Panax ginseng C.A. Meyer, commonly known as Korean or Asian ginseng, is a perennial herb which is native to Korea and China. Its roots are highly prized for several medicinal properties. Therefore, Ginseng has been a top-ranked subject of many fields of scientific research worldwide. However, very limited number of research work has been published on species authentication using DNA marker system. In this study, 22 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 167 ginseng cultivars from 11 regions and 10 breed varieties. A total number of 111 alleles were detected, with an average of 5.05 per locus. The average expected heterozygosity and polymorphism information content (PIC) for SSR locus were 0.35 and 0.30, respectively. The model-based structure analysis revealed that 66.5% of all cultivars could be grouped into three populations with inferred value (allele shared >70%) membership. More than 33% of tested cultivars derived from two ancestries, which was basically consistent with clustering based on genetic distance. Almost all of the cultivars shared the ancestry with S1 and S2 except 1 China Jilin and 3 USA cultivars. The result indicated that most of Korean ginsengs are closely interrelated between the two ancestors but USA ginsengs are totally different from Asian cultivars.

Rice stripe disease, caused by rice stripe virus (RSV), is one of the major virus diseases in east Asia. The objective of this study was conducted to identify new resistance genetic source to rice stripe virus (RSV) disease. Genetic diversity of 155 rice cultivars was evaluated using 9 co-dominant InDel markers and STS marker ST10. These cultivars were classified into two groups by cluster analysis based on Nei`s genetic distances. The marker showed different band pattern among RSV resistance or susceptible cultivar. In comparison with bioassay for RSV resistance and genotyping using SSR markers showed that Stv-bi and InDel 7 marker observed recombination value within 3.8% and RSV resistance gene was closely related to InDel 7. Also InDel 7 divided as resistance type alleles and susceptible type alleles except for some varieties. Interestingly, 02428, Daw dam, Erguailai, Padi Adongdumarat, PERVOMAJSZKIJ, and Tung Ting Wan Hien 1 showed Japonica type in InDel 7 marker. However, these cultivars revealed resistant to RSV bioassay. These results indicate that those cultivar can be able to get the different gene resistance with Stv-bi gene. Newly identified resistance gene is considered useful for improving RSV resistance in japonica rice. Therefore, we will progress the allelism test and genetic analysis for identification of new gene source.

Glycolysis is responsible for the conversion of glucose into pyruvate and for supplying reducing power and several metabolites. Fructose-1,6-bisphosphate aldolase (AtFBA1), a central enzyme in the glycolysis pathway, was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant. Under high salinity conditions, aldolase activity and the concentration of NADH were compromised. However, expression of AtFBA1 maintained aldolase activity and the NADH level in yeast cells. AtFBA1 shares a high degree of sequence identity with known class I type aldolases, and its expression was negatively regulated by stress conditions including NaCl. The fusion protein GFP-AtFBA1 was localized in the cytosol of Arabidopsis protoplasts. The seed germination and root elongation of AtFBA1 knock-out plants exhibited sensitivity to ABA and salt stress. These results indicate that AtFBA1 expression and aldolase activity is important for stress tolerance in yeast and plants.

An Arabidopsis small GTPase, RabG3b, was previously characterized as a component of autophagy and as a positive regulator for xylem development in Arabidopsis. In this work, we assessed whether RabG3b modulates xylem-associated traits in poplar in a similar way as in Arabidopsis. We generated transgenic poplars (Populus alba x P. tremula var. glandulosa) overexpressing a constitutively active form of RabG3b (RabG3bCA) and performed arrange of morphological, histochemical, and molecular analyses to examine xylogenesis. RabG3bCA transgenic poplars showed increased stem growth due to enhanced xylem development. Autophagic structures were observed in differentiating xyelm cells undergoing programmed cell death (PCD) in wild type poplar, and were more abundant in RabG3bCA transgenic poplar plants and cultured cells. Xylogenic activation was also accompanied by the expression of secondary wall-, PCD-, and autophagy-related genes. Collectively, our results suggest that Arabidopsis RabG3b functions to regulate xylem growth through the activation of autophagy during wood formation in Populus, as does the same in Arabidopsis.

