본 연구는 울산항의 정박지 규모의 적정성을 평가하기 위한 방법을 제시하고, 이를 통해 현재 뿐만 아니라 항만개발에 따른 미래의 정박지 규모의 적정성을 분석하는데 그 목적이 있다. 이를 위해 울산항의 정박지 적정성 평가를 위한 정박지 가동률 개념을 제시하였다. 그리고 이 가동률 개념을 울산항의 2014년 정박지에 적용하여 가동률을 계산한 결과 모든 정박지에서 가동률이 100 %를 넘지 않는 것으로 도출되어, 추가 정박지 지정이 필요하지 않은 것으로 분석되었다. 또한 울산항의 2020년 가동률을 추정한 결과 E1정박지가 168.3 %로 가장 높았으며, E3정박지가 131.1 %, E2정박지가 118.5 %, 그리고 M정박지가 108.7 %인 것으로 계산되어, 2020년에는 정박지가 부족할 것으로 판단된다. 따라서 울산항의 항만개발에 따른 정박지 가동률을 100 % 수준으로 낮추기 위해서는 E1정박지는 11척, E2정박지는 1척, E3정박지는 2척, M정박지는 1척이 추가적으로 정박할 수 있는 수역이 필요할 것으로 분석되었다.

The market distribution channels has changed from single to multi-channels due to the diffusion of digital development products. In addition the new consumption emergence has been applied in showrooming and reverse-showrooming. One of well-known shopping district in South Korea, Dongdaemun, has been introduced Dongdaemun-based fashion brand which use on/offline multi-channel distribution. This study aims to segment multi-channel attributes of Dongdaemun-based fashion brands and analyse the effects of customer equity attributes, customer satisfaction, and re-purchase intention. The goals of this study are as follows: 1) to identify multi-channel attributes of Dongdaemun-based fashion brands, 2) to analyse the influence of multi-channel attributes on customer equity, customer satisfaction, and repurchase intention, 3) to explore the consumer group differences on shopping orientation. This study applied means-end chain theory to conduct in-depth interviews for the purpose of deriving the multi-channel attributes of Dongdaemun-based fashion brands and explore the influence of such attributes on customer equity, customer satisfaction, and re-purchase intention. This study collected 493 samples and structural equation modeling was used for final data analysis. The main findings of this study are as follows. The multi-channel attributes have a significant impact on value equity and brand equity. Relationship equity is influenced by product diversity, entertainment, and informativeness. The all of customer equity drivers have significantly impacts on repurchase intention and only brand equity have significant effect on repurchase intention. Also, customer satisfaction affects re-purchase intention. Brand equity have a significant influence on CLV. The consumers group differ in how consumers recognize multi-channel attributes of Dongdaemun-based fashion brands, based on channel preference. This study shows the importance of customer equity and suggests long-term implications for the development of Dongdaemun-based fashion brands.

