Porous carbon from charcoal filled polypropylene composites were prepared and their mechanical properties were evaluated. In preparing the composites, crosslinking agent (sodium benzonate) were used in order to improve the bonding force between matrix and fillers. In this study, the effects of charcoal powder and sodium benzonate concentration on the mechanical properties and interface phenomena on the composites were evaluated. The mechanical properties of composites increased progressively with the decrease of filler loading. In the case of addition of the crosslinking agent into the composite, the mechanical properties were increased and showed maximum value at the 3 wt% concentration of sodium benzonate. According to the result of the TGA, the weight loss of composite according to crosslinking agent was not observed and initial thermal degradation temperature of composite reinforced charcoal was located at 390℃.

Purpose - In this study, the safety related tasks for low cost carriers’ were examined and measures to improve them were suggested. As the air demand increases rapidly, number of passenger has mushroomed in Korea and in order to keep with the demand low cost carriers have played a role. However, low cost carriers’ safety related tasks are not reliable level so far. Thus, in this study, the low cost carriers’ current situations in terms of safety are investigated, especially regarding how well the ICAO’s new safety policies are applied. Research design, data, and methodology - The results of the Ministry of Land, Infrastructure and Transportation’ investigation are introduced and field study results are analysed. Current status of retaining experts, staffs, administrators in terms of aviation safety tasks of low cost carriers is compared to those of major airliner and low cost carriers’ aviation safety culture is also analyzed by examining the systems and questioning staffs. Especially the culture regarding aviation safety is very important because the culture surrounding the safety tasks plays a major role in every respect of conducting the tasks. Results – Overall the current status of low cost carriers’ aviation safety tasks is below standard. Especially retaining experts and staffs is basically below the required level. Also, system and organization to conduct safety tasks are not satisfactory. In particular, aviation safety culture is not settled to operate appropriately. Conclusions - The International Civil Aviation Organization (ICAO) and member states are continuing to launch new safety policies in response to the surging demand for air travel worldwide. The most urgent and important issue among the various safety policies is to reduce air accidents. In order to reduce the number of accidents, ICAO decided to reduce the number of accidents by using the Reactive Safety Management. ICAO has to ensure that each member country can implement proactive safety management for aviation safety. A safety management system (Safety Management System) is a system in which each member state implements a fulfillment standard. The current situation and problems of the safety management system for each airline are suggested and proposed for improvements.

본 연구에서는 미계측 도시유역의 수공구조물 설계기준의 불확실성을 검토하기 위해 과거관측자료(S0)를 기준으로 상세화 기법(downscaling) 및 편의보정(bias correlation)을 통해 생산된 RCP 4.5 기후시나리오 HadGEM3-RA (RCM)모델을 이용하여 S1 (2017~2046년), S2 (2047~2076년), S3 (2077~2100년) 기간의 확률강우량의 변화를 평가하고, 도시유출모형을 이용하여 최대첨두홍수량을 산정하고 기후변화 기간별 영향을 분석하였다. 이때 확률분포형은 Gumbel, 매개변수 추정은 최우도법(ML)을 사용하였다. 평가 결과 대부분의 도시배수시설물 설계빈도인 10년 빈도의 경우 3사분위값을 기준으로 50년 미래를 가정할 경우에는 약 10%, 70년 이상의 미래를 가정할 경우에는 약 20%의 확률 홍수량 증가가 예상되었다. 이러한 결과는 현재를 기준으로 설정된 설계홍수량으로 설치된 도시배수시설물이 미래에는 설계기준에 미달하는 시설물이 될 수 있다는 것을 의미하며, 기후변화에 대응 위해서 설계기준에 시설물의 내구연한을 고려한 미래 기후상태를 반영해야할 것으로 판단된다.

