국내 판매되고 있는 사슴벌레종[넓적사슴벌레(Dorcus titanus), 참넓적사슴벌레(Serrognathus consentaneus), 남방넓적사슴벌레(D. titanus jejuensis, 가칭), 애사슴벌레(D. rectus) 및 왕사슴벌레(D. hopei)]과 부산 지역에서 채집한 넓적사슴벌레 12개체와 경남 의령군에서 채집한 1개체의 미토콘드리아 DNA 중 cytochrome oxidase I(COI) 를 이용하여 계통을 비교분석하였다. 아울러 NCBI에 기등록된 북한, 일본, 대만, 필리핀, 인도네시아 및 태국의 넓적사슴벌레의 COI 서열을 이용하여 추가 분석하였다. 먼저 판매되고 있는 사슴벌레종은 넓적사슴벌레, 남방넓적사슴벌레 및 참넓적사슴벌레의 group I과 애사슴벌레 및 왕사슴벌레의 group II로 구분되었다. 부산지역 및 의령에서 채집한 넓적사슴벌레의 경우 COI 및 COII로 분석 결과 북한에서 기보고 된 3개체를 포함해 유전적 변이에 있어 큰 차이가 나타내지 않았다. 또한 한반도와 일본 일부지역에서 높은 상동성을 확인하였는데 그 외 아시아 지역에 서식하는 넓적사슴벌레와는 다른 그룹을 형성함을 확인하였다. 이러한 결과들은 국내 서식 사슴벌레 종의 유전적 분석과 함께 외래 유입종을 판별하는 근거자료로 활용될 수 있을 것이다.

We fabricated a Li-S battery with post-treated carbon nanotube (CNT) films which offered better support for sulfur, and investigated the effect of the surface properties and pore structure of the post-treated CNT films on Li-S battery performance. Post-treatments, i.e., acid treatment, unzip process and cetyltrimethylammonium bromide (CTAB) treatment, effectively modified the surface properties and pore structure of the CNT film. The modified pore structure impacted the ability of the CNT films to accommodate the catholyte, resulting in an increase in initial discharge capacity.

Conventional airport concrete pavement design uses empirical design method presented by FAA but it is not accurate because it does not consider environment load. In case of mechanistic - empirical design method used overseas, it needs to be modified according to domestic conditions. In this paper, a stress regression model considering environmental load and dual tandem gear load is developed as a mechanical - empirical design process and verified by fatigue model calibration. First of all, literature review was conducted on airports using DT gear as a design aircraft among domestic airports, and the physical properties of concrete pavement layers of each airport were identified. In addition, the environmental load is divided into the temperature load and the moisture load, and the temperature load is calculated from the climatic data of the region where the domestic airport is located, and the moisture load is calculated through the drying shrinkage prediction model developed through the previous study. The stresses occurring in concrete slabs under environmental loads and traffic loads were predicted using FEAFAA, which is a finite element analysis program, and factors predicted to affect concrete pavement were selected for the stress regression.As a result of the sensitivity analysis of the selected factors, the joint spacing, slab thickness, gear load, and road bearing capacity coefficient affected the stress behavior of the slab by more than 5% of the reference stress.According to the mechanical design, the finite element analysis was carried out for the consideration of only the traffic load and the environmental load and the traffic load based on the design factors. Based on the analysis results, multiple regression analysis was performed using the statistical analysis program SPSS and the stress regression equation was calculated. Then, the stress model for the environmental load and the traffic load was calculated and the final stress model with each stress model as the independent variable was derived. Using the calculated stress regression model, the stress was calculated and the bending strength of the concrete was divided to calculate the stress - to - strength ratio, and the appropriate fatigue model was selected and the correction was performed by the least squares method. This study was supported by Incheon International Airport Corporation(BEX00625)

The Acoptolabrus changeonleei Ishikawa et Kim, 1983 (Coleoptera: Carabidae), has been listed as an endangered insect in South Korea. The complete mitochondrial genome of the species was 16,831 bp with a typical set of genes (13 protein-coding genes [PCGs], 2 rRNA genes, and 22 tRNA genes) and one non-coding region, with the arrangement identical to that observed in most insect genomes. Phylogenetic analyses with concatenated sequences of the 13 PCGs and 2 rRNA genes, using the Bayesian inference (BI) and maximum-likelihood (ML) methods, placed A. changeonleei as a sister to the within-subfamilial species Damaster mirabilissimus in Carabinae, with the highest nodal support by both analyses.

As climate change and the increase of international trade, the possibility of infectious disease inflow is increasing into Republic of Korea (ROK). Nine of total 16 cases overseas travel related Zika virus disease and 140 of 314 cases in dengue fever occurred during mosquito season throughout ROK in 2016, which increase importance on the studies of disease vectors for effective control and monitoring. However, the vector resources for the studies are not enough to support the studies by private research institutes and universities. Korea Center for Disease Control and Prevention (KCDC) has secured and maintained the disease vectors, comprised of 5 genus and 15 species in an insectarium system. KCDC has supported the disease vector resources through the system wherever to request them. In this presentation, the insectarium systems will be introduced.

