A marine brown alga was collected from Geoje, located on the southern coast of Korea. This species shares the generic features of Dictyota and is morphologically characterized by the combined features of erect or prostrate thallus with smooth margin, dichotomous to subdichotomous branches not twisted at the terminal part, absence of surface proliferations, 1-3 cell layered cortex and unilayered medulla, and lack of involucrum in sporangia subtended by a single stalk cell. In a phylogenetic tree based on rbcL sequences, this species nests in the same clade with Dictyota bartayresiana. The genetic distance between both sequences within the clade was calculated as 1.2%, considered to be within the intra-species range for the genus. Based on these morphological and molecular analyses, this Korean alga is identified as D. bartayresiana described originally from the West Indies. This is the first record of D. bartayresiana in Korea.

A study was conducted to evaluate the effects of auxin, cytokinin, and their combined application to hasten their propagation using leaf cuttings. Different hormone levels were evaluated on two Echeveria species (E. subsessilis and E. runyonii). Three levels of auxin as represented by the use of indole-3-butyric acid (IBA) (0, 100, and 500 ppm), three cytokinin levels as represented by Kinetin (Ki) (0, 100, and 200 ppm) and their combination were applied and observed for 8 weeks. The use of 100 ppm IBA resulted in the highest shoot height, diameter, and rooting and shooting rate. Using 500 ppm IBA led the roots to develop the earliest but both species also had the highest mortality. The application of 100 ppm Ki significantly affected the majority of leaf cuttings of E. runyonnii which exhibited the highest and thickest shoots, and the number of leaves. The application of 100 ppm IBA and 100 ppm Ki is recommended to obtain increased shoot growth and development for leaf cuttings. In the case of single hormone use, the application of 100 ppm IBA may be preferable. The combination of auxin and cytokinin significantly stimulated the hastening of production of succulents using leaf cuttings.

Growth and development of succulents were studied in response to two leaf cutting types, the tip and base, using four Echeveria species (E. ‘A Grimm One’, E. ‘Momorato’, E. pulvinata ‘Frosty’, and E. pulidonis) and the planting position, either upright and faced-downward, using Graptoveria optalina. The shoot diameter (mm), height (mm), and number of leaves were significantly affected by the leaf cutting type. Results revealed that the shoot diameter of buds from base leaves were significantly larger by 4 - 9 times than those of buds from tip leaves. Shoot height of buds from tip leaves either not developed or reached a maximum of 2.29 mm while buds from base leaves had an average height of 11.61 mm from E. ‘Mamorato’. Using base leaves allowed to obtain roots and leaves for all selected succulent species after 60 days from planting. Regarding planting position, upright planted and downward-facing plants showed significant differences: buds from upright planting showed taller shoots, greater number of leaves which in turn gave higher visual quality rating and superior color reading using Hunter’s Cielab compared with buds in downward-facing planting position. Planting succulents in an upright position led to well-formed shoots and roots which had a high-quality rating and color evaluation compared with faced-downward planting producing etiolated and abnormal grown shoots. Based on these findings, we suggest that the use of base leaf cuttings and an upright planting position provides a rapid vegetative propagation method for selected succulent varieties.

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for detecting antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 5-h treatment of samples by placing them in water dosed with sulfadimethoxine (SDM; 200 g/ton water), the residue depletion of SDM was investigated in 25 cultured olive flounders (Paralichthys olivaceus). Muscles from fish were sampled before treatment and on the 1st, 2nd, 3rd, 4th and 5th days after treatment. The concentration of SDM in the muscle was then determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the residue in olive flounder muscle. To investigate the recovery rate, standard solutions were added to muscle samples to obtain final concentrations of 25 and 50 ng/mL in the muscle. The recovery rates of all spiked samples were >96.6% of the spiked value. SDM was detectable in the muscle of fish treated with the drug until the 1st day of the withdrawal period. The present study shows that the LFIA can be easily adopted to detect SDM residues in the tissue of farmed fish.