Dual-color fluorescence in situ hybridization karyotype analysis was created using repetitive sequences including two types of rDNA repeats (45S and 5S rDNAs) and Arabidopsis-type telomere sequence repeats. The somatic metaphase cells of Carthamus tinctorius were observed as diploids (2n=2x=24). A symmetrical or slightly asymmetrical karyotype with seven pairs of metacentric and five pairs of submetacentric chromosomes was observed. The lengths of the somatic metaphase chromosomes ranged from 4.18 to 6.53 ㎛, with a total length of 60.71 ㎛. One locus of 45S rDNA was located on the pericentromeric regions of three pairs of chromosomes and the other pair was situated on the terminal regions of the short arms of a single pair of chromosomes. One locus of 5S rDNA was detected on the interstitial regions of the short arms of two pairs of chromosomes. Arabidopsis-type telomeric repeats were detected on the terminal regions of all pairs of chromosomes. Co-localization of loci between telomeric repeats and 45S rDNA was observed in a single pair of chromosomes. The results provide additional information for the existing physical mapping project of C. tinctorius and will also serve as a benchmark to a more intricate cytogenetic investigation of C. tinctorius and its related species.

One of the reasons to causing blood coagulation in the tissue of xenografted organs was known to incompatibility of the blood coagulation and anti-coagulation regulatory system between TG pigs and primates. Thus, overexpression of human CD73 (hCD73) in the pig endothelial cells is considered as a method to reduce coagulopathy after pig-to-non-humanprimate xenotransplantation. This study was performed to produce and breed transgenic pigs expressing hCD73 for the studies immune rejection responses and could provide a successful application of xenotransplantation. The transgenic cells were constructed an hCD73 expression vector under control porcine Icam2 promoter (pIcam2-hCD73) and established donor cell lines expressing hCD73. The numbers of transferred reconstructed embryos were 127 ± 18.9. The pregnancy and delivery rate of surrogates were 8/18 (44%) and 3/18 (16%). The total number of delivered cloned pigs were 10 (2 alive, 7 mummy, and 1 died after birth). Among them, three live hCD73-pigs were successfully delivered by Caesarean section, but one was dead after birth. The two hCD73 TG cloned pigs had normal reproductive ability. They mated with wild type (WT) MGH (Massachusetts General Hospital) female sows and produced totally 16 piglets. Among them, 5 piglets were identified as hCD73 TG pigs. In conclusion, we successfully generated the hCD73 transgenic cloned pigs and produced their litters by natural mating. It can be possible to use a mate for the production of multiple transgenic pigs such as α-1,3-galactosyltransferase knock-out /hCD46 for xenotransplantation.

Purpose – This study explores the role of construal levels in predicting online consumer behavior on a retail website. It builds on the conceptualization that simply browsing a website and making actual purchases can be an outcome of how abstractly or concretely one thinks about that experience. This study examines the differential effects of intermediary websites’ attributes and seller’s product offerings in predicting frequency of visits and actual purchases. Research design, data, and methodology – Data were collected from 188 undergraduate students in a large university of Korea. Hierarchical regression model was utilized to test the proposed effect of website characteristics and seller attributes on visit and purchase. Results – We propose and find that online shopping website visits and purchase frequency have different antecedents. The results reveal that website visit frequency and purchase have different predictors and this can be explained through construal level theory. Specifically, we find purchase frequency is predicted more by website image and financial benefits can be more predictive in actual purchases. Conclusions – Consumer behavior on the internet can be delineated into website visits and actual purchases. First, uplifting the image of the website itself is much more important than just making offerings cheaper. Online shopping website should try to match its features to mental representations that customers go through from just visit (abstract) to purchase (concrete).

Endocrine disruptors are exogenous chemicals that their endocrine disrupting effects mediated by androgenic signaling plays crucial roles in the control of development and several androgen-related diseases. However, there are no authorized in vitro screening and testing methods to evaluation of (anti-)androgenic activity. To find out a better in vitro cell line model, we have previously reported that 22Rv1 cells, a human prostate cancer cells contained functional Androgen Receptor (AR), might be an appropriate model for the evaluation of (anti-)androgenic endocrine disruptors. Based on this result, we developed a stable 22Rv1/mouse mammary tumor virus (MMTV) cell line to test AR-mediated transcriptional activation (TA). Using 22Rv1/MMTV cells, we established the test protocol and optimized the testing condition for AR-TA assay. In this study, we performed the inter-validation assay by four different laboratories to evaluate the 20 coded chemicals which were selected from the ICCVAM list (ICCVAM, 2003) or academic articles that exhibited exact (anti-) androgenic activity. The statistical analysis of the results of the inter-laboratory validation study revealed that there was reproducibility between the four participating laboratories. In conclusion, 22Rv1/MMTV AR-TA assay might be a quick and relatively inexpensive method, which can be used to screen large numbers of chemicals for their potential to activate or inhibit AR-mediated gene transcription. Furthermore, it will provide mechanistic data relevant to understanding adverse reactions observed in intact organisms.

