Bumblebees are important pollinators in greenhouse and have colonized all parts of the World. In Korea, the value of bumblebees is increasing as pollinator. However, the more recent use of reared colonies may ultimately allow pathogens to spread to peripheral areas for bumblebees. Generally, bumblebees are hosts to a large number of parasites which are mites, flies, protozoa, fungi, virus and bacteria. For most of these, very little is known about their effects against host insect, epidemiology or evolutionary ecology. So, we report pathogenic bacteria isolated from Bombus terrestris and B. ignitus at first time in Korea. Bacillus fusiformis and Klebsiella oxytoca are isolated from B. terrestris and confirmed with 16S rRNA gene nucleotide comparison in NCBI genebank. Pathogenicity of B. fusiformis was 35~40% against B. terrestris. Pantoea dispersa and K. oxytoca are isolated from B. ignitus and confirmed with 16S rRNA gene nucleotide comparison in NCBI genebank. Pathogenicity of these species were 35~40% against B. ignitus. These pathogenicity are considered as low-level.

A new species, Hatchiana n. sp., is confirmed by morphological and molecular data. The genus Hatchiana Fender, 1966 belonging to Podabrini LeConte, 1881 is distinguished by the shape of tarsal claws: all tarsal claws with blunt tooth in both sexes. The genus was recognized as a subgenus of Podabrus Westwood, 1838. Recently, however, Hatchiana was suggested as separated genus. The genus Hatchiana was known as 12 species in Palaeartic region including three species from Korea. In this study, we found Hatchiana n. sp. from several areas of Taean-gun, Chungcheongnam-do, Korea. This new species is different from closely relative species, Hatchiana jirisanensis (Kang & Kim, 2000) by following morphological characters: size of compound eye, length of antennae, shape of pronotum, shape of scutellum, and shape of aedeagus. Also, we compare the DNA barcoding region (the former region of CO1 gene) between these two species as molecular characters. In result, Hatchiana n. sp. is distinct from Hatchiana jirisanensis Kang & Kim by discrepancy of three percents in CO1 sequence. Therefore, the Korean Hatchiana is confirmed as four species, adding Hatchiana n. sp.

글루코오즈(GOD) 옥시다제가 고정화된 PVA/키토산 플렌드막과 다공성 폴리아미드 복합막을 통해 인슐린의 투과거동을 살펴보았다. GOD가 고정화된 막을 통한 투과계수는 10-6~10-7cm3/cm2sec이었다. 복합막의 클루코오즈 농도에 대한 변화는 낮은 글루코오즈 농도에서 높았는데 이는 막으로부터 산소의 고갈 때문이었다. PVA/키토산 및 다공성 폴리아미드막을 통한 인슐린의 투과는 글루코오즈 농도에 따라 500mg%까지 점차 증가하였다.

치과 콘빔 전산화단층 검사 시 갑상선 부위의 방사선 피폭선량을 최소화하기 위하여 보루스 (Bolus)를 이용한 차폐체를 제작하고, 방사선 차폐 효과와 영상의 적정성을 평가하였다. 치과용 콘빔 전산화단층검사 장비를 사용하여 치과방사선 두부 팬텀을 대상으로 갑상선의 좌, 우측 부위에 유리선량계를 부착시켜 방사선량을 측정하였다. 차폐체의 두께를 각각 10 mm, 20 mm, 30 mm로 다르게 하여 각 차폐체별로 흡수선량을 측정하여 차폐체를 사용하지 않았을 경우와 비교하였다. 8명의 평가자가 진료영상의 적정성을 여부를 평가하였다. 왼쪽 갑상선 부위에 30 mm Bolus 차폐체를 사용한 경우에는 결과값이 평균 342.67 μ㏉로 Bolu s 차폐체를 사용하지 않고 측정한 결과값의 평균 431.22 μ㏉보다 20.7% 감소하였고, 오른쪽 갑상선 부위에 30 mm Bolus 차폐체를 사용한 경우에는 평균 424.56 μ㏉로 21.9%의 선량감소효과를 보였다. 진료 영상의 적정성은 평가자 모두 사용 가능하다 판단하였다. 결론적으로, 치과 콘빔 전산화단층검사 시 갑상선 부위의 방사선 피폭선량을 최소화하기 위해 제작된 Bolus 차폐체는 장해음영 없이 적정한 진단 영상 처리가 가능하고, 방사선차폐 효과가 있어 유용한 차폐체로 적용 가능할 것으로 사료된다.

