To assess the temperature change effects on Collembola communities, we selected 9 Korean mountains in different latitudes. Top soil was collected and Collembola extracted from 3 sites in different elevation in each mountain. Extracted Collembola were sorted to genera and this data were summarized as total abundance, family richness and diversity. Additionally, soil temperature in each plot, physical and chemical properties of the soil was investigated and that correlated with Collembola community data. Average soil temperature and soil properties affected patterns in Collembola abundance and family richness. The results indicate that temperature change and soil properties strongly affected Collembola communities. Therefore, Climate change has high potential to affect Collembola communities.

Estimating compensation payment for environmental damages by chemical accidents has been controversial because accurate quantification of damages of chemical accident is so complicated and there is lack of scientific studies about impact of chemical accidents. In this study, for supporting chemical accidents compensation criteria, the 7 days acute and 28 days chronic toxicity test was conducted with 4 major chemical accident substances (Sulfuric acid, Nitric acid, Methanol and Methylethylketone) according to the OECD 232 guidelines. Methylethylketone was most toxic chemical followed by methanol, nitric acid and surfuric acid. Further study of comparison between pH and chemical effects was needed.

Berothidae is one of the uncommon group of Neuroptera. The family currently include about 22 genera and 100 species in the world. The genus Isoscelipteron include about 20 species in the world, with the distribution covering Eurasia. Several species of this family are known to be a termite predator during larval stage. We collected Isoscelipteron pectinatum adult, which is a new species record in Korea, which is in fact a new family record as well. Here we provide a brief description, photos of adult and male genitalia, and DNA barcode sequence based the specimen examined.

This study was conducted to evaluate a visual surveillance system. The advancement of recording technology and network service make it easy to record and transfer the videos. Moreover, progressed recognition technology help to make a distinction each other. Cows show distinguishing behaviors during their estrus period. The mounting is one of the behaviors. The result was different depending on the breed of the cows and the size of the farm. In the case of Korean native cattle, the estrus detection rate was 71.15%, however, dairy cows, the estrus detection rate was 39.38%. At the farms having below 6 modules, the estrus detection rate was 87.41%. On the other hand, at the farms having over 6 modules, the estrus detection rate was 77.78%. With the proper progress, the visual surveillance system can be used to detect heat detection.

The previous social welfare system medical treatment, economy, society, etc, failed to guarantee “quality of life,” in various respects. In such a situation, green areas can be devised as an alternative means of directly improving the quality of life. Furthermore, natural resources and ecosystem services are essential elements of environmental welfare that have the purpose of supporting health and quality of life. However, despite the importance of such green space, it is often unavailable and there is a great disparity in its distribution among different regions in urbanized areas where residents are intensively dispersed. Therefore, the present study suggests a plan for reducing the regional imbalances in the green environment by using distributive equality and visual access as indexes, and Dalseo-gu, Daegu Metropolitan City as the subject; the research results are deemed to be sufficient for carrying out the important role of providing the basic data necessary for establishing future environmental welfare policy. An examination of the analysis results are as follows. First, Gamsam-dong was revealed to need additional green areas; out of the 167 spaces that were regarded as possible for green biotope construction, 48 spaces appeared possible for additional green biotope construction. However, only 10 spaces were assessed as having the potential for actual green biotope construction, excluding the spaces affected by local green environments. Moreover, given a concern with green equality, a final two were deducted as additional green spaces. The present study is significant in that it accomplishes visual accessibility analysis in terms of visual quality, including the effects of aesthetic enjoyment along with distributive equality from green biotopes; the relevant subfactors include green area supply and demand when constructing additional green biotopes to realize a city with improved environmental welfare. However, the present study is limited in terms of failing to consider the green biotope of local administrative districts affecting the target area of the study because it analyzes equality and disconnected green areas for selecting additional green biotope spaces using Dalseo-gu Daegu as its subject.

백색, 적색(680±20nm), 황색(600 ± 20 nm), 녹색(525 ± 25 nm),청색(400 ± 10 nm), 흑색(365 ± 10 nm) 및 변환(다양한 파장)램프를 가지는 솔라트랩을 잡곡 및 두류포장에서 설치하여 나비목, 노린재목 및 딱정벌레목의 주요 해충에 대한 유인효과를 잡곡 및 두류포장에 설치하여 10일 간격으로 조사하여 평가하였다. 채집된 충수는 조사시기와 램프의 색깔에 따라 크게 영향을 받았으며, 조사시기와 램프의 색깔의 상호작용 효과도 있었다. 암청색램프에서 매미충류를 제외하고 가장 많은해충이 유인되었다. 따라서 암청색램프는 해충의 대량포획뿐만아니라 예찰을 위해 효과적으로 사용될 수 있다. 솔라트랩의 암청색램프와 페로몬의 조합은 잡곡 및 두류 해충의 포획효과를 증진시킬 수 있어 종합관리를 위한 유망한 수단으로사용될 수 있을 것으로 여겨진다.