In the genus Chrysanthemum, repetitive DNA sequences, the dominant part of a genome, are still to be elucidated. To explore the matter, the present study applied fluorescent in situ hybridization (FISH) to the mitotic metaphase chromosome of Chrysanthemum boreale with C0t DNA as probes. Based on DNA re-assotiation kinetics, three kinds of C0t DNA exhibiting different degrees of repetitive nature were fractionated and used as FISH probes to map the repetitive sequences. Signals from all C0t DNAs were successfully observed but their coverage on the chromosomes was different among C0t-1, C0t-10, and C0t-100. C0t-1 FISH signals resulted to have its intensity on the telomeric region and were also dispersed on both chromosome arms except for some distal regions. In C0t-10, signals were observed in all parts of the chromosome with greater intensity around pericentromeric regions. FISH with C0t-100 DNA was observed in bright signals all over the chromosome. Signals of C0t FISH found in this study covered the regions where ribosomal DNAs and telomeric repeats of C. boreale have been distributed (previous report), thus signifying their repetitive attributes. The present results could enhance the efficiency of studying genomes, chromosomes and repetitive sequences of C. boreale and subsequently hasten the realization of the genetic scheme of Chrysanthemum.

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 μM roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were 98 ± 35.2 and 145 ± 11.2, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

This study investigated the effect of NH4 + concentrations on microalgae growth by appling mixotrophic microalgae chlorella vulgaris in order to treat anaerobic digested food waste leachate. The growth rate and final microalgae growth were an order as 400 > 100 > 800 > 1300 mg-N/L. As results, The growth rate and final growth of microalgae were highest at ammonia concentration of 400 mg-N/L, On the other hand microalgae growth was inhibited when ammonia concentrations were over 800 mg-N/L. high concentrations of nitrogen over 800 mg-N/L interrupt the growth of microalgae. All of nitrogen and phosphorus were removed by microalgae at the ammonia concentration of 100~400 mg-N/L. In addition, when ammonia concentration was over 800 mg-N/L, the removal of nitrogen and phosphorus was limited mainly due to the microalgae growth limit. It was possible to treat anaerobic digested food waste leachate with mixotrophic microalgae when the ammonia concentration was controlled below 400 mg-N/L.

There is about 80% organic acid in the Food wastes leachate and organic acid can be used as organic carbon source for mixotrophic microalgae. The mixotrophic cultivation is that the combination of autotrophic and heterotrophic cultivation, where inorganic and organic carbon sources are used in their methabolism simultaneously. Therefore, this study investigated the effect of various organic acid on the microalgae when Food wastes leachate treatment. Chlorella vulgaris was used in this study, also NaHCO3 is supplied at all conditions as inorganic carbon source. In order to see the effect of organic carbon sources centrally, the carbon source conditions was set in 5 conditions - glucose known to have excellent effectiveness to increase of biomass productivity 1500 mg-COD/L, acetate 1500 mg-COD/L, isobutyrate 1500 mg-COD/L, propionate 1500mg-COD/L and the mixed organic acid (acetate, isobutyrate, propionate each 500 mg-COD/L). This study evaluated final biomass production, consumption of organic carbon, and N,P removal. In the result, the final biomass productivity by using glucose, acetate and isobutyrate is the highest level, followed by mixed organic acid and propionate. This is same aspect in N,P removal; the more rapid growth rate is, the more rapid N,P removal rate is. In the view of consumption of organic carbon, acetate make best use among organic acid.