Background : Ixeris genus has been used in traditional medicines as stomachics, sedatives, and diuretics. Ixeris dentata var albiflora is a kind of perennial herbaceous plant and one of the plants of the genus Ixeris (Asteraceae). It is well-known for edible wild vegetable in Korea, China, Japan, and Mongolia. Specially, Korean has its root and young leaf with appetizing vegetable due to bitter taste. Methods and Results : We isolated 8 genes that are involved in carotenoid biosynthesis using the Illumina/Solexa HiSeq2000 platform. In this study, a full-length cDNA clone encoding phytoene synthase (IdPSY), phytoene desaturase (IdPDS), ξ-carotene desaturase (IdZDS), lycopene β-cyclase (IdLCYB), and zeaxanthin epoxidase (IdZEP) and partial-length cDNA clones encoding lycopene ε-cyclase (IdLCYE), ε-ring carotene hydroxylase (IdCHXE), and β-ring carotene hydroxylase (IdCHXB2) were identified in I. dentata. The theoretical molecular weight (MW) and isoelectric point values of 8 genes were investigated. Sequence analyses revealed that these proteins shared high identity and conserved domains with their orthologous genes. IdPSY, IdPDS, IdZDS, IdLCYB, IdCHXB2, and IdZEP were constitutively expressed in the roots, stems, leaves, and flowers of I. dentata. Conclusion : Our study on the biosynthesis of carotenoids in I. dentata will provide basic data for elucidating the contribution of carotenoids to the considerable medicinal properties of I. dentata.

Background : The Aralia cordata and Dendropanax morbifera, belonging to the family Araliacea, is a perennial herbaceous species. Although its beneficial effects for several chronic diseases are well-known, the role of its effects on chronic intestinal inflammation, colon carcinogenis are limited. Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of gastrointestinal tract. It has been reported that IBD is associated with colon cancer which is one of the most common types of cancer diagnosed and a major cause of cancer-related mortality. Methods and Results : The present study hypothesized that Araliacea family would exert a protective effect on inflammation and dextran sulfate sodium (DSS)-induced colitis mouse model. Effect of Aralia cordata extracts (ACE) and Dendropanax morbifera leaf extracts (DMLE) on pro-inflammatory markers, mitogen-activated protein kinase signaling (MAPKs), activation of nuclear factor κB (NF- κB) in THP-1 human monocyte and HT-29 colon epithelial cell model were investigated. In addition, colon inflammation inhibitory effects of ACE and DMLE on the characteristics of in DSS-induced colitis model. Results showed that ACE, DMLE attenuated the severity of DSS-induced colitis, as assessed by disease activity index scores, shrinkage of colon length, and histopathologic changes and infirmatory cytokine concentration. Furthermore, ACE treatment significantly suppressed the colon carcinogenesis in AOM/DSS induced colon cancer model. The apoptosis induction abilities of the DMLE were studied by analyzing the expressions of Bcl2 family protein, caspase, Annexin V/PI staining and DNA fragmentation. According to our results, DMLE significantly induced cell death in SW-480 cells. Apoptosis was determined by cell morphology and electrophoresis of DNA fragmentations. Furthermore, treatment with DMLE also induced the increase in caspase activity, pro-apoptotic proteins (Bax and Bad) and the decrease in anti-apoptotic proteins (Bcl-2 and Bcl-xL). In addition, DMLE induce cell cycle arrested at the G1/S phase in SW-480 cells and exhibited significant inhibition on the expressions of G1/S phase specific CDK, cyclin and up regulation of CDK inhibitors. Conclusion : Taken together, these results provide evidence that ACE and DMLE prevention colon cancer by regulating inflammation, colitis, and colon carcinogenesis which indicates its benefit for colon health.

