의 실활되었다. Mn^2+, Mg^2+이온에 의해 활성이 약간 증가하였으며 Fe^2+이온은 저해시켰으며, Km값과 활성화 에너지는 각각 1.5072mM, 8.314㎉/㏖이었다.

In order to improve the quality of Takju, the effect of combined use of Aspergillus oryzae and Aspergillus kawachii in brewing was investigated. The quality of Takju which was brewed by the combined use of Aspergillus orytae CF7-koji and Aspergillus kawachii CF1-koji in equal amount was better as compared with that brewed by existing method using Aspergillus kawachii-koji only as koji. But the good result did not obtained when the koji was made by mixed culture of Aspergillus CF7 and Aspergillus kawachii CF1.

Lignocellulose and lignolic compounds were absolutely given much weight in the biosphere, and their degradation was essential for continuous biological carbon circulation, whereas aerobic cellulolytic microorganism dissolved the cellulose into their elements in the first stage, strict anaerobic cellulolytic microorganism`s role was taken a increasing interest through the recent research. It was reviewed that anaerobic microbial degradation process of lignocellulose and its derivatives (celljlose, lignin, oligolignol and monoaromatic compound), and function of anaerobic microorganism on the environmental ecology.

This study was carried out to investigate the effect of the emulsion stability according to the quantity of emusifiers and the pressure of homogenizer in coffee whitener preparation. The results of this study are as follow : 1. To obtain a standard emulsifier, the ratio of mono and diglycerides (Atoms 150), sorbitan monostearate (Span 60) and polyoxylene(20) sorbitan monostearate (Tween 60) was 60 : 20 : 20 and the optimum quantity was about 0.9% for stable emulsion. 2. Characteristics as coffee whitner were markedly improved when the pressure of homogenizer was 200∼250㎏/㎠ and homogenizing times are over 4. 3. The good whitening capacity was showed when fat glouble`s average diameter was under 0.5㎛.

This study was conducted a comprehensive sarvey of 39 elementary schools operating school lunch program in Seoul area. The purpose of this study was to investigate the realities of school lunch program. This method of this research was based on the interview survey with dietitian working at each school with prepared questionaire. The survey was conducted for 16 days from Dec. 5 to Dec. 20., 1990. The results of this study were as follows ; (1) The average numbers of children supplied with food were 1, 244 for each school. It was about 44.3% of the students enrolled in the school. The average feeding cost was 738 won per a child for a day. (2) Only one school was operating nutritional education as a regular educational program, and others were operating nutritional education off and on. (3) All nutrient intake except energy were higher than the RDA for school lunch program. (4) The ratio of animal food was 46.2%, and that of vegetable food was 53.8%. (5) The ratio of schools without sterilizer cabinet came to 56.4%, and that of schools without warmer was 97.4%. (6) The facilities for drainage, lighting, ventilation, anti-rat of a cookery were comparatively good.

This study was divised to observe an inhibitory toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng (NPG : Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L is known to be a lipolytic factor that was partially purified from the ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol precipitate component of large root NPG(mean 46.8%) was higher (mean 1.8 times) than that of water extract component in final reaction concentration ; 500㎍/㎖, on the other side inhibitory effect of water extract component in small root NPG (mean 43.9%) was higher(mean 1.2 times) than that of ethanol precipitate component, respectively. In a way inhibitory effect of ethanol precipitate component in large root NPG(47.6%), when final reaction concentration of sample were l, 000㎍/㎖, was about 40% lower than that of Korean red ginseng, respectively.