Oyster mushroom is one of mushrooms that are cultivated and consumed a lot in Korea. P. ostreatus 'ASI 2504(Suhan)' is a preferable cultivar to mushroom farmers because it has a dark pileus and a thick stipe. But it is very sensitive to environmental conditions, so farmers demand an alternative cultivar of ‘Suhan’ continuously. To develop a new cultivar, parental strains ‘ASI 2504(Suhan)’ and ‘ASI 0665(Heuktari)’ were selected from P. ostreatus’s various collected strains according to morphological characteristics. P. ostreatus ‘Soltari’ was developed by the method of Di-Mono crossing between dikaryotic strain ‘Suhan’ and monokaryotic strain derived from ‘Heuktari’. Analysis of the mitochondrial genetic characteristics was performed for primary selection in 100 crossed strains. The mitochondrial DNA profile of ‘Soltari’ was same as that of ‘Heuktari’, when mitochondrial DNA primer MtPo1 was used. And a nuclear DNA profile of ‘Soltari’ was similar as those of the parental strains, ‘Suhan’ and ‘Heuktari’, when RAPD(Random Amplified Polymorphic DNA) primer URP 1, 3 were used. The optimum temperature for mycelial growth was 30°C for ‘Soltari’. ‘Soltari’ was appropriate for middle high temperature to grow, especially 13~18°C. Fruiting body production per bottle (1,100mL) was about 158.6g. When compared to the control strain ‘Suhan’, the stipe’s length and thickness of ‘Soltari’ were similar to those of ’Suhan’. But the pileus diameter of ‘Soltari’ was a little shorter than that of ‘Suhan’, the former was 42.72mm, while the latter was 51.33mm. And the pileus thickness of ‘Soltari’ and ‘Suhan’ were 18.18mm and 25.46mm, respectively. ‘Soltari’ was more resistant at high CO2 concentration than ‘Suhan’ and the color of pileus of ‘Soltari’ was kept dark gray at high temperature. Therefore, it is suggested that this new cultivar ‘Soltari’ be an alternative of ‘Suhan’ and contribute to energy saving effect in oyster mushroom farms.

본 연구에서는 국내 여수항만에 길이 500미터, 폭 200미터, 두께 2미터인 폰툰형 VLFS타입의 해상부두를 제안하였다. 이 구조물은 해상에서 오랫동안 파랑하중을 견뎌야하므로 파랑응답해석이 필수적이다. 유체-구조부 해석에는 직접법을 사용하였고, 연성운동방정식을 수치해석하여 응답 결과를 구하였다. 구조부는 유한요소법을 이용하여 계산하였으며, 유체부는 경계요소법을 사용하여 분석하였다. 탄성변형과 강체운동으로 인한 동적응답을 수치적으로 분석하였으며, 파장, 수심, 파향, 구조물의 강성 요소를 고려하여 규칙파에 대한 응답을 해석하였다. 연구의 결과, L/λ 1.5를 기준으로 응답이 변화하였고, 입사파의 방향에 따라 비틀림 현상이 나타났다. L/λ=8.0의 경우 수심이 증가할수록 입사측에서의 응답이 증가하는 경향이 나타났고, 강성의 변화에 따라 수직변위진폭의 피크점이 좌우로 이동하였다.

The button mushroom, Agaricus bisporus, is one of the major economical crops cultivated in Korea. This mushroom showed the 5th production to 11,493 M/T in 2014. Several fungus are known as the causal agents of diseases of the cultivated button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus). Cladobotryum mycophilum is the causal agent of cobweb disease of commercial mushrooms. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. A Gram-positive bacterium was isolated from mushroom media that markedly showed the antagonistic activity against Cladobotryum mycophilum, the most destructive pathogen of cultivated mushrooms. The HC57 strain was selected as antagonistic bacterium by inhibition zone method and it was identified as Bacillus subtilis by the cultural, morphological and physiological characteristics, and analysis of the 16S rDNA. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for Cladobotryum mycophilum cell, was sufficient for inhibition in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Cladobotryum mycophilum. Control efficacy of browning disease of strain HC57 treatment was 71% on Agaricus bisporus. The optimal culture medium for the antagonistic bacteria growth was determined as follows: 1.5% Xylose, 2% Soytone, 1% NH4H2PO4, 7 mmol CaCl2, and 0.5% Histidine at pH 6.0 at 25℃. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by Cladobotryum mycophilum.