국화 6 품종을 대상으로 감마선(35 Gy) 조사에 의해 유기 된 화색변이체를 선발하고, 식물체 재생을 위한 배지내 식물생 장조절제 적정농도를 구명하여 신품종 육성 가능성을 검토하고 자 수행하였다. 고유품종과 다른 꽃잎의 화색변이율은 ‘SP-410’ 이 15.7%로 가장 높았으며, ‘예스스타’가 10.6%, ‘무지개’가 10.3%순 이었다. ‘무지개’는 이중색(황색/적색, Y7D/R40A) 에서 적색(R42B)으로, ‘예스라인’은 자주색(RP74C)에서 진자 주색(RP63B)으로, ‘예스스타’는 황색/적색(Y4A/RP59A) 이 중색에서 적자주색(RP64A)으로, ‘SP-410’은 백색(W155C)에서 황색(Y2C)으로, ‘예스투게더’의 화심색은 백색(W155C)에서 연보라색(P76B)로, ‘예스우리’의 화심색은 연녹색(YG149A)에 서 짙은 녹색(GY1C)으로 화색이 변한 변이체를 선발하였다. 화색변이체 꽃잎의 신초형성을 위한 배지내 식물생장조정제 의 최적조합은 BA 1.0 mg/L와 NAA 0.5 mg/L, BA 2.0 mg/L 와 NAA 0.5 mg/L의 2조합이였으며, 신초형성율은 품종간에 차이가 있어 ‘예스라인’이 91.6%로 가장 높았다. 감마선 조 사에 의한 화색 변이체의 꽃잎배양 유래 재생식물체는 영양 번식으로 유지되었으며, 3년동안 재배한 결과 화색과 꽃잎의 형태적 특성 차이가 없었다.

농촌진흥청 국립원예특작과학원에서는 적색 스탠다드 "Francesco" 품종을 모본으로, 보라색의 스탠다드 "Saturn"를 부본으로 1996년에 인공교배하여 얻은 실생계통으로부터 시들음병 저항성이 강한 자주색 대형 절화용 스탠다드 카네이션을 개체 선발하였다. 1997년부터 2000년까지 생육특성 검정 및 품종평가회를 통한 소비자 기호도 평가를 검정하고 2000년에 농작물 직무육성 신품종 선정 심의회에서 우량품종으로 선정되어 분홍색 스탠다드 "자수정" 품

저장조건을 달리한 딸기 화분을 FCR Test와 결실률을 바탕으로 하여 화분 활력을 조사하고 암술 주두 활력을 인공수분을 통하여 구명함으로서 딸기 교배 육종시 효율성을 높이고자 하였다. '설향'을 대상으로 화분 활력을 조사하였으며 암술 주두의 수정 능력은 '매향' 등 4품종을 공시하고 화분친은 '설향'을 사용하였다. 딸기 화분은 상대습도 33%이하의 건조한 조건에서는 처리 온도(18℃ 수식 이미지 및 22℃ 수식 이미

A new poinsettia cultivar 'Scarlet' was bred by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Christmas Eve', a variety with dark green leaves and dark red bracts, and 'Red Velvet', a red colored variety in 2003. 'Scarlet' was finally selected in 2006 after investigating the growth and flower characteristics from 2004 to 2006. 'Scarlet' has bright red colored and elliptic bracts and has absent or very weak intensity of rugosity between veins in bract. In comparison with the check variety, 'Red Velvet', plant height is tall, leafblade is long and narrow. Petiole is also longer than that of 'Red Velvet'. Short day response is fast and its bracts are fully colored 7 weeks after short day commencement.

A new poinsettia cultivar 'Miss Maple' was bred by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Sonora Red', a variety with short plant height, dark green leaves and dark red bracts, and 'Cranberry Punch', a deep pink colored variety in 2003. 'Miss Maple' was finally selected in 2006 after investigating the growth and flower characteristics from 2004 to 2006. 'Miss Maple' has light red colored and obovate bracts, and has weak intensity of rugosity between veins in bract. Fully colored transitional leaves are so deeply lobed that they resemble the leaves of maple. Plant height is shorter than that of 'Cranberry Punch', the check variety. Short day response fast and its bracts are fully colored 7 weeks after short day commencement.

A new standard rose cultivar ‘Pink Stone’ was bred from the cross between red standard cultivar ‘Red Queen’ and pink standard cultivar ‘Vivaldi’ at the National Horticulture Research Institute. The cross was made in 2001 and ‘Pink Stone’ was finally selected in 2006 after investigating characteristics for three years from 2003 to 2006. ‘Pink Stone’, a deep pink standard cultivar grows vigorously and has powder mildew resistant. The major characteristics of this cultivar are 115.1 stems/m2/year in yield, 77.7 cm in length of cut flower, 10.5 cm in flower diameter, 67.0 in petal number, and 12.0 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer’s preference of this cultivar is relatively higher than that of control cultivar, ‘Noblesse’.