Background : This study is designed to select the best shading materials for the 4-year old ginseng K-1 variety by examining the light volume, photosynthesis and growth characteristics of ginseng. Methods and Results : The ginseng seedling was transplanted in the 7 lines x 9 rows (63 plants)/ 1.65 ㎡. The shading materials were installed in the form of rear line with 170 ㎝ high for front pole and 100 ㎝ high for rear pole. The installation of shading materials were installed in combination of polyethylene, blue polyethylene sheet + polyethylene, polyethylene + polyethylene, polyethylene + aluminium screen 70%, polyethylene + aluminium screen 100%, aluminium shade plate to inspect the photosynthesis and ginseng's growth characteristics. The photosynthetic rate was measured on the middle of small leaf using the photosynthetic tester (L1-6400, Li-COR) a t 10 : 00 – 14 : 00 on a clear day with the light volume of 100, 200, 400, 600, 800, 1,000, 1,400, 1,800 μmol s-1m-2.For the ginseng K-1, which is 4-years old, the aerial parts growth (such as survival rate, height of plant, length of stem, diameter of steam and length of leaf) and underground part (length, diameter and weight of root) were examined in 2016. As for the PAR (photosynthetically active radiation), the aluminum shade plate showed the best performance while the polyethylene + polyethylene showed the least. As for the photosynthetic rate, the following order was shown in the decreasing order: blue polyethylene sheet + polyethylene > polyethylene > aluminium shade plate ≒ polyethylene + aluminium screen 100%, > polyethylene + polyethylene > polyethylene + aluminium screen 70%. As for the survival rate for 4-year old ginseng, the polyethylene + aluminium screen 70% showed the highest survival rate with 91.0%. As for the growth of aerial part of ginseng, the polyethylene and polyethylene + polyethylene showed the best growth. As for the underground part of ginseng, the polyethylene, blue polyethylene sheet + polyethylene, and polyethylene + polyethylene showed the best growth in the length of roots and length of main root while polyethylene and blue polyethylene sheet + polyethylene showed the best growth in the weight of roots. Conclusion : In conclusion, considering the characteristics of photosynthesis and the underground parts of ginseng, it was found that the best shading materials for K-1 4-year old ginseng is blue polyethylene sheet + polyethylene.

Background : The study is designed to select the organic fertilizer as an additional fertilizer suitable for improving the yield of ginseng cultivar K-1 at low productive land when it is cultivated as 6-year old red ginseng. Methods and Results : This study was conducted in 2016 at a farm in Paju cultivating 6-year old ginseng root. As for the management of the cultivation land, in 2010 and 2011, Sudangrass was cultivated in order to make the organic matter content and chemical composition suitable for ginseng cultivation, and more than 15 tillage works were carried out a year. The transplantation was carried out at the planting density of 63 weeks / 1.62 ㎡ in April 2012. On May 2, the amount of 15 ℓ / 1.62 ㎡ of N 1.5 ㎏/1,000 ㎡ was supplied to the non-treatment, liquid fertilizer of fermented rice bran, liquid fertilizer of fermented soybean, seaweed and liquid yeast. N 1.5 ㎏/1,000 ㎡ was put into the soil for the fermented soybean and leaf mold. The photosynthetic rate was measured on the middle part of the small leaf by using a photosynthetic analyzer (LI-6400, Li-COR) at 10 : 00 - 14 : 00 on a clear day under PAR (photosynthetically active radiation) of 100, 200, 400, 600, 800, 1,000, 1,400, 1,800 μmol s-1m-2. The growth features of ginseng (including the length of stem, length of leaf, width of leaf, length of root and yield, etc.) as well as leaf discoloration, and root rot, etc were examined. The statistical analysis was performed 3 times in the randomized block design. For the rate of photosynthesis, the liquid fertilizer of fermented rice bran > liquid fertilizer of fermented soybean ≒ untreated ≒ fermented soybean > seaweed > liquid yeast > leaf mold was higher in that order. The survival rate ranged from 42.9% to 51.9%. As for the above-ground growth characteristics, the liquid fertilizer of fermented rice bran, liquid fertilizer of fermented soybean, seaweed and liquid yeast showed higher performance than the non-treated while the fermented soybean and leaf mold showed similar performance to the non-treatment. As for the underground growth, the liquid fertilizer of fermented rice-bran, liquid fertilizer of fermented soybean, fermented soybean, or leaf mold showed the same performance as non-treatment while the fermented soybean or leaf mold showed shorter length than non-treatment. As for the yield, the liquid fertilizer of fermented rice-bran, or liquid fertilizer of fermented soybean showed better performance than non-treatment. Conclusion : In conclusion, it was found that liquid fertilizer of fermented rice bran and liquid fertilizer of fermented soybean are the suitable organic fertilizers which can be used in ginseng cultivation for improving the quantitative yield of 6-year-old ginseng in low-producing area.

