This study investigated the effects of drying methods and drying time on the changes in anthocyanin content in colored barley. Colored barley cultivar Boanchalbori was harvested at a time when the anthocyanin content was the most and dried in afield. The harvested barley was then treated by two methods, sun drying and shade drying, for 4, 8, 24, and 32 h. The moisture content of the sun-dried barley decreased slightly faster than shade-dried samples, but the difference was not statistically significant. Chemical analysis indicated that the samples dried under shaded conditions had slightly higher crude fiber and lower nitrogen free extract, but the difference was not statistically significant. There was no difference in the total digestible nutrients between the two methods. In the case of sun-dried barley, the anthocyanin content decreased compared to the control and shade-dried samples after drying for 4 h (p < 0.05), was maintained at a constant level at 24 h, and then decreased at 32 h. In case of shade-dried barley, the anthocyanin content decreased gradually with the drying time, and a significant decrease was found at 24 h of drying (p<0.05) as compared to the control. The shade-dried method was more successful in reducing anthocyanin loss than the sun-dried method (p<0.05). There was a slight decrease in 1,1-Diphenyl-2-Picrylhydrazyl radical scavenging with drying time in the shade-dried method, and a significant decrease after 4 h with the sun-dried method. These results showed that covering with a two-layer awning was advantageous to dry colored barley in the field conditions.

과학기술의 발달과 경제수준 향상 및 운동량의 부족으로 인한 비만증의 발생율이 높아지는 반면 체중 감량에 대한 관심도 증가하고 있다. 특히 이런 관심은 젊은 여성들 사이에서 발생하는 경향이 있으며, 지나친 체중감량은 건강에도 해를 미치지만 체중감량에 대한 자료가 거의 전무한 실정이다. 본 연구는 다이어트가 영양상태에 미치는 영향을 알아보고자 하여 제1보고로써 다이어트 실태에 대해 조사한 것이다. 1. 조사 대상자들은 19세(27%), 20세(25.3%)의 연령층이 가장 두터웠으며, 체중은 50.32±0.91kg, 신장은 160.90±0.47cm이었고, BMI는 18.73±4.89로 나타나 평균적으로 여윈 상태임을 보였다. 2. 조사 대상자들의 한달 용돈은 10∼15만원이 가장 많았으며, 부모님의 체형은 정상이거나 마른 분이 절반을 넘었다. 한편 아버지만 뚱뚱하신 경우보다는 어머니만 뚱뚱하신 분이 더 많은 것으로 나타났다. 3. 조사 대상자들의 식태도는 하루 한, 두끼만 섭취하며, 불규칙적으로 섭취하는 사람이 많았고, 과반수가 자신의 체형을 과대인식하는 것으로 나타나 체형의 기준을 바르게 선정할 수 있는 영양교육이 필요함을 보였다. 4. 조사 대상자들의 실제 체중은 50.32±0.91kg이었으나 이상체중은 46.94±0.39kg으로써 이상체중이 유의적으로 낮았다(p=0.0114). 5. 조사 대상자의 37.54%가 1997년 한 해 중 다이어트를 실시한 경험이 있었으며, 처음 다이어트를 실시한 시기는 고등학교, 대학생 때, 고등학교와 대학 사이의 순으로 나타나 주로 고등학교 이후에 다이어트를 하려는 이유로는 자기 신체에 대한 기대 및 느낌 등의 주관적인 이유가 주된 원인이었으며, 과반수 이상의 여대생이 다이어트를 실시해 본 경험(79.3%)을 가지고 있었고, 다이어트 실시 빈도는 가끔 실시하는 경우(38.25%)가 가장 많았다. 또한 다이어트의 실시기간은 일주일 미만인 사람이 가장 많았으며, 평균 체중감소량은 1∼2kg(37.69%), 2∼4kg(29.44%)이었으며, 과반수 이상이(76.65%) 감량된 체중을 유지하지 못하는 것으로 나타났다. 6. 가장 선호하는 다이어트 방법은 하루 한끼 혹은 그 이상을 굶는 방법, 운동, 하루 3끼를 모두 섭취하되 열량을 줄이는 방법 등의 순으로 나타나 결국 섭취열량을 제한하거나 소비열량을 늘이는 방법을 사용하는 것으로 나타났다. 7. 다이어트에 대한 정보는 주로 신문, 잡지(30.86%), 가족, 친구, 친척(28.86%) 등의 대중매체나 주위의 사람들로부터 얻는 것으로 나타났다. 8. 영양상태 지각도가 보통 이상인 경우는 아버지, 어머니 두 분 다 뚱뚱하지 않은 경우일 때는 116명(42.65%)이며, 두 분 다 뚱뚱한 경우는 8명(2.95%)로 나타나 부모가 정상체중이거나 마른 경우 그 자녀의 영양자각도가 그렇지 않은 부모의 자녀에 비해 높은 것을 알 수 있었다(p=0.004). 9. 영양상태 자각도는 자신의 체형을 적당하다, 약간 뚱뚱하다고 대답한 group이 다른 group에 비해 높았다(p=0.001).