In the present study, to examine the effects of somatosensory stimulation of the hands on the hand functions and visuomotor coordination of children with developmental disabilities, a total of 24 sessions of experiments were conducted with three children with developmental disabilities for 60 minutes per session, three sessions per week from January 7, 2013 to March 11, 2013. To examine the effects of somatosensory stimulation of the hands on grasping and visuomotor coordination which are sub-areas of PDMS-2, the A-B study design was used which is a single case study method out of single-subject experimental research designs. Somatosensory stimulation of the hands was shown to be effective in improving the hand functions of children with developmental disabilities. Somatosensory stimulation of the hands showed positive effects on the raw scores and standardized scores of grasping and visuomotor coordination in all the three subject children. Visuomotor coordination functions showed higher scores in post-hoc tests in all three children. In particular, ring throws showed high scores at right 90°, right 45°, center, left 45°, and left 90°among visual directions. In the present study conducted with children with developmental disabilities, ring throws were performed to examine visuomotor coordination. In particular, the numbers of successes of ring throws on the front, left, and right were shown to be high. Therefore, somatosensory stimulation of the hands was effective on the hand functions and visuomotor coordination functions of children with developmental disabilities.

The fermented food Shindari is a low-alcohol drink that is indigenous to Jeju island, South Korea. In this study,the diversity of lactic acid bacteria (LAB) in Shindari was determined using a culture-dependent method. LAB were culti-vated from Shindari samples using two different LAB culture media. Twenty-seven strains were randomly selected and iden-tified by 16S rRNA gene sequence analysis. The identified LAB strains comprised 6 species within the Enterococcus,Lactobacillus and Pediococcus genera. Five of the species, namely Enterococcus faecium, Lactobacillus fermentum, L. plan-tarum, Pediococcus pentosaceus and P. acidilactici were isolated from MRS medium, while 1 species, L. pentosus, was iso-lated from Rogosa medium. Most of the isolated strains were identified as members of the genus Lactobacillus (78%). Thisstudy provides basic microbiological information on the diversity of LAB and provides insight into the ecological roles ofLAB in Shindari.

Nicotine, a major teratogen of cigarettes smoke induces embryonic abnormalities during the early stages of organogenesis. In this study, the protective effect of β-carotene against nicotine–induced embryos was evaluated by morphologic scoring, nile blue staining, lipid peroxidation, SOD activity assay and real-time PCR. The embryos exposed to nicotine (1 μM) revealed remarkable morphological anomalies compared to normal control group (p<0.05), but when β-carotene (1×10‒4 μM or 5×10‒4 μM) was added concurrently to the embryos exposed to nicotine, morphological parameters were significantly improved (p<0.05). Nicotine induced oxidative stress by increased lipid peroxidation, expression of proinflammatory cytokines (TNF-α and IL-1β), caspases-3 and decreased SOD activity. However, administration of β-carotene (1×10‒4 μM or 5×10‒4 μM) restored the SOD level and decreased oxidative damage in the embryos. These results indicate that β-carotene effectively counteracts the deleterious effects of nicotine on embryos and attenuates oxidative damage possibly through its antioxidant effects.

Nicotine, a major toxic component in tobacco smoke, leads to severe embryonic damages on organogenesis. We investigated if resveratrol can inhibit the nicotine–induced teratogenesis in the cultured mouse embryos (embryonic day 8.5) for 48 hours using a whole embryo culture system. The embryos exposed to nicotine (1 μM) revealed severe morphological anomalies, the increased levels of caspase-3 mRNA and lipid peroxidation, and further the lowered levels of mitochondrial manganese superoxide dismutase (SOD), cytosolic glutathione peroxidase (GPx), phospholipid hydroperoxide GPx, hypoxia-inducible factor 1α, and sirtuin mRNAs and SOD activity significantly compared to normal control group (p<0.05). However, whenre sveratrol(1×10‒5 μMor1 ×10‒4 μM) was added concurrently to the embryos exposed to nicotine, these all parameters were significantly improved (p<0.05).These findings indicate that resveratrol has a protective effect against nicotine-induced teratogenesis in mouse embryos throughout antioxidative and anti-apoptotic activities.