Toxicity test of contaminate soil is very complex because of differential bioavailability in the soil. Therefore, bioavailability of metals in soil is a major factor influencing estimates of toxicity. In this study, the two major test was conducted. First, the toxicity of arsenic for the Collembola, Paronychiurus kimi, was assessed by determining the effects of increasing arsenic concentration on survival, reproduction and body concentration of As in five forested soils with different available phosphate and oxide-metal concentration. Second, the sequential extraction procedure (SEP) for arsenic by choosing extraction reagents commonly used for sequential extraction of metals was tested. The EC50 based on total As concentration in soil was estimated respectively. The available phosphate and oxide-metal concentration in soil influenced on As fraction in soil. Especially, As in soil which is non specifically and specifically sorbed (fraction 1, 2) has strong correlation with available phosphate and oxide-metal concentration (p<0.05). The toxicity is more higher in the soil with high available phosphate and low oxide-metal concentration. In addition, the high arsenic concentration in fraction which is amorphous and poor-crystalline hydrous oxide of Fe and Al (fraction 3) had effect to the toxicity. As a result, the toxicity of As is related with As concentration in fraction 1, 2 and 3 and the soil properties and the arsenic fractionation in soil have a influence on the bioavailability and toxicity.

Thc climate change has the potential to significantly modify the actual distribution of insect pest with unknown consequences on agricultural systems and management strategies. In this study, Thrips palmi Karny was selected to predict distribution under climate change. T. palmi was introduced and first recorded in 1993 in Korea, and has become a serious pest of vegetable and ornamental crops. The MaxEnt was applied to T. palmi to predict its potential geographic distribution in Korea and Japan under the RCP 8.5 climate changing scenario. The MaxEnt software package is one of the most popular tools for species distribution and environmental niche modeling. The habitat prediction model of T. palmi in Korea was validated by the distribution of T. palmi in Japan. Based on the MaxEnt modeling, T. palmi would expand their potential distribution to whole Korean peninsula except the alpine region in Gangwon-do and Yanggang-do and Hamgyeongbuk-do in 2070s. Therefore, the monitoring system and management strategy for T. palmi should be reconsidered and re-evaluated.

Thirty-one Campylobacter jejuni isolates (22 from various local sources, 9 from imported chicken meats) were subtyped with PFGE and flaA typing to investigate their genetic relatedness. Based on a 90% similarity criterion, 23 and 21 genotypic patterns were formed by PFGE and flaA typing, respectively. The discriminatory indices for PFGE, flaA typing, and a composite analysis of PFGE and flaA typing were 0.9785, 0.9527, and 0.9871, respectively. Similar patterns in composite analysis were observed between sources (cattle and chicken, and cattle and human), indicating that reservoir animals may have been the source of human campylobacteriosis. Therefore, strict hygiene measures from farm to table should be implemented to prevent diseases due to C. jejuni in humans.