In order to determine the short-term impact induced by chloride ionic, CaCl2 was used to study the chloride ionic effects of salinity on substrate and growth of Dracacena braunii grown in ornamental hydro-culture. A distilled water (control) was enriched with 10, 20, 50, 100, and 150 g･L -1 of CaCl2, respectively. Before planting, acidity and electronic conductivity values remarkably increased with increasing concentration of CaCl2. However, 4 weeks after planting, acidity values decreased to a slightly acidic pH, while there were not significant differences among electronic conductivity values obtained. Number of root, fresh weight, and total chlorophyll content were significantly decreased in response to CaCl2 concentration in comparison with control, whereas dry weight, water content, and color of stem were no significant effect of CaCl2 concentration less than 20 g･L -1 . These results showed that initial CaCl2 concentrations above 20 g･L -1 is considered to be the threshold value that will sustain the Dracacena braunii in the growth condition and above which plant growth will be retarded.

On June 14, 2008 (the first experiment) and July 24, 2008 (the second experiment), the shores of the Boseong River and the sandy beaches, Seokgok-myun, Moksadong-myun, Gokseong-gun in Jeollanam Province were investigated and a total of 29 soft-shelled turtle (Tryonyx sinensis) eggs in the natural spawning nest eggs were collected (13 eggs were collected in the first experiment and 16 eggs in the second experiment). The temperatures in the natural spawning nests were 25.9- 36.9±0.5℃, the depth of the eggs was 5.2-7.5±0.5 cm as the distance of the average 6.4±0.5 cm. 29 eggs were scattered at least 0.2 cm interval. Artificial incubation of 29 eggs was conducted in artificial nest boxes in thermo-plastic composition of the incubator, and then incubated at 26.5-35.5±0.5℃, and an average constant temperature was 31.2-32.1±1.0℃. The incubation days ranged from 53 to 55. In case of most turtles, incubation at 31℃ (higher temperatures) generally produces all or mostly females, while incubation at 25℃(cooler temperatures) produces all or mostly males. Exceptionally, in case of genus Trionyx, the sex ratio of female : male of T. sinensis of a freshwater soft-shelled turtle was approximately 1:1, which differs from other genera of turtles and makes T. sinensis Strauch only turtles presently known to lack temperature-dependent sex determination.