The purpose of this study was to identify the effects of manipulation on the velocity of cerebral blood flow and level of pain in cervicogeinc headache patients. The velocity of cerebral blood flow of 30 cervicogeinc headache patients(male=15, female=15, age=24.00±3.60) and 33 normal subjects(male=15, female=18, age=23.27±3.00) was compared. The 30 cervicogeinc headache patients were divided into suboccipitalis relaxation group, cervical manipulation group, and placebo group, and each were given different interventions. The velocity of cerebral blood flow and pain level was measured before intervention, and 1, 2, 3 weeks after intervention. The velocity of cerebral blood flow was measured with the Transcranial Doppler(TCD), and pain level was measured with visual analog scale(VAS). Blood flow velocity of middle cerebral artery in cervicogeinc headache patients was slower than those in healthy subjects. Physical therapy intervention did not have significant effect on velocity of cerebral blood flow, but slowly decreased at intervention for pain level increased. The suboccipitalis relaxation group and cervical manipulation group showed significant effect in decreasing pain level compared to the placebo group(p＜.05). Directly applied manipulation therapy in the neck area not only has effect on joint of cervical and soft tissue but also on blood vessels and nerves which pass the neck area, and because of those results of manual therapy seems to help recovery.

This study was carried out to investigate the properties of soil and water under Double-cropping SSH followed by whole crop barley (SSFWB) or by Hairy vetch (SSFHV) in paddy land. A field study was conducted at Baeksan-myeon, Kimje, Jeonbuk province in Korea on clay loam soil for 3 years. All plots were arranged in completely randomized design with three replicates. The pH and the contents of T-N and organic matter (OM) in soil samples collected at the end of the experiment were remarkably higher t㏊n those at the beginning of the experiment in both SSFWB and SSFHV treatment (P＜0.05). The contents of an exchangeable cations at the end of the experiment were remarkably higher t㏊n those at the beginning of the experiment in both SSFWB and SSFHV (P＜0.05). And then, the concentration of NO₃-N, NH₃-N and PO₄-P in discharge and leaching water were not influenced by the cropping system

This study was carried out to investigate the productivity and nutritive values of SSH under Double-cropping SSH followed by whole crop barley (SSFWB) or by Hairy vetch (SSFHV) in paddy land. A field study was conducted at Baeksan-myeon, Kimje, Jeonbuk province in Korea on clay loam soil for 3 years. All plots were arranged in completely randomized design with three replicates. The yield of SSH in SSFWB treatment was higher t㏊n t㏊t of SSFHV (P＜0.05). All nutrients values of SSH were not influenced by the cropping system. However, all nutrients of SSH in SSFWB treatment were just a little increase, compared with those of SSFHV.

This study are carried out to investigate the effects of double cropping system, such as winter whole crop barley followed by rice (WBFR) or after Sorghum × Sudan hybrid (WBASS), in paddy land on the properties of soil and water. A field study was conducted at Baeksan-myeon, Kimje, Jeonbuk province in Korea on clay loam soil for 3 years. All plots were arranged in completely randomized design with three replicates. The pH and the contents of T-N, P₂O? and organic matter (OM) in soil samples collected at the end of the experiment were remarkably higher t㏊n those at the beginning of the experiment (P＜0.05) in both WBFR and WBASS. The contents of an exchangeable cations (CEC) at the end of the experiment were remarkably higher t㏊n those at the beginning of the experiment (P＜0.05). The concentrations of NO₃-N, NH₃-N and PO₄-P in discharge and leaching water were not influenced by the cropping system.