This study was designed to evaluate the possibility of increase through dairy female offspring’s ratio by transfer of pre-selected transferrable blastocyst that was produced by pre-selected X-bearing semen with OPU derived oocytes. Elite dairy female cow is demanded strongly compared with male, the so called, farmer wants to produce only an elite female dairy offspring as a candidate female dairy cow for producing milk. In our study, we selected 2 elite dairy bull semen from National Agricultural Cooperative Federation to pre-select X-bearing semen and 5 elite dairy female cows as donor for collecting of OPU derived oocytes. OPU derived embryo production system was carried out an aspiration of immature oocytes from 5 donor cows 2 times per week, total 200 times for 2 to 7 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture. Dairy donor semen selected H-319, 320 bull in National Agricultural Cooperative federation was sorted X-bearing semen by flow-cytometer and frozen for using IVF with OPU derived oocytes. Donor cows were selected 5 elite dairy cows from Gyeongju Dairy Cow Community and then disease tests such as 4 kinds of disease before selecting was checked. Oocyte proportion of grade 1 to 3 from total collected oocytes was significantly lower in donor A and B than those in donor C, D and E (82.16 and 70.03% vs. 90.0, 91.78 and 93.57%), respectively (p<0.05). However, number of oocytes per session in donor A, C and E was significantly higher than those in donor B and D (7.77 ± 3.26, 5.85 ± 2.10 and 7.03 ± 2.14 vs. 4.68 ± 2.61 and 5.21 ± 1.97 oocytes), but donor A was significantly higher than donor C (p<0.05). Development to blastocyst in donor B, C and E was significantly higher than those in donor A and D (31.0, 25.0 and 25.0% vs. 14.3 and 4.5%), but donor A was not different in donor C and E (p<0.05). Nine out of 10 blastocysts (90.0%) derived from OPU blastocysts were confirmed male embryos that was induced with Y-bearing semen to confirm sex ratio only. Total 96 blastocysts derived from female bearing semen were transferred into synchronized recipients and then confirmed 42 recipients (43.8%) pregnancy rate, 36 offspring (37.5%) and 91.7% female sex ratio (33 female vs. 3 male offspring). Taken together all data, elite dairy female offspring could be produced effectively by in vitro production system between pre-selected x-bearing semen and OPU derived oocytes that would be influential breeder in the breeding of dairy farm to increase effectively elite dairy offspring ratio as well as net income in the dairy farmer.

A total of 25 strains of Flammulina velutipes were analyzed to identify the genomic regions responsible for producing white-fruiting body. NGS data was yielded by using Illumina Hiseq platform. Short reads were filtered by quality score and read length were mapped on the reference genome (KACC42780). Between the white- and brown fruitbody forming strains, we found 9376 SNPs, of which 8178 were non-polymorphic and 1198 were polymorphic. There is a high possibility that SNPs can be detected among the white strains as homozygous because white phenotype is recessive in F. velutipes. Thus, we constructed SNP matrix within 8 white strains. SNPs discovered between mono3 and mono19, the parental monokaryotic strains of 4120 strain (white), were excluded from the candidate. If the genotypes of SNPs detected between white and brown strains were identical with those in mono3 and mono19 strains, they were included in candidate as a priority. As a result, if more than 5 candidates SNPs were localized in single gene, we regarded as they are possibly related to the white color. In F. velutipes genome, chr08: 950kb-2650kb, chr09: 500kb-1400kb, chr09: 2800kb-4350kb, and chr11: 2450kb-3500kb regions were identified to be associated with white fruitbody forming.

This paper is conducted to support Korean game development companies to enter the MSNG market in China. According to preceding researches, the life-cycle of MSNG is under 3 months. Short-term of the MSNG's life-cycle raises the problems of low profits or deficit of companies managed to enter the Chinese market. Therefore, it needs to lead MSNG users to continuos use to extend the life-cycle. The plan to extend the life-cycle is likely to be a critical factor to be survived in Chinese market. The commitment is considered as the factor to make usage of MSNG longer by researchers. This paper also infers that the plan to keep MSNG user's commitment continuos develops their perceived functional value, emotional value and social value, with the reasonal, emotional and psychological point of view. We make a effort to reveal the relationships among factors through 318 data from Chinese MSNG users. Futhermore we would like to suggest that the companies consider continuos usage intention of MSNG users as the critical factor which makes a profit based on the result of this survey and propose the direction of future researches from the limit of this paper.