Background : Tooth vitality is reflected by the health of dental pulp. Schisandrin C is a natural compound extracted from the fruit of Schisandra chinensis which has anti-inflammatory and anti-oxidant properties. The role of Schisandrin C on human dental pulp cells (HDPCs) has not been studied yet. This study examined the properties of Schisandrin C as an anti-inflammatory and anti-oxidant compound, and whether its characteristics promote mitochondrial biogenesis in HDPCs. Methods and Results : HDPCs were extracted from fresh third molars and cultured. Reactive oxidative stress (ROS) and nitric oxide (NO) formation were analyzed by a Muse cell analyzer. Western blotting and gelatin zymography were used to identify the presence of anti-oxidants, as well as inflammatory and mitochondrial biogenesis. Confocal microscopy was used for the detection of mitochondrial activity. Schisandrin C inhibited lipopolysaccharide (LPS)-stimulated inflammatory molecules; intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 and -9 (MMP-2/9), NO production, ROS formation and the mitogen-activated protein (MAPK) pathway through minimizing the nuclear factor kappa B (NF-kB) translocation. Schisandrin C increased the expression of superoxide dismutase (SOD) enzymes as well as heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) through the phosphorylated-protein kinase B (p-AKT) and nuclear factor erythroid 2-related factor-2 (Nrf-2) pathways. The anti-inflammatory and anti-oxidant properties of Schisandrin C promoted mitochondrial biogenesis. Conclusions : These results suggest that Schisandrin C may be used as an anti-inflammatory compound to reduce oral inflammation such as pulpitis.

Cytological changes of the epithelial cells according to the developmenatal phases of the seminal vesicle related to the spermatogenic stages in the testicular lobules during spermagenesis in male Neptunea (Barbitonia) cumingii (Gastropoda: Buccinidae) were investigated monthly by electron microscopical and histological observations. N. (B) cumingii is dioecious, and an internal fertilization species. The male genital organ is located near the tentacles. The spermatozoon is approximatley 50 μm in length. The axoneme of the tail flagellum consists of nine pairs of microtubles at the periphery and one pair at the center. The process of germ cell development during spermatogenesis can be divided into five succesive stages: (1) spermatogonia, (2) primary spermatocytes, (3) secondary spermatocytes, (4) spermatids, and (5) spermatozoa. A considerable amount of spermatozoa make their appearance in the testicular lobules (or acini) and some of them are tranported from the testis towards the seminal vesicles until late July. In this study, the developmental phases of the epithelial cells of the seminal vesicles of N. (B.) cumingii could be classified into four phases: (1) S-I phase (resting), (2) S-Ⅱphase (early accumulating), (3) S-Ⅲ phase (accumulating), and (4) S-IV phase (spent). However, in case of N. (B.) arthritica cumingii, the developmental phases of the seminal vesicle were devided into three phases: (1) resting, (2) accumulating and (3) spent. Granular bodies in the inner layer of the seminal vesicles are involved in resorption of digestion of residual spermatozoa.

Ultrastructural studies on oocyte development and vitellogenesis in oocytes, and the functions of follicle cells during oogenesis and oocyte degeneration were investigated to clarifyb the reproductive mechanism on vitellogenesis of Scapharca subcrenata using electron microscope observations. In this study, vitellogenesis during oogenesis in the oocytes occured by way of autosynthesis and heterosynthesis. Of two processes of vitellogenesis during oogenesis, the process of endogenous autosynthesis involved the combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum. However, the process of exogenous heterosynthesis involved endocytotic incorporation of extraovarian precursors at the basal region of the oolema of the early vitellogenic oocytes before the formation of the vitelline coat. In this study, follicle cells, which attached to the previtellogenic and vitellogenic oocytes, were easily found. In particular, the follicle cells were involved in the development of previtellogenic oocytes by the supply of nutrients, and vitellogenesis in the early and late vitellogenic oocytes by endocytosis of yolk precursors. Based on observations of follicle cells attached to degenerating oocytes after spawning, follicles of this species are involved in lysosomal induction of oocyte degeneration for the resorption phagosomes (phagolysosomes) in the cytoplasm for nutrient storage, as seen in other bivalves. In this study, the functions of follicle cells can accumulate reserves of lipid granules and glycogen particles for vitellogenesis from degenerating oocytes after spawning.

