The canine major histocompatibility complex (MHC) is referred to dog leukocyte antigens (DLA), which is known to be the most polymorphic genetic system in canine species. Many cloned dogs have been produced since Snuppy, first cloned dog, there was no research about genetic identity of MHC among cloned animals. Recently in Lee’s group, two non-transgenic cloned beagles (BG1, 2) were produced by somatic cell nuclear transfer (SCNT) using fetal fibroblast (BF). Also, four transgenic cloned beagles (Ruppy 1-3, 5) were generated using transgenic BF transfected with Red fluorescent protein (RFP) gene. We hypothesize that non-transgenic (BG1, 2) and transgenic (Ruppy 1-3, 5) cloned beagles derived from identical donor cells have the same immunological genetic characteristic except for RFP gene insertion in the genome. Thus, the aim of this study is to confirm the immunological identity of DLA class II in cloned beagles produced using same nuclear donor cell. Genomic DNA was extracted from blood of BG1, BG2, Ruppy 1, 2, 3 and 5. Genomic DNA of normal two control beagle, no correlation with BF was also investigated for rulling out the possibility that beagles were inbred. Forward and reverse primers used for DLA-DQA1 and DQB1 respectively were DQAF: 5’-TAAGGTTCTTTTCTCCCTCT-3’ and DQAR: 5’-GGACAGATTCAGTGAAGAGA-3’ DQBR:5’-CTCACTGGCCCGGCTGTCTC-3’ and DQBR: 5’-CACCTCGC CGCTGCAACGTG-3’. Polymerase Chain Reaction (PCR) products were purified, sequenced directly using the Big Dye Terminator kit. Sequencing analysis was performed on an automated 3730xl DNA analyzer. In experiment 1, sequence of DLA-DQ alpha 1 (DQA1) and DLA-DQ beta 1 (DQB1) exon 2, hypervariabel region, was compared in BG1 and BG2. Experiment 2 also compared the sequence of DQA1 and DQB1 among Ruppy 1, 2, 3 and 5. Experimental 3 compared sequence of DQA1 and DQB1 among all cloned dogs (BG1, BG2 and Ruppy 1-3, 5). As a result, BG1 and BG2 have same allele for DQA1 and DQB1 as we expected. They share DQA1*00101 and DQB1*02901 in experiment 1. In experiment 2, Ruppy 1, 2, 3 and 5 also have identical DQA1*00101 and DQB1*02901 allele. No discrimination between transgenic dogs and cloned dogs was seen in DQA1 and DQB1 Allele in experiment 3. DQA1, DQB1 allele was identified as *00101 and *02901 in all dogs. We provided the allele identity of DQA1and DQB1 in cloned beagles, which can be used as preliminary data for immunological related studies. In conclusion, transgenic cloned dogs despite of red fluorescent protein genes being inserted in their nuclear DNA were immunologically compatible with non-transgenic cloned dogs. We demonstrated that cloned beagles produced using identical nuclear donor were immunologically compatible.

This study was carried out to gain the basic informations about botanical characteristics of plant type and properties related to table quality for developing the new colored waxy corn hybrid. Materials used were produced by our laboratory in 2007 and their major traits were evaluated fro two years(2008 to 2009) at Corn Breeding Farm of Coll. of Agri. & Life in CNU. Items surveyed were major agricultural characteristics including ear length and traits related to edible. Botanical characteristics as a plant type and ear pattern of the developed waxy corn hybrids showed large ranges among them; stem height of CNU08H-h39 among used hybrids was the highest as 235.7cm, while CNUH08-15 was the lowest. Ear height of Daehackchal Gold 1 was the highest as 83.7cm and that of CNU08H-15 was the lowest among used hybrids. Ear length of CNU08H-h121 among used hybrids was the longest as 23.1cm, while that of CNU08H-71 was the smallest as 12.7cm. Kernel sugar content of the used hybrids appeared to be 16.1 to 13.0Brix(%). Especially, CNU08H-15, CNU08H-35 and CNU08H-h39 hybrids were highly appeared as 15 above. Pericarp thickness of check hybrid, Yeonongchal, was thicker as 46.0㎛, while that of CNU08H-39 compared to other hybrids showed the thinnest as 23.0㎛. From this study, we could obtain several superior colored hybrids with thinner pericarp and high sugar contents like CNU 08-39 and CNU 08-h121 hybrids. Accordingly, these hybrids will be apply as new developed variety through confirmed trials.

The aim of this study was to isolate chicken feather-degrading bacteria with high keratinolytic activity and to investigate cultural conditions affecting keratinolytic enzyme production by a selected isolate. A chicken feather-degrading bacterial strain CH3 was isolated from poultry wastes. Isolate CH3 degraded whole chicken feather completely within 3 days. On the basis of phenotypical and 16S rDNA studies, isolate CH3 was identified as Bacillus thuringiensis CH3. This strain is the first B. thuringiensis described as a feather degrader. The bacterium grew with an optimum at pH 8.0 and 37℃, where maximum keratinolytic activity was also observed. The composition of optimal medium for keratinolytic enzyme production was feather 0.1%, sucrose 0.7%, casein 0.3%, K2HPO4 0.03%, KH2PO4 0.04%, MgCl2 0.01% and NaCl 0.05%, respectively. The keratinolytic enzyme had a pH and temperature optima 9.0 and 45℃, respectively. The keratinolytic activity was inhibited ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and metal ions like Hg2+, Cu2+ and Zn2+. The enzyme activated by Fe2+, dithiothreitol and 2-mercaptoethanol.

‘Taegang’ is a new six-rowed covered barley cultivar developed by the National Institute of Crop Science (NICS), R.D.A. This cultivar is developed from a cross between ‘Suwon287’ and ‘Olbori’ in 1992. An F8 selection was made at NCES in 2000 and it was te

This study is to identify the physiological traits of submergence-tolerant varieties of rice plants (Oryza sativa L.) in Yeongnam area, southeastern part of Korea, where the reduction of rice yield due to submergence is remarkably severe. In the present study, two tolerant varieties of rice plants were selected from over 30 rice varieties grown in under a 10-day period. The tolerant varieties selected from a submerged paddy field. As a control, one intolerant variety of rice plant was chosen. Of the tolerant variety Samgangbyeo, rather than Haepyungbyeo, had a lower dissolved oxygen consumption and maintained a higher dry weight than the intolerant variety. The leaf photosynthetic rates (LPS) of the two tolerant varieties were significantly higher than that of the intolerant-variety after four days of submergence treatment. These results indicate that lower dissolved oxygen consumption in a limited pool is prevented by ethylene formation in the tolerant varieties, which may be a mechanism of submergence tolerance.