Purpose This study, for the first time attempts to investigate the determinants of the Korean foodservice franchising employing various theories. In addition this study attempts to consider distinguishable foodservice characteristics such as the segment of foodservice and the market demand. This study expects to contribute to the theoretical underpinnings of the foodservice franchising by incorporating distinguishable foodservice characteristics to franchising theories. Method The sample for this study came from uniform franchise offering circular based on Korea Fair Trade Commission. There were 1,598 food service franchisors in uniform franchise offering circular 2009-2011 listing. However, 596 were excluded because of data irregularities remaining 1,002 available for sample. Based on the literature review, we choose seven determinants. The seven determinants were measured as follows. Firm age was measured as two proxy variables. One was measured as the years of franchising business. The other was measured as the years of business. Firm size was measured as total sales. Capital scarcity was measured as the ratio of total debt to total asset. Start-up costs were measured as initial investment. Monitoring costs were measured as the number of major cities franchised units operates. Royalty was measured as sales of a franchised unit. Finally, brand name was measured as advertising expenditures. Results The first proposition (firm age is related to the use of franchising) was supported by the signaling theory and the transaction costs theory instead of the resource scarcity theory. The age (measured as the years of franchising business) had a statistically significant positive relationship with the use of franchising. The second proposition (firm age is related to the use of franchising) was supported by the logic of the resource scarcity. The age (measured as the years of business) had a statistically significant negative relationship with the use of franchising. The third proposition (firm size is related to the use of franchising) was supported by the signaling theory instead of the resource scarcity theory. The firm size had a statistically significant positive relationship with the use of franchising. The fourth proposition (capital scarcity is related to the use of franchising) was not supported by the resource scarcity theory. The capital scarcity had not a statistically significant positive relationship with the use of franchising. The fifth proposition (start-up costs is related to the use of franchising) was not supported. The start-up costs had not a statistically significant either positive or negative relationship with the use of franchising. The sixth proposition (monitoring costs is related to the use of franchising) was supported by the agency theory. The monitoring costs had a statistically significant positive relationship with the use of franchising.. The seventh proposition (royalty is related to the use of franchising) was not supported by the agency theory. The royalty had not a statistically significant positive relationship with the use of franchising. The eighth proposition (brand name is related to the use of franchising) was not supported by the agency theory. Brand name had not a statistically significant negative relationship with the use of franchising. Conclusions Five propositions were found to be statistically significant from eight propositions. The four determinants such as firm age, firm size, monitoring costs, and brand name can affect the use of franchising for the foodservice industry in South Korea. Relating the significant propositions to the diverse theories, there was no one theory that dominated all propositions. The signaling theory was more appropriate for both firm age (the years of franchising business) and firm size while the transaction costs theory was more appropriate for age (the years of franchising business) as well. In addition, the resource scarcity theory was more appropriate for firm age (the years of business). Finally, the agency theory was appropriate for monitoring costs although this theory needed to be ameliorated for brand name.

A full-length lysozyme cDNA from Gryllotalpa orientalis was cloned and sequenced. The deduced amino acid sequence of the lysozyme protein was 143 amino acids in length, with a calculated molecular mass of 15.84 kDa and an isoelectric point of 4.74. Sequence motifs, together with alignment and phylogenetic results, confirmed that G. orientalis lysozyme belongs to the C (chicken)-type lysozyme family of proteins. The protein sequence of lysozyme from G. orientalis showed high identity to that of Drosophila melanogaster (51.7%); however, in contrast to D. melanogaster lysozyme, G. orientalis lysozyme was immune inducible and expressed in a wide range of tissues. Expression of G. orientalis lysozyme mRNA was highest at 8 h post-infection and subsequently decreased with time after bacterial infection. We also expressed G. orientalis lysozyme protein in vitro using the pET expression system. Compared with the negative control, over-expressed G. orientalis lysozyme showed antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus subtilis by radial diffusion assay, with MIC values of 30.3 μM and 7.55 μM respectively. These results indicate that G. orientalis lysozyme may have stronger antimicrobial activity than other lysozymes against a broad range of microorganisms.

Hemocytes are key players in the immune response against pathogens in insects. However, the hemocyte types and their functions in the white-spotted flower chafers, Protaetia brevitarsis seulensis (Kolbe), are not known. In this study, we used various microscopes, molecular probes, and flow cytometric analyses to characterize the hemocytes in Protaetia brevitarsis seulensis. we identified the professional phagocytes, granulocytes (GRs), which mediate encapsulation and phagocytosis of pathogens. The GRs were immunologically or morphologically activated and phagocytosed potentially hazardous substances in vivo.

Transcription activator like effector nucleases (TALENs) are artificial restriction enzymes generated by fusing a TALE DNA binding domain to a DNA cleavage domain which remove and introduce specific genes to produce transgenic animals. To investigate the efficient laboratory techniques for the injection of TALEN mRNA, pEGFP-N1 commercial plasmid were microinjected into porcine parthenogenetic and in vitro fertilization (IVF). In Experiment 1, to investigate injection time, compared 4 different time durations (2 hr, 4 hrs, 6 hrs & 8 hrs) after post activation of parthenogenetic embryos and after 6 hrs of co-incubation with sperms in IVF embryos. There were significant difference (P<0.05) in development to the blastocysts (4.4, 8.9, 3.9, 0.6%), GFP expression in blastocysts (1.3, 5.7, 2.3, 0.0%) which injected after post activation of 4 hrs compared with other 3 groups. IVF embryos after 2 hrs and 4 hrs injected were expressed GFP significantly higher than rest of two groups (P<0.05). In Experiment 2, compared development of 2 different concentrations (20 ng/μl and 50 ng/μl) of EGFP injection. There were significant difference (P<0.05) between two treatments which has higher cleavage (58.8 vs 41.9%), blastocysts development rate (13.0 vs 11.1%) and GFP expressed blastocysts (5.7 vs 0.0%) in 20 ng/μl than the 50 ng/μl in parthenogenetic embryos. In IVF embryos, only 20 ng/μl injected embryos were expressed GFP (4.2%) after 7 days of incubation and 77.3 vs 64.7% of cleavage, 26.4 vs 23.5% development to blastocysts. In Experiment 3, three different volumes (5, 10 and 20 pl) were microinjected into porcine embryos to determine the most appropriate volume. Out of 3 groups, significantly higher development rates of cleavage (68.3, 58.0, 29.3%), blastocysts (11.7, 12.7, 0.5%) and GFP expressed blastocysts (2.9, 7.8, 0.0%) were shown in the 10 pl group (P<0.05). In conclusion, these results imply that 20 ng/μl concentration, 10 pl of volume and injection at 4 hrs after post activation for parthenogenetic and 2∼4 hrs after IVF, 20 ng/μl concentration and 10 pl volume for IVF embryos were more effective microinjection conditions.