Mesenchymal stem cells (MSCs) are primary candidates for cell therapy and tissue engineering applications. A two-dimensional (2D) culture system is typically used for cell growth, but that method affects the characteristics of stem cells. The physiological cell environment connects cells not only to each other, but also to the extracellular matrix providing mechanical support, exposing the entire cell surface, and opening signaling pathways. The hanging drop method is the most widely used 3D culture method for spheroid formation. In this study, we investigated the relationship between spheroid size and changes in gene expression to determine the optimum spheroid size for use in tissue engineering. The expression levels of stemness factors such as NANOG, OCT4, and SOX2, angiogenic factors such as VEGF and IL-8, and osteogenic factors such as COX2 and TGF-β1 increased with spheroid size in the respective spheroid formation groups unlike the responses in their monolayer groups. Therefore, our results indicate that spheroid formation through the hanging drop method can increase the efficiency of MSCs-based tissue engineering over that obtained via traditional 2D cell culture systems.

Mesenchymal stem cells (MSCs) have been researched for use in biomedical applications, particularly for cell-based therapies and regenerative medicine due to their self-renewing capacity and ability to differentiate into multiple cell types such as adipose, bone, and tendon tissues. Cryopreservation of MSCs is a common preservation method that is advantageous for cellular therapies in human and veterinary medicine. Adipose tissue-derived cells have been shown to maintain their properties after cryopreservation. In this study, we investigated the morphology, proliferation (cumulative population doubling level and doubling time), cell surface markers (CD34, CD90, and CD105), and ability to differentiate into adipose, bone, and cartilage tissues in vitro of equine adipose tissue-derived MSCs (eAD-MSCs) and miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with and without long-term cryopreservation. The eAD-MSCs and mpAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 3 years and 2 years, respectively. Cryopreserved eAD-MSCs maintained their morphology, proliferation rate, and cell surface markers compared with fresh cells. With the exception of proliferation rate, cryopreserved mpAD-MSCs also maintained their fresh cell characteristics. The proliferation rate of cryopreserved mpAD-MSCs was higher than that for fresh cells. Cryopreservation did not change the adipogenic, chondrogenic, or osteogenic differentiation potentials of eAD-MSCs and mpAD-MSCs. In summary, long-term cryopreservation maintains the cell phenotype and differentiation ability of eAD-MSCs and mpAD-MSCs. These results might be useful when developing veterinary medicine and clinical applications.

The purpose of this study is to analyze the behavior of users of the Seomjin River cycling road, which is surrounded by gorgeous natural landscape. Further, we aim to understand the correlation between the importance and performance of various factors rated by users to obtain baseline data that can help to increase the usage of the bike path. We conduct a survey by performing one-on-one interviews at three locations, all of which have a certification center that verifies the completion of the bike path. The bike path attracts visitors from all over the country. First-time visitors and travel plans involving stays of at least one night account for a relatively high percentage of users. The primary purpose of the visit was more related to mental health issues rather than physical health issues. The most attractive feature of the Seomjin River is the beautiful landscape. However, the participation rate from clubs is low, and the visit rate to nearby tourist attractions is also low for scheduling reasons. Important factors that are considered using the bike path are accessibility, safety, bike path condition, amenity, and convenience, while the connectivity has a relatively low importance. We confirm that the convenience of the bike path is as important as providing a safe and amenity while surrounded by gorgeous landscape. The amenity and safety are rated highly for performance and while accessibility and convenience have low rating. The analysis of the detail evaluation items showed that the performance was high for surrounding gorgeous landscape, and non-crowded setting and performance was lowest for inadequate bike repair facilities. A comparison of the importance and performance showed that the performance lower than the importance, and had statistical significance. Accessibility, which is an external environmental factor, had the largest difference. On the other hand, for the detail evaluation items, the degree of crowding, surrounding landscape, and experience opportunities did not have statistical significance, but had a higher performance. This confirmed that they were factors that had the highest satisfaction for the Seomjin River cycling road. Importance-Performance Analysis (IPA) results indicated that the items requiring consistent maintenance were surrounding landscape, bike path signs, safety from pedestrians and vehicles, and convenient accessibility. Items that required specific improvement were convenient transportation for returning home, pavement material, and the degree of damage. On the other hand, items that were more effective in terms of their ability to maintain the current conditions were experiences of nearby rural villages, accessibility to nearby tourist attractions, local tourist attractions, and bike repair facilities. The bike repair facilities option had a considerably low performance compared to the importance. For items that were rated with low importance but high performance, it was more effective to allocate the effort for corresponding sections to other items. However, for the degree of crowding, which belonged to this particular category, it was actually more efficient to place on attracting users in order to increase the usage of the bike path.