Background : Cordyceps militaris has been an wonder drug to anti-aging efficacy and called the three main drugs with ginseng and deer antler from the past. Cordycepin, cordycepic acid (d-mannitol) and adenosine are known as functional ingredients in Cordyceps militaris. Among them, cordycepin, the representative component, has been reported as antimicrobial substance containing immune enhancement, anti-cancer and anti-inflammatory effects. Methods and Results : After Cordyceps militaris produced from different types of medium mixed with 10-fold volume of purified water, the mixture were extracted at 70±5℃ for a hour and that extracts re-extracted using ultrasonics wave for 30 minutes. Qualitative analysis of the index component was determined by using the Q-TOF (A quadrupole time-of-flight mass spectrometer), and quantitative analysis was performed by using HPLC (high-performance liquid chromatography) with Xselect HSS T3 column (2.1 X 100 mm, 2.5㎛, Waters, USA) and ultrapure water and acetonitrile as mobile phase A and B. Detection column temperature, injection volume and the flow rate were 35℃, 2 μL and 0.3 mL / minute respectively. The cordycepin content of Cordyceps militaris produced from medium mixed with vegetable and animal ingredients higher than single ingredient. Moreover, through a variety of analyzes by varying the type and content of the medium additives, the cordycepin in Cordyceps militaris produced from medium mixed with animal ingredients highest. Furthermore, the cordycepin content of a fruit body was higher than those of the a mycelium. Conclusion : These results provide a method for producing an high cordycepin content of Cordyceps militaris as functional food ingredient.

Purpose – This study aims to analyze time use of employees to see how they make a balance between work and family. We tried to analyze time use and time pressure of married paid workers in order to understand their work and family balance. Methodology – Time use was compared by employment types and time pressure groups. We analyzed the factors influencing time pressure, dividing two employment types. The data were selected from the 2009 Korean Time Use Survey. Results – Those who feel time pressure among full-timers spent more time on work and housework and less time on personal care and leisure than those who do not feel time pressure. Logit analysis on time pressure showed that full-timers feel more time pressure than part-timers do. Gender, age, education, income, day-off type, and the presence of preschool children were significant variables on time pressure. Conclusion – These results imply that time use and time pressure for married paid workers are affected by employment types. Flexibility of labor needs to enhance work and family balance for females who have preschool children.

This study was examined the ovogenesis of Ussurian bullhead, Leiocassis ussuriensis and the morphological development of its larvae and juveniles and to use the results as basic information for the preservation of species and resource enhancement. For artificial egg collection, human chorionic gonadotropin (HCG) was injected at a rate of 10 IU per gram of fish weight. During breeding period, water temperature maintained at 24.5~26.5℃ (mean 25.0±0.05℃). The process of ovogenesis reached the two-cell stage in 50 minutes after fertilization. In 73 hours of fertilization the movement of the embryoid body became active state and the larvae began to hatch from the tail through the oolemma. Length of prelarvae were 6.33~6.50 mm long (mean 6.40±0.06 mm) just after hatching having yolk with their mouth not opened. After thirty eight days of hatching, juveniles were 30.6∼32.5 mm long (mean 31.5±0.65 mm). The color was dark yellowish brown throughout the entire body, and the number of caudal fin rays developed to thirty six perfectly.

Enhancing yield has been a major challenge of agriculture. In rice, tiller number is one of the important biomass and yield components. A maize mutant grassy tillers1 (gt1) increases lateral branches in maize. The GT1 gene encodes a class I homeodomain leucine zipper (HD-Zip) protein. In maize, the gt1 expression is induced by shading and is dependent on the activity of teosinte branched1 (tb1), a major domestication locus controlling tillering and lateral branching. To estimate the biological role and agricultural utility of gt1 in rice, rice homologue (OsGT1) has been isolated and its overexpressors and RNAi lines were generated. Field data showed that OsGT1 overexpressors reduced tillers and panicles while RNAi lines increased them, compared to wild type. Shade signal is an important factor in determining lateral branching. To understand the relationship between OsGT1 and shade avoidance, plants have been grown under 50% shading in the field. Also, double genetic combinations with phytochrome mutants (phyA, B, and C) are being examining for tillering phenotype. These ongoing researches will provide insights in determining the action of OsGT1 on branching and shade avoidance in rice.

Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in the severe loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is required to assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolated from Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. The expression of BrTSR53 was determined by quantitative real-time PCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stress treatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a gene that confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was cloned into an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blot analysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stresses compared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important genetic resource for abiotic stress resistance.

The purpose of this study was to evaluate the applicability of the microbial fuel cell for the combined treatment of food waste water and landfill leachate. Contents of the study was to develop a carbon-containing electrode material radially to maximize microbial attachment. Also to evaluate the electric energy production efficiency by combining the electrode surface coating technology. By using a microbial fuel cell organic matter and nitrogen removal efficiency is evaluated for the food waste water and landfill leachate. BET to evaluate the surface characteristics of the developing electrode (Brunauer Emmett Teller) To evaluate the coating adhesion through measurement and to evaluate the adhesion characteristics micro-organism Weighing. Excellent electrical conductivity in the development electrode platinum, cobalt, by coating a catalyst such as palladium and to evaluate the electric energy generation efficiency. Lab. scale reactor capacity is a 5 L, and to configure the cross-section and the oxidizing electrode as cathode sequentially added.

Scouring of bridge foundation is one of the major cause of bridge failure. Scour can be defined as the excavation of foundation or other material from the bed and banks of streams, due to water flow. Scour monitoring is one of the major requirements to ensure bridge safety. There are some underwater instruments such as float-out devices which are used in scour monitoring. The available conventional underwater instruments are expensive and difficulties in maintenance. Thus vibration based monitoring techniques are emerging, this paper is one of such effort. This paper develops a vibration-based scour monitoring technique. The effect of sour on the vibration characteristics of pier is not significant at the early stages of scouring but significant changes in vibration characteristic can be identified during moderate level of scour. Thus this method can be used to identify and alert the safety of bridge prior its failure. An unscented Kalman filter is employed in this process. This paper numerically validates the monitoring capability of developed method over other vibrations based methods.

Ultrastructural studies of oocyte degeneration in the oocyte, and the functions of follicle cells during oocyte degeneration are described to clarify the reproductive mechanism on oocyte degeneration of Mactra chinensis using cytological methods. Commonly, the follicle cells are attached to the oocyte. Follicle cells play an important role in oocyte degeneration. In particular, the functions of follicle cells during oocyte degeneration are associated with phagocytosis and the intracellular digestion of products. In this study, morphologically similar degenerated phagosomes (various lysosomes), which were observed in the degenerated oocytes, appeared in the follicle cells. After the spawning of the oocytes, the follicle cells were involved in oocyte degeneration through phagocytosis by phagolysosomes. Therefore, it can be assumed that follicle cells reabsorb phagosomes from degenerated oocytes. In this study, the presence of lipid granules, which occurred from degenerating yolk granules, gradually increased in degenerating oocytes. The function of follicle cells can accumulate reserves of lipid granules and glycogen in the cytoplasm, which can be employed by the vitellogenic oocyte. Based on observations of follicle cells attached to degenerating oocytes after spawning, the follicle cells of this species are involved in the lysosomal induction of oocyte degeneration for the reabsorption of phagosomes (phagolysosomes) in the cytoplasm for nutrient storage, as seen in other bivalves.

The egg development and early life history of Korean spotted sleeper, Odontobutis interrupta which is Korean endemic species from Sora-choen was investigated. The Korean spotted sleeper were caught at Sora-myeon, Yeosu-si, Jeollanamdo, from Korea at May in 2014. The fertilized eggs were 4.23 ± 0.05 mm in long diameter and had oil globules. Hatching time of the embryo began about 442 hr 14 min after fertilization under water temperature of 19.5oC. The newly hatched larvae were 4.27 ± 0.35 mm in total length and their anus were not yet opened. 3 days after hatching postlarvae was measured 6.20 ± 0.11 mm in total length. 10 days after hatching postlarvae was measured 6.69 ± 0.14 mm in total length.