Precise, rapid and simple methods for species identification in animals are among the most important techniques in the livestock industry and research fields including meat classification. In this study, polymerase chain reaction (PCR) based molecular identification using inter species polymorphisms were examined by PCR-restriction fragment length polymorphism (RFLP) analysis for mitochondrial DNA (mtDNA) cytochrome b (CYTB) gene sequences among four mammalian livestock animals (cattle, horse, goat and pig). The results from PCR-RFLP analysis using the AluI restriction enzyme were also provided for the species-specific band patterns among CYTB gene sequences in these four species. The AluI-digestion for CYTB genes provided interesting migration patterns differentially displayed according to each species. Cattle and horse had one AluI-recognition site at different nucleotide positions and their AluI-digested fragments showed different band patterns on the gels. Pig had two AluI-recognition sites within the amplified CYTB sequences and produced three bands on the gels. Goat had no AluI-recognition site and was located at the same position as the uncut PCR product. The results showed the species-specific band patterns on a single gel among the four livestock animal species by AluI-RFLP. In addition, the results from blind tests for the meat samples collected from providers without any records showed the identical information on the species recorded by observing their phenotypes before slaughter. The application of this PCR-RFLP method can be useful and provide rapid, simple, and clear information regarding species identification for various tissue samples originating from tested livestock species.

In recent years, there has been developed anti-PID technologies(Potential Induced Degradation) in various levels from solar cell to module and to system to enhance of the long life reliability of photovoltaics(PV) system. Such technologies must economically ensure profits for both manufacturers of solar cells and investors of PV system simultaneously for PV industry development. This paper describes a comparison between and selection from two anti-PID technologies in the solar cell level, ES(modification of emitter structure) and ARC(modification of anti-reflective coating) based on the economic features of anti-PID solar cell production system with 60MW capacity for a solar cell maker and a 1MW PV power plant installed with PV modules using anti-PID solar cells. From the comparison between ES and ARC, it is shown that ARC anti-PID technology can make more profit for both a solar cell maker and a PV power plant investor.

The green pale plant bug, Apolygus spinolae was one of the main insect pests that damaged leaves and fruit in grapes and its damage status was firstly reported in 2000 in grape orchards. This research was conducted to evaluate the distribution and difference in damage rate depending in management type of grapevine orchards (domestic sale farm vs export farm) in the export complex area of Korea (Hwangsung in Gyeonggii, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. Damage by A. spinolae occurred in all 62 survey farms and damage rate differed depending on locality and individual farms in the same area. Damage rate was lower in export farms than in domestic sale farms, and damage rate of leaves was highly correlated with damage rate of new shoots. 15 species of hemipteran insect were attracted to sticky traps and A. spinolae was the dominant species. The attracted number of A. spinolae in the sticky traps differed depending on locality, and more occurred in domestic sale farms than expert farms. A. spinolae was continually attracted to sticky traps in the harvest period in grapevine orchards.

This report is a part of research on pests occurring in grapevine orchards in export complexes (Hwangsung in Gyeonggi, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. This research was conducted to evaluate the distribution and difference in damage rates depending on management types of grapevine orchards (domestic sale farm vs. export farm). Damage by Arbordia spp. occurred only in 2010 and differed depending on localities and individual farms in the same area. Numbers of orchards damaged by Arbordia spp. were one, two and four in Hwasung, Namwon and Sangju, respectively, and the damage rate was below 6.2%. There was no damage in the orchards in 2011 and 2012, however, Arbordia spp. were collected on sticky traps in the orchards. A. nigrigena and A. kakogawana were the dominant species in Yeongcheon and Yeongdong, respectively, in 2011. A. kakogawana, A. maculifrons and A. nigrigena were collected on sticky traps in 2012. Collected numbers of Arbordia spp. were different depending on localities and management types of the orchards (domestic sale vs. export). A. kakogawana was the dominant species in all the survey sites and the densities were higher in the domestic sale farms than in the export farms.