In order to improve rice dough functionality, we cloned 4 kinds of high-molecular-weight glutenin subunit (HMW-GS) genes from bread wheat, ‘Jokyeong’. Among them, we first examined Dx5 gene to generate marker-free transgenic rice for advanced quality processing of bread and noodles. The GluB1 promoter was inserted into binary vector for seed specific expression of the Dx5 gene. Two expression cassettes comprised of separate DNA fragments containing only the high-molecular-weight glutein subunit (HMW-GS) protein (Dx5) and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected to rice calli at 3: 1 ratio of Dx5 and HPTII, respectively. Then among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted both with Dx5 and HPTII gene to rice genome. We reconfirmed integration of the Dx5 and HPTII genes into the rice genome by Southern blot analysis. Wheat Dx5 transcriptsin rice seeds was examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only Dx5 gene were successfully screened at T1 generation. This result also provides that co-infection system with two expression cassettes could be efficient strategy to generate marker-free transgenic rice plants.

Allele mining in starch synthesis-related genes (SSRGs) has facilitated the discovery of desired natural sequence variations for eating quality in rice. This study investigated the sequence variations from 10 SSRGs, and further evaluated their relationship with the amylose content (AC) and rapid viscosity analysis profiles in a global collection of rice accessions by association mapping (AM). In total, 83 sequence variations were found in 10 sequenced amplicons, including 73 single nucleotide polymorphisms (SNPs), eight insertion-deletions (InDels) and two polymorphic simple sequence repeats (SSRs). Four subpopulations were identified by population structure analysis based on 170 genome-wide SSR genotypes. AM revealed 11 significant associations between three phenotypic indices and three sequence variations. One SNP with a g/c transversion at the 63rd nucleotide downstream of the OsBEIIb gene termination codon on rice chromosome 2 was significantly associated with multiple trait indices in both the general linear and mixed linear models (GLM and MLM), including the final viscosity (p < 0.001, R2 = 23.87%) in both 2009 and 2010, and AC (p < 0.01, R2 = 11.25%) and trough viscosity (p < 0.01, R2 = 20.43) in 2010. This study provides a new perspective of allele mining for breeding strategies based on marker-assisted selection.

We have identified ATTIRTA1 transposon, a kind of mariner-type DNA transposon from Brassica rapa genome. A total of 811 inverted-terminal repeat, ITR consisting of the both terminal on ATTIRTA1 transposon were found from B. rapa v1.1 sequence. Among them 616 ITR were paired by two in each transposon, indicating three quarters of the transposon exists in original form. Around 10 percentage of the transposon, 82 ITR was located in gene, expecially only in intron. Using these ATTRRTA1, we developed a display system modified from AFLP technique and applied for this system to analyze genetic diversity of Korea Brassica rapa core collection. The collection includes 220 accessions representing the different morphotypes and geographical origin. The analysis of population structure revealed five subgroups and the clustering patterns matched well with their morphological traits. ATTIRTA1 transposon display seems useful marker system for studying genetic relationships. Presently we have profiled the components and contents of glucosinolate in the core collection to analyze genome wide association. This collection will be helpful to identify agriculturally desirable traits from other supspecies.

“Joah”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA, Iksan, Korea, during the period from 1997 to 2011. The heading and maturing dates of this variety were April 26 and June 8 in upland, and April 28 and June 6 in paddy field, respectively. It is an awned, semi-dwarf and soft winter wheat. Culm and spike length of “Joah” were 78 cm and 8.4 cm. It had lower test weight (763g/L) and l,000-grain weight (43.0 g) than “Keumkang”. It showed moderate to pre-harvest sprouting, which lower rate of pre-harvest sprouting (12.3%) than “Keumkang”. “Joah” had lower flour yield (66.3%) and ash content (0.33%) than “Keumkang”. It showed higher lightness (92.29) of flour color than “Keumkang”. It showed lower protein content (11.5%) and gluten content (10.7%) and lower SDS-sedimentation volume(29㎖) and mixing time of mixograph than “Keumkang”. It was showed higher amylose content and high viscosity than “Keumkang”. It showed different compositions in HMW-GS compositions (2.2+12), PPO 18 (876bp) and Puroindolines composition compared to “Keumkang”. “Joah” showed similar firmness (2.20N) and higher volume of sponge cake to “Keumkang”. Average yield of “Joah” in the regional adaptation yield trial was 6.44 ton/ha in upland and 5.26 ton/ha in paddy field. “Joah” would be suitable for the area above -10℃ of daily minimum temperature in January in Korean peninsula.