Primary progressive aphasia (PPA) is a progressive degenerative disorder that primarily affects language. Although the etiology of PPA has not been determined, autoimmune mechanism is known to be one possible pathophysiology. We investigate efficacy of steroid treatment in a patient diagnosed as both logopenic variant PPA and Sjögren's syndrome. After steroid treatment, her cognitive function, especially language ability showed improvement. And also, FDG-PET showed metabolic increase in the bilateral temporal lobe. However, on reassessment after stopping steroid treatment, her cognitive performance declined to pretreatment level.

The PepMoV has been considered the most frequently detected potyvirus. When it co-infects with CMV or PMMoV, it gives severe impact to total pepper harvest in Korea. Since F1 hybrid that resistant to PepMoV has not been developed, we have developed transgenic peppers using Agrobacterium-mediated transformation with a Hc-Pro gene of the PepMoV. A large number of GM peppers were tested for resistance to the PepMoV, and after consequent self-crossing up to T4 generation, a highly tolerant pepper to PepMoV called T20 was selected. So far, BC4F1 lines have been selected by back-crossing with 4 elite lines through a breeding program. Very recently, based on molecular analysis, we have selected another event, #10-2, which is also resistant to PepMoV. Horticultural difference was investigated for both GM lines, #T20 and 10-2, and no significance was found comparing to non-GM lines.

Alfalfa (Medicago sativa L.) is one of the most important forage crops in the world and it’s has been known as the best feed materials for dairy cows and other high valued animals. The new uses of alfalfa are being explored as bio-energy, food, medical and biochemical uses. R2R3-type MYB transcription factors play important roles in transcriptional regulation of anthocyanin biosynthesis. The R2R3-type IbMYB1 is known to be a key regulator of anthocyanin biosynthesis in the storage roots of sweetpotato. We previously showed that the expression of IbMYB1a led to anthocyanin pigmentation in tobacco and Arabidopsis. In this study, we generated transgenic alfalfa plants expressing the IbMYB1a gene under the control of CaMV 35S promoter. Overexpression of IbMYBa in transgenic alfalfa produced strong anthocyanin pigmentation in seedlings and generated a deep purple color in leaves, stems, roots, and even in seeds. High performance liquid chromatography (HPLC) analysis revealed that IbMYB1a expression led to the production of cyanidin as a major core molecule of anthocyanidins in alfalfa, as occurs in the purple leaves of sweetpotato (cv.Sinzami). We also examined expression of several structural genes in the anthocyanin biosynthetic pathway in alfalfa by RT-PCR analysis. In this presentation, we will further present molecular and biochemical characterization in IbMYB1a-overexpression lines. This result shows that the IbMYB1a transcription factor is sufficient to induce anthocyanin accumulation in the forage legume alfalfa plants.

Soybean self-sufficiency in Korea was 22.5% in year 2011, and as free trade agreement between Korea and US comes into effect, the amount of soybean importation increases. In 2012, paddy field soybean cultivation was over 0.1 million ha and it is expected to increase continuously due to rise in market price of soybean. Moreover, double cropping system including paddy field soybean is widely adopted nationwide, but studies on appropriate cultivar for this environment are insufficient. In this research, the effect of planting date and different cultivars on soybean growth and yield was investigated for three planting dates (June 20, July 5, and July 20) with 15 cultivars. According to ANOVA test, soybean yield was significantly different depending on sowing date and cultivars and interaction between sowing date and cultivars was also detected, meaning each of cultivars resulted different yield depending on sowing date. When planted on July 20, stem length, nod number, branch number and grain weight was decreased because of short growing period, resulting in yield decrease. Percentage of yield obtained from July 20 in contrast to that of June 20 showed that Pungwon, Nampung and Daepung recorded the least yield decrease and the highest yield when planted late. Evaluation of late-sowing-adaptable soybean cultivar in paddy field is now being carried on following last year.