딜에 관한 연구는 대부분이 항산화 물질 및 항암효과 등에 관한 연구가 수행되었으며, 유전적 다양성에 대한 연구는 수행되지 않고 있다. 작물의 유전적 다양성 분석은 자원의 보존, 관리 및 새로운 품종 개발 등에 활용될 수 있는 중요한 정보를제공한다. 본 연구는 Dill 유전자원에 대한 유전적 다양성 분석을 위한 마커 선발을 위해 수행하였다. 1. 유전적 다양성 평가를 위하여 Operon사의 OPA, OPB 그리고 OPC 3 set, 60개 RAPD 마커에 대해서 1차 선발 후, 재현성이 있는 마커를 최종 선발하였다. 선발된 RAPD 마커의 다형성 분석을 위하여 국립농업유전자원센터에서 보유중인 dill 자원 16점을 사용하였다. 2. PCR 산물의 크기가 200 bp에서 3,000 bp 사이에 있는band들을 분석한 결과, 마커 당 평균 7 ~ 14개의 단편들을 나타내었다. 총 band 수는 119개였으며, 그 중 109개가 다형성을보였다. 총 12개의 마커 중 7개의 마커는 모든 band가 다형성을 보였고, 나머지 5개 마커는 70%~ 91%가 다형성 band였다. 각각의 마커에 대한 Nei’s gene diversity(H) 지수는 0.13 ~ 0.28였으며, 평균은 0.214이다. Shannon’s information index(I) 지수는 0.23 ~ 0.44이며, 평균은 0.353이다. 3. UPGMA tree에서는 그루지아 원산 1점을 제외한 15점이 3개 군집으로 나누어졌다. 군집 I은 2점, 군집 II는 11점이 포함되었으며, 군집 III에서는 2점이 포함되었다. 본 실험으로 선발된 RAPD marker들은 dill 유전자원에 대한 유전적 다양성 분석에 활용될 수 있을 것이다.

The mushrooms have been used as traditional medicines and food resources in many countries. The objective of this study was to determine antioxidant compounds and to evoluate tyrosinase inhibitory activity of extracts from Hypsizigus marmoreus. The pileus and stipe of Hypsizigus marmoreus were extracted with methanol and water, separately. The methanol extract of Hypsizigus marmoreus were fractioned by hexane, chloroform, ethylacetate and buthanol. The concentrations of total polyphenolics and flavonoids in methanol extracts were investigated by colorimetric methods. The concentrations of total polyphenolics and flavonoids in methanol extract of the pileus was higher than methanol extract of the stipe. The DPPH redical scavenging activity of the pileus extract was also higher than stipe extract in methanol extract. The IC50 of DPPH redical scavenging activity of the pileus and stipe in methanol extract were 18 mg/ml and 1 10 mg/ml, respectively. The IC50 of tyrosinase inhibitory activity of the pileus and stipe in methanol extract were 500 mg/ml and 1,000 mg/ml in methanol extract. These results suggest that Hypsizigus marmoreus can be potentially used as a source of natural antioxidant agent in the cosmetic industry as well as the food, pharmaceutical and medicinal industry.

Population dynamics of American serpentine leafminer, Liriomyza trifolii, were modeled and simulated to compare the temperature effects of air and tomato leaf inside greenhouse using DYMEX model simulator. The DYMEX model simulator consisted of series of modules with the parameters of temperature dependent development and oviposition model of L. trifolii from pre-published data. Leaf surface temperature of cherry tomato leafs was monitored according to three tomato plant positions using an infrared temperature gun. Air temperature was monitored at same three positions using a self-contained temperature logger. Data sets of observed air temperature and average leaf surface temperatures were prepared, and incorporated into DYMEX simulator to compare the effects of air and leaf surface temperature on population dynamics of L. trifolii. The number of L. trifolii larvae was counted by visual inspection in tomato plants to verify the performance of DYMEX simulation. The egg, pupa, and adult stage of L. trifolii were not counted due to its availability of visual inspection. Based on correlation analysis, L. trifolii was affected greatly by the leaf temperatures, rather than air temperatures.