A new rose variety, ‘Venus Berry’ was selected from the progenies of a cross between ‘Boy Friend’ and ‘GSR10315’ by rose breeding team of the Gyeonggi-Do Agricultural Research and Extension Services(GARES) in 2011. ‘Venus Berry’ was crossed in 2007 and seedlings were produced. After the test of specific characters from 2008 to 2011, it was finally selected and named. ‘Venus Berry’ was developed because of distinctive characters such as growth uniformity and high yielding potential. The petal of flower is so thick and has no scratch. A standard type with large sized flower, it has light pink(Red Purple Group 69C) color flower. ‘Venus Berry’ takes 45 days from pruning to blooming and cut flower productivity was 194.1 stems/m2 in a year. The length of cut flower was long with 65.5 cm. It has 10.2 cm in flower diameter and 43.6 in petal numbers per flower. Vase life of the this cultivar could be as long as 12 days.

A new rose variety, ‘Love Letter’ was selected from the progenies of a cross between ‘Red Giant’ and ‘Ensemble’ by rose breeding team of the Gyeonggi-Do Agricultural Research and Extension Services(GARES) in 2011. ‘Love Letter’ was crossed in 2007 and seedlings were produced. After the test of specific characters from 2008 to 2011, it was finally selected and named. ‘Love Letter’ was developed because of distinctive characters such as growth uniformity and high yielding potential. A standard type with large sized flower, It has red(Red Group 46A) color flower. ‘Love Letter’ takes 43 days from pruning to blooming and cut flower productivity was 152 stems/m2 in a year. The stems of cut flower have no thorn and the length was long with 70.5 cm. It has 9.3 cm in flower diameter and 32.4 in petal numbers per flower. Vase life of the this cultivar could be as long as 12 days.

“Joongmo2008”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It is an awned, semi-dwarf and hard winter wheat. The heading and maturing date of “Joongmo2008” were similar to “Keumkang”. Culm and spike length of “Joongmo2008” were 87 cm and 8.6 cm. It had similar test weight (800g/L) and higher 1,000-grain weight (46.7g) than “Keumkang”. It showed resistance to winter hardiness and moderate to pre-harvest sprouting, which lower rate of pre-harvest sprouting (9.8%) than “Keumkang”. “Joongmo2008” had higher flour yield (73.5%) and higher ash content (0.49%) than “Keumkang”. It showed similar lightness (90.01) of flour color than “Keumkang”. It showed higher protein content (16.4%), gluten content (13.4%), SDS-sedimentation volume(59㎖) and mixing time of mixograph than “Keumkang”. “Joongmo2008” had lower amylose contetn (24.5%) and higher viscosity (153BU) than “Keumkang”. It showed same compositions in HMW-GS compositions(5+10) and Puroindolines composition compared to “Keumkang”. “Joongmo2008” showed lower firmness (0.74N) of and higher bread volume of baked pan-bread to “Keumkang”. Average yield of “Joongmo2008” in the regional adaptation yield trial was 4.54 ton/ha in upland and 3.67 ton/ha in paddy field. “Joongmo2008” would be suitable for the area above -10℃ of daily minimum temperature in January in Korean peninsula.

‘Jungmo2502’ awnless barley line (Hordeum vulgare L.) developed at National Institute of Crop Science, RDA in 2011. The Jungmo2502 derived from three-way cross between Millyang100 and F1 (Samheung/Suwon300) in 2000. Subsequent generation were handled by the bulk method in a pedigree selection program. A promising line showed high yield as well as lodging resistance in the yield trial tested at Iksan in 2007 to 2008, designated as Iksan 448. The line in the regional yield trials(RYT) tested in seven locations around Korea for three years from 2009 to 2011. The Jungmo2502 is carrying the growth habit of Ⅳ, green leaf and stem similar to the check cultivar ′Youngyang′. Its heading date was May 3, and maturing time was May 30 in paddy field, which was 3 days later respectively than check cultivar. It was 98 cm of culm length, 607 spikes per m2 and showed more rate of leaf blade, winter hardiness, and resistance to BaYMV than the check cultivar. Average dry matter yield was similar with the check cultivar in paddy field (about 11 ton ha-1). It also showed good qualities as 8.7% of crude protein, 21.9% of ADF (acid detergent fiber), 40.7% of NDF (neutral detergent fiber), and 71.6% of TDN (total digestible nutrients), including high grade of silage for whole crop barley.