양파를 생채로 착즙하여 분리한 액즙을 Brix 70%까지 감압농축시킨 것과 이어서 잔사에 ethyl alcohol을 가하여 추출한 것을 농축시켜 같이 합하고, 갈변방지를 위하여 1% cysteine의 첨가와 유화제로서 2% PGDR을 첨가하여 양파 oleoresin을 제조하고 저장중 품질 안정성을 검토하였다. 양파로부터 oleoresin에 추출된 당은 저장온도 5℃, 25℃ 및 40℃에서 매우 안정하여 저장 60일후까지도 함량변화는 거의 일어나지 않았다. 양파 oleoresin 추출 직후 갈변도를 나타내는 흡광도가 0.38로 담갈색을 띄었는데 5℃에서는 저장 60일 후에도 변화가 거의 없었으나, 25℃ 및 40℃에서 저장한 경우는 60일후 흡광도가 1.53 및 3.32로 증가하였고, cysteine을 1% 첨가한 경우는 대조구에 비하여 갈색 억제효과가 상당히 나타나 40℃에서 60일간 저장하였을 때의 흡광도는 대조구의 절반 수준으로 나타났다. 유화 안정성은 5℃ 저장의 경우 20일, 40일 및 60일후에 분리되지 않는 emulsion 층의 부피가 각각 96.8%, 94.1% 및 90.6%로 매우 안정하였고, 25℃ 및 40℃에서 60일 저장후는 83.2% 및 75.4%로 유화상태가 다소 불안정하였다. 생채 양파로부터 추출한 oleoresin을 저장시키지 않고 바로 대두유에 1% 첨가한 경우와 0.02% BHA를 첨가하였을 때 A.I. 는 각각 1.39 및 1.72를 나타내어 0.02% BHA를 첨가한 대두유의 유도기간 연장효과에 대하여 80.8%에 해당하는 항산화 활성을 나타내었고, 5℃ 및 25℃에서 60일 동안 저장한 양파 oleoresin을 대두유에 각각 1% 첨가하였을 때의 A.I. 는 1.37 및 1.30을 나타내어 실온 또는 그 이하의 온도에서는 양파 oleoresin이 가지는 항산화 활성은 매우 안정하였으며, 40℃에서 저장 60일후의 양파 oleoresin의 A.I. 는 1.08로 나타나 고온에서 저장기일이 길어질수록 양파 oleoresin의 항산화활성은 다소 불안정하였다. Overall odor intensity의 지표로서 total pyruvate 함량변화는 5℃, 25℃ 및 40℃에서 저장 60일후 잔존율이 각각 89.9%, 79.7% 및 65.2%로 저온에서는 상당히 안정하였다. 양파 oleoresin의 저장중 pyruvate 감소에 대한 반응속도 상수는 저장온도 범위 5∼10℃에서 1.381∼4.735mmol/ℓ·hr, Q10 값은 1.537∼1.694, 그리고 활성화 에너지는 11.649㎉/g mole이었다.

The aim of this study was to isolate chicken feather-degrading bacteria with high keratinolytic activity and to investigate cultural conditions affecting keratinolytic enzyme production by a selected isolate. A chicken feather-degrading bacterial strain CH3 was isolated from poultry wastes. Isolate CH3 degraded whole chicken feather completely within 3 days. On the basis of phenotypical and 16S rDNA studies, isolate CH3 was identified as Bacillus thuringiensis CH3. This strain is the first B. thuringiensis described as a feather degrader. The bacterium grew with an optimum at pH 8.0 and 37℃, where maximum keratinolytic activity was also observed. The composition of optimal medium for keratinolytic enzyme production was feather 0.1%, sucrose 0.7%, casein 0.3%, K2HPO4 0.03%, KH2PO4 0.04%, MgCl2 0.01% and NaCl 0.05%, respectively. The keratinolytic enzyme had a pH and temperature optima 9.0 and 45℃, respectively. The keratinolytic activity was inhibited ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and metal ions like Hg2+, Cu2+ and Zn2+. The enzyme activated by Fe2+, dithiothreitol and 2-mercaptoethanol.

Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates. The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2ㆍ6H2O 0.01%, respectively. The optimal temperature and initial pH was 30℃ and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was 113.8 ㎍/ml and 504.9 ㎍/ml, respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was 47.2 ㎍/ml and 334.0 ㎍/ml, respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.

The aim of this study was to isolate mesophilic chicken feather-degrading bacteria and to evaluate feather hydrolysate as alternative biofertilizer. Isolate RS7 was isolated from compost and identified as Bacillus pumilus according to API analysis and 16S rDNA sequencing analysis. Chicken feathers were completely degraded after 5 days of cultivation at 30℃. Feather hydrolysate treated by B. pumilius RS7 positively influenced Helianthus sannuus L. (sunflower) growth (e.g. growth rate, number and dry weight of leave, and flowering rate). These results suggest that feather hydrolysate prepared using B. pumilius RS7 could successfully be used as alternative biofertilizer, thereby reducing the environmental impact of feather waste from the poultry industry.

Pseudomonas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE 25μM within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can degrade TCE. Following a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least 250μM with no apparent retardation in rate, but the transformation capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation rate (Vmax) and saturation constant (Km) were 7nmo ℓ/min·㎎ cell protein and 11μM, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in 50·mℓ serum, vial that contained 10 ㎖ of meneral sals medium supplemented with 10μM TCE. TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.

This research was performed to investigate the dynamics of microbial community by RBC (Rotating Biological Contactor) using Rhodococcus sp. EL-GT and activated sludge. Cell counts revealed by DAPI were compared with culturable bacterial counts from nutrient agar. Colony counts on nutrient agar gave values 20∼25% and 1∼15% of cell counts (DAPI). The cell counts for the dynamics of bacterial community were determined by combination of in situ hybridization with fluorescently-labelled oligonucleotide probes and epifluorescence microscopy. Around 90∼80% of total cells visualized by DAPI were also detected by the bacteria probe EUB 338. For both reactors proteobacteria belonging to the gamma subclass were dominant in the first stage (1 and 2 stage) and proteobacteria belonging to the gamma subclass were dominant in the last stage (3 and 4 stage).

Several microorganisms which degrade phenol and trichloroethylene (TCE) were isolated from the activated sludge of a wastewater treatment plant. Among them, one isolate EL-04J showed the highest degradability and was identified as a Pseudomonas species according to morphological, cultural and biochemical properties. The phenol-induced cells of Pseudomonas EL-04J, which were preincubated in the mineral salts medium containing phenol as a sole carbon source, degraded 90% of 25μM TCE within 20 h. This strain could also utilize some of methylated phenol derivatives (o-cresol, m-cresol and p-cresol) as the sole source of carbon and energy. Cresol-induced cells of Pseudomonas EL-04J also cometabolized TCE.

Microorganisms utilizing petroleum as substrate were screened from the seawater in Pusan coastal area. Among them, fifty strains utilized bunker-A oil as a sole carbon and energy source. Five of these fifty strains were selected to experiment this study. According to the taxonomic characteristics of its morphological, cultural and biochemical properties, the selected strains were named Pseudomonas sp. EL-12, Flavobacterium sp. EL-15, Acinetobacter sp. EL-18, Enterobacter sp. EL-27 and Micrococcus sp. EL-43, respectively. The optimal medium compositions and cultural conditions for assimilation of bunker-A oil by the selected strains were 1.5-2% bunker-A oil, 0.1% NH4NO3, 1-1.5% MgSO4·7H2O, 0.05-0.15% KCl, 0.1-0.15% CaCl2·2H2O, 2.5-3.5% NaCl, initial pH 8-9, temperature 30℃ and aeration, respectively. The utilization and degradation characteristics on the various hydrocarbons by the selected strains were showed that bunker oil, n-alkane and branched alkane compounds were highly activity than cyclic alkane and aromatic hydrocarbon compounds.