Pollen development in flowering plants is regulated by a comprehensive pattern of genes. One way to produce hybrid rice based on nuclear male sterility is to find out firstly the potential promoters that function specifically in anthers since it is a specific site for transcription initiation and play key roles for the spatial and temporal expression of the genes. To implement this objective, we were selected promoter region of 16 genes based on the expression pattern of microarray and then those were introduced into the promoterless final destination vector which containing the GFP and GUS reporters genes. The resulting twelve vectors were transformed into monocotyledonous rice (Oryza sativa L) and a dicotyledonous Arabidopsis as heterologous system. Minimum 20 plants for each vector were analyzed by histochemical GUS assay at the flowering stage in Arabidopsis. 9 vectors out of 12 vectors constructed were expressed exclusively at the anther, especially in pollen, however one vector exhibited expression in stigma. For rice, T-DNA insertion were confirmed with specific primers in each promoter and GFP region. All T0 transgenic plants contained T-DNA insertion in their genome. This study would provide valuable information for biotechnological application for the induction of male sterility in plants.

광나무(L. lucidum)는 ursolic acid와 oleanolic acid를 다량 포함하고 있다. 본 연구에서는 광나무 열매, 줄기, 잎 세 부위 추출물의 항주름 효능을 평가하였다. 광나무 추출물은 human skin fibroblasts에서 독성이 없을 뿐만 아니라 MMP-1과 MMP-2의 발현을 감소시키고 COL1A1의 발현을 증가시켰다. 이들 추출물은 모두 농도 의존적으로 COL1A1의 발현을 증가시켰으며 MMP-1과 MMP-2의 발현을 감소시켰다. 광나무 세 부위 추출물 가운데, 열매 부위에 가장 많은 양의 ursolic acid 와 oleanolic acid가 함유되어 있었으며 가장 강한 COL1A1 upregulating 효과와 MMP-1과 MMP-2 downregulating 효과를 나타냈다. 이처럼 항주름 효능을 보이는 광나무 열매 추출물은 기능성 화장품 소재로 개발될 수 있는 가능성이 있다.

The potential cultivation of buckwheat species in Luang Prabang region, Lao PDR where the species has not been naturally grown and not traditionally utilized was studied to introduce buckwheat as a new agricultural material, especially for food and medicine. The growth of Tartary buckwheat species was higher than common buckwheat showing 2.3 fold of plant height compared to common buckwheat. Number of flower per plant in Tartary buckwheat (731) was three fold higher than common buckwheat (244). Dry weight per plant was respectively 1.7 g in Suwon No. 1, 2.6 g in Suwon No. 2, and 1.4 g in KW45. The rate average of seed setting was similar between common buckwheat (41 to 46 %) and Tartary buckwheat (44%). Total seed yield of Tartary buckwheat (184 kg/10 a) in 10a could be estimated to be higher than that of common buckwheat (108 kg/10 a).

This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at 25℃ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.

Identification of the modal properties of a structural system has received much attention over the years because of its importance in structural model updating, structural health monitoring and structural control. This paper presents experimental modal test results such as natural frequencies and mode shapes of a scale model of floating structure. A modal testing is performed on the structure and modal parameters for the structure are extracted from the measured data. The results are compared to a finite element model and the correlation between the measured and analytical modal parameters is investigated.

This study was conducted to compare the protein characteristics, dough rheology and bread loaf volume of Korean wheat cultivars and CIMMYT lines produced in diverse environments and to determine the genetic and environmental effects on bread making quality. Protein characteristics, including protein content and SDS-sedimentation volume, mixing properties during dough development and bread loaf volume were primarily influenced by the environment. Wheat cultivated in Jinju exhibited higher SDS-sedimentation volume based on constant protein weight and bread loaf volume than those in Suwon and Iksan. SDS-sedimentation volume based on constant protein weight, mixing time of mixograph and mixing tolerance of mixograph were positively correlated with bread volume. Korean wheat cultivars showed different allelic variations of Glu-1 and Glu-3 compared to CIMMYT wheat lines. Alchanmil, Keumkangmil and Tapdongmil could be suitable for bread making because these cultivars exhibited a 10 point Glu-1 score. However, Korean wheat cultivars should be introduced specific alleles in Glu-3 loci, including Glu-A3b or d and Glu-B3b , d , f or g , to improve gluten strength related to increase bread loaf volume.